Restriction map for
pFN21AE9559
(Linear) (Six-base) MAPSORT of: KIEE9559.seq Check: 2544 from: 1 to: 1053
KIEE9559.orf
With 182 enzymes: SgfI *
August 25, 2011 14:40 ..
AleI CACnn'nnGTG
Cuts at: 0 392 1053
Size: 392 661
AvaI C'yCGr_G
Cuts at: 0 1018 1053
Size: 1018 35
BanI G'GyrC_C
Cuts at: 0 386 543 994 1053
Size: 386 157 451 59
Fragments arranged by size:
451 386 157 59
BanII G_rGCy'C
Cuts at: 0 71 582 1053
Size: 71 511 471
Fragments arranged by size:
511 471 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 591 1053
Size: 591 462
BglI GCCn_nnn'nGGC
Cuts at: 0 577 1053
Size: 577 476
BlpI GC'TnA_GC
Cuts at: 0 70 553 709 1053
Size: 70 483 156 344
Fragments arranged by size:
483 344 156 70
Bme1580I G_kGCm'C
Cuts at: 0 522 1053
Size: 522 531
BmrI ACTGGGnnnn_n'
Cuts at: 0 121 1053
Size: 121 932
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 90 876 1053
Size: 90 786 177
Fragments arranged by size:
786 177 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 1053
Size: 322 731
BsaI GGTCTCn'nnnn_
Cuts at: 0 25 694 1053
Size: 25 669 359
Fragments arranged by size:
669 359 25
BsaAI yAC'GTr
Cuts at: 0 642 1053
Size: 642 411
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 427 676 1053
Size: 397 30 249 377
Fragments arranged by size:
397 377 249 30
BsiHKAI G_wGCw'C
Cuts at: 0 71 1053
Size: 71 982
Bsp1286I G_dGCh'C
Cuts at: 0 71 522 582 1053
Size: 71 451 60 471
Fragments arranged by size:
471 451 71 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 1053
Size: 215 838
BsrBI CCG'CTC
Cuts at: 0 384 560 1053
Size: 384 176 493
Fragments arranged by size:
493 384 176
BsrDI GCAATG_nn'
Cuts at: 0 819 1053
Size: 819 234
BsrGI T'GTAC_A
Cuts at: 0 620 1053
Size: 620 433
BstAPI GCAn_nnn'nTGC
Cuts at: 0 327 1053
Size: 327 726
BstXI CCAn_nnnn'nTGG
Cuts at: 0 96 153 366 378 864 1053
Size: 96 57 213 12 486 189
Fragments arranged by size:
486 213 189 96 57 12
BtgI C'CryG_G
Cuts at: 0 224 377 429 1053
Size: 224 153 52 624
Fragments arranged by size:
624 224 153 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 153 357 1053
Size: 153 204 696
Fragments arranged by size:
696 204 153
BtsI GCAGTG_nn'
Cuts at: 0 344 361 399 507 1053
Size: 344 17 38 108 546
Fragments arranged by size:
546 344 108 38 17
EaeI y'GGCC_r
Cuts at: 0 221 356 368 401 432 569 848 1026
Size: 221 135 12 33 31 137 279 178
Cuts at: 1026 1053
Size: 27
Fragments arranged by size:
279 221 178 137 135 33 31 27
12
EarI CTCTTCn'nnn_
Cuts at: 0 241 262 1053
Size: 241 21 791
Fragments arranged by size:
791 241 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 907 934 1053
Size: 907 27 119
Fragments arranged by size:
907 119 27
EcoICRI GAG'CTC
Cuts at: 0 69 1053
Size: 69 984
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 1053
Size: 319 734
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 1053
Size: 517 32 504
Fragments arranged by size:
517 504 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 1053
Size: 200 853
MscI TGG'CCA
Cuts at: 0 358 370 1053
Size: 358 12 683
Fragments arranged by size:
683 358 12
MslI CAynn'nnrTG
Cuts at: 0 376 392 1053
Size: 376 16 661
Fragments arranged by size:
661 376 16
MspA1I CmG'CkG
Cuts at: 0 226 411 530 680 1053
Size: 226 185 119 150 373
Fragments arranged by size:
373 226 185 150 119
NspI r_CATG'y
Cuts at: 0 676 1053
Size: 676 377
PasI CC'CwG_GG
Cuts at: 0 474 987 1053
Size: 474 513 66
Fragments arranged by size:
513 474 66
PfoI T'CCnGG_A
Cuts at: 0 785 1053
Size: 785 268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 1053
Size: 295 32 726
Fragments arranged by size:
726 295 32
PshAI GACnn'nnGTC
Cuts at: 0 727 1053
Size: 727 326
PvuII CAG'CTG
Cuts at: 0 411 680 1053
Size: 411 269 373
Fragments arranged by size:
411 373 269
SacI G_AGCT'C
Cuts at: 0 71 1053
Size: 71 982
SacII CC_GC'GG
Cuts at: 0 227 1053
Size: 227 826
ScaI AGT'ACT
Cuts at: 0 835 1053
Size: 835 218
SfcI C'TryA_G
Cuts at: 0 824 1053
Size: 824 229
SmlI C'TyrA_G
Cuts at: 0 301 1053
Size: 301 752
StyI C'CwwG_G
Cuts at: 0 89 1053
Size: 89 964
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 453 1046 1053
Size: 453 593 7
Fragments arranged by size:
593 453 7
TatI w'GTAC_w
Cuts at: 0 620 833 881 1053
Size: 620 213 48 172
Fragments arranged by size:
620 213 172 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 961 993 1053
Size: 961 32 60
Fragments arranged by size:
961 60 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 711 1053
Size: 711 342
Enzymes that do cut:
AleI AvaI BanI BanII BbsI BglI BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrGI BstAPI BstXI BtgI BtgZI
BtsI EaeI EarI EciI EcoICRI EcoNI Hin4I MmeI
MscI MslI MspA1I NspI PasI PfoI PpiI PshAI
PvuII SacI SacII ScaI SfcI SmlI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
BpmI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BssHII
BssSI BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI Eco57MI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpuMI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI