Restriction map for
pFN21AE9569
(Linear) (Six-base) MAPSORT of: KIEE9569.seq Check: 2082 from: 1 to: 1161
KIEE9569.orf
With 182 enzymes: SgfI *
November 11, 2011 11:32 ..
AccI GT'mk_AC
Cuts at: 0 653 1161
Size: 653 508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 1161
Size: 564 597
AleI CACnn'nnGTG
Cuts at: 0 133 1161
Size: 133 1028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 316 1161
Size: 316 845
ApaI G_GGCC'C
Cuts at: 0 476 1053 1123 1161
Size: 476 577 70 38
Fragments arranged by size:
577 476 70 38
ApaLI G'TGCA_C
Cuts at: 0 301 1161
Size: 301 860
AscI GG'CGCG_CC
Cuts at: 0 645 684 1161
Size: 645 39 477
Fragments arranged by size:
645 477 39
AvaI C'yCGr_G
Cuts at: 0 9 67 672 954 1090 1161
Size: 9 58 605 282 136 71
Fragments arranged by size:
605 282 136 71 58 9
BanI G'GyrC_C
Cuts at: 0 62 115 676 982 1161
Size: 62 53 561 306 179
Fragments arranged by size:
561 306 179 62 53
BanII G_rGCy'C
Cuts at: 0 443 476 673 1053 1097 1123 1161
Size: 443 33 197 380 44 26 38
Fragments arranged by size:
443 380 197 44 38 33 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 570 1161
Size: 570 591
BfrBI ATG'CAT
Cuts at: 0 1085 1161
Size: 1085 76
BglI GCCn_nnn'nGGC
Cuts at: 0 44 932 1161
Size: 44 888 229
Fragments arranged by size:
888 229 44
Bme1580I G_kGCm'C
Cuts at: 0 86 118 239 305 476 681 942 985
Size: 86 32 121 66 171 205 261 43
Cuts at: 985 1053 1123 1161
Size: 68 70 38
Fragments arranged by size:
261 205 171 121 86 70 68 66
43 38 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 560 1161
Size: 560 601
Bpu10I CC'TnA_GC
Cuts at: 0 481 1161
Size: 481 680
BsaI GGTCTCn'nnnn_
Cuts at: 0 843 888 1161
Size: 843 45 273
Fragments arranged by size:
843 273 45
BsaBI GATnn'nnATC
Cuts at: 0 715 1161
Size: 715 446
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 823 835 853 865 997 1027 1161
Size: 823 12 18 12 132 30 134
Fragments arranged by size:
823 134 132 30 18 12 12
BseYI C'CCAG_C
Cuts at: 0 269 930 1076 1161
Size: 269 661 146 85
Fragments arranged by size:
661 269 146 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 552 1161
Size: 376 176 609
Fragments arranged by size:
609 376 176
BsiHKAI G_wGCw'C
Cuts at: 0 305 1161
Size: 305 856
BsmBI CGTCTCn'nnnn_
Cuts at: 0 12 360 1161
Size: 12 348 801
Fragments arranged by size:
801 348 12
Bsp1286I G_dGCh'C
Cuts at: 0 86 118 239 305 443 476 673 681
Size: 86 32 121 66 138 33 197 8
Cuts at: 681 942 985 1053 1097 1123 1161
Size: 261 43 68 44 26 38
Fragments arranged by size:
261 197 138 121 86 68 66 44
43 38 33 32 26 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 596 1161
Size: 596 565
BsrBI CCG'CTC
Cuts at: 0 795 1161
Size: 795 366
BssHII G'CGCG_C
Cuts at: 0 622 645 684 801 1161
Size: 622 23 39 117 360
Fragments arranged by size:
622 360 117 39 23
BssSI C'ACGA_G
Cuts at: 0 109 1161
Size: 109 1052
BstAPI GCAn_nnn'nTGC
Cuts at: 0 917 1161
Size: 917 244
BtgI C'CryG_G
Cuts at: 0 119 557 665 1161
Size: 119 438 108 496
Fragments arranged by size:
496 438 119 108
EaeI y'GGCC_r
Cuts at: 0 159 206 347 374 506 662 722 783
Size: 159 47 141 27 132 156 60 61
Cuts at: 783 1161
Size: 378
Fragments arranged by size:
378 159 156 141 132 61 60 47
27
EarI CTCTTCn'nnn_
Cuts at: 0 97 469 736 1161
Size: 97 372 267 425
Fragments arranged by size:
425 372 267 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 1161
Size: 564 597
EcoO109I rG'GnC_Cy
Cuts at: 0 473 1049 1050 1119 1161
Size: 473 576 1 69 42
Fragments arranged by size:
576 473 69 42 1
FspI TGC'GCA
Cuts at: 0 409 1161
Size: 409 752
HaeII r_GCGC'y
Cuts at: 0 542 1073 1161
Size: 542 531 88
Fragments arranged by size:
542 531 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 997 1029 1161
Size: 997 32 132
Fragments arranged by size:
997 132 32
HincII GTy'rAC
Cuts at: 0 654 861 1161
Size: 654 207 300
Fragments arranged by size:
654 300 207
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 1161
Size: 983 178
MscI TGG'CCA
Cuts at: 0 376 508 1161
Size: 376 132 653
Fragments arranged by size:
653 376 132
MslI CAynn'nnrTG
Cuts at: 0 133 1161
Size: 133 1028
MspA1I CmG'CkG
Cuts at: 0 390 504 667 1161
Size: 390 114 163 494
Fragments arranged by size:
494 390 163 114
NcoI C'CATG_G
Cuts at: 0 557 1161
Size: 557 604
NsiI A_TGCA'T
Cuts at: 0 1087 1161
Size: 1087 74
PasI CC'CwG_GG
Cuts at: 0 19 1161
Size: 19 1142
PspOMI G'GGCC_C
Cuts at: 0 472 1049 1119 1161
Size: 472 577 70 42
Fragments arranged by size:
577 472 70 42
PspXI vC'TCGA_Gb
Cuts at: 0 1090 1161
Size: 1090 71
PvuII CAG'CTG
Cuts at: 0 504 1161
Size: 504 657
SacII CC_GC'GG
Cuts at: 0 668 1161
Size: 668 493
SalI G'TCGA_C
Cuts at: 0 652 1161
Size: 652 509
SapI GCTCTTCn'nnn_
Cuts at: 0 97 469 736 1161
Size: 97 372 267 425
Fragments arranged by size:
425 372 267 97
SexAI A'CCwGG_T
Cuts at: 0 833 1161
Size: 833 328
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 932 1161
Size: 932 229
SmlI C'TyrA_G
Cuts at: 0 1090 1161
Size: 1090 71
StyI C'CwwG_G
Cuts at: 0 557 1161
Size: 557 604
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 261 1000 1161
Size: 261 739 161
Fragments arranged by size:
739 261 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 695 727 823 855 1161
Size: 695 32 96 32 306
Fragments arranged by size:
695 306 96 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 258 1161
Size: 258 903
XhoI C'TCGA_G
Cuts at: 0 1090 1161
Size: 1090 71
Enzymes that do cut:
AccI AcuI AleI AlfI ApaI ApaLI AscI AvaI
BanI BanII BbsI BfrBI BglI Bme1580I BplI Bpu10I
BsaI BsaBI BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BssHII BssSI BstAPI BtgI EaeI EarI
Eco57MI EcoO109I FspI HaeII Hin4I HincII MmeI MscI
MslI MspA1I NcoI NsiI PasI PspOMI PspXI PvuII
SacII SalI SapI SexAI SfiI SmlI StyI TaqII
TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApoI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BglII BlpI
BmgBI BmrI BmtI BpmI BpuEI BsaAI BsaHI BsaWI
BseRI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrFI
BsrGI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI RsrII SacI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XmaI XmnI
ZraI