Restriction map for
pFN21AE9564
(Linear) (Six-base) MAPSORT of: KIEE9564.seq Check: 6609 from: 1 to: 1206
KIEE9564.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AfeI AGC'GCT
Cuts at: 0 403 1206
Size: 403 803
AflIII A'CryG_T
Cuts at: 0 74 1206
Size: 74 1132
AlwNI CAG_nnn'CTG
Cuts at: 0 603 1035 1206
Size: 603 432 171
Fragments arranged by size:
603 432 171
ApaI G_GGCC'C
Cuts at: 0 17 1206
Size: 17 1189
AvaI C'yCGr_G
Cuts at: 0 270 549 582 1101 1206
Size: 270 279 33 519 105
Fragments arranged by size:
519 279 270 105 33
BanI G'GyrC_C
Cuts at: 0 57 136 593 1206
Size: 57 79 457 613
Fragments arranged by size:
613 457 79 57
BanII G_rGCy'C
Cuts at: 0 7 17 864 934 976 1206
Size: 7 10 847 70 42 230
Fragments arranged by size:
847 230 70 42 10 7
BbeI G_GCGC'C
Cuts at: 0 61 140 597 1206
Size: 61 79 457 609
Fragments arranged by size:
609 457 79 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 345 1206
Size: 345 861
BbvCI CC'TCA_GC
Cuts at: 0 1195 1206
Size: 1195 11
BglI GCCn_nnn'nGGC
Cuts at: 0 35 322 481 490 1159 1206
Size: 35 287 159 9 669 47
Fragments arranged by size:
669 287 159 47 35 9
BlpI GC'TnA_GC
Cuts at: 0 670 1206
Size: 670 536
Bme1580I G_kGCm'C
Cuts at: 0 17 370 556 1206
Size: 17 353 186 650
Fragments arranged by size:
650 353 186 17
BmgBI CAC'GTC
Cuts at: 0 77 876 1206
Size: 77 799 330
Fragments arranged by size:
799 330 77
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1182 1206
Size: 1182 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 948 990 1206
Size: 948 42 216
Fragments arranged by size:
948 216 42
Bpu10I CC'TnA_GC
Cuts at: 0 25 1195 1206
Size: 25 1170 11
Fragments arranged by size:
1170 25 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 450 1206
Size: 450 756
BsaAI yAC'GTr
Cuts at: 0 264 557 1206
Size: 264 293 649
Fragments arranged by size:
649 293 264
BsaHI Gr'CG_yC
Cuts at: 0 58 137 195 413 594 705 750 1206
Size: 58 79 58 218 181 111 45 456
Fragments arranged by size:
456 218 181 111 79 58 58 45
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 634 1206
Size: 634 572
BseYI C'CCAG_C
Cuts at: 0 132 378 755 1134 1206
Size: 132 246 377 379 72
Fragments arranged by size:
379 377 246 132 72
BsiEI CG_ry'CG
Cuts at: 0 83 209 317 424 452 1206
Size: 83 126 108 107 28 754
Fragments arranged by size:
754 126 108 107 83 28
BsiHKAI G_wGCw'C
Cuts at: 0 864 976 1206
Size: 864 112 230
Fragments arranged by size:
864 230 112
Bsp1286I G_dGCh'C
Cuts at: 0 7 17 370 556 864 934 976 1206
Size: 7 10 353 186 308 70 42 230
Fragments arranged by size:
353 308 230 186 70 42 10 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 158 1206
Size: 158 1048
BsrBI CCG'CTC
Cuts at: 0 203 710 776 1206
Size: 203 507 66 430
Fragments arranged by size:
507 430 203 66
BsrFI r'CCGG_y
Cuts at: 0 316 746 1206
Size: 316 430 460
Fragments arranged by size:
460 430 316
BsrGI T'GTAC_A
Cuts at: 0 293 1049 1206
Size: 293 756 157
Fragments arranged by size:
756 293 157
BssHII G'CGCG_C
Cuts at: 0 275 306 458 460 520 1206
Size: 275 31 152 2 60 686
Fragments arranged by size:
686 275 152 60 31 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 560 607 1206
Size: 560 47 599
Fragments arranged by size:
599 560 47
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 153 240 252 1206
Size: 132 21 87 12 954
Fragments arranged by size:
954 132 87 21 12
BstYI r'GATC_y
Cuts at: 0 1031 1206
Size: 1031 175
Bsu36I CC'TnA_GG
Cuts at: 0 732 1206
Size: 732 474
BtgI C'CryG_G
Cuts at: 0 179 395 476 485 500 1127 1206
Size: 179 216 81 9 15 627 79
Fragments arranged by size:
627 216 179 81 79 15 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 789 1206
Size: 789 417
BtsI GCAGTG_nn'
Cuts at: 0 296 601 1206
Size: 296 305 605
Fragments arranged by size:
605 305 296
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 807 842 1206
Size: 807 35 364
Fragments arranged by size:
807 364 35
DraIII CAC_nnn'GTG
Cuts at: 0 560 876 1206
Size: 560 316 330
Fragments arranged by size:
560 330 316
EaeI y'GGCC_r
Cuts at: 0 80 206 230 242 314 392 421 473
Size: 80 126 24 12 72 78 29 52
Cuts at: 473 482 497 956 991 1004 1206
Size: 9 15 459 35 13 202
Fragments arranged by size:
459 202 126 80 78 72 52 35
29 24 15 13 12 9
EagI C'GGCC_G
Cuts at: 0 80 206 314 421 1206
Size: 80 126 108 107 785
Fragments arranged by size:
785 126 108 107 80
EarI CTCTTCn'nnn_
Cuts at: 0 622 1206
Size: 622 584
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 774 783 1206
Size: 774 9 423
Fragments arranged by size:
774 423 9
EcoICRI GAG'CTC
Cuts at: 0 862 974 1206
Size: 862 112 232
Fragments arranged by size:
862 232 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 948 990 1206
Size: 948 42 216
Fragments arranged by size:
948 216 42
EcoO109I rG'GnC_Cy
Cuts at: 0 14 66 323 577 736 1206
Size: 14 52 257 254 159 470
Fragments arranged by size:
470 257 254 159 52 14
FspI TGC'GCA
Cuts at: 0 1174 1206
Size: 1174 32
HaeII r_GCGC'y
Cuts at: 0 61 101 140 160 166 405 472 508
Size: 61 40 39 20 6 239 67 36
Cuts at: 508 597 955 1154 1206
Size: 89 358 199 52
Fragments arranged by size:
358 239 199 89 67 61 52 40
39 36 20 6
KasI G'GCGC_C
Cuts at: 0 57 136 593 1206
Size: 57 79 457 613
Fragments arranged by size:
613 457 79 57
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 1206
Size: 170 1036
MscI TGG'CCA
Cuts at: 0 232 244 1206
Size: 232 12 962
Fragments arranged by size:
962 232 12
MslI CAynn'nnrTG
Cuts at: 0 394 910 1206
Size: 394 516 296
Fragments arranged by size:
516 394 296
MspA1I CmG'CkG
Cuts at: 0 181 372 426 478 487 516 674 698
Size: 181 191 54 52 9 29 158 24
Cuts at: 698 845 990 1129 1190 1206
Size: 147 145 139 61 16
Fragments arranged by size:
191 181 158 147 145 139 61 54
52 29 24 16 9
NaeI GCC'GGC
Cuts at: 0 318 748 1206
Size: 318 430 458
Fragments arranged by size:
458 430 318
NarI GG'CG_CC
Cuts at: 0 58 137 594 1206
Size: 58 79 457 612
Fragments arranged by size:
612 457 79 58
NgoMIV G'CCGG_C
Cuts at: 0 316 746 1206
Size: 316 430 460
Fragments arranged by size:
460 430 316
NotI GC'GGCC_GC
Cuts at: 0 421 1206
Size: 421 785
NspI r_CATG'y
Cuts at: 0 53 1206
Size: 53 1153
PasI CC'CwG_GG
Cuts at: 0 62 1206
Size: 62 1144
PflMI CCAn_nnn'nTGG
Cuts at: 0 336 876 1035 1206
Size: 336 540 159 171
Fragments arranged by size:
540 336 171 159
PfoI T'CCnGG_A
Cuts at: 0 1021 1034 1206
Size: 1021 13 172
Fragments arranged by size:
1021 172 13
PmlI CAC'GTG
Cuts at: 0 264 557 1206
Size: 264 293 649
Fragments arranged by size:
649 293 264
PpuMI rG'GwC_Cy
Cuts at: 0 66 577 1206
Size: 66 511 629
Fragments arranged by size:
629 511 66
PspOMI G'GGCC_C
Cuts at: 0 13 1206
Size: 13 1193
PstI C_TGCA'G
Cuts at: 0 545 989 1206
Size: 545 444 217
Fragments arranged by size:
545 444 217
SacI G_AGCT'C
Cuts at: 0 864 976 1206
Size: 864 112 230
Fragments arranged by size:
864 230 112
SacII CC_GC'GG
Cuts at: 0 182 479 488 1130 1206
Size: 182 297 9 642 76
Fragments arranged by size:
642 297 182 76 9
SanDI GG'GwC_CC
Cuts at: 0 66 577 1206
Size: 66 511 629
Fragments arranged by size:
629 511 66
SfcI C'TryA_G
Cuts at: 0 541 985 1206
Size: 541 444 221
Fragments arranged by size:
541 444 221
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 322 481 490 1206
Size: 322 159 9 716
Fragments arranged by size:
716 322 159 9
SfoI GGC'GCC
Cuts at: 0 59 138 595 1206
Size: 59 79 457 611
Fragments arranged by size:
611 457 79 59
SmaI CCC'GGG
Cuts at: 0 272 551 584 1206
Size: 272 279 33 622
Fragments arranged by size:
622 279 272 33
StyI C'CwwG_G
Cuts at: 0 494 1042 1115 1206
Size: 494 548 73 91
Fragments arranged by size:
548 494 91 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 438 1035 1206
Size: 438 597 171
Fragments arranged by size:
597 438 171
TatI w'GTAC_w
Cuts at: 0 293 1049 1206
Size: 293 756 157
Fragments arranged by size:
756 293 157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 630 1206
Size: 630 576
XmaI C'CCGG_G
Cuts at: 0 270 549 582 1206
Size: 270 279 33 624
Fragments arranged by size:
624 279 270 33
Enzymes that do cut:
AfeI AflIII AlwNI ApaI AvaI BanI BanII BbeI
BbsI BbvCI BglI BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NotI NspI PasI PflMI PfoI PmlI PpuMI PspOMI
PstI SacI SacII SanDI SfcI SfiI SfoI SmaI
StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BglII BmrI BmtI BpuEI BsaBI BsaWI BsaXI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BssSI BstBI
BstEII BstZ17I ClaI DraI DrdI EcoNI EcoRI EcoRV
FalI FseI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NruI NsiI PacI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SalI SapI SbfI ScaI SexAI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI XmnI ZraI