Restriction map for
pFN21AE9575
(Linear) (Six-base) MAPSORT of: KIEE9575.seq Check: 651 from: 1 to: 1077
KIEE9575.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 529 751 1077
Size: 529 222 326
Fragments arranged by size:
529 326 222
AatII G_ACGT'C
Cuts at: 0 533 1077
Size: 533 544
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1043 1077
Size: 1043 34
AfeI AGC'GCT
Cuts at: 0 361 397 1077
Size: 361 36 680
Fragments arranged by size:
680 361 36
AflIII A'CryG_T
Cuts at: 0 317 818 1077
Size: 317 501 259
Fragments arranged by size:
501 317 259
AleI CACnn'nnGTG
Cuts at: 0 994 1077
Size: 994 83
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1057 1077
Size: 1057 20
AlwNI CAG_nnn'CTG
Cuts at: 0 48 1077
Size: 48 1029
ApaI G_GGCC'C
Cuts at: 0 161 1077
Size: 161 916
ApaLI G'TGCA_C
Cuts at: 0 499 1077
Size: 499 578
ApoI r'AATT_y
Cuts at: 0 895 1077
Size: 895 182
AvaI C'yCGr_G
Cuts at: 0 114 890 1077
Size: 114 776 187
Fragments arranged by size:
776 187 114
BanI G'GyrC_C
Cuts at: 0 401 936 1077
Size: 401 535 141
Fragments arranged by size:
535 401 141
BanII G_rGCy'C
Cuts at: 0 161 1077
Size: 161 916
BbeI G_GCGC'C
Cuts at: 0 405 1077
Size: 405 672
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 994 1028 1077
Size: 994 34 49
Fragments arranged by size:
994 49 34
BclI T'GATC_A
Cuts at: 0 185 620 1077
Size: 185 435 457
Fragments arranged by size:
457 435 185
BglI GCCn_nnn'nGGC
Cuts at: 0 568 1077
Size: 568 509
BlpI GC'TnA_GC
Cuts at: 0 385 1077
Size: 385 692
Bme1580I G_kGCm'C
Cuts at: 0 161 503 941 1077
Size: 161 342 438 136
Fragments arranged by size:
438 342 161 136
BmgBI CAC'GTC
Cuts at: 0 992 1077
Size: 992 85
BmrI ACTGGGnnnn_n'
Cuts at: 0 273 1077
Size: 273 804
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1077
Size: 1039 38
Bpu10I CC'TnA_GC
Cuts at: 0 193 1077
Size: 193 884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 637 1077
Size: 520 117 440
Fragments arranged by size:
520 440 117
BsaAI yAC'GTr
Cuts at: 0 819 1077
Size: 819 258
BsaHI Gr'CG_yC
Cuts at: 0 402 530 1077
Size: 402 128 547
Fragments arranged by size:
547 402 128
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1057 1077
Size: 1057 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 685 1077
Size: 685 392
BseYI C'CCAG_C
Cuts at: 0 553 1077
Size: 553 524
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 1077
Size: 452 625
BsiEI CG_ry'CG
Cuts at: 0 684 1077
Size: 684 393
BsiHKAI G_wGCw'C
Cuts at: 0 503 770 1077
Size: 503 267 307
Fragments arranged by size:
503 307 267
Bsp1286I G_dGCh'C
Cuts at: 0 161 503 770 941 1077
Size: 161 342 267 171 136
Fragments arranged by size:
342 267 171 161 136
BspHI T'CATG_A
Cuts at: 0 182 1077
Size: 182 895
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 529 751 1077
Size: 529 222 326
Fragments arranged by size:
529 326 222
BsrBI CCG'CTC
Cuts at: 0 384 1077
Size: 384 693
BsrDI GCAATG_nn'
Cuts at: 0 246 1077
Size: 246 831
BsrFI r'CCGG_y
Cuts at: 0 19 146 1009 1077
Size: 19 127 863 68
Fragments arranged by size:
863 127 68 19
BsrGI T'GTAC_A
Cuts at: 0 821 1077
Size: 821 256
BssHII G'CGCG_C
Cuts at: 0 475 699 1077
Size: 475 224 378
Fragments arranged by size:
475 378 224
BstAPI GCAn_nnn'nTGC
Cuts at: 0 48 1077
Size: 48 1029
BstEII G'GTnAC_C
Cuts at: 0 294 1077
Size: 294 783
BstYI r'GATC_y
Cuts at: 0 163 1077
Size: 163 914
BtgI C'CryG_G
Cuts at: 0 80 299 626 716 1077
Size: 80 219 327 90 361
Fragments arranged by size:
361 327 219 90 80
DraIII CAC_nnn'GTG
Cuts at: 0 783 836 1077
Size: 783 53 241
Fragments arranged by size:
783 241 53
DrdI GACnn_nn'nnGTC
Cuts at: 0 462 1077
Size: 462 615
EaeI y'GGCC_r
Cuts at: 0 215 422 560 641 681 713 1077
Size: 215 207 138 81 40 32 364
Fragments arranged by size:
364 215 207 138 81 40 32
EagI C'GGCC_G
Cuts at: 0 681 1077
Size: 681 396
EarI CTCTTCn'nnn_
Cuts at: 0 130 586 979 1077
Size: 130 456 393 98
Fragments arranged by size:
456 393 130 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 969 1077
Size: 969 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1043 1077
Size: 1039 4 34
Fragments arranged by size:
1039 34 4
EcoO109I rG'GnC_Cy
Cuts at: 0 6 157 372 437 1077
Size: 6 151 215 65 640
Fragments arranged by size:
640 215 151 65 6
EcoRI G'AATT_C
Cuts at: 0 895 1077
Size: 895 182
FspI TGC'GCA
Cuts at: 0 664 1016 1077
Size: 664 352 61
Fragments arranged by size:
664 352 61
FspAI rTGC'GCAy
Cuts at: 0 1016 1077
Size: 1016 61
HaeII r_GCGC'y
Cuts at: 0 363 399 405 1077
Size: 363 36 6 672
Fragments arranged by size:
672 363 36 6
KasI G'GCGC_C
Cuts at: 0 401 1077
Size: 401 676
MscI TGG'CCA
Cuts at: 0 217 562 1077
Size: 217 345 515
Fragments arranged by size:
515 345 217
MslI CAynn'nnrTG
Cuts at: 0 181 994 1077
Size: 181 813 83
Fragments arranged by size:
813 181 83
MspA1I CmG'CkG
Cuts at: 0 471 693 718 845 903 1077
Size: 471 222 25 127 58 174
Fragments arranged by size:
471 222 174 127 58 25
NaeI GCC'GGC
Cuts at: 0 148 1077
Size: 148 929
NarI GG'CG_CC
Cuts at: 0 402 1077
Size: 402 675
NgoMIV G'CCGG_C
Cuts at: 0 146 1077
Size: 146 931
NspI r_CATG'y
Cuts at: 0 321 1077
Size: 321 756
PasI CC'CwG_GG
Cuts at: 0 507 1077
Size: 507 570
PciI A'CATG_T
Cuts at: 0 317 1077
Size: 317 760
PflMI CCAn_nnn'nTGG
Cuts at: 0 398 1077
Size: 398 679
PmlI CAC'GTG
Cuts at: 0 819 1077
Size: 819 258
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1057 1077
Size: 1057 20
PpuMI rG'GwC_Cy
Cuts at: 0 6 372 437 1077
Size: 6 366 65 640
Fragments arranged by size:
640 366 65 6
PspOMI G'GGCC_C
Cuts at: 0 157 1077
Size: 157 920
PstI C_TGCA'G
Cuts at: 0 44 1077
Size: 44 1033
PvuII CAG'CTG
Cuts at: 0 845 903 1077
Size: 845 58 174
Fragments arranged by size:
845 174 58
SacII CC_GC'GG
Cuts at: 0 719 1077
Size: 719 358
SfcI C'TryA_G
Cuts at: 0 40 1077
Size: 40 1037
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 568 1077
Size: 568 509
SfoI GGC'GCC
Cuts at: 0 403 1077
Size: 403 674
SmaI CCC'GGG
Cuts at: 0 116 892 1077
Size: 116 776 185
Fragments arranged by size:
776 185 116
SmlI C'TyrA_G
Cuts at: 0 535 652 1077
Size: 535 117 425
Fragments arranged by size:
535 425 117
SspI AAT'ATT
Cuts at: 0 919 1077
Size: 919 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 126 162 1077
Size: 126 36 915
Fragments arranged by size:
915 126 36
TatI w'GTAC_w
Cuts at: 0 86 821 1077
Size: 86 735 256
Fragments arranged by size:
735 256 86
Tth111I GACn'n_nGTC
Cuts at: 0 990 1077
Size: 990 87
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 948 1077
Size: 948 129
XmaI C'CCGG_G
Cuts at: 0 114 890 1077
Size: 114 776 187
Fragments arranged by size:
776 187 114
ZraI GAC'GTC
Cuts at: 0 531 1077
Size: 531 546
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AloI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BcgI
BclI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstEII BstYI BtgI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI
HaeII KasI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PmlI PpiI PpuMI PspOMI
PstI PvuII SacII SfcI SfiI SfoI SmaI SmlI
SspI TaqII TatI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AlfI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI
BfrBI BglII BmtI BplI BsaI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BssSI BstBI BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI EcoICRI EcoNI EcoRV
FalI FseI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PmeI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SgfI SgrAI SnaBI SpeI SphI SrfI StuI StyI
SwaI TstI XbaI XhoI XmnI