Restriction map for
pFN21AE9541
(Linear) (Six-base) MAPSORT of: KIEE9541.seq Check: 3163 from: 1 to: 1344
KIEE9541.orf
With 182 enzymes: SgfI *
March 22, 2012 18:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 47 422 1344
Size: 47 375 922
Fragments arranged by size:
922 375 47
AccI GT'mk_AC
Cuts at: 0 1328 1344
Size: 1328 16
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 1344
Size: 602 742
AflIII A'CryG_T
Cuts at: 0 1301 1344
Size: 1301 43
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 1344
Size: 295 1049
AlwNI CAG_nnn'CTG
Cuts at: 0 258 691 805 1344
Size: 258 433 114 539
Fragments arranged by size:
539 433 258 114
AvaI C'yCGr_G
Cuts at: 0 216 508 1344
Size: 216 292 836
Fragments arranged by size:
836 292 216
BamHI G'GATC_C
Cuts at: 0 706 1344
Size: 706 638
BanI G'GyrC_C
Cuts at: 0 296 535 784 835 926 1108 1264 1270
Size: 296 239 249 51 91 182 156 6
Cuts at: 1270 1344
Size: 74
Fragments arranged by size:
296 249 239 182 156 91 74 51
6
BanII G_rGCy'C
Cuts at: 0 67 685 1090 1255 1344
Size: 67 618 405 165 89
Fragments arranged by size:
618 405 165 89 67
BbeI G_GCGC'C
Cuts at: 0 930 1344
Size: 930 414
BglI GCCn_nnn'nGGC
Cuts at: 0 283 313 924 1344
Size: 283 30 611 420
Fragments arranged by size:
611 420 283 30
Bme1580I G_kGCm'C
Cuts at: 0 274 465 517 597 1111 1344
Size: 274 191 52 80 514 233
Fragments arranged by size:
514 274 233 191 80 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 304 712 1344
Size: 304 408 632
Fragments arranged by size:
632 408 304
Bpu10I CC'TnA_GC
Cuts at: 0 406 563 684 1084 1344
Size: 406 157 121 400 260
Fragments arranged by size:
406 400 260 157 121
BsaI GGTCTCn'nnnn_
Cuts at: 0 460 643 1344
Size: 460 183 701
Fragments arranged by size:
701 460 183
BsaAI yAC'GTr
Cuts at: 0 303 1302 1344
Size: 303 999 42
Fragments arranged by size:
999 303 42
BsaHI Gr'CG_yC
Cuts at: 0 927 1344
Size: 927 417
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 736 1344
Size: 736 608
BseYI C'CCAG_C
Cuts at: 0 15 168 846 954 1041 1344
Size: 15 153 678 108 87 303
Fragments arranged by size:
678 303 153 108 87 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1026 1344
Size: 919 107 318
Fragments arranged by size:
919 318 107
BsiEI CG_ry'CG
Cuts at: 0 1216 1344
Size: 1216 128
Bsp1286I G_dGCh'C
Cuts at: 0 67 274 465 517 597 685 1090 1111
Size: 67 207 191 52 80 88 405 21
Cuts at: 1111 1255 1344
Size: 144 89
Fragments arranged by size:
405 207 191 144 89 88 80 67
52 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 47 222 422 998 1344
Size: 47 175 200 576 346
Fragments arranged by size:
576 346 200 175 47
BsrDI GCAATG_nn'
Cuts at: 0 371 754 1219 1344
Size: 371 383 465 125
Fragments arranged by size:
465 383 371 125
BsrFI r'CCGG_y
Cuts at: 0 1215 1267 1344
Size: 1215 52 77
Fragments arranged by size:
1215 77 52
BstAPI GCAn_nnn'nTGC
Cuts at: 0 601 1344
Size: 601 743
BstEII G'GTnAC_C
Cuts at: 0 192 1344
Size: 192 1152
BstXI CCAn_nnnn'nTGG
Cuts at: 0 131 246 1248 1344
Size: 131 115 1002 96
Fragments arranged by size:
1002 131 115 96
BstYI r'GATC_y
Cuts at: 0 86 706 1344
Size: 86 620 638
Fragments arranged by size:
638 620 86
Bsu36I CC'TnA_GG
Cuts at: 0 806 1344
Size: 806 538
BtgI C'CryG_G
Cuts at: 0 831 992 1344
Size: 831 161 352
Fragments arranged by size:
831 352 161
BtsI GCAGTG_nn'
Cuts at: 0 966 1112 1344
Size: 966 146 232
Fragments arranged by size:
966 232 146
DraIII CAC_nnn'GTG
Cuts at: 0 303 333 1344
Size: 303 30 1011
Fragments arranged by size:
1011 303 30
DrdI GACnn_nn'nnGTC
Cuts at: 0 808 1344
Size: 808 536
EaeI y'GGCC_r
Cuts at: 0 989 1213 1238 1344
Size: 989 224 25 106
Fragments arranged by size:
989 224 106 25
EagI C'GGCC_G
Cuts at: 0 1213 1344
Size: 1213 131
EarI CTCTTCn'nnn_
Cuts at: 0 722 737 1344
Size: 722 15 607
Fragments arranged by size:
722 607 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 1344
Size: 602 742
EcoNI CCTnn'n_nnAGG
Cuts at: 0 124 448 1344
Size: 124 324 896
Fragments arranged by size:
896 324 124
EcoO109I rG'GnC_Cy
Cuts at: 0 234 688 1010 1058 1344
Size: 234 454 322 48 286
Fragments arranged by size:
454 322 286 234 48
HaeII r_GCGC'y
Cuts at: 0 169 526 930 1344
Size: 169 357 404 414
Fragments arranged by size:
414 404 357 169
KasI G'GCGC_C
Cuts at: 0 926 1344
Size: 926 418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 1344
Size: 112 1232
MscI TGG'CCA
Cuts at: 0 991 1240 1344
Size: 991 249 104
Fragments arranged by size:
991 249 104
MslI CAynn'nnrTG
Cuts at: 0 244 997 1246 1344
Size: 244 753 249 98
Fragments arranged by size:
753 249 244 98
MspA1I CmG'CkG
Cuts at: 0 622 819 1231 1344
Size: 622 197 412 113
Fragments arranged by size:
622 412 197 113
NaeI GCC'GGC
Cuts at: 0 1217 1344
Size: 1217 127
NarI GG'CG_CC
Cuts at: 0 927 1344
Size: 927 417
NcoI C'CATG_G
Cuts at: 0 831 992 1344
Size: 831 161 352
Fragments arranged by size:
831 352 161
NdeI CA'TA_TG
Cuts at: 0 1170 1344
Size: 1170 174
NgoMIV G'CCGG_C
Cuts at: 0 1215 1344
Size: 1215 129
NspI r_CATG'y
Cuts at: 0 1050 1344
Size: 1050 294
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 333 1344
Size: 42 291 1011
Fragments arranged by size:
1011 291 42
PmlI CAC'GTG
Cuts at: 0 303 1302 1344
Size: 303 999 42
Fragments arranged by size:
999 303 42
PpuMI rG'GwC_Cy
Cuts at: 0 234 1010 1344
Size: 234 776 334
Fragments arranged by size:
776 334 234
PspXI vC'TCGA_Gb
Cuts at: 0 508 1344
Size: 508 836
PstI C_TGCA'G
Cuts at: 0 1119 1344
Size: 1119 225
PvuII CAG'CTG
Cuts at: 0 622 819 1231 1344
Size: 622 197 412 113
Fragments arranged by size:
622 412 197 113
SfcI C'TryA_G
Cuts at: 0 1115 1344
Size: 1115 229
SfoI GGC'GCC
Cuts at: 0 928 1344
Size: 928 416
SmaI CCC'GGG
Cuts at: 0 218 1344
Size: 218 1126
SmlI C'TyrA_G
Cuts at: 0 508 1344
Size: 508 836
SphI G_CATG'C
Cuts at: 0 1050 1344
Size: 1050 294
StyI C'CwwG_G
Cuts at: 0 353 831 992 1344
Size: 353 478 161 352
Fragments arranged by size:
478 353 352 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 24 81 1134 1344
Size: 24 57 1053 210
Fragments arranged by size:
1053 210 57 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1064 1096 1344
Size: 1064 32 248
Fragments arranged by size:
1064 248 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1119 1344
Size: 1119 225
XhoI C'TCGA_G
Cuts at: 0 508 1344
Size: 508 836
XmaI C'CCGG_G
Cuts at: 0 216 1344
Size: 216 1128
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI AvaI BamHI
BanI BanII BbeI BglI Bme1580I BmrI Bpu10I BsaI
BsaAI BsaHI BseRI BseYI BsgI BsiEI Bsp1286I BspMI
BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI DraIII DrdI EaeI EagI EarI Eco57MI EcoNI
EcoO109I HaeII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PflMI PmlI PpuMI
PspXI PstI PvuII SfcI SfoI SmaI SmlI SphI
StyI TaqII TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI BpmI BpuEI BsaBI BsaWI
BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI
DraI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI TatI Tth111I XbaI XmnI ZraI