Restriction map for
pFN21AE9529
(Linear) (Six-base) MAPSORT of: KIEE9529.seq Check: 190 from: 1 to: 1059
KIEE9529.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 478 1059
Size: 478 581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 939 1059
Size: 939 120
AfeI AGC'GCT
Cuts at: 0 749 1059
Size: 749 310
AleI CACnn'nnGTG
Cuts at: 0 472 763 1059
Size: 472 291 296
Fragments arranged by size:
472 296 291
AlwNI CAG_nnn'CTG
Cuts at: 0 244 1059
Size: 244 815
ApaI G_GGCC'C
Cuts at: 0 792 1059
Size: 792 267
ApaLI G'TGCA_C
Cuts at: 0 418 1059
Size: 418 641
ApoI r'AATT_y
Cuts at: 0 817 1059
Size: 817 242
AvaI C'yCGr_G
Cuts at: 0 461 1059
Size: 461 598
AvrII C'CTAG_G
Cuts at: 0 156 1059
Size: 156 903
BanII G_rGCy'C
Cuts at: 0 29 321 792 1059
Size: 29 292 471 267
Fragments arranged by size:
471 292 267 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 174 648 1059
Size: 174 474 411
Fragments arranged by size:
474 411 174
BglI GCCn_nnn'nGGC
Cuts at: 0 198 235 1059
Size: 198 37 824
Fragments arranged by size:
824 198 37
BglII A'GATC_T
Cuts at: 0 546 1059
Size: 546 513
Bme1580I G_kGCm'C
Cuts at: 0 422 792 1059
Size: 422 370 267
Fragments arranged by size:
422 370 267
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 340 1059
Size: 340 719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1059
Size: 71 988
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 1059
Size: 522 537
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 313 565 712 1059
Size: 313 252 147 347
Fragments arranged by size:
347 313 252 147
BseYI C'CCAG_C
Cuts at: 0 785 1059
Size: 785 274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 1059
Size: 91 968
BsiEI CG_ry'CG
Cuts at: 0 653 1059
Size: 653 406
BsiHKAI G_wGCw'C
Cuts at: 0 29 422 479 1059
Size: 29 393 57 580
Fragments arranged by size:
580 393 57 29
Bsp1286I G_dGCh'C
Cuts at: 0 29 321 422 479 792 1059
Size: 29 292 101 57 313 267
Fragments arranged by size:
313 292 267 101 57 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 110 478 1059
Size: 110 368 581
Fragments arranged by size:
581 368 110
BsrFI r'CCGG_y
Cuts at: 0 947 1059
Size: 947 112
BssSI C'ACGA_G
Cuts at: 0 763 1059
Size: 763 296
BstAPI GCAn_nnn'nTGC
Cuts at: 0 114 1059
Size: 114 945
BstEII G'GTnAC_C
Cuts at: 0 465 1059
Size: 465 594
BstXI CCAn_nnnn'nTGG
Cuts at: 0 95 1059
Size: 95 964
BstYI r'GATC_y
Cuts at: 0 546 1059
Size: 546 513
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 590 625 1059
Size: 590 35 434
Fragments arranged by size:
590 434 35
DraIII CAC_nnn'GTG
Cuts at: 0 114 1059
Size: 114 945
EaeI y'GGCC_r
Cuts at: 0 227 386 428 887 1059
Size: 227 159 42 459 172
Fragments arranged by size:
459 227 172 159 42
EarI CTCTTCn'nnn_
Cuts at: 0 58 958 1059
Size: 58 900 101
Fragments arranged by size:
900 101 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 465 1059
Size: 465 594
EcoICRI GAG'CTC
Cuts at: 0 27 1059
Size: 27 1032
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 939 1059
Size: 71 868 120
Fragments arranged by size:
868 120 71
EcoO109I rG'GnC_Cy
Cuts at: 0 99 495 789 1059
Size: 99 396 294 270
Fragments arranged by size:
396 294 270 99
EcoRI G'AATT_C
Cuts at: 0 817 1059
Size: 817 242
HaeII r_GCGC'y
Cuts at: 0 751 1059
Size: 751 308
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 1059
Size: 16 32 1011
Fragments arranged by size:
1011 32 16
MfeI C'AATT_G
Cuts at: 0 883 1059
Size: 883 176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 1059
Size: 62 997
MscI TGG'CCA
Cuts at: 0 229 388 430 889 1059
Size: 229 159 42 459 170
Fragments arranged by size:
459 229 170 159 42
MslI CAynn'nnrTG
Cuts at: 0 472 763 1059
Size: 472 291 296
Fragments arranged by size:
472 296 291
MspA1I CmG'CkG
Cuts at: 0 447 1059
Size: 447 612
NaeI GCC'GGC
Cuts at: 0 949 1059
Size: 949 110
NgoMIV G'CCGG_C
Cuts at: 0 947 1059
Size: 947 112
PflMI CCAn_nnn'nTGG
Cuts at: 0 675 968 1059
Size: 675 293 91
Fragments arranged by size:
675 293 91
PfoI T'CCnGG_A
Cuts at: 0 812 1059
Size: 812 247
PpuMI rG'GwC_Cy
Cuts at: 0 99 1059
Size: 99 960
PshAI GACnn'nnGTC
Cuts at: 0 964 1059
Size: 964 95
PsiI TTA'TAA
Cuts at: 0 980 1059
Size: 980 79
PspOMI G'GGCC_C
Cuts at: 0 788 1059
Size: 788 271
PvuI CG_AT'CG
Cuts at: 0 653 1059
Size: 653 406
SacI G_AGCT'C
Cuts at: 0 29 1059
Size: 29 1030
SapI GCTCTTCn'nnn_
Cuts at: 0 58 958 1059
Size: 58 900 101
Fragments arranged by size:
900 101 58
SmaI CCC'GGG
Cuts at: 0 463 1059
Size: 463 596
SmlI C'TyrA_G
Cuts at: 0 501 1059
Size: 501 558
SspI AAT'ATT
Cuts at: 0 621 1059
Size: 621 438
StuI AGG'CCT
Cuts at: 0 589 1059
Size: 589 470
StyI C'CwwG_G
Cuts at: 0 156 962 1059
Size: 156 806 97
Fragments arranged by size:
806 156 97
XbaI T'CTAG_A
Cuts at: 0 21 1059
Size: 21 1038
XmaI C'CCGG_G
Cuts at: 0 461 1059
Size: 461 598
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI ApaLI ApoI
AvaI AvrII BanII BbsI BglI BglII Bme1580I BplI
BpmI BpuEI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrFI BssSI BstAPI BstEII BstXI BstYI CspCI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
HaeII Hin4I MfeI MmeI MscI MslI MspA1I NaeI
NgoMIV PflMI PfoI PpuMI PshAI PsiI PspOMI PvuI
SacI SapI SmaI SmlI SspI StuI StyI XbaI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI AscI AseI AsiSI BaeI BamHI BanI
BbeI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI
BmrI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BstBI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EagI EcoNI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MluI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PspXI PsrI
PstI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SwaI TaqII TatI TstI Tth111I XcmI
XhoI XmnI ZraI