Restriction map for
pFN21AE9567
(Linear) (Six-base) MAPSORT of: KIEE9567.seq Check: 710 from: 1 to: 1464
KIEE9567.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 555 1174 1464
Size: 288 267 619 290
Fragments arranged by size:
619 290 288 267
AfeI AGC'GCT
Cuts at: 0 349 1072 1464
Size: 349 723 392
Fragments arranged by size:
723 392 349
AflIII A'CryG_T
Cuts at: 0 557 1464
Size: 557 907
AleI CACnn'nnGTG
Cuts at: 0 529 1464
Size: 529 935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1255 1464
Size: 1255 209
AlwNI CAG_nnn'CTG
Cuts at: 0 270 1464
Size: 270 1194
ApoI r'AATT_y
Cuts at: 0 1236 1464
Size: 1236 228
AvaI C'yCGr_G
Cuts at: 0 10 256 713 1145 1464
Size: 10 246 457 432 319
Fragments arranged by size:
457 432 319 246 10
BanI G'GyrC_C
Cuts at: 0 670 764 793 1464
Size: 670 94 29 671
Fragments arranged by size:
671 670 94 29
BanII G_rGCy'C
Cuts at: 0 40 220 1411 1464
Size: 40 180 1191 53
Fragments arranged by size:
1191 180 53 40
BbeI G_GCGC'C
Cuts at: 0 768 797 1464
Size: 768 29 667
Fragments arranged by size:
768 667 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 1283 1464
Size: 1283 181
BbvCI CC'TCA_GC
Cuts at: 0 319 751 1464
Size: 319 432 713
Fragments arranged by size:
713 432 319
BglI GCCn_nnn'nGGC
Cuts at: 0 314 1052 1229 1464
Size: 314 738 177 235
Fragments arranged by size:
738 314 235 177
Bme1580I G_kGCm'C
Cuts at: 0 205 673 1464
Size: 205 468 791
Fragments arranged by size:
791 468 205
BmrI ACTGGGnnnn_n'
Cuts at: 0 52 1424 1464
Size: 52 1372 40
Fragments arranged by size:
1372 52 40
Bpu10I CC'TnA_GC
Cuts at: 0 319 751 1464
Size: 319 432 713
Fragments arranged by size:
713 432 319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 1464
Size: 54 1410
BsaI GGTCTCn'nnnn_
Cuts at: 0 475 1306 1464
Size: 475 831 158
Fragments arranged by size:
831 475 158
BsaAI yAC'GTr
Cuts at: 0 236 390 1464
Size: 236 154 1074
Fragments arranged by size:
1074 236 154
BsaBI GATnn'nnATC
Cuts at: 0 82 811 985 1464
Size: 82 729 174 479
Fragments arranged by size:
729 479 174 82
BsaHI Gr'CG_yC
Cuts at: 0 765 794 1464
Size: 765 29 670
Fragments arranged by size:
765 670 29
BsaWI w'CCGG_w
Cuts at: 0 1075 1464
Size: 1075 389
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1117 1147 1464
Size: 1117 30 317
Fragments arranged by size:
1117 317 30
BseYI C'CCAG_C
Cuts at: 0 666 735 1387 1464
Size: 666 69 652 77
Fragments arranged by size:
666 652 77 69
BsiEI CG_ry'CG
Cuts at: 0 45 692 698 1464
Size: 45 647 6 766
Fragments arranged by size:
766 647 45 6
BsiHKAI G_wGCw'C
Cuts at: 0 446 845 1010 1411 1464
Size: 446 399 165 401 53
Fragments arranged by size:
446 401 399 165 53
BsmBI CGTCTCn'nnnn_
Cuts at: 0 145 1464
Size: 145 1319
Bsp1286I G_dGCh'C
Cuts at: 0 40 205 220 446 673 845 1010 1411
Size: 40 165 15 226 227 172 165 401
Cuts at: 1411 1464
Size: 53
Fragments arranged by size:
401 227 226 172 165 165 53 40
15
BspEI T'CCGG_A
Cuts at: 0 1075 1464
Size: 1075 389
BsrBI CCG'CTC
Cuts at: 0 861 1379 1464
Size: 861 518 85
Fragments arranged by size:
861 518 85
BsrDI GCAATG_nn'
Cuts at: 0 821 1464
Size: 821 643
BsrFI r'CCGG_y
Cuts at: 0 44 1464
Size: 44 1420
BssHII G'CGCG_C
Cuts at: 0 452 623 1464
Size: 452 171 841
Fragments arranged by size:
841 452 171
BssSI C'ACGA_G
Cuts at: 0 862 1464
Size: 862 602
BstAPI GCAn_nnn'nTGC
Cuts at: 0 329 430 1464
Size: 329 101 1034
Fragments arranged by size:
1034 329 101
BstEII G'GTnAC_C
Cuts at: 0 222 1464
Size: 222 1242
BstXI CCAn_nnnn'nTGG
Cuts at: 0 503 834 1464
Size: 503 331 630
Fragments arranged by size:
630 503 331
BstYI r'GATC_y
Cuts at: 0 800 1464
Size: 800 664
Bsu36I CC'TnA_GG
Cuts at: 0 1400 1464
Size: 1400 64
BtgI C'CryG_G
Cuts at: 0 692 1464
Size: 692 772
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 213 771 1464
Size: 213 558 693
Fragments arranged by size:
693 558 213
BtsI GCAGTG_nn'
Cuts at: 0 1083 1198 1464
Size: 1083 115 266
Fragments arranged by size:
1083 266 115
DraIII CAC_nnn'GTG
Cuts at: 0 390 506 1464
Size: 390 116 958
Fragments arranged by size:
958 390 116
EaeI y'GGCC_r
Cuts at: 0 110 356 689 695 1464
Size: 110 246 333 6 769
Fragments arranged by size:
769 333 246 110 6
EagI C'GGCC_G
Cuts at: 0 689 695 1464
Size: 689 6 769
Fragments arranged by size:
769 689 6
EarI CTCTTCn'nnn_
Cuts at: 0 304 1045 1329 1464
Size: 304 741 284 135
Fragments arranged by size:
741 304 284 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 15 769 1464
Size: 15 754 695
Fragments arranged by size:
754 695 15
EcoICRI GAG'CTC
Cuts at: 0 1409 1464
Size: 1409 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 555 1174 1464
Size: 288 267 619 290
Fragments arranged by size:
619 290 288 267
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1270 1290 1464
Size: 1270 20 174
Fragments arranged by size:
1270 174 20
EcoO109I rG'GnC_Cy
Cuts at: 0 1058 1464
Size: 1058 406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1124 1464
Size: 1124 340
HaeII r_GCGC'y
Cuts at: 0 351 768 797 1074 1464
Size: 351 417 29 277 390
Fragments arranged by size:
417 390 351 277 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 863 895 1270 1302 1464
Size: 863 32 375 32 162
Fragments arranged by size:
863 375 162 32 32
HincII GTy'rAC
Cuts at: 0 882 1464
Size: 882 582
KasI G'GCGC_C
Cuts at: 0 764 793 1464
Size: 764 29 671
Fragments arranged by size:
764 671 29
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1464
Size: 461 1003
MscI TGG'CCA
Cuts at: 0 112 358 1464
Size: 112 246 1106
Fragments arranged by size:
1106 246 112
MslI CAynn'nnrTG
Cuts at: 0 118 238 529 1464
Size: 118 120 291 935
Fragments arranged by size:
935 291 120 118
MspA1I CmG'CkG
Cuts at: 0 270 694 727 1464
Size: 270 424 33 737
Fragments arranged by size:
737 424 270 33
NarI GG'CG_CC
Cuts at: 0 765 794 1464
Size: 765 29 670
Fragments arranged by size:
765 670 29
NotI GC'GGCC_GC
Cuts at: 0 689 695 1464
Size: 689 6 769
Fragments arranged by size:
769 689 6
NspI r_CATG'y
Cuts at: 0 561 639 1464
Size: 561 78 825
Fragments arranged by size:
825 561 78
PciI A'CATG_T
Cuts at: 0 557 1464
Size: 557 907
PflMI CCAn_nnn'nTGG
Cuts at: 0 390 1464
Size: 390 1074
PpuMI rG'GwC_Cy
Cuts at: 0 1058 1464
Size: 1058 406
PstI C_TGCA'G
Cuts at: 0 491 1464
Size: 491 973
SacI G_AGCT'C
Cuts at: 0 1411 1464
Size: 1411 53
SacII CC_GC'GG
Cuts at: 0 695 1464
Size: 695 769
SanDI GG'GwC_CC
Cuts at: 0 1058 1464
Size: 1058 406
SapI GCTCTTCn'nnn_
Cuts at: 0 304 1464
Size: 304 1160
SexAI A'CCwGG_T
Cuts at: 0 104 502 1464
Size: 104 398 962
Fragments arranged by size:
962 398 104
SfcI C'TryA_G
Cuts at: 0 378 487 1464
Size: 378 109 977
Fragments arranged by size:
977 378 109
SfoI GGC'GCC
Cuts at: 0 766 795 1464
Size: 766 29 669
Fragments arranged by size:
766 669 29
SmaI CCC'GGG
Cuts at: 0 12 258 715 1147 1464
Size: 12 246 457 432 317
Fragments arranged by size:
457 432 317 246 12
SmlI C'TyrA_G
Cuts at: 0 33 1464
Size: 33 1431
SphI G_CATG'C
Cuts at: 0 639 1464
Size: 639 825
StyI C'CwwG_G
Cuts at: 0 1224 1464
Size: 1224 240
TatI w'GTAC_w
Cuts at: 0 278 1168 1464
Size: 278 890 296
Fragments arranged by size:
890 296 278
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1117 1149 1464
Size: 1117 32 315
Fragments arranged by size:
1117 315 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 834 861 1464
Size: 834 27 603
Fragments arranged by size:
834 603 27
XmaI C'CCGG_G
Cuts at: 0 10 256 713 1145 1464
Size: 10 246 457 432 319
Fragments arranged by size:
457 432 319 246 10
XmnI GAAnn'nnTTC
Cuts at: 0 951 1464
Size: 951 513
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI AlwNI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BglI Bme1580I BmrI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NarI NotI NspI
PciI PflMI PpuMI PstI SacI SacII SanDI SapI
SexAI SfcI SfoI SmaI SmlI SphI StyI TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BpmI BseRI BsgI BsiWI BsmI BspHI
BspMI BsrGI BstBI BstZ17I ClaI CspCI DraI DrdI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NaeI NcoI NdeI NgoMIV NheI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SalI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XhoI ZraI