Restriction map for
pFN21AE9542
(Linear) (Six-base) MAPSORT of: KIEE9542.seq Check: 1984 from: 1 to: 2271
KIEE9542.orf
With 182 enzymes: SgfI *
March 22, 2012 18:30 ..
Acc65I G'GTAC_C
Cuts at: 0 1975 2271
Size: 1975 296
AclI AA'CG_TT
Cuts at: 0 1884 2271
Size: 1884 387
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 439 586 973 1942 2271
Size: 382 57 147 387 969 329
Fragments arranged by size:
969 387 382 329 147 57
AflIII A'CryG_T
Cuts at: 0 1152 2271
Size: 1152 1119
AhdI GACnn_n'nnGTC
Cuts at: 0 70 2271
Size: 70 2201
AleI CACnn'nnGTG
Cuts at: 0 399 2271
Size: 399 1872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1272 2271
Size: 1272 999
AlwNI CAG_nnn'CTG
Cuts at: 0 132 462 888 1840 2271
Size: 132 330 426 952 431
Fragments arranged by size:
952 431 426 330 132
ApoI r'AATT_y
Cuts at: 0 635 663 789 998 1061 1126 1184 1651
Size: 635 28 126 209 63 65 58 467
Cuts at: 1651 1942 2271
Size: 291 329
Fragments arranged by size:
635 467 329 291 209 126 65 63
58 28
AseI AT'TA_AT
Cuts at: 0 848 1760 2271
Size: 848 912 511
Fragments arranged by size:
912 848 511
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1475 1508 2271
Size: 1475 33 763
Fragments arranged by size:
1475 763 33
BanI G'GyrC_C
Cuts at: 0 1672 1975 2271
Size: 1672 303 296
Fragments arranged by size:
1672 303 296
BanII G_rGCy'C
Cuts at: 0 1728 2271
Size: 1728 543
BbsI GAAGACnn'nnnn_
Cuts at: 0 9 2271
Size: 9 2262
BbvCI CC'TCA_GC
Cuts at: 0 125 2271
Size: 125 2146
BciVI GTATCCnnnnn_n'
Cuts at: 0 1916 2271
Size: 1916 355
BfrBI ATG'CAT
Cuts at: 0 1912 2271
Size: 1912 359
BglII A'GATC_T
Cuts at: 0 585 2271
Size: 585 1686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 865 2271
Size: 346 519 1406
Fragments arranged by size:
1406 519 346
Bpu10I CC'TnA_GC
Cuts at: 0 125 2271
Size: 125 2146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 2271
Size: 1018 1253
BsaI GGTCTCn'nnnn_
Cuts at: 0 209 1557 2271
Size: 209 1348 714
Fragments arranged by size:
1348 714 209
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2124 2154 2271
Size: 2124 30 117
Fragments arranged by size:
2124 117 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 2271
Size: 118 2153
BsiHKAI G_wGCw'C
Cuts at: 0 82 2271
Size: 82 2189
Bsp1286I G_dGCh'C
Cuts at: 0 82 1728 2271
Size: 82 1646 543
Fragments arranged by size:
1646 543 82
BspHI T'CATG_A
Cuts at: 0 447 2271
Size: 447 1824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 476 1264 2271
Size: 476 788 1007
Fragments arranged by size:
1007 788 476
BsrDI GCAATG_nn'
Cuts at: 0 313 395 2271
Size: 313 82 1876
Fragments arranged by size:
1876 313 82
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2181 2271
Size: 2181 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1138 2271
Size: 1138 1133
BstYI r'GATC_y
Cuts at: 0 585 1465 2271
Size: 585 880 806
Fragments arranged by size:
880 806 585
BtsI GCAGTG_nn'
Cuts at: 0 1951 2170 2271
Size: 1951 219 101
Fragments arranged by size:
1951 219 101
DraI TTT'AAA
Cuts at: 0 2064 2271
Size: 2064 207
DraIII CAC_nnn'GTG
Cuts at: 0 116 2271
Size: 116 2155
DrdI GACnn_nn'nnGTC
Cuts at: 0 390 2271
Size: 390 1881
EaeI y'GGCC_r
Cuts at: 0 1472 1766 2167 2271
Size: 1472 294 401 104
Fragments arranged by size:
1472 401 294 104
EarI CTCTTCn'nnn_
Cuts at: 0 1700 2271
Size: 1700 571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 382 439 586 865 973 1942 2271
Size: 346 36 57 147 279 108 969 329
Fragments arranged by size:
969 346 329 279 147 108 57 36
EcoO109I rG'GnC_Cy
Cuts at: 0 948 2271
Size: 948 1323
FspI TGC'GCA
Cuts at: 0 1427 2271
Size: 1427 844
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 56 88 909 941 1217 1249 1280 1312
Size: 56 32 821 32 276 32 31 32
Cuts at: 1312 2271
Size: 959
Fragments arranged by size:
959 821 276 56 32 32 32 32
31
HincII GTy'rAC
Cuts at: 0 730 1508 2271
Size: 730 778 763
Fragments arranged by size:
778 763 730
HindIII A'AGCT_T
Cuts at: 0 418 2271
Size: 418 1853
HpaI GTT'AAC
Cuts at: 0 1508 2271
Size: 1508 763
KpnI G_GTAC'C
Cuts at: 0 1979 2271
Size: 1979 292
MfeI C'AATT_G
Cuts at: 0 967 2271
Size: 967 1304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1098 2271
Size: 1098 1173
MscI TGG'CCA
Cuts at: 0 1474 2169 2271
Size: 1474 695 102
Fragments arranged by size:
1474 695 102
MslI CAynn'nnrTG
Cuts at: 0 59 279 399 857 1282 1823 2155 2271
Size: 59 220 120 458 425 541 332 116
Fragments arranged by size:
541 458 425 332 220 120 116 59
MspA1I CmG'CkG
Cuts at: 0 1163 1431 2271
Size: 1163 268 840
Fragments arranged by size:
1163 840 268
NdeI CA'TA_TG
Cuts at: 0 2136 2271
Size: 2136 135
NsiI A_TGCA'T
Cuts at: 0 1914 2271
Size: 1914 357
NspI r_CATG'y
Cuts at: 0 738 1156 1287 1426 1806 2271
Size: 738 418 131 139 380 465
Fragments arranged by size:
738 465 418 380 139 131
PciI A'CATG_T
Cuts at: 0 1152 2271
Size: 1152 1119
PflMI CCAn_nnn'nTGG
Cuts at: 0 324 888 2271
Size: 324 564 1383
Fragments arranged by size:
1383 564 324
PpuMI rG'GwC_Cy
Cuts at: 0 948 2271
Size: 948 1323
PstI C_TGCA'G
Cuts at: 0 811 1251 2177 2271
Size: 811 440 926 94
Fragments arranged by size:
926 811 440 94
PvuII CAG'CTG
Cuts at: 0 1163 1431 2271
Size: 1163 268 840
Fragments arranged by size:
1163 840 268
ScaI AGT'ACT
Cuts at: 0 1135 1446 2271
Size: 1135 311 825
Fragments arranged by size:
1135 825 311
SexAI A'CCwGG_T
Cuts at: 0 1971 2271
Size: 1971 300
SfcI C'TryA_G
Cuts at: 0 807 1247 2094 2173 2271
Size: 807 440 847 79 98
Fragments arranged by size:
847 807 440 98 79
SmlI C'TyrA_G
Cuts at: 0 1033 2271
Size: 1033 1238
SphI G_CATG'C
Cuts at: 0 1287 1426 2271
Size: 1287 139 845
Fragments arranged by size:
1287 845 139
SspI AAT'ATT
Cuts at: 0 46 223 2271
Size: 46 177 2048
Fragments arranged by size:
2048 177 46
StyI C'CwwG_G
Cuts at: 0 84 318 2271
Size: 84 234 1953
Fragments arranged by size:
1953 234 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2145 2271
Size: 2145 126
TatI w'GTAC_w
Cuts at: 0 1133 1444 2271
Size: 1133 311 827
Fragments arranged by size:
1133 827 311
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 61 93 2271
Size: 61 32 2178
Fragments arranged by size:
2178 61 32
XbaI T'CTAG_A
Cuts at: 0 1194 2271
Size: 1194 1077
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 746 2150 2271
Size: 746 1404 121
Fragments arranged by size:
1404 746 121
XmnI GAAnn'nnTTC
Cuts at: 0 587 926 2072 2271
Size: 587 339 1146 199
Fragments arranged by size:
1146 587 339 199
Enzymes that do cut:
Acc65I AclI AcuI AflIII AhdI AleI AlfI AlwNI
ApoI AseI BaeI BanI BanII BbsI BbvCI BciVI
BfrBI BglII BpmI Bpu10I BpuEI BsaI BsaXI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrDI BstAPI BstXI BstYI
BtsI DraI DraIII DrdI EaeI EarI Eco57MI EcoO109I
FspI Hin4I HincII HindIII HpaI KpnI MfeI MmeI
MscI MslI MspA1I NdeI NsiI NspI PciI PflMI
PpuMI PstI PvuII ScaI SexAI SfcI SmlI SphI
SspI StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AgeI AloI ApaI
ApaLI AscI AsiSI AvaI AvrII BamHI BbeI BcgI
BclI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspAI HaeII KasI MluI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PasI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI
Tth111I XhoI XmaI ZraI