Restriction map for
pFN21AE9552
(Linear) (Six-base) MAPSORT of: KIEE9552.seq Check: 8298 from: 1 to: 1164
KIEE9552.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 592 864 1164
Size: 592 272 300
Fragments arranged by size:
592 300 272
AatII G_ACGT'C
Cuts at: 0 302 1164
Size: 302 862
AfeI AGC'GCT
Cuts at: 0 997 1164
Size: 997 167
AflIII A'CryG_T
Cuts at: 0 1065 1164
Size: 1065 99
AseI AT'TA_AT
Cuts at: 0 418 1052 1164
Size: 418 634 112
Fragments arranged by size:
634 418 112
BamHI G'GATC_C
Cuts at: 0 843 1057 1164
Size: 843 214 107
Fragments arranged by size:
843 214 107
BanI G'GyrC_C
Cuts at: 0 229 816 1164
Size: 229 587 348
Fragments arranged by size:
587 348 229
BbsI GAAGACnn'nnnn_
Cuts at: 0 546 1011 1164
Size: 546 465 153
Fragments arranged by size:
546 465 153
BlpI GC'TnA_GC
Cuts at: 0 1148 1164
Size: 1148 16
Bme1580I G_kGCm'C
Cuts at: 0 819 1164
Size: 819 345
BmgBI CAC'GTC
Cuts at: 0 961 1164
Size: 961 203
BmrI ACTGGGnnnn_n'
Cuts at: 0 1148 1164
Size: 1148 16
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 734 1164
Size: 734 430
Bpu10I CC'TnA_GC
Cuts at: 0 148 656 1164
Size: 148 508 508
Fragments arranged by size:
508 508 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 754 1164
Size: 754 410
BsaAI yAC'GTr
Cuts at: 0 545 1164
Size: 545 619
BsaBI GATnn'nnATC
Cuts at: 0 97 1164
Size: 97 1067
BsaHI Gr'CG_yC
Cuts at: 0 299 1164
Size: 299 865
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1096 1126 1164
Size: 1096 30 38
Fragments arranged by size:
1096 38 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 607 693 1164
Size: 46 561 86 471
Fragments arranged by size:
561 471 86 46
BseYI C'CCAG_C
Cuts at: 0 134 423 434 812 1164
Size: 134 289 11 378 352
Fragments arranged by size:
378 352 289 134 11
Bsp1286I G_dGCh'C
Cuts at: 0 819 1164
Size: 819 345
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 119 592 864 1164
Size: 119 473 272 300
Fragments arranged by size:
473 300 272 119
BstXI CCAn_nnnn'nTGG
Cuts at: 0 240 840 1164
Size: 240 600 324
Fragments arranged by size:
600 324 240
BstYI r'GATC_y
Cuts at: 0 195 674 843 1057 1164
Size: 195 479 169 214 107
Fragments arranged by size:
479 214 195 169 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 396 730 1164
Size: 396 334 434
Fragments arranged by size:
434 396 334
BtsI GCAGTG_nn'
Cuts at: 0 1088 1164
Size: 1088 76
ClaI AT'CG_AT
Cuts at: 0 319 1164
Size: 319 845
DrdI GACnn_nn'nnGTC
Cuts at: 0 862 1164
Size: 862 302
EaeI y'GGCC_r
Cuts at: 0 95 1095 1164
Size: 95 1000 69
Fragments arranged by size:
1000 95 69
FspI TGC'GCA
Cuts at: 0 409 1164
Size: 409 755
HaeII r_GCGC'y
Cuts at: 0 999 1164
Size: 999 165
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 387 419 1098 1130 1164
Size: 387 32 679 32 34
Fragments arranged by size:
679 387 34 32 32
HincII GTy'rAC
Cuts at: 0 564 1164
Size: 564 600
MscI TGG'CCA
Cuts at: 0 97 1097 1164
Size: 97 1000 67
Fragments arranged by size:
1000 97 67
MslI CAynn'nnrTG
Cuts at: 0 790 1164
Size: 790 374
NspI r_CATG'y
Cuts at: 0 667 1069 1164
Size: 667 402 95
Fragments arranged by size:
667 402 95
PasI CC'CwG_GG
Cuts at: 0 868 1164
Size: 868 296
PciI A'CATG_T
Cuts at: 0 1065 1164
Size: 1065 99
PflMI CCAn_nnn'nTGG
Cuts at: 0 378 1164
Size: 378 786
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 1164
Size: 523 32 609
Fragments arranged by size:
609 523 32
SfcI C'TryA_G
Cuts at: 0 384 1164
Size: 384 780
SnaBI TAC'GTA
Cuts at: 0 545 1164
Size: 545 619
SphI G_CATG'C
Cuts at: 0 667 1164
Size: 667 497
SspI AAT'ATT
Cuts at: 0 837 1164
Size: 837 327
StuI AGG'CCT
Cuts at: 0 717 1164
Size: 717 447
TatI w'GTAC_w
Cuts at: 0 1068 1164
Size: 1068 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1096 1128 1164
Size: 1096 32 36
Fragments arranged by size:
1096 36 32
XmnI GAAnn'nnTTC
Cuts at: 0 1008 1164
Size: 1008 156
ZraI GAC'GTC
Cuts at: 0 300 1164
Size: 300 864
Enzymes that do cut:
AarI AatII AfeI AflIII AseI BamHI BanI BbsI
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI Bsp1286I BspMI BstXI
BstYI BtgZI BtsI ClaI DrdI EaeI FspI HaeII
Hin4I HincII MscI MslI NspI PasI PciI PflMI
PpiI SfcI SnaBI SphI SspI StuI TatI TstI
XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AsiSI
AvaI AvrII BaeI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmtI BpmI BpuEI BsaWI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI CspCI DraI DraIII EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PfoI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SrfI StyI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI