Restriction map for
pFN21AE9561
(Linear) (Six-base) MAPSORT of: KIEE9561.seq Check: 9059 from: 1 to: 1104
KIEE9561.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 305 1104
Size: 305 799
AccI GT'mk_AC
Cuts at: 0 869 1104
Size: 869 235
Acc65I G'GTAC_C
Cuts at: 0 359 1104
Size: 359 745
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 777 1104
Size: 777 327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 307 1104
Size: 307 797
AlwNI CAG_nnn'CTG
Cuts at: 0 61 1104
Size: 61 1043
ApaI G_GGCC'C
Cuts at: 0 276 988 1051 1104
Size: 276 712 63 53
Fragments arranged by size:
712 276 63 53
ApaLI G'TGCA_C
Cuts at: 0 292 1104
Size: 292 812
AscI GG'CGCG_CC
Cuts at: 0 702 1104
Size: 702 402
AvaI C'yCGr_G
Cuts at: 0 138 981 1091 1104
Size: 138 843 110 13
Fragments arranged by size:
843 138 110 13
BanI G'GyrC_C
Cuts at: 0 359 374 462 484 1104
Size: 359 15 88 22 620
Fragments arranged by size:
620 359 88 22 15
BanII G_rGCy'C
Cuts at: 0 276 512 988 1051 1058 1104
Size: 276 236 476 63 7 46
Fragments arranged by size:
476 276 236 63 46 7
BbeI G_GCGC'C
Cuts at: 0 466 1104
Size: 466 638
BbvCI CC'TCA_GC
Cuts at: 0 472 1104
Size: 472 632
BglI GCCn_nnn'nGGC
Cuts at: 0 712 992 1104
Size: 712 280 112
Fragments arranged by size:
712 280 112
Bme1580I G_kGCm'C
Cuts at: 0 94 230 276 296 521 948 988 1051
Size: 94 136 46 20 225 427 40 63
Cuts at: 1051 1104
Size: 53
Fragments arranged by size:
427 225 136 94 63 53 46 40
20
BmgBI CAC'GTC
Cuts at: 0 555 1104
Size: 555 549
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1104
Size: 526 578
Bpu10I CC'TnA_GC
Cuts at: 0 472 1104
Size: 472 632
BsaHI Gr'CG_yC
Cuts at: 0 463 933 1007 1104
Size: 463 470 74 97
Fragments arranged by size:
470 463 97 74
BsaWI w'CCGG_w
Cuts at: 0 1098 1104
Size: 1098 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 832 862 1104
Size: 832 30 242
Fragments arranged by size:
832 242 30
BseYI C'CCAG_C
Cuts at: 0 87 740 1104
Size: 87 653 364
Fragments arranged by size:
653 364 87
BsiEI CG_ry'CG
Cuts at: 0 668 783 927 1104
Size: 668 115 144 177
Fragments arranged by size:
668 177 144 115
BsiHKAI G_wGCw'C
Cuts at: 0 125 296 512 1104
Size: 125 171 216 592
Fragments arranged by size:
592 216 171 125
BsmBI CGTCTCn'nnnn_
Cuts at: 0 998 1104
Size: 998 106
Bsp1286I G_dGCh'C
Cuts at: 0 94 125 230 276 296 512 521 948
Size: 94 31 105 46 20 216 9 427
Cuts at: 948 988 1051 1058 1104
Size: 40 63 7 46
Fragments arranged by size:
427 216 105 94 63 46 46 40
31 20 9 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 305 587 1104
Size: 305 282 517
Fragments arranged by size:
517 305 282
BsrBI CCG'CTC
Cuts at: 0 864 1104
Size: 864 240
BsrDI GCAATG_nn'
Cuts at: 0 108 1104
Size: 108 996
BsrFI r'CCGG_y
Cuts at: 0 571 1104
Size: 571 533
BssHII G'CGCG_C
Cuts at: 0 390 500 535 702 896 898 1104
Size: 390 110 35 167 194 2 206
Fragments arranged by size:
390 206 194 167 110 35 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 381 1104
Size: 381 723
BtgI C'CryG_G
Cuts at: 0 50 167 350 587 641 650 1074 1104
Size: 50 117 183 237 54 9 424 30
Fragments arranged by size:
424 237 183 117 54 50 30 9
BtsI GCAGTG_nn'
Cuts at: 0 62 125 431 828 1104
Size: 62 63 306 397 276
Fragments arranged by size:
397 306 276 63 62
EaeI y'GGCC_r
Cuts at: 0 365 557 665 719 780 800 916 924
Size: 365 192 108 54 61 20 116 8
Cuts at: 924 1041 1104
Size: 117 63
Fragments arranged by size:
365 192 117 116 108 63 61 54
20 8
EagI C'GGCC_G
Cuts at: 0 665 780 924 1104
Size: 665 115 144 180
Fragments arranged by size:
665 180 144 115
EarI CTCTTCn'nnn_
Cuts at: 0 460 1104
Size: 460 644
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 693 895 958 1094 1104
Size: 693 202 63 136 10
Fragments arranged by size:
693 202 136 63 10
EcoICRI GAG'CTC
Cuts at: 0 510 1104
Size: 510 594
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 777 1104
Size: 526 251 327
Fragments arranged by size:
526 327 251
EcoO109I rG'GnC_Cy
Cuts at: 0 272 273 646 1047 1066 1104
Size: 272 1 373 401 19 38
Fragments arranged by size:
401 373 272 38 19 1
FspI TGC'GCA
Cuts at: 0 400 1104
Size: 400 704
HaeII r_GCGC'y
Cuts at: 0 454 466 1104
Size: 454 12 638
Fragments arranged by size:
638 454 12
KasI G'GCGC_C
Cuts at: 0 462 1104
Size: 462 642
KpnI G_GTAC'C
Cuts at: 0 363 1104
Size: 363 741
MscI TGG'CCA
Cuts at: 0 559 1104
Size: 559 545
MspA1I CmG'CkG
Cuts at: 0 381 652 1104
Size: 381 271 452
Fragments arranged by size:
452 381 271
NaeI GCC'GGC
Cuts at: 0 573 1104
Size: 573 531
NarI GG'CG_CC
Cuts at: 0 463 1104
Size: 463 641
NgoMIV G'CCGG_C
Cuts at: 0 571 1104
Size: 571 533
NotI GC'GGCC_GC
Cuts at: 0 924 1104
Size: 924 180
PasI CC'CwG_GG
Cuts at: 0 34 1058 1104
Size: 34 1024 46
Fragments arranged by size:
1024 46 34
PpuMI rG'GwC_Cy
Cuts at: 0 646 1066 1104
Size: 646 420 38
Fragments arranged by size:
646 420 38
PspOMI G'GGCC_C
Cuts at: 0 272 984 1047 1104
Size: 272 712 63 57
Fragments arranged by size:
712 272 63 57
PstI C_TGCA'G
Cuts at: 0 131 1104
Size: 131 973
SacI G_AGCT'C
Cuts at: 0 512 1104
Size: 512 592
SacII CC_GC'GG
Cuts at: 0 653 1104
Size: 653 451
SanDI GG'GwC_CC
Cuts at: 0 646 1066 1104
Size: 646 420 38
Fragments arranged by size:
646 420 38
SapI GCTCTTCn'nnn_
Cuts at: 0 460 1104
Size: 460 644
SfcI C'TryA_G
Cuts at: 0 127 1104
Size: 127 977
SfoI GGC'GCC
Cuts at: 0 464 1104
Size: 464 640
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 249 714 1104
Size: 249 465 390
Fragments arranged by size:
465 390 249
Enzymes that do cut:
AarI AccI Acc65I AcuI AlfI AlwNI ApaI ApaLI
AscI AvaI BanI BanII BbeI BbvCI BglI Bme1580I
BmgBI BpmI Bpu10I BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BstAPI BtgI BtsI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I FspI HaeII KasI KpnI MscI MspA1I
NaeI NarI NgoMIV NotI PasI PpuMI PspOMI PstI
SacI SacII SanDI SapI SfcI SfoI XcmI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApoI AseI AsiSI AvrII BaeI BamHI BbsI
BcgI BciVI BclI BfrBI BglII BlpI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BseRI BsgI BsiWI
BsmI BspEI BspHI BsrGI BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I
HincII HindIII HpaI MfeI MluI MmeI MslI NcoI
NdeI NheI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SalI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI