Restriction map for
pFN21AE9524
(Linear) (Six-base) MAPSORT of: KIEE9524.seq Check: 4889 from: 1 to: 1044
KIEE9524.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AccI GT'mk_AC
Cuts at: 0 1024 1044
Size: 1024 20
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 417 1044
Size: 32 385 627
Fragments arranged by size:
627 385 32
AfeI AGC'GCT
Cuts at: 0 394 1044
Size: 394 650
AflIII A'CryG_T
Cuts at: 0 224 1044
Size: 224 820
AlwNI CAG_nnn'CTG
Cuts at: 0 669 1044
Size: 669 375
ApaI G_GGCC'C
Cuts at: 0 678 1044
Size: 678 366
BamHI G'GATC_C
Cuts at: 0 766 1044
Size: 766 278
BanI G'GyrC_C
Cuts at: 0 574 1044
Size: 574 470
BanII G_rGCy'C
Cuts at: 0 678 1044
Size: 678 366
BbsI GAAGACnn'nnnn_
Cuts at: 0 123 255 264 294 1044
Size: 123 132 9 30 750
Fragments arranged by size:
750 132 123 30 9
BbvCI CC'TCA_GC
Cuts at: 0 670 1044
Size: 670 374
BciVI GTATCCnnnnn_n'
Cuts at: 0 286 1044
Size: 286 758
BglI GCCn_nnn'nGGC
Cuts at: 0 382 1044
Size: 382 662
BlpI GC'TnA_GC
Cuts at: 0 695 1044
Size: 695 349
Bme1580I G_kGCm'C
Cuts at: 0 127 343 577 678 791 1044
Size: 127 216 234 101 113 253
Fragments arranged by size:
253 234 216 127 113 101
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 259 1044
Size: 259 785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 534 1044
Size: 91 443 510
Fragments arranged by size:
510 443 91
Bpu10I CC'TnA_GC
Cuts at: 0 670 1044
Size: 670 374
BsaAI yAC'GTr
Cuts at: 0 602 786 1044
Size: 602 184 258
Fragments arranged by size:
602 258 184
BsaHI Gr'CG_yC
Cuts at: 0 488 1044
Size: 488 556
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 7 37 1044
Size: 7 30 1007
Fragments arranged by size:
1007 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 1044
Size: 235 809
BsiEI CG_ry'CG
Cuts at: 0 309 1044
Size: 309 735
BsmBI CGTCTCn'nnnn_
Cuts at: 0 495 1044
Size: 495 549
Bsp1286I G_dGCh'C
Cuts at: 0 127 343 577 678 791 1044
Size: 127 216 234 101 113 253
Fragments arranged by size:
253 234 216 127 113 101
BspHI T'CATG_A
Cuts at: 0 914 941 1044
Size: 914 27 103
Fragments arranged by size:
914 103 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 340 1044
Size: 340 704
BsrDI GCAATG_nn'
Cuts at: 0 80 1044
Size: 80 964
BsrFI r'CCGG_y
Cuts at: 0 518 1044
Size: 518 526
BsrGI T'GTAC_A
Cuts at: 0 809 1044
Size: 809 235
BstXI CCAn_nnnn'nTGG
Cuts at: 0 165 813 1044
Size: 165 648 231
Fragments arranged by size:
648 231 165
BstYI r'GATC_y
Cuts at: 0 766 1044
Size: 766 278
Bsu36I CC'TnA_GG
Cuts at: 0 530 1044
Size: 530 514
BtgI C'CryG_G
Cuts at: 0 58 158 569 831 1044
Size: 58 100 411 262 213
Fragments arranged by size:
411 262 213 100 58
BtsI GCAGTG_nn'
Cuts at: 0 175 547 1044
Size: 175 372 497
Fragments arranged by size:
497 372 175
EaeI y'GGCC_r
Cuts at: 0 170 1044
Size: 170 874
EarI CTCTTCn'nnn_
Cuts at: 0 14 51 616 1044
Size: 14 37 565 428
Fragments arranged by size:
565 428 37 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 91 417 534 1044
Size: 32 59 326 117 510
Fragments arranged by size:
510 326 117 59 32
EcoO109I rG'GnC_Cy
Cuts at: 0 534 674 675 1044
Size: 534 140 1 369
Fragments arranged by size:
534 369 140 1
HaeII r_GCGC'y
Cuts at: 0 396 1044
Size: 396 648
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 284 316 1044
Size: 284 32 728
Fragments arranged by size:
728 284 32
HincII GTy'rAC
Cuts at: 0 222 819 1044
Size: 222 597 225
Fragments arranged by size:
597 225 222
HindIII A'AGCT_T
Cuts at: 0 932 1044
Size: 932 112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 1044
Size: 1004 40
MscI TGG'CCA
Cuts at: 0 172 1044
Size: 172 872
MslI CAynn'nnrTG
Cuts at: 0 317 811 946 1044
Size: 317 494 135 98
Fragments arranged by size:
494 317 135 98
MspA1I CmG'CkG
Cuts at: 0 284 666 1044
Size: 284 382 378
Fragments arranged by size:
382 378 284
NcoI C'CATG_G
Cuts at: 0 158 831 1044
Size: 158 673 213
Fragments arranged by size:
673 213 158
NspI r_CATG'y
Cuts at: 0 448 989 1044
Size: 448 541 55
Fragments arranged by size:
541 448 55
PflMI CCAn_nnn'nTGG
Cuts at: 0 837 1044
Size: 837 207
PshAI GACnn'nnGTC
Cuts at: 0 457 1044
Size: 457 587
PspOMI G'GGCC_C
Cuts at: 0 674 1044
Size: 674 370
PstI C_TGCA'G
Cuts at: 0 542 1044
Size: 542 502
PvuII CAG'CTG
Cuts at: 0 284 666 1044
Size: 284 382 378
Fragments arranged by size:
382 378 284
SfcI C'TryA_G
Cuts at: 0 344 538 1044
Size: 344 194 506
Fragments arranged by size:
506 344 194
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 382 1044
Size: 382 662
SphI G_CATG'C
Cuts at: 0 448 1044
Size: 448 596
StyI C'CwwG_G
Cuts at: 0 158 831 1044
Size: 158 673 213
Fragments arranged by size:
673 213 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 295 1044
Size: 295 749
TatI w'GTAC_w
Cuts at: 0 809 1044
Size: 809 235
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 362 394 1044
Size: 362 32 650
Fragments arranged by size:
650 362 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 165 1044
Size: 165 879
Enzymes that do cut:
AccI AcuI AfeI AflIII AlwNI ApaI BamHI BanI
BanII BbsI BbvCI BciVI BglI BlpI Bme1580I BplI
BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BsiEI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstXI BstYI
Bsu36I BtgI BtsI EaeI EarI Eco57MI EcoO109I HaeII
Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI
NspI PflMI PshAI PspOMI PstI PvuII SfcI SfiI
SphI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BcgI BclI BfrBI BglII BmgBI
BmrI BmtI BpuEI BsaI BsaBI BsaWI BseYI BsgI
BsiHKAI BsiWI BsmI BspEI BsrBI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI