Restriction map for
pFN21AE9526
(Linear) (Six-base) MAPSORT of: KIEE9526.seq Check: 4289 from: 1 to: 1314
KIEE9526.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 435 1177 1314
Size: 219 216 742 137
Fragments arranged by size:
742 219 216 137
AgeI A'CCGG_T
Cuts at: 0 805 1314
Size: 805 509
AhdI GACnn_n'nnGTC
Cuts at: 0 291 1314
Size: 291 1023
AlwNI CAG_nnn'CTG
Cuts at: 0 1071 1104 1214 1314
Size: 1071 33 110 100
Fragments arranged by size:
1071 110 100 33
ApoI r'AATT_y
Cuts at: 0 911 1314
Size: 911 403
AvaI C'yCGr_G
Cuts at: 0 1087 1314
Size: 1087 227
BanI G'GyrC_C
Cuts at: 0 216 1178 1249 1314
Size: 216 962 71 65
Fragments arranged by size:
962 216 71 65
BanII G_rGCy'C
Cuts at: 0 674 1314
Size: 674 640
BbeI G_GCGC'C
Cuts at: 0 1253 1314
Size: 1253 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 225 612 806 1314
Size: 225 387 194 508
Fragments arranged by size:
508 387 225 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1160 1194 1314
Size: 1160 34 120
Fragments arranged by size:
1160 120 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 810 1314
Size: 810 504
BlpI GC'TnA_GC
Cuts at: 0 610 1314
Size: 610 704
Bme1580I G_kGCm'C
Cuts at: 0 221 839 1183 1314
Size: 221 618 344 131
Fragments arranged by size:
618 344 221 131
BmrI ACTGGGnnnn_n'
Cuts at: 0 1275 1314
Size: 1275 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 1314
Size: 537 777
BsaI GGTCTCn'nnnn_
Cuts at: 0 134 199 1294 1314
Size: 134 65 1095 20
Fragments arranged by size:
1095 134 65 20
BsaAI yAC'GTr
Cuts at: 0 179 1314
Size: 179 1135
BsaHI Gr'CG_yC
Cuts at: 0 161 1013 1250 1314
Size: 161 852 237 64
Fragments arranged by size:
852 237 161 64
BsaWI w'CCGG_w
Cuts at: 0 805 1314
Size: 805 509
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 194 224 1255 1285 1314
Size: 194 30 1031 30 29
Fragments arranged by size:
1031 194 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1044 1314
Size: 1044 270
BseYI C'CCAG_C
Cuts at: 0 812 1314
Size: 812 502
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 445 450 1165 1314
Size: 134 311 5 715 149
Fragments arranged by size:
715 311 149 134 5
BsmI GAATG_Cn'
Cuts at: 0 59 76 1314
Size: 59 17 1238
Fragments arranged by size:
1238 59 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 292 1314
Size: 292 1022
Bsp1286I G_dGCh'C
Cuts at: 0 221 674 839 1183 1314
Size: 221 453 165 344 131
Fragments arranged by size:
453 344 221 165 131
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 40 1314
Size: 40 1274
BsrFI r'CCGG_y
Cuts at: 0 805 1175 1314
Size: 805 370 139
Fragments arranged by size:
805 370 139
BstAPI GCAn_nnn'nTGC
Cuts at: 0 778 1314
Size: 778 536
BstXI CCAn_nnnn'nTGG
Cuts at: 0 593 1314
Size: 593 721
BtgI C'CryG_G
Cuts at: 0 1266 1314
Size: 1266 48
BtsI GCAGTG_nn'
Cuts at: 0 162 462 570 1153 1314
Size: 162 300 108 583 161
Fragments arranged by size:
583 300 162 161 108
DraIII CAC_nnn'GTG
Cuts at: 0 834 1314
Size: 834 480
EaeI y'GGCC_r
Cuts at: 0 641 695 782 977 1112 1314
Size: 641 54 87 195 135 202
Fragments arranged by size:
641 202 195 135 87 54
EarI CTCTTCn'nnn_
Cuts at: 0 454 1314
Size: 454 860
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 435 537 1177 1314
Size: 219 216 102 640 137
Fragments arranged by size:
640 219 216 137 102
EcoO109I rG'GnC_Cy
Cuts at: 0 747 1101 1314
Size: 747 354 213
Fragments arranged by size:
747 354 213
HaeII r_GCGC'y
Cuts at: 0 1253 1314
Size: 1253 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 194 226 277 309 571 603 734 766
Size: 194 32 51 32 262 32 131 32
Cuts at: 766 1314
Size: 548
Fragments arranged by size:
548 262 194 131 51 32 32 32
32
HincII GTy'rAC
Cuts at: 0 929 1314
Size: 929 385
HpaI GTT'AAC
Cuts at: 0 929 1314
Size: 929 385
KasI G'GCGC_C
Cuts at: 0 1249 1314
Size: 1249 65
MscI TGG'CCA
Cuts at: 0 643 697 979 1314
Size: 643 54 282 335
Fragments arranged by size:
643 335 282 54
MslI CAynn'nnrTG
Cuts at: 0 523 1314
Size: 523 791
MspA1I CmG'CkG
Cuts at: 0 1005 1268 1314
Size: 1005 263 46
Fragments arranged by size:
1005 263 46
NarI GG'CG_CC
Cuts at: 0 1250 1314
Size: 1250 64
PasI CC'CwG_GG
Cuts at: 0 393 1314
Size: 393 921
PflMI CCAn_nnn'nTGG
Cuts at: 0 1023 1104 1314
Size: 1023 81 210
Fragments arranged by size:
1023 210 81
PmlI CAC'GTG
Cuts at: 0 179 1314
Size: 179 1135
PshAI GACnn'nnGTC
Cuts at: 0 1306 1314
Size: 1306 8
PstI C_TGCA'G
Cuts at: 0 157 629 923 1259 1314
Size: 157 472 294 336 55
Fragments arranged by size:
472 336 294 157 55
SacII CC_GC'GG
Cuts at: 0 1269 1314
Size: 1269 45
ScaI AGT'ACT
Cuts at: 0 631 1314
Size: 631 683
SfcI C'TryA_G
Cuts at: 0 153 330 399 625 919 1255 1314
Size: 153 177 69 226 294 336 59
Fragments arranged by size:
336 294 226 177 153 69 59
SfoI GGC'GCC
Cuts at: 0 1251 1314
Size: 1251 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 80 585 1296 1314
Size: 80 505 711 18
Fragments arranged by size:
711 505 80 18
TatI w'GTAC_w
Cuts at: 0 629 965 1314
Size: 629 336 349
Fragments arranged by size:
629 349 336
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1255 1287 1314
Size: 1255 32 27
Fragments arranged by size:
1255 32 27
Tth111I GACn'n_nGTC
Cuts at: 0 1188 1314
Size: 1188 126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 390 525 1314
Size: 390 135 789
Fragments arranged by size:
789 390 135
XmnI GAAnn'nnTTC
Cuts at: 0 493 1314
Size: 493 821
Enzymes that do cut:
AcuI AgeI AhdI AlwNI ApoI AvaI BanI BanII
BbeI BbsI BcgI BciVI BlpI Bme1580I BmrI BpmI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsmI BsmBI Bsp1286I BspMI BsrFI BstAPI BstXI BtgI
BtsI DraIII EaeI EarI Eco57MI EcoO109I HaeII Hin4I
HincII HpaI KasI MscI MslI MspA1I NarI PasI
PflMI PmlI PshAI PstI SacII ScaI SfcI SfoI
TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BclI BfrBI BglI BglII
BmgBI BmtI BplI Bpu10I BpuEI BsaBI BsiEI BsiHKAI
BsiWI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII KpnI MfeI MluI
MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PpiI PpuMI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI XbaI XhoI XmaI ZraI