Restriction map for
pFN21AE9537
(Linear) (Six-base) MAPSORT of: KIEE9537.seq Check: 2690 from: 1 to: 972
KIEE9537.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 677 779 972
Size: 232 445 102 193
Fragments arranged by size:
445 232 193 102
AflIII A'CryG_T
Cuts at: 0 48 972
Size: 48 924
AlwNI CAG_nnn'CTG
Cuts at: 0 16 448 700 811 972
Size: 16 432 252 111 161
Fragments arranged by size:
432 252 161 111 16
ApaI G_GGCC'C
Cuts at: 0 503 972
Size: 503 469
ApoI r'AATT_y
Cuts at: 0 852 972
Size: 852 120
AvaI C'yCGr_G
Cuts at: 0 236 972
Size: 236 736
BanI G'GyrC_C
Cuts at: 0 85 972
Size: 85 887
BanII G_rGCy'C
Cuts at: 0 464 503 972
Size: 464 39 469
Fragments arranged by size:
469 464 39
BbeI G_GCGC'C
Cuts at: 0 89 972
Size: 89 883
BbsI GAAGACnn'nnnn_
Cuts at: 0 72 972
Size: 72 900
BlpI GC'TnA_GC
Cuts at: 0 20 972
Size: 20 952
Bme1580I G_kGCm'C
Cuts at: 0 503 972
Size: 503 469
BmgBI CAC'GTC
Cuts at: 0 51 972
Size: 51 921
BmrI ACTGGGnnnn_n'
Cuts at: 0 324 426 972
Size: 324 102 546
Fragments arranged by size:
546 324 102
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 130 972
Size: 130 842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 972
Size: 160 812
BsaHI Gr'CG_yC
Cuts at: 0 86 972
Size: 86 886
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 236 266 972
Size: 236 30 706
Fragments arranged by size:
706 236 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 824 972
Size: 278 546 148
Fragments arranged by size:
546 278 148
BsiEI CG_ry'CG
Cuts at: 0 294 707 972
Size: 294 413 265
Fragments arranged by size:
413 294 265
BsiHKAI G_wGCw'C
Cuts at: 0 176 972
Size: 176 796
BsmI GAATG_Cn'
Cuts at: 0 573 972
Size: 573 399
Bsp1286I G_dGCh'C
Cuts at: 0 176 464 503 972
Size: 176 288 39 469
Fragments arranged by size:
469 288 176 39
BsrBI CCG'CTC
Cuts at: 0 233 972
Size: 233 739
BstAPI GCAn_nnn'nTGC
Cuts at: 0 16 972
Size: 16 956
BstYI r'GATC_y
Cuts at: 0 605 972
Size: 605 367
BtgI C'CryG_G
Cuts at: 0 167 200 972
Size: 167 33 772
Fragments arranged by size:
772 167 33
DrdI GACnn_nn'nnGTC
Cuts at: 0 49 972
Size: 49 923
EaeI y'GGCC_r
Cuts at: 0 573 972
Size: 573 399
EarI CTCTTCn'nnn_
Cuts at: 0 547 590 972
Size: 547 43 382
Fragments arranged by size:
547 382 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 677 779 972
Size: 232 445 102 193
Fragments arranged by size:
445 232 193 102
EcoO109I rG'GnC_Cy
Cuts at: 0 187 396 499 500 785 972
Size: 187 209 103 1 285 187
Fragments arranged by size:
285 209 187 187 103 1
EcoRI G'AATT_C
Cuts at: 0 852 972
Size: 852 120
HaeII r_GCGC'y
Cuts at: 0 89 972
Size: 89 883
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 287 319 811 843 972
Size: 287 32 492 32 129
Fragments arranged by size:
492 287 129 32 32
KasI G'GCGC_C
Cuts at: 0 85 972
Size: 85 887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 972
Size: 437 535
MscI TGG'CCA
Cuts at: 0 575 972
Size: 575 397
MspA1I CmG'CkG
Cuts at: 0 13 972
Size: 13 959
NarI GG'CG_CC
Cuts at: 0 86 972
Size: 86 886
NcoI C'CATG_G
Cuts at: 0 167 972
Size: 167 805
NspI r_CATG'y
Cuts at: 0 140 197 372 972
Size: 140 57 175 600
Fragments arranged by size:
600 175 140 57
PflMI CCAn_nnn'nTGG
Cuts at: 0 615 972
Size: 615 357
PfoI T'CCnGG_A
Cuts at: 0 717 972
Size: 717 255
PpuMI rG'GwC_Cy
Cuts at: 0 396 785 972
Size: 396 389 187
Fragments arranged by size:
396 389 187
PspOMI G'GGCC_C
Cuts at: 0 499 972
Size: 499 473
PstI C_TGCA'G
Cuts at: 0 12 269 491 933 972
Size: 12 257 222 442 39
Fragments arranged by size:
442 257 222 39 12
PvuII CAG'CTG
Cuts at: 0 13 972
Size: 13 959
SanDI GG'GwC_CC
Cuts at: 0 396 972
Size: 396 576
SfcI C'TryA_G
Cuts at: 0 8 265 487 635 929 972
Size: 8 257 222 148 294 43
Fragments arranged by size:
294 257 222 148 43 8
SfoI GGC'GCC
Cuts at: 0 87 972
Size: 87 885
SmaI CCC'GGG
Cuts at: 0 238 972
Size: 238 734
SmlI C'TyrA_G
Cuts at: 0 175 972
Size: 175 797
SphI G_CATG'C
Cuts at: 0 140 372 972
Size: 140 232 600
Fragments arranged by size:
600 232 140
SspI AAT'ATT
Cuts at: 0 656 972
Size: 656 316
StuI AGG'CCT
Cuts at: 0 868 972
Size: 868 104
StyI C'CwwG_G
Cuts at: 0 167 972
Size: 167 805
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 308 972
Size: 308 664
XmaI C'CCGG_G
Cuts at: 0 236 972
Size: 236 736
Enzymes that do cut:
AcuI AflIII AlwNI ApaI ApoI AvaI BanI BanII
BbeI BbsI BlpI Bme1580I BmgBI BmrI BplI BpuEI
BsaHI BsaXI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI
BstAPI BstYI BtgI DrdI EaeI EarI Eco57MI EcoO109I
EcoRI HaeII Hin4I KasI MmeI MscI MspA1I NarI
NcoI NspI PflMI PfoI PpuMI PspOMI PstI PvuII
SanDI SfcI SfoI SmaI SmlI SphI SspI StuI
StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmtI BpmI Bpu10I BsaI BsaAI BsaBI
BsaWI BseRI BseYI BsiWI BsmBI BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MslI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI