Restriction map for
pFN21AE9546
(Linear) (Six-base) MAPSORT of: KIEE9546.seq Check: 2973 from: 1 to: 1566
KIEE9546.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AatII G_ACGT'C
Cuts at: 0 56 177 1566
Size: 56 121 1389
Fragments arranged by size:
1389 121 56
AccI GT'mk_AC
Cuts at: 0 749 914 1566
Size: 749 165 652
Fragments arranged by size:
749 652 165
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 683 1029 1566
Size: 317 366 346 537
Fragments arranged by size:
537 366 346 317
AflIII A'CryG_T
Cuts at: 0 852 1566
Size: 852 714
AleI CACnn'nnGTG
Cuts at: 0 967 1566
Size: 967 599
AlwNI CAG_nnn'CTG
Cuts at: 0 236 1566
Size: 236 1330
ApoI r'AATT_y
Cuts at: 0 1034 1320 1566
Size: 1034 286 246
Fragments arranged by size:
1034 286 246
AseI AT'TA_AT
Cuts at: 0 395 1566
Size: 395 1171
BanI G'GyrC_C
Cuts at: 0 35 920 1566
Size: 35 885 646
Fragments arranged by size:
885 646 35
BanII G_rGCy'C
Cuts at: 0 288 1566
Size: 288 1278
BbsI GAAGACnn'nnnn_
Cuts at: 0 935 1566
Size: 935 631
BclI T'GATC_A
Cuts at: 0 800 1566
Size: 800 766
BglI GCCn_nnn'nGGC
Cuts at: 0 283 1566
Size: 283 1283
BglII A'GATC_T
Cuts at: 0 956 1566
Size: 956 610
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 901 1566
Size: 901 665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 163 234 1339 1566
Size: 150 13 71 1105 227
Fragments arranged by size:
1105 227 150 71 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 1566
Size: 416 1150
BsaAI yAC'GTr
Cuts at: 0 1095 1566
Size: 1095 471
BsaHI Gr'CG_yC
Cuts at: 0 53 174 1566
Size: 53 121 1392
Fragments arranged by size:
1392 121 53
BsaWI w'CCGG_w
Cuts at: 0 66 1566
Size: 66 1500
BseYI C'CCAG_C
Cuts at: 0 21 180 1566
Size: 21 159 1386
Fragments arranged by size:
1386 159 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 1566
Size: 47 1519
BsiHKAI G_wGCw'C
Cuts at: 0 99 1421 1566
Size: 99 1322 145
Fragments arranged by size:
1322 145 99
BsmI GAATG_Cn'
Cuts at: 0 1270 1566
Size: 1270 296
BsmBI CGTCTCn'nnnn_
Cuts at: 0 60 1566
Size: 60 1506
Bsp1286I G_dGCh'C
Cuts at: 0 99 288 1421 1566
Size: 99 189 1133 145
Fragments arranged by size:
1133 189 145 99
BspHI T'CATG_A
Cuts at: 0 305 1566
Size: 305 1261
BsrBI CCG'CTC
Cuts at: 0 134 1566
Size: 134 1432
BsrFI r'CCGG_y
Cuts at: 0 191 1566
Size: 191 1375
BstAPI GCAn_nnn'nTGC
Cuts at: 0 596 1566
Size: 596 970
BstXI CCAn_nnnn'nTGG
Cuts at: 0 917 1566
Size: 917 649
BstYI r'GATC_y
Cuts at: 0 956 1566
Size: 956 610
BtgI C'CryG_G
Cuts at: 0 161 1329 1566
Size: 161 1168 237
Fragments arranged by size:
1168 237 161
BtsI GCAGTG_nn'
Cuts at: 0 610 1093 1290 1563 1566
Size: 610 483 197 273 3
Fragments arranged by size:
610 483 273 197 3
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 648 683 1566
Size: 648 35 883
Fragments arranged by size:
883 648 35
DraI TTT'AAA
Cuts at: 0 759 1566
Size: 759 807
DraIII CAC_nnn'GTG
Cuts at: 0 656 1098 1566
Size: 656 442 468
Fragments arranged by size:
656 468 442
EaeI y'GGCC_r
Cuts at: 0 26 566 637 934 1566
Size: 26 540 71 297 632
Fragments arranged by size:
632 540 297 71 26
EarI CTCTTCn'nnn_
Cuts at: 0 336 556 1048 1566
Size: 336 220 492 518
Fragments arranged by size:
518 492 336 220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1315 1566
Size: 1315 251
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 163 234 317 683 1029 1339 1566
Size: 150 13 71 83 366 346 310 227
Fragments arranged by size:
366 346 310 227 150 83 71 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1064 1566
Size: 1064 502
EcoO109I rG'GnC_Cy
Cuts at: 0 810 1566
Size: 810 756
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 313 345 1566
Size: 43 32 238 32 1221
Fragments arranged by size:
1221 238 43 32 32
HindIII A'AGCT_T
Cuts at: 0 1343 1388 1566
Size: 1343 45 178
Fragments arranged by size:
1343 178 45
MfeI C'AATT_G
Cuts at: 0 719 1566
Size: 719 847
MscI TGG'CCA
Cuts at: 0 568 639 936 1566
Size: 568 71 297 630
Fragments arranged by size:
630 568 297 71
MslI CAynn'nnrTG
Cuts at: 0 796 826 967 1126 1566
Size: 796 30 141 159 440
Fragments arranged by size:
796 440 159 141 30
MspA1I CmG'CkG
Cuts at: 0 25 32 163 1566
Size: 25 7 131 1403
Fragments arranged by size:
1403 131 25 7
NaeI GCC'GGC
Cuts at: 0 193 1566
Size: 193 1373
NdeI CA'TA_TG
Cuts at: 0 530 1566
Size: 530 1036
NgoMIV G'CCGG_C
Cuts at: 0 191 1566
Size: 191 1375
NspI r_CATG'y
Cuts at: 0 603 856 1566
Size: 603 253 710
Fragments arranged by size:
710 603 253
PciI A'CATG_T
Cuts at: 0 852 1566
Size: 852 714
PflMI CCAn_nnn'nTGG
Cuts at: 0 744 1566
Size: 744 822
PfoI T'CCnGG_A
Cuts at: 0 41 1102 1445 1566
Size: 41 1061 343 121
Fragments arranged by size:
1061 343 121 41
PmlI CAC'GTG
Cuts at: 0 1095 1566
Size: 1095 471
PpuMI rG'GwC_Cy
Cuts at: 0 810 1566
Size: 810 756
PshAI GACnn'nnGTC
Cuts at: 0 173 1566
Size: 173 1393
PstI C_TGCA'G
Cuts at: 0 247 843 1056 1229 1566
Size: 247 596 213 173 337
Fragments arranged by size:
596 337 247 213 173
PvuII CAG'CTG
Cuts at: 0 32 1566
Size: 32 1534
SacII CC_GC'GG
Cuts at: 0 164 1566
Size: 164 1402
SfcI C'TryA_G
Cuts at: 0 243 725 839 1052 1225 1566
Size: 243 482 114 213 173 341
Fragments arranged by size:
482 341 243 213 173 114
SmlI C'TyrA_G
Cuts at: 0 431 1566
Size: 431 1135
SpeI A'CTAG_T
Cuts at: 0 582 1566
Size: 582 984
SphI G_CATG'C
Cuts at: 0 603 1566
Size: 603 963
SspI AAT'ATT
Cuts at: 0 399 624 871 1566
Size: 399 225 247 695
Fragments arranged by size:
695 399 247 225
TatI w'GTAC_w
Cuts at: 0 383 1566
Size: 383 1183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 89 121 482 514 1084 1116 1566
Size: 89 32 361 32 570 32 450
Fragments arranged by size:
570 450 361 89 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 29 987 1566
Size: 29 958 579
Fragments arranged by size:
958 579 29
XmnI GAAnn'nnTTC
Cuts at: 0 7 1566
Size: 7 1559
ZraI GAC'GTC
Cuts at: 0 54 175 1566
Size: 54 121 1391
Fragments arranged by size:
1391 121 54
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AlwNI ApoI AseI
BanI BanII BbsI BclI BglI BglII BplI BpmI
BpuEI BsaAI BsaHI BsaWI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BsrBI BsrFI BstAPI BstXI BstYI
BtgI BtsI CspCI DraI DraIII EaeI EarI EciI
Eco57MI EcoNI EcoO109I Hin4I HindIII MfeI MscI MslI
MspA1I NaeI NdeI NgoMIV NspI PciI PflMI PfoI
PmlI PpuMI PshAI PstI PvuII SacII SfcI SmlI
SpeI SphI SspI TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BfrBI BlpI Bme1580I
BmgBI BmrI BmtI Bpu10I BsaI BsaBI BsaXI BseRI
BsiEI BsiWI BspEI BspMI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DrdI EagI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MluI MmeI NarI NcoI
NheI NotI NruI NsiI PacI PasI PmeI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SrfI StuI StyI SwaI TaqII
Tth111I XbaI XhoI XmaI