Restriction map for
pFN21AE9536
(Linear) (Six-base) MAPSORT of: KIEE9536.seq Check: 8755 from: 1 to: 3645
KIEE9536.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2758 3645
Size: 2758 887
AccI GT'mk_AC
Cuts at: 0 1313 3645
Size: 1313 2332
Acc65I G'GTAC_C
Cuts at: 0 467 3645
Size: 467 3178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 516 913 1897 2369 3044 3227 3645
Size: 301 215 397 984 472 675 183 418
Fragments arranged by size:
984 675 472 418 397 301 215 183
AfeI AGC'GCT
Cuts at: 0 2643 3645
Size: 2643 1002
AhdI GACnn_n'nnGTC
Cuts at: 0 3047 3645
Size: 3047 598
AlwNI CAG_nnn'CTG
Cuts at: 0 477 1753 3645
Size: 477 1276 1892
Fragments arranged by size:
1892 1276 477
ApaI G_GGCC'C
Cuts at: 0 728 3645
Size: 728 2917
ApoI r'AATT_y
Cuts at: 0 895 1550 1650 1756 2535 2838 3645
Size: 895 655 100 106 779 303 807
Fragments arranged by size:
895 807 779 655 303 106 100
AvaI C'yCGr_G
Cuts at: 0 104 515 728 835 3645
Size: 104 411 213 107 2810
Fragments arranged by size:
2810 411 213 107 104
AvrII C'CTAG_G
Cuts at: 0 1026 2247 3610 3645
Size: 1026 1221 1363 35
Fragments arranged by size:
1363 1221 1026 35
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2079 2112 3645
Size: 2079 33 1533
Fragments arranged by size:
2079 1533 33
BamHI G'GATC_C
Cuts at: 0 2077 2127 3645
Size: 2077 50 1518
Fragments arranged by size:
2077 1518 50
BanI G'GyrC_C
Cuts at: 0 67 199 231 467 2326 2616 2675 2977
Size: 67 132 32 236 1859 290 59 302
Cuts at: 2977 3645
Size: 668
Fragments arranged by size:
1859 668 302 290 236 132 67 59
32
BanII G_rGCy'C
Cuts at: 0 506 728 767 1461 1829 1862 3645
Size: 506 222 39 694 368 33 1783
Fragments arranged by size:
1783 694 506 368 222 39 33
BbeI G_GCGC'C
Cuts at: 0 71 2330 3645
Size: 71 2259 1315
Fragments arranged by size:
2259 1315 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 561 1456 2375 2424 2594 2740 3645
Size: 561 895 919 49 170 146 905
Fragments arranged by size:
919 905 895 561 170 146 49
BbvCI CC'TCA_GC
Cuts at: 0 2653 3164 3645
Size: 2653 511 481
Fragments arranged by size:
2653 511 481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 130 164 275 309 3645
Size: 130 34 111 34 3336
Fragments arranged by size:
3336 130 111 34 34
BclI T'GATC_A
Cuts at: 0 2198 3645
Size: 2198 1447
BglI GCCn_nnn'nGGC
Cuts at: 0 840 3645
Size: 840 2805
BglII A'GATC_T
Cuts at: 0 3623 3645
Size: 3623 22
BlpI GC'TnA_GC
Cuts at: 0 44 3645
Size: 44 3601
Bme1580I G_kGCm'C
Cuts at: 0 236 728 2678 3645
Size: 236 492 1950 967
Fragments arranged by size:
1950 967 492 236
BmgBI CAC'GTC
Cuts at: 0 243 3645
Size: 243 3402
BmrI ACTGGGnnnn_n'
Cuts at: 0 2681 3645
Size: 2681 964
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 514 559 673 829 1103 2130 2203
Size: 201 313 45 114 156 274 1027 73
Cuts at: 2203 3260 3645
Size: 1057 385
Fragments arranged by size:
1057 1027 385 313 274 201 156 114
73 45
Bpu10I CC'TnA_GC
Cuts at: 0 2653 3164 3645
Size: 2653 511 481
Fragments arranged by size:
2653 511 481
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 939 1890 2425 3453 3645
Size: 939 951 535 1028 192
Fragments arranged by size:
1028 951 939 535 192
BsaI GGTCTCn'nnnn_
Cuts at: 0 649 800 2407 2468 3523 3645
Size: 649 151 1607 61 1055 122
Fragments arranged by size:
1607 1055 649 151 122 61
BsaAI yAC'GTr
Cuts at: 0 2469 3084 3645
Size: 2469 615 561
Fragments arranged by size:
2469 615 561
BsaBI GATnn'nnATC
Cuts at: 0 2076 3645
Size: 2076 1569
BsaHI Gr'CG_yC
Cuts at: 0 68 2327 3645
Size: 68 2259 1318
Fragments arranged by size:
2259 1318 68
BsaWI w'CCGG_w
Cuts at: 0 1119 3645
Size: 1119 2526
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 604 634 646 676 1101 1131 3645
Size: 604 30 12 30 425 30 2514
Fragments arranged by size:
2514 604 425 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 16 159 241 604 612 2425 2533 2536
Size: 16 143 82 363 8 1813 108 3
Cuts at: 2536 2908 3316 3457 3645
Size: 372 408 141 188
Fragments arranged by size:
1813 408 372 363 188 143 141 108
82 16 8 3
BseYI C'CCAG_C
Cuts at: 0 48 137 505 1730 2749 3340 3534 3645
Size: 48 89 368 1225 1019 591 194 111
Fragments arranged by size:
1225 1019 591 368 194 111 89 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3344 3645
Size: 3344 301
BsiEI CG_ry'CG
Cuts at: 0 94 303 3645
Size: 94 209 3342
Fragments arranged by size:
3342 209 94
BsiHKAI G_wGCw'C
Cuts at: 0 1862 2120 2236 2308 3645
Size: 1862 258 116 72 1337
Fragments arranged by size:
1862 1337 258 116 72
BsmI GAATG_Cn'
Cuts at: 0 1816 3645
Size: 1816 1829
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2259 3645
Size: 2259 1386
Bsp1286I G_dGCh'C
Cuts at: 0 236 506 728 767 1461 1829 1862 2120
Size: 236 270 222 39 694 368 33 258
Cuts at: 2120 2236 2308 2678 3645
Size: 116 72 370 967
Fragments arranged by size:
967 694 370 368 270 258 236 222
116 72 39 33
BspEI T'CCGG_A
Cuts at: 0 1119 3645
Size: 1119 2526
BspHI T'CATG_A
Cuts at: 0 908 1577 2261 3645
Size: 908 669 684 1384
Fragments arranged by size:
1384 908 684 669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2758 3579 3645
Size: 2758 821 66
Fragments arranged by size:
2758 821 66
BsrBI CCG'CTC
Cuts at: 0 188 3645
Size: 188 3457
BsrFI r'CCGG_y
Cuts at: 0 555 2297 3645
Size: 555 1742 1348
Fragments arranged by size:
1742 1348 555
BssHII G'CGCG_C
Cuts at: 0 350 3645
Size: 350 3295
BssSI C'ACGA_G
Cuts at: 0 2021 3320 3500 3645
Size: 2021 1299 180 145
Fragments arranged by size:
2021 1299 180 145
BstAPI GCAn_nnn'nTGC
Cuts at: 0 447 3645
Size: 447 3198
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 3645
Size: 243 3402
BstYI r'GATC_y
Cuts at: 0 1381 2077 2127 2279 2862 3623 3645
Size: 1381 696 50 152 583 761 22
Fragments arranged by size:
1381 761 696 583 152 50 22
Bsu36I CC'TnA_GG
Cuts at: 0 3155 3645
Size: 3155 490
BtgI C'CryG_G
Cuts at: 0 8 236 669 708 2334 2606 3645
Size: 8 228 433 39 1626 272 1039
Fragments arranged by size:
1626 1039 433 272 228 39 8
BtsI GCAGTG_nn'
Cuts at: 0 1802 2308 2351 2951 3645
Size: 1802 506 43 600 694
Fragments arranged by size:
1802 694 600 506 43
ClaI AT'CG_AT
Cuts at: 0 2689 3645
Size: 2689 956
DraI TTT'AAA
Cuts at: 0 3639 3645
Size: 3639 6
EaeI y'GGCC_r
Cuts at: 0 91 300 334 344 521 638 1677 3645
Size: 91 209 34 10 177 117 1039 1968
Fragments arranged by size:
1968 1039 209 177 117 91 34 10
EagI C'GGCC_G
Cuts at: 0 91 300 3645
Size: 91 209 3345
Fragments arranged by size:
3345 209 91
EarI CTCTTCn'nnn_
Cuts at: 0 2480 3582 3614 3645
Size: 2480 1102 32 31
Fragments arranged by size:
2480 1102 32 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 788 1583 3645
Size: 788 795 2062
Fragments arranged by size:
2062 795 788
EcoICRI GAG'CTC
Cuts at: 0 1860 3645
Size: 1860 1785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 301 514 516 559 673 829 913
Size: 201 100 213 2 43 114 156 84
Cuts at: 913 1103 1897 2130 2203 2369 3044 3227 3260
Size: 190 794 233 73 166 675 183 33
Cuts at: 3260 3645
Size: 385
Fragments arranged by size:
794 675 385 233 213 201 190 183
166 156 114 100 84 73 43 33
2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 493 3645
Size: 493 3152
EcoO109I rG'GnC_Cy
Cuts at: 0 113 692 724 725 732 2244 3607 3645
Size: 113 579 32 1 7 1512 1363 38
Fragments arranged by size:
1512 1363 579 113 38 32 7 1
EcoRI G'AATT_C
Cuts at: 0 895 1550 3645
Size: 895 655 2095
Fragments arranged by size:
2095 895 655
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 801 3015 3232 3581 3645
Size: 801 2214 217 349 64
Fragments arranged by size:
2214 801 349 217 64
HaeII r_GCGC'y
Cuts at: 0 71 376 478 2330 2645 2726 3645
Size: 71 305 102 1852 315 81 919
Fragments arranged by size:
1852 919 315 305 102 81 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 232 264 532 564 604 636 937 969
Size: 232 32 268 32 40 32 301 32
Cuts at: 969 1101 1133 1241 1273 1373 1405 2587 2619
Size: 132 32 108 32 100 32 1182 32
Cuts at: 2619 2847 2879 3033 3065 3522 3554 3645
Size: 228 32 154 32 457 32 91
Fragments arranged by size:
1182 457 301 268 232 228 154 132
108 100 91 40 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 918 2943 3645
Size: 918 2025 702
Fragments arranged by size:
2025 918 702
HindIII A'AGCT_T
Cuts at: 0 3256 3645
Size: 3256 389
KasI G'GCGC_C
Cuts at: 0 67 2326 3645
Size: 67 2259 1319
Fragments arranged by size:
2259 1319 67
KpnI G_GTAC'C
Cuts at: 0 471 3645
Size: 471 3174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1343 3645
Size: 1343 2302
MscI TGG'CCA
Cuts at: 0 336 346 640 1679 3645
Size: 336 10 294 1039 1966
Fragments arranged by size:
1966 1039 336 294 10
MslI CAynn'nnrTG
Cuts at: 0 241 1306 1582 1641 2986 3645
Size: 241 1065 276 59 1345 659
Fragments arranged by size:
1345 1065 659 276 241 59
MspA1I CmG'CkG
Cuts at: 0 48 279 710 746 1730 3163 3645
Size: 48 231 431 36 984 1433 482
Fragments arranged by size:
1433 984 482 431 231 48 36
NaeI GCC'GGC
Cuts at: 0 2299 3645
Size: 2299 1346
NarI GG'CG_CC
Cuts at: 0 68 2327 3645
Size: 68 2259 1318
Fragments arranged by size:
2259 1318 68
NcoI C'CATG_G
Cuts at: 0 8 236 2334 3645
Size: 8 228 2098 1311
Fragments arranged by size:
2098 1311 228 8
NdeI CA'TA_TG
Cuts at: 0 2046 3645
Size: 2046 1599
NgoMIV G'CCGG_C
Cuts at: 0 2297 3645
Size: 2297 1348
NspI r_CATG'y
Cuts at: 0 1305 3645
Size: 1305 2340
PflMI CCAn_nnn'nTGG
Cuts at: 0 477 1653 3550 3645
Size: 477 1176 1897 95
Fragments arranged by size:
1897 1176 477 95
PfoI T'CCnGG_A
Cuts at: 0 890 1080 1229 3645
Size: 890 190 149 2416
Fragments arranged by size:
2416 890 190 149
PmlI CAC'GTG
Cuts at: 0 2469 3645
Size: 2469 1176
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1482 1514 3645
Size: 1482 32 2131
Fragments arranged by size:
2131 1482 32
PpuMI rG'GwC_Cy
Cuts at: 0 113 3645
Size: 113 3532
PshAI GACnn'nnGTC
Cuts at: 0 1914 3645
Size: 1914 1731
PspOMI G'GGCC_C
Cuts at: 0 724 3645
Size: 724 2921
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 811 843 3645
Size: 811 32 2802
Fragments arranged by size:
2802 811 32
PstI C_TGCA'G
Cuts at: 0 422 3645
Size: 422 3223
PvuII CAG'CTG
Cuts at: 0 48 279 746 3163 3645
Size: 48 231 467 2417 482
Fragments arranged by size:
2417 482 467 231 48
SacI G_AGCT'C
Cuts at: 0 1862 3645
Size: 1862 1783
SacII CC_GC'GG
Cuts at: 0 711 3645
Size: 711 2934
SexAI A'CCwGG_T
Cuts at: 0 884 3645
Size: 884 2761
SfcI C'TryA_G
Cuts at: 0 418 2036 3371 3645
Size: 418 1618 1335 274
Fragments arranged by size:
1618 1335 418 274
SfoI GGC'GCC
Cuts at: 0 69 2328 3645
Size: 69 2259 1317
Fragments arranged by size:
2259 1317 69
SmaI CCC'GGG
Cuts at: 0 517 730 837 3645
Size: 517 213 107 2808
Fragments arranged by size:
2808 517 213 107
SmlI C'TyrA_G
Cuts at: 0 104 954 1905 2440 3468 3645
Size: 104 850 951 535 1028 177
Fragments arranged by size:
1028 951 850 535 177 104
SphI G_CATG'C
Cuts at: 0 1305 3645
Size: 1305 2340
StuI AGG'CCT
Cuts at: 0 1025 1274 3645
Size: 1025 249 2371
Fragments arranged by size:
2371 1025 249
StyI C'CwwG_G
Cuts at: 0 8 84 236 1026 1269 2247 2334 2405
Size: 8 76 152 790 243 978 87 71
Cuts at: 2405 2726 3022 3228 3517 3610 3645
Size: 321 296 206 289 93 35
Fragments arranged by size:
978 790 321 296 289 243 206 152
93 87 76 71 35 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 673 2693 2998 3645
Size: 673 2020 305 647
Fragments arranged by size:
2020 673 647 305
TatI w'GTAC_w
Cuts at: 0 1141 1415 3645
Size: 1141 274 2230
Fragments arranged by size:
2230 1141 274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 554 586 646 678 3645
Size: 554 32 60 32 2967
Fragments arranged by size:
2967 554 60 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 648 3645
Size: 648 2997
XhoI C'TCGA_G
Cuts at: 0 104 3645
Size: 104 3541
XmaI C'CCGG_G
Cuts at: 0 515 728 835 3645
Size: 515 213 107 2810
Fragments arranged by size:
2810 515 213 107
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AhdI AlwNI ApaI
ApoI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI
ClaI DraI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PflMI PfoI PmlI PpiI
PpuMI PshAI PspOMI PsrI PstI PvuII SacI SacII
SexAI SfcI SfoI SmaI SmlI SphI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AleI AlfI AloI
ApaLI AscI AseI AsiSI BciVI BfrBI BmtI BplI
BsiWI BsrDI BsrGI BstBI BstEII BstZ17I BtgZI CspCI
DraIII DrdI EcoRV FseI FspI FspAI HpaI MfeI
MluI NheI NotI NruI NsiI PacI PasI PciI
PmeI PsiI PspXI PvuI RsrII SalI SanDI SapI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XmnI ZraI