Restriction map for
pFN21AE9531
(Linear) (Six-base) MAPSORT of: KIEE9531.seq Check: 1140 from: 1 to: 885
KIEE9531.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 251 320 618 885
Size: 251 69 298 267
Fragments arranged by size:
298 267 251 69
ApaI G_GGCC'C
Cuts at: 0 688 885
Size: 688 197
AvaI C'yCGr_G
Cuts at: 0 40 885
Size: 40 845
BanI G'GyrC_C
Cuts at: 0 756 780 885
Size: 756 24 105
Fragments arranged by size:
756 105 24
BanII G_rGCy'C
Cuts at: 0 41 688 885
Size: 41 647 197
Fragments arranged by size:
647 197 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 416 885
Size: 416 469
BglI GCCn_nnn'nGGC
Cuts at: 0 436 885
Size: 436 449
BlpI GC'TnA_GC
Cuts at: 0 166 885
Size: 166 719
Bme1580I G_kGCm'C
Cuts at: 0 688 761 785 885
Size: 688 73 24 100
Fragments arranged by size:
688 100 73 24
BmgBI CAC'GTC
Cuts at: 0 726 885
Size: 726 159
BmrI ACTGGGnnnn_n'
Cuts at: 0 493 628 885
Size: 493 135 257
Fragments arranged by size:
493 257 135
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 191 885
Size: 191 694
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 885
Size: 622 263
BsaI GGTCTCn'nnnn_
Cuts at: 0 1 94 885
Size: 1 93 791
Fragments arranged by size:
791 93 1
BsaWI w'CCGG_w
Cuts at: 0 85 885
Size: 85 800
BseYI C'CCAG_C
Cuts at: 0 680 885
Size: 680 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 350 480 885
Size: 348 2 130 405
Fragments arranged by size:
405 348 130 2
BsiHKAI G_wGCw'C
Cuts at: 0 41 410 885
Size: 41 369 475
Fragments arranged by size:
475 369 41
BsmI GAATG_Cn'
Cuts at: 0 207 885
Size: 207 678
BsmBI CGTCTCn'nnnn_
Cuts at: 0 402 885
Size: 402 483
Bsp1286I G_dGCh'C
Cuts at: 0 41 410 688 761 785 885
Size: 41 369 278 73 24 100
Fragments arranged by size:
369 278 100 73 41 24
BsrDI GCAATG_nn'
Cuts at: 0 767 822 885
Size: 767 55 63
Fragments arranged by size:
767 63 55
BsrFI r'CCGG_y
Cuts at: 0 25 885
Size: 25 860
BstAPI GCAn_nnn'nTGC
Cuts at: 0 756 885
Size: 756 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 321 702 885
Size: 321 381 183
Fragments arranged by size:
381 321 183
BtsI GCAGTG_nn'
Cuts at: 0 333 850 885
Size: 333 517 35
Fragments arranged by size:
517 333 35
EaeI y'GGCC_r
Cuts at: 0 311 630 885
Size: 311 319 255
Fragments arranged by size:
319 311 255
EarI CTCTTCn'nnn_
Cuts at: 0 415 547 583 619 856 885
Size: 415 132 36 36 237 29
Fragments arranged by size:
415 237 132 36 36 29
EcoICRI GAG'CTC
Cuts at: 0 39 885
Size: 39 846
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 885
Size: 622 263
EcoO109I rG'GnC_Cy
Cuts at: 0 684 685 885
Size: 684 1 200
Fragments arranged by size:
684 200 1
HaeII r_GCGC'y
Cuts at: 0 252 885
Size: 252 633
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 618 650 885
Size: 618 32 235
Fragments arranged by size:
618 235 32
MscI TGG'CCA
Cuts at: 0 313 632 885
Size: 313 319 253
Fragments arranged by size:
319 313 253
MspA1I CmG'CkG
Cuts at: 0 378 885
Size: 378 507
NspI r_CATG'y
Cuts at: 0 494 885
Size: 494 391
PasI CC'CwG_GG
Cuts at: 0 810 885
Size: 810 75
PflMI CCAn_nnn'nTGG
Cuts at: 0 159 341 885
Size: 159 182 544
Fragments arranged by size:
544 182 159
PspOMI G'GGCC_C
Cuts at: 0 684 885
Size: 684 201
PspXI vC'TCGA_Gb
Cuts at: 0 40 885
Size: 40 845
PstI C_TGCA'G
Cuts at: 0 325 752 832 885
Size: 325 427 80 53
Fragments arranged by size:
427 325 80 53
PvuII CAG'CTG
Cuts at: 0 378 885
Size: 378 507
SacI G_AGCT'C
Cuts at: 0 41 885
Size: 41 844
SapI GCTCTTCn'nnn_
Cuts at: 0 415 885
Size: 415 470
SfcI C'TryA_G
Cuts at: 0 321 597 748 828 885
Size: 321 276 151 80 57
Fragments arranged by size:
321 276 151 80 57
SmlI C'TyrA_G
Cuts at: 0 40 885
Size: 40 845
StyI C'CwwG_G
Cuts at: 0 512 885
Size: 512 373
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 750 885
Size: 750 135
XhoI C'TCGA_G
Cuts at: 0 40 885
Size: 40 845
Enzymes that do cut:
AlwNI ApaI AvaI BanI BanII BbsI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI BsaI BsaWI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrFI BstAPI
BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII
Hin4I MscI MspA1I NspI PasI PflMI PspOMI PspXI
PstI PvuII SacI SapI SfcI SmlI StyI XcmI
XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BclI BfrBI BglII BmtI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaXI BseRI BsiEI BsiWI BspEI
BspHI BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XmaI XmnI ZraI