Restriction map for
pFN21AE9540
(Linear) (Six-base) MAPSORT of: KIEE9540.seq Check: 6969 from: 1 to: 1290
KIEE9540.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1268 1290
Size: 1268 22
AccI GT'mk_AC
Cuts at: 0 644 923 1290
Size: 644 279 367
Fragments arranged by size:
644 367 279
AflIII A'CryG_T
Cuts at: 0 236 1290
Size: 236 1054
AleI CACnn'nnGTG
Cuts at: 0 301 400 652 1290
Size: 301 99 252 638
Fragments arranged by size:
638 301 252 99
ApaI G_GGCC'C
Cuts at: 0 501 745 1165 1179 1290
Size: 501 244 420 14 111
Fragments arranged by size:
501 420 244 111 14
ApaLI G'TGCA_C
Cuts at: 0 436 805 1290
Size: 436 369 485
Fragments arranged by size:
485 436 369
AvaI C'yCGr_G
Cuts at: 0 622 738 751 1290
Size: 622 116 13 539
Fragments arranged by size:
622 539 116 13
BanI G'GyrC_C
Cuts at: 0 678 1290
Size: 678 612
BanII G_rGCy'C
Cuts at: 0 14 501 745 1165 1179 1290
Size: 14 487 244 420 14 111
Fragments arranged by size:
487 420 244 111 14 14
BbeI G_GCGC'C
Cuts at: 0 682 1290
Size: 682 608
BbsI GAAGACnn'nnnn_
Cuts at: 0 1107 1290
Size: 1107 183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 748 782 1290
Size: 748 34 508
Fragments arranged by size:
748 508 34
BfrBI ATG'CAT
Cuts at: 0 222 1290
Size: 222 1068
BglI GCCn_nnn'nGGC
Cuts at: 0 76 1095 1290
Size: 76 1019 195
Fragments arranged by size:
1019 195 76
BlpI GC'TnA_GC
Cuts at: 0 892 1290
Size: 892 398
Bme1580I G_kGCm'C
Cuts at: 0 440 501 745 809 1165 1179 1290
Size: 440 61 244 64 356 14 111
Fragments arranged by size:
440 356 244 111 64 61 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 329 1290
Size: 329 961
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1095 1290
Size: 1095 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1290
Size: 190 1100
BsaHI Gr'CG_yC
Cuts at: 0 575 679 1290
Size: 575 104 611
Fragments arranged by size:
611 575 104
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 87 568 775 1290
Size: 87 481 207 515
Fragments arranged by size:
515 481 207 87
BseYI C'CCAG_C
Cuts at: 0 17 608 1132 1290
Size: 17 591 524 158
Fragments arranged by size:
591 524 158 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 892 1087 1290
Size: 385 507 195 203
Fragments arranged by size:
507 385 203 195
BsiEI CG_ry'CG
Cuts at: 0 530 1191 1290
Size: 530 661 99
Fragments arranged by size:
661 530 99
BsiHKAI G_wGCw'C
Cuts at: 0 206 440 560 659 809 1290
Size: 206 234 120 99 150 481
Fragments arranged by size:
481 234 206 150 120 99
Bsp1286I G_dGCh'C
Cuts at: 0 14 206 440 501 560 659 745 809
Size: 14 192 234 61 59 99 86 64
Cuts at: 809 1165 1179 1290
Size: 356 14 111
Fragments arranged by size:
356 234 192 111 99 86 64 61
59 14 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 275 1268 1290
Size: 275 993 22
Fragments arranged by size:
993 275 22
BsrBI CCG'CTC
Cuts at: 0 356 587 597 855 1086 1290
Size: 356 231 10 258 231 204
Fragments arranged by size:
356 258 231 231 204 10
BsrDI GCAATG_nn'
Cuts at: 0 407 1290
Size: 407 883
BsrFI r'CCGG_y
Cuts at: 0 212 1290
Size: 212 1078
BssHII G'CGCG_C
Cuts at: 0 718 796 896 1290
Size: 718 78 100 394
Fragments arranged by size:
718 394 100 78
BstAPI GCAn_nnn'nTGC
Cuts at: 0 284 728 1290
Size: 284 444 562
Fragments arranged by size:
562 444 284
BstEII G'GTnAC_C
Cuts at: 0 915 1290
Size: 915 375
BstXI CCAn_nnnn'nTGG
Cuts at: 0 303 414 1290
Size: 303 111 876
Fragments arranged by size:
876 303 111
BtgI C'CryG_G
Cuts at: 0 1011 1224 1290
Size: 1011 213 66
Fragments arranged by size:
1011 213 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 468 699 767 1290
Size: 468 231 68 523
Fragments arranged by size:
523 468 231 68
BtsI GCAGTG_nn'
Cuts at: 0 1176 1290
Size: 1176 114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 282 317 1290
Size: 282 35 973
Fragments arranged by size:
973 282 35
DraIII CAC_nnn'GTG
Cuts at: 0 323 1290
Size: 323 967
EaeI y'GGCC_r
Cuts at: 0 128 404 527 1188 1290
Size: 128 276 123 661 102
Fragments arranged by size:
661 276 128 123 102
EagI C'GGCC_G
Cuts at: 0 527 1188 1290
Size: 527 661 102
Fragments arranged by size:
661 527 102
EarI CTCTTCn'nnn_
Cuts at: 0 664 1290
Size: 664 626
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 997 1290
Size: 997 293
EcoNI CCTnn'n_nnAGG
Cuts at: 0 466 1290
Size: 466 824
EcoO109I rG'GnC_Cy
Cuts at: 0 498 735 1162 1175 1176 1243 1290
Size: 498 237 427 13 1 67 47
Fragments arranged by size:
498 427 237 67 47 13 1
EcoRV GAT'ATC
Cuts at: 0 1287 1290
Size: 1287 3
FspI TGC'GCA
Cuts at: 0 637 1290
Size: 637 653
HaeII r_GCGC'y
Cuts at: 0 475 508 682 691 829 865 971 1290
Size: 475 33 174 9 138 36 106 319
Fragments arranged by size:
475 319 174 138 106 36 33 9
KasI G'GCGC_C
Cuts at: 0 678 1290
Size: 678 612
MscI TGG'CCA
Cuts at: 0 406 1290
Size: 406 884
MslI CAynn'nnrTG
Cuts at: 0 235 301 376 400 412 652 1290
Size: 235 66 75 24 12 240 638
Fragments arranged by size:
638 240 235 75 66 24 12
MspA1I CmG'CkG
Cuts at: 0 428 684 906 1013 1200 1290
Size: 428 256 222 107 187 90
Fragments arranged by size:
428 256 222 187 107 90
NarI GG'CG_CC
Cuts at: 0 679 1290
Size: 679 611
NsiI A_TGCA'T
Cuts at: 0 224 1290
Size: 224 1066
NspI r_CATG'y
Cuts at: 0 222 240 291 347 375 636 813 1290
Size: 222 18 51 56 28 261 177 477
Fragments arranged by size:
477 261 222 177 56 51 28 18
PasI CC'CwG_GG
Cuts at: 0 1235 1290
Size: 1235 55
PciI A'CATG_T
Cuts at: 0 236 1290
Size: 236 1054
PflMI CCAn_nnn'nTGG
Cuts at: 0 567 1290
Size: 567 723
PspOMI G'GGCC_C
Cuts at: 0 497 741 1161 1175 1290
Size: 497 244 420 14 115
Fragments arranged by size:
497 420 244 115 14
PvuII CAG'CTG
Cuts at: 0 906 1290
Size: 906 384
SacII CC_GC'GG
Cuts at: 0 1014 1290
Size: 1014 276
SapI GCTCTTCn'nnn_
Cuts at: 0 664 1290
Size: 664 626
SexAI A'CCwGG_T
Cuts at: 0 911 1290
Size: 911 379
SfoI GGC'GCC
Cuts at: 0 680 1290
Size: 680 610
SmaI CCC'GGG
Cuts at: 0 740 753 1290
Size: 740 13 537
Fragments arranged by size:
740 537 13
SmlI C'TyrA_G
Cuts at: 0 205 1290
Size: 205 1085
SphI G_CATG'C
Cuts at: 0 291 636 1290
Size: 291 345 654
Fragments arranged by size:
654 345 291
StuI AGG'CCT
Cuts at: 0 616 1075 1290
Size: 616 459 215
Fragments arranged by size:
616 459 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 311 1290
Size: 311 979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 138 300 1290
Size: 138 162 990
Fragments arranged by size:
990 162 138
XmaI C'CCGG_G
Cuts at: 0 738 751 1290
Size: 738 13 539
Fragments arranged by size:
738 539 13
Enzymes that do cut:
AarI AccI AflIII AleI ApaI ApaLI AvaI BanI
BanII BbeI BbsI BcgI BfrBI BglI BlpI Bme1580I
BmrI BplI BpuEI BsaHI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI
BstEII BstXI BtgI BtgZI BtsI CspCI DraIII EaeI
EagI EarI EciI EcoNI EcoO109I EcoRV FspI HaeII
KasI MscI MslI MspA1I NarI NsiI NspI PasI
PciI PflMI PspOMI PvuII SacII SapI SexAI SfoI
SmaI SmlI SphI StuI TaqII XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BclI BglII BmgBI BmtI
BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BsiWI
BsmI BsmBI BspEI BspHI BsrGI BssSI BstBI BstYI
BstZ17I Bsu36I ClaI DraI DrdI EcoICRI Eco57MI EcoRI
FalI FseI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI NaeI NcoI NdeI NgoMIV NheI
NotI NruI PacI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI StyI SwaI TatI TstI
Tth111I XbaI XhoI XmnI ZraI