Restriction map for
pFN21AE9574
(Linear) (Six-base) MAPSORT of: KIEE9574.seq Check: 5819 from: 1 to: 1050
KIEE9574.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 992 1050
Size: 992 58
AflIII A'CryG_T
Cuts at: 0 356 962 1050
Size: 356 606 88
Fragments arranged by size:
606 356 88
AleI CACnn'nnGTG
Cuts at: 0 73 1050
Size: 73 977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 349 1050
Size: 349 701
BanI G'GyrC_C
Cuts at: 0 428 1050
Size: 428 622
BbsI GAAGACnn'nnnn_
Cuts at: 0 132 1042 1050
Size: 132 910 8
Fragments arranged by size:
910 132 8
BciVI GTATCCnnnnn_n'
Cuts at: 0 45 1050
Size: 45 1005
BglI GCCn_nnn'nGGC
Cuts at: 0 454 1050
Size: 454 596
Bme1580I G_kGCm'C
Cuts at: 0 38 1050
Size: 38 1012
BmgBI CAC'GTC
Cuts at: 0 281 686 1050
Size: 281 405 364
Fragments arranged by size:
405 364 281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 823 1050
Size: 823 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1050
Size: 706 344
BsaAI yAC'GTr
Cuts at: 0 900 1050
Size: 900 150
BsaWI w'CCGG_w
Cuts at: 0 680 956 1050
Size: 680 276 94
Fragments arranged by size:
680 276 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 235 265 796 826 982 1012 1050
Size: 235 30 531 30 156 30 38
Fragments arranged by size:
531 235 156 38 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 550 943 1050
Size: 550 393 107
Fragments arranged by size:
550 393 107
BseYI C'CCAG_C
Cuts at: 0 156 946 1050
Size: 156 790 104
Fragments arranged by size:
790 156 104
BsiEI CG_ry'CG
Cuts at: 0 399 1050
Size: 399 651
BsmI GAATG_Cn'
Cuts at: 0 187 1050
Size: 187 863
Bsp1286I G_dGCh'C
Cuts at: 0 38 1050
Size: 38 1012
BspEI T'CCGG_A
Cuts at: 0 680 956 1050
Size: 680 276 94
Fragments arranged by size:
680 276 94
BsrDI GCAATG_nn'
Cuts at: 0 168 1050
Size: 168 882
BsrFI r'CCGG_y
Cuts at: 0 448 907 1050
Size: 448 459 143
Fragments arranged by size:
459 448 143
BsrGI T'GTAC_A
Cuts at: 0 288 1050
Size: 288 762
BtgI C'CryG_G
Cuts at: 0 473 602 980 1050
Size: 473 129 378 70
Fragments arranged by size:
473 378 129 70
BtsI GCAGTG_nn'
Cuts at: 0 743 1050
Size: 743 307
DrdI GACnn_nn'nnGTC
Cuts at: 0 975 1050
Size: 975 75
EaeI y'GGCC_r
Cuts at: 0 380 605 743 905 909 1050
Size: 380 225 138 162 4 141
Fragments arranged by size:
380 225 162 141 138 4
EarI CTCTTCn'nnn_
Cuts at: 0 968 1050
Size: 968 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 823 992 1050
Size: 823 169 58
Fragments arranged by size:
823 169 58
FseI GG_CCGG'CC
Cuts at: 0 911 1050
Size: 911 139
HaeII r_GCGC'y
Cuts at: 0 265 395 1050
Size: 265 130 655
Fragments arranged by size:
655 265 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 796 828 982 1014 1050
Size: 235 32 529 32 154 32 36
Fragments arranged by size:
529 235 154 36 32 32 32
HincII GTy'rAC
Cuts at: 0 968 1050
Size: 968 82
MscI TGG'CCA
Cuts at: 0 382 607 745 1050
Size: 382 225 138 305
Fragments arranged by size:
382 305 225 138
MslI CAynn'nnrTG
Cuts at: 0 41 73 898 1050
Size: 41 32 825 152
Fragments arranged by size:
825 152 41 32
NaeI GCC'GGC
Cuts at: 0 450 909 1050
Size: 450 459 141
Fragments arranged by size:
459 450 141
NgoMIV G'CCGG_C
Cuts at: 0 448 907 1050
Size: 448 459 143
Fragments arranged by size:
459 448 143
NspI r_CATG'y
Cuts at: 0 360 1050
Size: 360 690
PciI A'CATG_T
Cuts at: 0 356 1050
Size: 356 694
PfoI T'CCnGG_A
Cuts at: 0 530 800 1050
Size: 530 270 250
Fragments arranged by size:
530 270 250
PstI C_TGCA'G
Cuts at: 0 328 1050
Size: 328 722
ScaI AGT'ACT
Cuts at: 0 541 1050
Size: 541 509
SexAI A'CCwGG_T
Cuts at: 0 773 1050
Size: 773 277
SfcI C'TryA_G
Cuts at: 0 324 573 1050
Size: 324 249 477
Fragments arranged by size:
477 324 249
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 454 1050
Size: 454 596
SmlI C'TyrA_G
Cuts at: 0 721 1050
Size: 721 329
StyI C'CwwG_G
Cuts at: 0 119 1050
Size: 119 931
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 168 831 1050
Size: 168 663 219
Fragments arranged by size:
663 219 168
TatI w'GTAC_w
Cuts at: 0 288 539 1050
Size: 288 251 511
Fragments arranged by size:
511 288 251
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 453 485 806 835 838 867 1050
Size: 453 32 321 29 3 29 183
Fragments arranged by size:
453 321 183 32 29 29 3
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 307 615 897 1050
Size: 307 308 282 153
Fragments arranged by size:
308 307 282 153
XmnI GAAnn'nnTTC
Cuts at: 0 7 1050
Size: 7 1043
Enzymes that do cut:
AcuI AflIII AleI AlfI BanI BbsI BciVI BglI
Bme1580I BmgBI BpmI BpuEI BsaAI BsaWI BsaXI BseRI
BseYI BsiEI BsmI Bsp1286I BspEI BsrDI BsrFI BsrGI
BtgI BtsI DrdI EaeI EarI Eco57MI FseI HaeII
Hin4I HincII MscI MslI NaeI NgoMIV NspI PciI
PfoI PstI ScaI SexAI SfcI SfiI SmlI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI
BcgI BclI BfrBI BglII BlpI BmrI BmtI BplI
Bpu10I BsaI BsaBI BsaHI BsgI BsiHKAI BsiWI BsmBI
BspHI BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmaI ZraI