Restriction map for
pFN21AE9538
(Linear) (Six-base) MAPSORT of: KIEE9538.seq Check: 4990 from: 1 to: 984
KIEE9538.orf
With 182 enzymes: SgfI *
March 22, 2012 18:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 516 630 808 984
Size: 163 353 114 178 176
Fragments arranged by size:
353 178 176 163 114
AleI CACnn'nnGTG
Cuts at: 0 612 984
Size: 612 372
ApoI r'AATT_y
Cuts at: 0 860 984
Size: 860 124
AvrII C'CTAG_G
Cuts at: 0 855 984
Size: 855 129
BamHI G'GATC_C
Cuts at: 0 491 984
Size: 491 493
BbsI GAAGACnn'nnnn_
Cuts at: 0 796 984
Size: 796 188
BclI T'GATC_A
Cuts at: 0 401 984
Size: 401 583
BmrI ACTGGGnnnn_n'
Cuts at: 0 901 984
Size: 901 83
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 352 984
Size: 352 632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 984
Size: 347 637
Bpu10I CC'TnA_GC
Cuts at: 0 252 984
Size: 252 732
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 984
Size: 204 780
BseYI C'CCAG_C
Cuts at: 0 381 984
Size: 381 603
BsiHKAI G_wGCw'C
Cuts at: 0 783 984
Size: 783 201
Bsp1286I G_dGCh'C
Cuts at: 0 783 984
Size: 783 201
BspHI T'CATG_A
Cuts at: 0 389 984
Size: 389 595
BsrBI CCG'CTC
Cuts at: 0 416 984
Size: 416 568
BsrDI GCAATG_nn'
Cuts at: 0 696 984
Size: 696 288
BssSI C'ACGA_G
Cuts at: 0 429 984
Size: 429 555
BstAPI GCAn_nnn'nTGC
Cuts at: 0 10 984
Size: 10 974
BstXI CCAn_nnnn'nTGG
Cuts at: 0 264 984
Size: 264 720
BstYI r'GATC_y
Cuts at: 0 107 491 984
Size: 107 384 493
Fragments arranged by size:
493 384 107
BtgI C'CryG_G
Cuts at: 0 473 984
Size: 473 511
BtsI GCAGTG_nn'
Cuts at: 0 594 984
Size: 594 390
DraI TTT'AAA
Cuts at: 0 941 984
Size: 941 43
EarI CTCTTCn'nnn_
Cuts at: 0 661 984
Size: 661 323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 347 516 630 808 984
Size: 163 184 169 114 178 176
Fragments arranged by size:
184 178 176 169 163 114
EcoNI CCTnn'n_nnAGG
Cuts at: 0 727 984
Size: 727 257
EcoO109I rG'GnC_Cy
Cuts at: 0 434 720 732 984
Size: 434 286 12 252
Fragments arranged by size:
434 286 252 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 252 984
Size: 252 732
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 528 560 984
Size: 528 32 424
Fragments arranged by size:
528 424 32
HindIII A'AGCT_T
Cuts at: 0 361 984
Size: 361 623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 444 984
Size: 245 199 540
Fragments arranged by size:
540 245 199
MslI CAynn'nnrTG
Cuts at: 0 394 612 984
Size: 394 218 372
Fragments arranged by size:
394 372 218
MspA1I CmG'CkG
Cuts at: 0 650 984
Size: 650 334
NcoI C'CATG_G
Cuts at: 0 473 984
Size: 473 511
PflMI CCAn_nnn'nTGG
Cuts at: 0 292 984
Size: 292 692
PpuMI rG'GwC_Cy
Cuts at: 0 720 732 984
Size: 720 12 252
Fragments arranged by size:
720 252 12
PshAI GACnn'nnGTC
Cuts at: 0 718 984
Size: 718 266
PsiI TTA'TAA
Cuts at: 0 632 984
Size: 632 352
PstI C_TGCA'G
Cuts at: 0 601 984
Size: 601 383
PvuII CAG'CTG
Cuts at: 0 650 984
Size: 650 334
SfcI C'TryA_G
Cuts at: 0 597 984
Size: 597 387
SmlI C'TyrA_G
Cuts at: 0 219 984
Size: 219 765
StyI C'CwwG_G
Cuts at: 0 473 855 984
Size: 473 382 129
Fragments arranged by size:
473 382 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 283 315 984
Size: 283 32 669
Fragments arranged by size:
669 283 32
Tth111I GACn'n_nGTC
Cuts at: 0 96 984
Size: 96 888
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 322 480 984
Size: 322 158 504
Fragments arranged by size:
504 322 158
Enzymes that do cut:
AcuI AleI ApoI AvrII BamHI BbsI BclI BmrI
BplI BpmI Bpu10I BpuEI BseYI BsiHKAI Bsp1286I BspHI
BsrBI BsrDI BssSI BstAPI BstXI BstYI BtgI BtsI
DraI EarI Eco57MI EcoNI EcoO109I FalI Hin4I HindIII
MmeI MslI MspA1I NcoI PflMI PpuMI PshAI PsiI
PstI PvuII SfcI SmlI StyI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI BaeI BanI BanII BbeI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrFI
BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI XbaI
XhoI XmaI XmnI ZraI