Restriction map for
pFN21AE9528
(Linear) (Six-base) MAPSORT of: KIEE9528.seq Check: 5601 from: 1 to: 1152
KIEE9528.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AccI GT'mk_AC
Cuts at: 0 296 1152
Size: 296 856
AflIII A'CryG_T
Cuts at: 0 587 1152
Size: 587 565
AlwNI CAG_nnn'CTG
Cuts at: 0 75 1152
Size: 75 1077
ApoI r'AATT_y
Cuts at: 0 3 1152
Size: 3 1149
AseI AT'TA_AT
Cuts at: 0 304 1152
Size: 304 848
AvaI C'yCGr_G
Cuts at: 0 434 1025 1152
Size: 434 591 127
Fragments arranged by size:
591 434 127
BclI T'GATC_A
Cuts at: 0 149 182 597 1152
Size: 149 33 415 555
Fragments arranged by size:
555 415 149 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 324 1099 1152
Size: 324 775 53
Fragments arranged by size:
775 324 53
Bpu10I CC'TnA_GC
Cuts at: 0 126 1152
Size: 126 1026
BsaI GGTCTCn'nnnn_
Cuts at: 0 438 1028 1152
Size: 438 590 124
Fragments arranged by size:
590 438 124
BsaAI yAC'GTr
Cuts at: 0 588 1152
Size: 588 564
BsaBI GATnn'nnATC
Cuts at: 0 98 187 863 1152
Size: 98 89 676 289
Fragments arranged by size:
676 289 98 89
BsaWI w'CCGG_w
Cuts at: 0 1019 1152
Size: 1019 133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 631 1152
Size: 631 521
BseYI C'CCAG_C
Cuts at: 0 68 1152
Size: 68 1084
BsmI GAATG_Cn'
Cuts at: 0 69 1152
Size: 69 1083
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 892 1152
Size: 892 260
BsrDI GCAATG_nn'
Cuts at: 0 364 398 875 932 1152
Size: 364 34 477 57 220
Fragments arranged by size:
477 364 220 57 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 346 1152
Size: 346 806
DraI TTT'AAA
Cuts at: 0 1116 1152
Size: 1116 36
DrdI GACnn_nn'nnGTC
Cuts at: 0 316 1152
Size: 316 836
EaeI y'GGCC_r
Cuts at: 0 110 1152
Size: 110 1042
EcoO109I rG'GnC_Cy
Cuts at: 0 489 1152
Size: 489 663
EcoRI G'AATT_C
Cuts at: 0 3 1152
Size: 3 1149
EcoRV GAT'ATC
Cuts at: 0 932 1152
Size: 932 220
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 134 166 1006 1038 1152
Size: 134 32 840 32 114
Fragments arranged by size:
840 134 114 32 32
HincII GTy'rAC
Cuts at: 0 945 1152
Size: 945 207
MscI TGG'CCA
Cuts at: 0 112 1152
Size: 112 1040
MslI CAynn'nnrTG
Cuts at: 0 304 403 464 925 1070 1152
Size: 304 99 61 461 145 82
Fragments arranged by size:
461 304 145 99 82 61
MspA1I CmG'CkG
Cuts at: 0 420 1152
Size: 420 732
NspI r_CATG'y
Cuts at: 0 463 1152
Size: 463 689
PflMI CCAn_nnn'nTGG
Cuts at: 0 75 1152
Size: 75 1077
PpuMI rG'GwC_Cy
Cuts at: 0 489 1152
Size: 489 663
PspXI vC'TCGA_Gb
Cuts at: 0 434 1152
Size: 434 718
PstI C_TGCA'G
Cuts at: 0 656 1152
Size: 656 496
PvuII CAG'CTG
Cuts at: 0 420 1152
Size: 420 732
SfcI C'TryA_G
Cuts at: 0 652 1152
Size: 652 500
SmlI C'TyrA_G
Cuts at: 0 434 1152
Size: 434 718
StyI C'CwwG_G
Cuts at: 0 107 1152
Size: 107 1045
TatI w'GTAC_w
Cuts at: 0 755 1152
Size: 755 397
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 319 387 642 1152
Size: 319 68 255 510
Fragments arranged by size:
510 319 255 68
XhoI C'TCGA_G
Cuts at: 0 434 1152
Size: 434 718
Enzymes that do cut:
AccI AflIII AlwNI ApoI AseI AvaI BclI BmrI
Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BseYI BsmI
BspMI BsrDI BtgZI DraI DrdI EaeI EcoO109I EcoRI
EcoRV Hin4I HincII MscI MslI MspA1I NspI PflMI
PpuMI PspXI PstI PvuII SfcI SmlI StyI TatI
XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI BpmI BpuEI BsaHI BsaXI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraIII EagI
EarI EciI EcoICRI Eco57MI EcoNI FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XmaI
XmnI ZraI