(Linear) (Six-base) MAPSORT of: KIEE9568.seq  Check: 4203  from: 1  to: 1194



KIEE9568.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:42  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1168    1194 
   Size:       1168      26
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     656     975    1194 
   Size:         25     631     319     219

  Fragments arranged by size: 

                 631     319     219      25
 
AleI CACnn'nnGTG

Cuts at:      0    1141    1194 
   Size:       1141      53
 
AlwNI CAG_nnn'CTG

Cuts at:      0     208    1194 
   Size:        208     986
 
ApoI r'AATT_y

Cuts at:      0     917     976    1194 
   Size:        917      59     218

  Fragments arranged by size: 

                 917     218      59
 
AseI AT'TA_AT

Cuts at:      0     326    1194 
   Size:        326     868
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1188    1194 
   Size:       1188       6
 
BfrBI ATG'CAT

Cuts at:      0     331    1194 
   Size:        331     863
 
BglII A'GATC_T

Cuts at:      0     545    1194 
   Size:        545     649
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     393    1194 
   Size:        393     801
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     534     861    1194 
   Size:        295     239     327     333

  Fragments arranged by size: 

                 333     327     295     239
 
BsaBI GATnn'nnATC

Cuts at:      0     436    1194 
   Size:        436     758
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     532     562    1194 
   Size:        532      30     632

  Fragments arranged by size: 

                 632     532      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     393    1004    1194 
   Size:        393     611     190

  Fragments arranged by size: 

                 611     393     190
 
BspHI T'CATG_A

Cuts at:      0     149    1194 
   Size:        149    1045
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     777    1041    1168    1194 
   Size:        777     264     127      26

  Fragments arranged by size: 

                 777     264     127      26
 
BssSI C'ACGA_G

Cuts at:      0      47    1194 
   Size:         47    1147
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     920    1194 
   Size:        920     274
 
BstEII G'GTnAC_C

Cuts at:      0      78     789    1194 
   Size:         78     711     405

  Fragments arranged by size: 

                 711     405      78
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     801    1194 
   Size:        801     393
 
BstYI r'GATC_y

Cuts at:      0     492     545     675    1194 
   Size:        492      53     130     519

  Fragments arranged by size: 

                 519     492     130      53
 
BtsI GCAGTG_nn'

Cuts at:      0      59     812    1194 
   Size:         59     753     382

  Fragments arranged by size: 

                 753     382      59
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     589    1194 
   Size:        589     605
 
EaeI y'GGCC_r

Cuts at:      0     227    1194 
   Size:        227     967
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     295     534     656     861     975    1194 
   Size:         25     270     239     122     205     114     219

  Fragments arranged by size: 

                 270     239     219     205     122     114      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0     170    1194 
   Size:        170    1024
 
EcoRV GAT'ATC

Cuts at:      0     530    1107    1194 
   Size:        530     577      87

  Fragments arranged by size: 

                 577     530      87
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     945     977    1194 
   Size:        945      32     217

  Fragments arranged by size: 

                 945     217      32
 
HincII GTy'rAC

Cuts at:      0     864    1194 
   Size:        864     330
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     267    1194 
   Size:        267     927
 
MslI CAynn'nnrTG

Cuts at:      0     796    1141    1194 
   Size:        796     345      53

  Fragments arranged by size: 

                 796     345      53
 
NsiI A_TGCA'T

Cuts at:      0     333    1194 
   Size:        333     861
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     318    1194 
   Size:        318     876
 
PfoI T'CCnGG_A

Cuts at:      0     550    1194 
   Size:        550     644
 
PpuMI rG'GwC_Cy

Cuts at:      0     170    1194 
   Size:        170    1024
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     249     281    1194 
   Size:        249      32     913

  Fragments arranged by size: 

                 913     249      32
 
SexAI A'CCwGG_T

Cuts at:      0      71     188    1194 
   Size:         71     117    1006

  Fragments arranged by size: 

                1006     117      71
 
SfcI C'TryA_G

Cuts at:      0     843    1041    1194 
   Size:        843     198     153

  Fragments arranged by size: 

                 843     198     153
 
SspI AAT'ATT

Cuts at:      0     501    1194 
   Size:        501     693
 
StuI AGG'CCT

Cuts at:      0     236    1194 
   Size:        236     958
 
TatI w'GTAC_w

Cuts at:      0      93    1194 
   Size:         93    1101
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     ApoI     AseI    BciVI    BfrBI
    BglII     BmrI     BpmI    BsaBI    BsaXI     BsgI    BspHI    BspMI
    BssSI   BstAPI   BstEII    BstXI    BstYI     BtsI     DrdI     EaeI
  Eco57MI EcoO109I    EcoRV    Hin4I   HincII     MmeI     MslI     NsiI
    PflMI     PfoI    PpuMI     PsrI    SexAI     SfcI     SspI     StuI
     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI     BclI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BstBI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI