Restriction map for
pFN21AE9530
(Linear) (Six-base) MAPSORT of: KIEE9530.seq Check: 4577 from: 1 to: 1245
KIEE9530.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1083 1245
Size: 1083 162
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 237 855 1245
Size: 4 233 618 390
Fragments arranged by size:
618 390 233 4
AfeI AGC'GCT
Cuts at: 0 430 1245
Size: 430 815
ApaI G_GGCC'C
Cuts at: 0 347 489 846 1245
Size: 347 142 357 399
Fragments arranged by size:
399 357 347 142
BamHI G'GATC_C
Cuts at: 0 494 1245
Size: 494 751
BanI G'GyrC_C
Cuts at: 0 479 1245
Size: 479 766
BanII G_rGCy'C
Cuts at: 0 347 489 846 958 1245
Size: 347 142 357 112 287
Fragments arranged by size:
357 347 287 142 112
BbeI G_GCGC'C
Cuts at: 0 483 1245
Size: 483 762
BbsI GAAGACnn'nnnn_
Cuts at: 0 235 933 1245
Size: 235 698 312
Fragments arranged by size:
698 312 235
BbvCI CC'TCA_GC
Cuts at: 0 403 1245
Size: 403 842
BlpI GC'TnA_GC
Cuts at: 0 957 1245
Size: 957 288
Bme1580I G_kGCm'C
Cuts at: 0 347 489 846 1245
Size: 347 142 357 399
Fragments arranged by size:
399 357 347 142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 1245
Size: 322 923
Bpu10I CC'TnA_GC
Cuts at: 0 403 952 1245
Size: 403 549 293
Fragments arranged by size:
549 403 293
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1245
Size: 766 479
BsaI GGTCTCn'nnnn_
Cuts at: 0 270 373 1245
Size: 270 103 872
Fragments arranged by size:
872 270 103
BsaAI yAC'GTr
Cuts at: 0 435 1245
Size: 435 810
BsaHI Gr'CG_yC
Cuts at: 0 232 480 1245
Size: 232 248 765
Fragments arranged by size:
765 248 232
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 667 898 1245
Size: 103 564 231 347
Fragments arranged by size:
564 347 231 103
BseYI C'CCAG_C
Cuts at: 0 1052 1079 1245
Size: 1052 27 166
Fragments arranged by size:
1052 166 27
BsiHKAI G_wGCw'C
Cuts at: 0 475 527 958 1245
Size: 475 52 431 287
Fragments arranged by size:
475 431 287 52
BsmI GAATG_Cn'
Cuts at: 0 30 299 768 1009 1108 1245
Size: 30 269 469 241 99 137
Fragments arranged by size:
469 269 241 137 99 30
Bsp1286I G_dGCh'C
Cuts at: 0 347 475 489 527 846 958 1245
Size: 347 128 14 38 319 112 287
Fragments arranged by size:
347 319 287 128 112 38 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1083 1245
Size: 1083 162
BsrDI GCAATG_nn'
Cuts at: 0 192 1245
Size: 192 1053
BstEII G'GTnAC_C
Cuts at: 0 654 1245
Size: 654 591
BstYI r'GATC_y
Cuts at: 0 35 57 494 632 1245
Size: 35 22 437 138 613
Fragments arranged by size:
613 437 138 35 22
Bsu36I CC'TnA_GG
Cuts at: 0 490 1245
Size: 490 755
BtgI C'CryG_G
Cuts at: 0 347 552 1245
Size: 347 205 693
Fragments arranged by size:
693 347 205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 149 184 1245
Size: 149 35 1061
Fragments arranged by size:
1061 149 35
DraIII CAC_nnn'GTG
Cuts at: 0 1113 1245
Size: 1113 132
DrdI GACnn_nn'nnGTC
Cuts at: 0 277 1245
Size: 277 968
EaeI y'GGCC_r
Cuts at: 0 437 602 1245
Size: 437 165 643
Fragments arranged by size:
643 437 165
EarI CTCTTCn'nnn_
Cuts at: 0 256 532 871 1032 1245
Size: 256 276 339 161 213
Fragments arranged by size:
339 276 256 213 161
EcoICRI GAG'CTC
Cuts at: 0 956 1245
Size: 956 289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 237 322 855 1245
Size: 4 233 85 533 390
Fragments arranged by size:
533 390 233 85 4
EcoO109I rG'GnC_Cy
Cuts at: 0 486 584 843 1135 1245
Size: 486 98 259 292 110
Fragments arranged by size:
486 292 259 110 98
HaeII r_GCGC'y
Cuts at: 0 432 483 1245
Size: 432 51 762
Fragments arranged by size:
762 432 51
HincII GTy'rAC
Cuts at: 0 798 966 1245
Size: 798 168 279
Fragments arranged by size:
798 279 168
HindIII A'AGCT_T
Cuts at: 0 1224 1245
Size: 1224 21
KasI G'GCGC_C
Cuts at: 0 479 1245
Size: 479 766
MscI TGG'CCA
Cuts at: 0 439 1245
Size: 439 806
MspA1I CmG'CkG
Cuts at: 0 961 1083 1245
Size: 961 122 162
Fragments arranged by size:
961 162 122
NarI GG'CG_CC
Cuts at: 0 480 1245
Size: 480 765
NcoI C'CATG_G
Cuts at: 0 552 1245
Size: 552 693
PflMI CCAn_nnn'nTGG
Cuts at: 0 1113 1245
Size: 1113 132
PfoI T'CCnGG_A
Cuts at: 0 31 1245
Size: 31 1214
PpuMI rG'GwC_Cy
Cuts at: 0 584 1135 1245
Size: 584 551 110
Fragments arranged by size:
584 551 110
PspOMI G'GGCC_C
Cuts at: 0 343 485 842 1245
Size: 343 142 357 403
Fragments arranged by size:
403 357 343 142
PstI C_TGCA'G
Cuts at: 0 215 470 787 1245
Size: 215 255 317 458
Fragments arranged by size:
458 317 255 215
PvuII CAG'CTG
Cuts at: 0 961 1245
Size: 961 284
SacI G_AGCT'C
Cuts at: 0 958 1245
Size: 958 287
SanDI GG'GwC_CC
Cuts at: 0 584 1245
Size: 584 661
SapI GCTCTTCn'nnn_
Cuts at: 0 532 1245
Size: 532 713
SfcI C'TryA_G
Cuts at: 0 211 466 783 1245
Size: 211 255 317 462
Fragments arranged by size:
462 317 255 211
SfoI GGC'GCC
Cuts at: 0 481 1245
Size: 481 764
SmlI C'TyrA_G
Cuts at: 0 745 1245
Size: 745 500
SspI AAT'ATT
Cuts at: 0 771 1245
Size: 771 474
StyI C'CwwG_G
Cuts at: 0 288 552 1245
Size: 288 264 693
Fragments arranged by size:
693 288 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 875 1138 1245
Size: 875 263 107
Fragments arranged by size:
875 263 107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 420 585 1245
Size: 420 165 660
Fragments arranged by size:
660 420 165
XmnI GAAnn'nnTTC
Cuts at: 0 17 1090 1245
Size: 17 1073 155
Fragments arranged by size:
1073 155 17
Enzymes that do cut:
AarI AcuI AfeI ApaI BamHI BanI BanII BbeI
BbsI BbvCI BlpI Bme1580I BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BstEII BstYI Bsu36I BtgI CspCI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoO109I HaeII HincII HindIII
KasI MscI MspA1I NarI NcoI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI SanDI SapI SfcI SfoI
SmlI SspI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmrI BmtI BplI BsaBI BsaWI
BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I
BtgZI BtsI ClaI DraI EagI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HpaI KpnI
MfeI MluI MmeI MslI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmaI ZraI