(Linear) (Six-base) MAPSORT of: KIEE9566.seq  Check: 1188  from: 1  to: 1059



KIEE9566.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:42  ..

AflIII A'CryG_T

Cuts at:      0     383     629     896    1059 
   Size:        383     246     267     163

  Fragments arranged by size: 

                 383     267     246     163
 
ApoI r'AATT_y

Cuts at:      0     178    1059 
   Size:        178     881
 
AvaI C'yCGr_G

Cuts at:      0     759    1059 
   Size:        759     300
 
BanI G'GyrC_C

Cuts at:      0     904    1059 
   Size:        904     155
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     721    1059 
   Size:        721     338
 
BbvCI CC'TCA_GC

Cuts at:      0     982    1059 
   Size:        982      77
 
BclI T'GATC_A

Cuts at:      0     164    1059 
   Size:        164     895
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     268     502    1059 
   Size:        268     234     557

  Fragments arranged by size: 

                 557     268     234
 
Bpu10I CC'TnA_GC

Cuts at:      0     982    1059 
   Size:        982      77
 
BsaAI yAC'GTr

Cuts at:      0     630    1059 
   Size:        630     429
 
BsaHI Gr'CG_yC

Cuts at:      0     954    1059 
   Size:        954     105
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     769    1059 
   Size:        769     290
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     832    1055    1059 
   Size:        832     223       4

  Fragments arranged by size: 

                 832     223       4
 
BspHI T'CATG_A

Cuts at:      0     392    1059 
   Size:        392     667
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     626    1059 
   Size:        626     433
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     360     474     588    1059 
   Size:        360     114     114     471

  Fragments arranged by size: 

                 471     360     114     114
 
BstYI r'GATC_y

Cuts at:      0     623    1059 
   Size:        623     436
 
BtgI C'CryG_G

Cuts at:      0     371    1059 
   Size:        371     688
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     138     447    1059 
   Size:        138     309     612

  Fragments arranged by size: 

                 612     309     138
 
BtsI GCAGTG_nn'

Cuts at:      0     167     436     808    1059 
   Size:        167     269     372     251

  Fragments arranged by size: 

                 372     269     251     167
 
EarI CTCTTCn'nnn_

Cuts at:      0     534    1059 
   Size:        534     525
 
HaeII r_GCGC'y

Cuts at:      0     847    1059 
   Size:        847     212
 
HincII GTy'rAC

Cuts at:      0     297    1059 
   Size:        297     762
 
HindIII A'AGCT_T

Cuts at:      0      85    1059 
   Size:         85     974
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     605    1059 
   Size:        605     454
 
MslI CAynn'nnrTG

Cuts at:      0     397     472     607     671    1059 
   Size:        397      75     135      64     388

  Fragments arranged by size: 

                 397     388     135      75      64
 
MspA1I CmG'CkG

Cuts at:      0     302    1059 
   Size:        302     757
 
NspI r_CATG'y

Cuts at:      0     387     820     900    1059 
   Size:        387     433      80     159

  Fragments arranged by size: 

                 433     387     159      80
 
PciI A'CATG_T

Cuts at:      0     383     896    1059 
   Size:        383     513     163

  Fragments arranged by size: 

                 513     383     163
 
PsiI TTA'TAA

Cuts at:      0      41    1059 
   Size:         41    1018
 
PstI C_TGCA'G

Cuts at:      0     799    1059 
   Size:        799     260
 
SapI GCTCTTCn'nnn_

Cuts at:      0     534    1059 
   Size:        534     525
 
SfcI C'TryA_G

Cuts at:      0     795    1059 
   Size:        795     264
 
SmaI CCC'GGG

Cuts at:      0     761    1059 
   Size:        761     298
 
StyI C'CwwG_G

Cuts at:      0    1029    1059 
   Size:       1029      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     714    1059 
   Size:        714     345
 
TatI w'GTAC_w

Cuts at:      0    1007    1059 
   Size:       1007      52
 
Tth111I GACn'n_nGTC

Cuts at:      0     576    1059 
   Size:        576     483
 
XmaI C'CCGG_G

Cuts at:      0     759    1059 
   Size:        759     300
 

 Enzymes that do cut:

   AflIII     ApoI     AvaI     BanI     BbsI    BbvCI     BclI     BmrI
   Bpu10I    BsaAI    BsaHI    BseRI     BsgI    BspHI    BspMI    BstXI
    BstYI     BtgI    BtgZI     BtsI     EarI    HaeII   HincII  HindIII
     MmeI     MslI   MspA1I     NspI     PciI     PsiI     PstI     SapI
     SfcI     SmaI     StyI    TaqII     TatI  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaWI    BsaXI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
     XbaI     XcmI     XhoI     XmnI     ZraI