Restriction map for
pFN21AE9550
(Linear) (Six-base) MAPSORT of: KIEE9550.seq Check: 9479 from: 1 to: 1194
KIEE9550.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 667 732 1194
Size: 667 65 462
Fragments arranged by size:
667 462 65
AflII C'TTAA_G
Cuts at: 0 910 1194
Size: 910 284
AflIII A'CryG_T
Cuts at: 0 29 385 1194
Size: 29 356 809
Fragments arranged by size:
809 356 29
AleI CACnn'nnGTG
Cuts at: 0 262 1194
Size: 262 932
AlwNI CAG_nnn'CTG
Cuts at: 0 497 747 861 1194
Size: 497 250 114 333
Fragments arranged by size:
497 333 250 114
ApaI G_GGCC'C
Cuts at: 0 832 868 1194
Size: 832 36 326
Fragments arranged by size:
832 326 36
AvaI C'yCGr_G
Cuts at: 0 66 553 1194
Size: 66 487 641
Fragments arranged by size:
641 487 66
BanI G'GyrC_C
Cuts at: 0 42 76 763 881 1194
Size: 42 34 687 118 313
Fragments arranged by size:
687 313 118 42 34
BanII G_rGCy'C
Cuts at: 0 832 868 877 924 982 1194
Size: 832 36 9 47 58 212
Fragments arranged by size:
832 212 58 47 36 9
BbeI G_GCGC'C
Cuts at: 0 80 767 1194
Size: 80 687 427
Fragments arranged by size:
687 427 80
BbvCI CC'TCA_GC
Cuts at: 0 490 1194
Size: 490 704
BglI GCCn_nnn'nGGC
Cuts at: 0 250 692 772 872 1194
Size: 250 442 80 100 322
Fragments arranged by size:
442 322 250 100 80
Bme1580I G_kGCm'C
Cuts at: 0 45 832 868 1194
Size: 45 787 36 326
Fragments arranged by size:
787 326 45 36
BmgBI CAC'GTC
Cuts at: 0 264 1194
Size: 264 930
BmrI ACTGGGnnnn_n'
Cuts at: 0 91 1194
Size: 91 1103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1194
Size: 619 575
Bpu10I CC'TnA_GC
Cuts at: 0 490 1194
Size: 490 704
BsaI GGTCTCn'nnnn_
Cuts at: 0 375 786 1194
Size: 375 411 408
Fragments arranged by size:
411 408 375
BsaAI yAC'GTr
Cuts at: 0 386 426 1194
Size: 386 40 768
Fragments arranged by size:
768 386 40
BsaHI Gr'CG_yC
Cuts at: 0 77 122 764 1194
Size: 77 45 642 430
Fragments arranged by size:
642 430 77 45
BsaWI w'CCGG_w
Cuts at: 0 19 1194
Size: 19 1175
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 928 958 1194
Size: 928 30 236
Fragments arranged by size:
928 236 30
BseYI C'CCAG_C
Cuts at: 0 162 320 327 586 824 861 1194
Size: 162 158 7 259 238 37 333
Fragments arranged by size:
333 259 238 162 158 37 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 436 523 1072 1194
Size: 397 39 87 549 122
Fragments arranged by size:
549 397 122 87 39
BsiEI CG_ry'CG
Cuts at: 0 800 1194
Size: 800 394
BsiHKAI G_wGCw'C
Cuts at: 0 533 1194
Size: 533 661
Bsp1286I G_dGCh'C
Cuts at: 0 45 533 832 868 877 924 982 1194
Size: 45 488 299 36 9 47 58 212
Fragments arranged by size:
488 299 212 58 47 45 36 9
BspEI T'CCGG_A
Cuts at: 0 19 1194
Size: 19 1175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 357 632 647 667 732 1194
Size: 357 275 15 20 65 462
Fragments arranged by size:
462 357 275 65 20 15
BsrBI CCG'CTC
Cuts at: 0 138 1194
Size: 138 1056
BsrFI r'CCGG_y
Cuts at: 0 45 525 1046 1194
Size: 45 480 521 148
Fragments arranged by size:
521 480 148 45
BsrGI T'GTAC_A
Cuts at: 0 617 1194
Size: 617 577
BssHII G'CGCG_C
Cuts at: 0 444 561 603 756 1019 1153 1194
Size: 444 117 42 153 263 134 41
Fragments arranged by size:
444 263 153 134 117 42 41
BssSI C'ACGA_G
Cuts at: 0 130 1194
Size: 130 1064
BstXI CCAn_nnnn'nTGG
Cuts at: 0 411 1194
Size: 411 783
BtgI C'CryG_G
Cuts at: 0 8 109 116 257 800 869 1194
Size: 8 101 7 141 543 69 325
Fragments arranged by size:
543 325 141 101 69 8 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 225 1194
Size: 225 969
BtsI GCAGTG_nn'
Cuts at: 0 579 924 1146 1194
Size: 579 345 222 48
Fragments arranged by size:
579 345 222 48
DraIII CAC_nnn'GTG
Cuts at: 0 9 1194
Size: 9 1185
DrdI GACnn_nn'nnGTC
Cuts at: 0 97 1194
Size: 97 1097
EaeI y'GGCC_r
Cuts at: 0 47 254 674 797 1148 1194
Size: 47 207 420 123 351 46
Fragments arranged by size:
420 351 207 123 47 46
EagI C'GGCC_G
Cuts at: 0 797 1194
Size: 797 397
EarI CTCTTCn'nnn_
Cuts at: 0 145 817 1194
Size: 145 672 377
Fragments arranged by size:
672 377 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 991 1194
Size: 991 203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1194
Size: 619 575
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1174 1194
Size: 1174 20
EcoO109I rG'GnC_Cy
Cuts at: 0 828 829 865 1100 1194
Size: 828 1 36 235 94
Fragments arranged by size:
828 235 94 36 1
HaeII r_GCGC'y
Cuts at: 0 80 154 478 634 731 767 862 1194
Size: 80 74 324 156 97 36 95 332
Fragments arranged by size:
332 324 156 97 95 80 74 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 906 938 1194
Size: 906 32 256
Fragments arranged by size:
906 256 32
HincII GTy'rAC
Cuts at: 0 354 1194
Size: 354 840
KasI G'GCGC_C
Cuts at: 0 76 763 1194
Size: 76 687 431
Fragments arranged by size:
687 431 76
MluI A'CGCG_T
Cuts at: 0 29 1194
Size: 29 1165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1014 1194
Size: 1014 180
MslI CAynn'nnrTG
Cuts at: 0 262 409 1194
Size: 262 147 785
Fragments arranged by size:
785 262 147
MspA1I CmG'CkG
Cuts at: 0 111 312 419 648 840 871 960 1194
Size: 111 201 107 229 192 31 89 234
Fragments arranged by size:
234 229 201 192 111 107 89 31
NaeI GCC'GGC
Cuts at: 0 1048 1194
Size: 1048 146
NarI GG'CG_CC
Cuts at: 0 77 764 1194
Size: 77 687 430
Fragments arranged by size:
687 430 77
NgoMIV G'CCGG_C
Cuts at: 0 1046 1194
Size: 1046 148
NspI r_CATG'y
Cuts at: 0 933 1194
Size: 933 261
PmlI CAC'GTG
Cuts at: 0 386 1194
Size: 386 808
PpuMI rG'GwC_Cy
Cuts at: 0 1100 1194
Size: 1100 94
PspOMI G'GGCC_C
Cuts at: 0 828 864 1194
Size: 828 36 330
Fragments arranged by size:
828 330 36
PstI C_TGCA'G
Cuts at: 0 574 737 857 1194
Size: 574 163 120 337
Fragments arranged by size:
574 337 163 120
PvuII CAG'CTG
Cuts at: 0 840 1194
Size: 840 354
RsrII CG'GwC_CG
Cuts at: 0 221 1194
Size: 221 973
SacII CC_GC'GG
Cuts at: 0 112 872 1194
Size: 112 760 322
Fragments arranged by size:
760 322 112
SanDI GG'GwC_CC
Cuts at: 0 1100 1194
Size: 1100 94
SapI GCTCTTCn'nnn_
Cuts at: 0 145 817 1194
Size: 145 672 377
Fragments arranged by size:
672 377 145
SbfI CC_TGCA'GG
Cuts at: 0 737 1194
Size: 737 457
SfcI C'TryA_G
Cuts at: 0 570 733 853 936 1194
Size: 570 163 120 83 258
Fragments arranged by size:
570 258 163 120 83
SfoI GGC'GCC
Cuts at: 0 78 765 1194
Size: 78 687 429
Fragments arranged by size:
687 429 78
SgrAI Cr'CCGG_yG
Cuts at: 0 525 1194
Size: 525 669
SmaI CCC'GGG
Cuts at: 0 68 555 1194
Size: 68 487 639
Fragments arranged by size:
639 487 68
SmlI C'TyrA_G
Cuts at: 0 910 1194
Size: 910 284
SrfI GCCC'GGGC
Cuts at: 0 68 1194
Size: 68 1126
StuI AGG'CCT
Cuts at: 0 580 997 1194
Size: 580 417 197
Fragments arranged by size:
580 417 197
StyI C'CwwG_G
Cuts at: 0 776 1194
Size: 776 418
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 312 899 1194
Size: 312 587 295
Fragments arranged by size:
587 312 295
TatI w'GTAC_w
Cuts at: 0 617 1194
Size: 617 577
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 916 948 1194
Size: 916 32 246
Fragments arranged by size:
916 246 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 594 1194
Size: 594 600
XmaI C'CCGG_G
Cuts at: 0 66 553 1194
Size: 66 487 641
Fragments arranged by size:
641 487 66
Enzymes that do cut:
AarI AflII AflIII AleI AlwNI ApaI AvaI BanI
BanII BbeI BbvCI BglI Bme1580I BmgBI BmrI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstXI BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII KasI MluI MmeI MslI MspA1I NaeI
NarI NgoMIV NspI PmlI PpuMI PspOMI PstI PvuII
RsrII SacII SanDI SapI SbfI SfcI SfoI SgrAI
SmaI SmlI SrfI StuI StyI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BplI BpuEI BsaBI BseRI BsiWI BsmI
BsmBI BspHI BsrDI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I ClaI CspCI DraI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MscI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI SacI SalI ScaI SexAI SfiI SgfI
SnaBI SpeI SphI SspI SwaI Tth111I XbaI XhoI
XmnI ZraI