Restriction map for
pFN21AE9563
(Linear) (Six-base) MAPSORT of: KIEE9563.seq Check: 3693 from: 1 to: 1437
KIEE9563.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AatII G_ACGT'C
Cuts at: 0 488 1437
Size: 488 949
Acc65I G'GTAC_C
Cuts at: 0 539 1437
Size: 539 898
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1276 1437
Size: 1276 161
AgeI A'CCGG_T
Cuts at: 0 1341 1437
Size: 1341 96
AvaI C'yCGr_G
Cuts at: 0 836 1437
Size: 836 601
BanI G'GyrC_C
Cuts at: 0 41 106 132 168 539 1437
Size: 41 65 26 36 371 898
Fragments arranged by size:
898 371 65 41 36 26
BanII G_rGCy'C
Cuts at: 0 97 127 666 1437
Size: 97 30 539 771
Fragments arranged by size:
771 539 97 30
BbeI G_GCGC'C
Cuts at: 0 110 136 172 1437
Size: 110 26 36 1265
Fragments arranged by size:
1265 110 36 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 462 1437
Size: 462 975
BbvCI CC'TCA_GC
Cuts at: 0 1294 1437
Size: 1294 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 896 930 1437
Size: 896 34 507
Fragments arranged by size:
896 507 34
BclI T'GATC_A
Cuts at: 0 524 695 1437
Size: 524 171 742
Fragments arranged by size:
742 524 171
BfrBI ATG'CAT
Cuts at: 0 593 1245 1437
Size: 593 652 192
Fragments arranged by size:
652 593 192
BglI GCCn_nnn'nGGC
Cuts at: 0 166 1437
Size: 166 1271
Bme1580I G_kGCm'C
Cuts at: 0 44 1437
Size: 44 1393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 1180 1352 1437
Size: 868 312 172 85
Fragments arranged by size:
868 312 172 85
Bpu10I CC'TnA_GC
Cuts at: 0 1294 1437
Size: 1294 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 910 1437
Size: 111 799 527
Fragments arranged by size:
799 527 111
BsaI GGTCTCn'nnnn_
Cuts at: 0 1272 1317 1437
Size: 1272 45 120
Fragments arranged by size:
1272 120 45
BsaHI Gr'CG_yC
Cuts at: 0 107 133 169 485 1437
Size: 107 26 36 316 952
Fragments arranged by size:
952 316 107 36 26
BsaWI w'CCGG_w
Cuts at: 0 1208 1220 1341 1437
Size: 1208 12 121 96
Fragments arranged by size:
1208 121 96 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 630 660 1437
Size: 184 30 416 30 777
Fragments arranged by size:
777 416 184 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 913 1024 1437
Size: 913 111 413
Fragments arranged by size:
913 413 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 1287 1437
Size: 480 807 150
Fragments arranged by size:
807 480 150
BsiEI CG_ry'CG
Cuts at: 0 24 119 287 1437
Size: 24 95 168 1150
Fragments arranged by size:
1150 168 95 24
Bsp1286I G_dGCh'C
Cuts at: 0 44 97 127 666 1437
Size: 44 53 30 539 771
Fragments arranged by size:
771 539 53 44 30
BspHI T'CATG_A
Cuts at: 0 509 1424 1431 1437
Size: 509 915 7 6
Fragments arranged by size:
915 509 7 6
BsrBI CCG'CTC
Cuts at: 0 50 1437
Size: 50 1387
BsrFI r'CCGG_y
Cuts at: 0 1341 1437
Size: 1341 96
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1070 1437
Size: 1070 367
BstXI CCAn_nnnn'nTGG
Cuts at: 0 709 984 1437
Size: 709 275 453
Fragments arranged by size:
709 453 275
BstYI r'GATC_y
Cuts at: 0 443 1437
Size: 443 994
Bsu36I CC'TnA_GG
Cuts at: 0 1171 1437
Size: 1171 266
BtgI C'CryG_G
Cuts at: 0 479 1437
Size: 479 958
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 459 843 1437
Size: 459 384 594
Fragments arranged by size:
594 459 384
BtsI GCAGTG_nn'
Cuts at: 0 740 1437
Size: 740 697
EaeI y'GGCC_r
Cuts at: 0 21 374 1437
Size: 21 353 1063
Fragments arranged by size:
1063 353 21
EagI C'GGCC_G
Cuts at: 0 21 1437
Size: 21 1416
EarI CTCTTCn'nnn_
Cuts at: 0 796 893 1437
Size: 796 97 544
Fragments arranged by size:
796 544 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 325 618 1437
Size: 325 293 819
Fragments arranged by size:
819 325 293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 1180 1276 1352 1437
Size: 868 312 96 76 85
Fragments arranged by size:
868 312 96 85 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1305 1437
Size: 1305 132
EcoO109I rG'GnC_Cy
Cuts at: 0 557 1001 1167 1298 1437
Size: 557 444 166 131 139
Fragments arranged by size:
557 444 166 139 131
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 462 708 1437
Size: 462 246 729
Fragments arranged by size:
729 462 246
HaeII r_GCGC'y
Cuts at: 0 110 136 172 373 1437
Size: 110 26 36 201 1064
Fragments arranged by size:
1064 201 110 36 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 1040 1072 1167 1199 1437
Size: 184 32 824 32 95 32 238
Fragments arranged by size:
824 238 184 95 32 32 32
HincII GTy'rAC
Cuts at: 0 12 1437
Size: 12 1425
HindIII A'AGCT_T
Cuts at: 0 1258 1437
Size: 1258 179
HpaI GTT'AAC
Cuts at: 0 12 1437
Size: 12 1425
KasI G'GCGC_C
Cuts at: 0 106 132 168 1437
Size: 106 26 36 1269
Fragments arranged by size:
1269 106 36 26
KpnI G_GTAC'C
Cuts at: 0 543 1437
Size: 543 894
MfeI C'AATT_G
Cuts at: 0 1380 1437
Size: 1380 57
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 1437
Size: 371 1066
MslI CAynn'nnrTG
Cuts at: 0 478 1107 1437
Size: 478 629 330
Fragments arranged by size:
629 478 330
MspA1I CmG'CkG
Cuts at: 0 20 1076 1234 1437
Size: 20 1056 158 203
Fragments arranged by size:
1056 203 158 20
NarI GG'CG_CC
Cuts at: 0 107 133 169 1437
Size: 107 26 36 1268
Fragments arranged by size:
1268 107 36 26
NcoI C'CATG_G
Cuts at: 0 479 1437
Size: 479 958
NotI GC'GGCC_GC
Cuts at: 0 21 1437
Size: 21 1416
NsiI A_TGCA'T
Cuts at: 0 595 1247 1437
Size: 595 652 190
Fragments arranged by size:
652 595 190
NspI r_CATG'y
Cuts at: 0 1245 1437
Size: 1245 192
PasI CC'CwG_GG
Cuts at: 0 984 1437
Size: 984 453
PflMI CCAn_nnn'nTGG
Cuts at: 0 655 1437
Size: 655 782
PpuMI rG'GwC_Cy
Cuts at: 0 1167 1437
Size: 1167 270
PspXI vC'TCGA_Gb
Cuts at: 0 836 1437
Size: 836 601
PstI C_TGCA'G
Cuts at: 0 1075 1239 1437
Size: 1075 164 198
Fragments arranged by size:
1075 198 164
PvuI CG_AT'CG
Cuts at: 0 287 1437
Size: 287 1150
PvuII CAG'CTG
Cuts at: 0 1076 1234 1437
Size: 1076 158 203
Fragments arranged by size:
1076 203 158
SfcI C'TryA_G
Cuts at: 0 1071 1235 1409 1437
Size: 1071 164 174 28
Fragments arranged by size:
1071 174 164 28
SfoI GGC'GCC
Cuts at: 0 108 134 170 1437
Size: 108 26 36 1267
Fragments arranged by size:
1267 108 36 26
SmlI C'TyrA_G
Cuts at: 0 90 836 925 1437
Size: 90 746 89 512
Fragments arranged by size:
746 512 90 89
SphI G_CATG'C
Cuts at: 0 1245 1437
Size: 1245 192
StyI C'CwwG_G
Cuts at: 0 479 543 1437
Size: 479 64 894
Fragments arranged by size:
894 479 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1080 1344 1437
Size: 1080 264 93
Fragments arranged by size:
1080 264 93
TatI w'GTAC_w
Cuts at: 0 773 1437
Size: 773 664
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 475 507 630 662 1437
Size: 475 32 123 32 775
Fragments arranged by size:
775 475 123 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 115 853 1437
Size: 115 738 584
Fragments arranged by size:
738 584 115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 656 1437
Size: 656 781
XhoI C'TCGA_G
Cuts at: 0 836 1437
Size: 836 601
ZraI GAC'GTC
Cuts at: 0 486 1437
Size: 486 951
Enzymes that do cut:
AatII Acc65I AcuI AgeI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BclI BfrBI BglI Bme1580I BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BsgI
BsiEI Bsp1286I BspHI BsrBI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MmeI MslI MspA1I NarI
NcoI NotI NsiI NspI PasI PflMI PpuMI PspXI
PstI PvuI PvuII SfcI SfoI SmlI SphI StyI
TaqII TatI TstI Tth111I XcmI XhoI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BciVI BglII BlpI BmgBI
BmrI BmtI BplI BsaAI BsaBI BseYI BsiHKAI BsiWI
BsmI BsmBI BspEI BspMI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI
EcoICRI EcoRI EcoRV FseI FspI FspAI MluI MscI
NaeI NdeI NgoMIV NheI NruI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PsrI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI XbaI XmaI XmnI