Restriction map for
pFN21AE9544
(Linear) (Six-base) MAPSORT of: KIEE9544.seq Check: 3993 from: 1 to: 1398
KIEE9544.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
AccI GT'mk_AC
Cuts at: 0 716 1398
Size: 716 682
Acc65I G'GTAC_C
Cuts at: 0 122 257 833 1398
Size: 122 135 576 565
Fragments arranged by size:
576 565 135 122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 1398
Size: 152 1246
AfeI AGC'GCT
Cuts at: 0 112 1398
Size: 112 1286
AflIII A'CryG_T
Cuts at: 0 692 1398
Size: 692 706
AlwNI CAG_nnn'CTG
Cuts at: 0 27 1360 1398
Size: 27 1333 38
Fragments arranged by size:
1333 38 27
ApaI G_GGCC'C
Cuts at: 0 798 1290 1398
Size: 798 492 108
Fragments arranged by size:
798 492 108
AseI AT'TA_AT
Cuts at: 0 926 1398
Size: 926 472
AvaI C'yCGr_G
Cuts at: 0 1094 1308 1341 1398
Size: 1094 214 33 57
Fragments arranged by size:
1094 214 57 33
BanI G'GyrC_C
Cuts at: 0 122 257 269 682 833 1038 1099 1220
Size: 122 135 12 413 151 205 61 121
Cuts at: 1220 1398
Size: 178
Fragments arranged by size:
413 205 178 151 135 122 121 61
12
BanII G_rGCy'C
Cuts at: 0 798 1290 1398
Size: 798 492 108
Fragments arranged by size:
798 492 108
BbeI G_GCGC'C
Cuts at: 0 1103 1398
Size: 1103 295
BbvCI CC'TCA_GC
Cuts at: 0 973 1009 1138 1398
Size: 973 36 129 260
Fragments arranged by size:
973 260 129 36
BglI GCCn_nnn'nGGC
Cuts at: 0 308 1285 1398
Size: 308 977 113
Fragments arranged by size:
977 308 113
BglII A'GATC_T
Cuts at: 0 1130 1398
Size: 1130 268
Bme1580I G_kGCm'C
Cuts at: 0 798 1043 1290 1398
Size: 798 245 247 108
Fragments arranged by size:
798 247 245 108
BmgBI CAC'GTC
Cuts at: 0 533 1398
Size: 533 865
BmrI ACTGGGnnnn_n'
Cuts at: 0 663 1398
Size: 663 735
BmtI G_CTAG'C
Cuts at: 0 463 1398
Size: 463 935
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 93 1034 1398
Size: 93 941 364
Fragments arranged by size:
941 364 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1398
Size: 43 1355
Bpu10I CC'TnA_GC
Cuts at: 0 973 1009 1138 1398
Size: 973 36 129 260
Fragments arranged by size:
973 260 129 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 1398
Size: 495 903
BsaI GGTCTCn'nnnn_
Cuts at: 0 136 332 563 1398
Size: 136 196 231 835
Fragments arranged by size:
835 231 196 136
BsaAI yAC'GTr
Cuts at: 0 695 1398
Size: 695 703
BsaHI Gr'CG_yC
Cuts at: 0 713 1100 1398
Size: 713 387 298
Fragments arranged by size:
713 387 298
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 24 1183 1213 1250 1280 1398
Size: 24 1159 30 37 30 118
Fragments arranged by size:
1159 118 37 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 769 772 1267 1398
Size: 769 3 495 131
Fragments arranged by size:
769 495 131 3
BseYI C'CCAG_C
Cuts at: 0 113 209 788 857 1047 1398
Size: 113 96 579 69 190 351
Fragments arranged by size:
579 351 190 113 96 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 706 1216 1398
Size: 206 500 510 182
Fragments arranged by size:
510 500 206 182
BsiHKAI G_wGCw'C
Cuts at: 0 740 1398
Size: 740 658
BsmI GAATG_Cn'
Cuts at: 0 947 956 1398
Size: 947 9 442
Fragments arranged by size:
947 442 9
Bsp1286I G_dGCh'C
Cuts at: 0 740 798 1043 1290 1398
Size: 740 58 245 247 108
Fragments arranged by size:
740 247 245 108 58
BspHI T'CATG_A
Cuts at: 0 911 1398
Size: 911 487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 269 277 402 728 785 1398
Size: 269 8 125 326 57 613
Fragments arranged by size:
613 326 269 125 57 8
BsrBI CCG'CTC
Cuts at: 0 405 1398
Size: 405 993
BsrDI GCAATG_nn'
Cuts at: 0 495 1398
Size: 495 903
BsrFI r'CCGG_y
Cuts at: 0 1004 1398
Size: 1004 394
BsrGI T'GTAC_A
Cuts at: 0 419 1398
Size: 419 979
BssHII G'CGCG_C
Cuts at: 0 1275 1398
Size: 1275 123
BssSI C'ACGA_G
Cuts at: 0 739 1057 1398
Size: 739 318 341
Fragments arranged by size:
739 341 318
BstAPI GCAn_nnn'nTGC
Cuts at: 0 27 134 229 1398
Size: 27 107 95 1169
Fragments arranged by size:
1169 107 95 27
BstYI r'GATC_y
Cuts at: 0 1130 1205 1398
Size: 1130 75 193
Fragments arranged by size:
1130 193 75
BtgI C'CryG_G
Cuts at: 0 155 1212 1329 1398
Size: 155 1057 117 69
Fragments arranged by size:
1057 155 117 69
BtsI GCAGTG_nn'
Cuts at: 0 233 318 451 859 1224 1398
Size: 233 85 133 408 365 174
Fragments arranged by size:
408 365 233 174 133 85
DraIII CAC_nnn'GTG
Cuts at: 0 279 352 854 1062 1398
Size: 279 73 502 208 336
Fragments arranged by size:
502 336 279 208 73
EaeI y'GGCC_r
Cuts at: 0 318 554 1398
Size: 318 236 844
Fragments arranged by size:
844 318 236
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 82 289 1198 1398
Size: 82 207 909 200
Fragments arranged by size:
909 207 200 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 152 1398
Size: 43 109 1246
Fragments arranged by size:
1246 109 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1249 1398
Size: 1249 149
EcoO109I rG'GnC_Cy
Cuts at: 0 188 612 633 794 795 1287 1317 1346
Size: 188 424 21 161 1 492 30 29
Cuts at: 1346 1357 1398
Size: 11 41
Fragments arranged by size:
492 424 188 161 41 30 29 21
11 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1140 1398
Size: 1140 258
HaeII r_GCGC'y
Cuts at: 0 114 1103 1398
Size: 114 989 295
Fragments arranged by size:
989 295 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 1183 1215 1398
Size: 26 1157 32 183
Fragments arranged by size:
1157 183 32 26
KasI G'GCGC_C
Cuts at: 0 1099 1398
Size: 1099 299
KpnI G_GTAC'C
Cuts at: 0 126 261 837 1398
Size: 126 135 576 561
Fragments arranged by size:
576 561 135 126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1181 1364 1398
Size: 1181 183 34
Fragments arranged by size:
1181 183 34
MscI TGG'CCA
Cuts at: 0 320 1398
Size: 320 1078
MslI CAynn'nnrTG
Cuts at: 0 275 910 1398
Size: 275 635 488
Fragments arranged by size:
635 488 275
MspA1I CmG'CkG
Cuts at: 0 24 157 1331 1398
Size: 24 133 1174 67
Fragments arranged by size:
1174 133 67 24
NarI GG'CG_CC
Cuts at: 0 1100 1398
Size: 1100 298
NheI G'CTAG_C
Cuts at: 0 459 1398
Size: 459 939
PasI CC'CwG_GG
Cuts at: 0 818 1354 1398
Size: 818 536 44
Fragments arranged by size:
818 536 44
PflMI CCAn_nnn'nTGG
Cuts at: 0 279 887 1062 1360 1398
Size: 279 608 175 298 38
Fragments arranged by size:
608 298 279 175 38
PfoI T'CCnGG_A
Cuts at: 0 1268 1398
Size: 1268 130
PmlI CAC'GTG
Cuts at: 0 695 1398
Size: 695 703
PpuMI rG'GwC_Cy
Cuts at: 0 188 1317 1346 1398
Size: 188 1129 29 52
Fragments arranged by size:
1129 188 52 29
PspOMI G'GGCC_C
Cuts at: 0 794 1286 1398
Size: 794 492 112
Fragments arranged by size:
794 492 112
PstI C_TGCA'G
Cuts at: 0 23 288 1398
Size: 23 265 1110
Fragments arranged by size:
1110 265 23
PvuII CAG'CTG
Cuts at: 0 24 1398
Size: 24 1374
SacII CC_GC'GG
Cuts at: 0 158 1332 1398
Size: 158 1174 66
Fragments arranged by size:
1174 158 66
SanDI GG'GwC_CC
Cuts at: 0 1346 1398
Size: 1346 52
ScaI AGT'ACT
Cuts at: 0 673 1398
Size: 673 725
SfcI C'TryA_G
Cuts at: 0 19 284 1398
Size: 19 265 1114
Fragments arranged by size:
1114 265 19
SfoI GGC'GCC
Cuts at: 0 1101 1398
Size: 1101 297
SmaI CCC'GGG
Cuts at: 0 1096 1310 1398
Size: 1096 214 88
Fragments arranged by size:
1096 214 88
SmlI C'TyrA_G
Cuts at: 0 474 1398
Size: 474 924
SrfI GCCC'GGGC
Cuts at: 0 1310 1398
Size: 1310 88
StuI AGG'CCT
Cuts at: 0 173 376 1398
Size: 173 203 1022
Fragments arranged by size:
1022 203 173
StyI C'CwwG_G
Cuts at: 0 146 600 1398
Size: 146 454 798
Fragments arranged by size:
798 454 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 240 670 810 1039 1398
Size: 240 430 140 229 359
Fragments arranged by size:
430 359 240 229 140
TatI w'GTAC_w
Cuts at: 0 419 671 1398
Size: 419 252 727
Fragments arranged by size:
727 419 252
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1032 1380 1398
Size: 1032 348 18
Fragments arranged by size:
1032 348 18
XmaI C'CCGG_G
Cuts at: 0 1094 1308 1398
Size: 1094 214 90
Fragments arranged by size:
1094 214 90
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AlwNI ApaI AseI
AvaI BanI BanII BbeI BbvCI BglI BglII Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstYI BtgI BtsI DraIII EaeI EciI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI KpnI
MmeI MscI MslI MspA1I NarI NheI PasI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SacII SanDI
ScaI SfcI SfoI SmaI SmlI SrfI StuI StyI
TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AscI AsiSI AvrII BaeI BamHI
BbsI BcgI BciVI BclI BfrBI BlpI BsaBI BsaWI
BsiEI BsiWI BsmBI BspEI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI
EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI MfeI MluI NaeI NcoI NdeI NgoMIV NotI
NruI NsiI NspI PacI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SapI
SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI TstI Tth111I XbaI XhoI XmnI ZraI