Restriction map for
pFN21AE9516
(Linear) (Six-base) MAPSORT of: KIEE9516.seq Check: 8356 from: 1 to: 2226
KIEE9516.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1300 1478 2226
Size: 1300 178 748
Fragments arranged by size:
1300 748 178
AleI CACnn'nnGTG
Cuts at: 0 1375 2226
Size: 1375 851
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 961 993 2226
Size: 961 32 1233
Fragments arranged by size:
1233 961 32
AlwNI CAG_nnn'CTG
Cuts at: 0 74 573 1197 1266 1668 1769 2226
Size: 74 499 624 69 402 101 457
Fragments arranged by size:
624 499 457 402 101 74 69
ApaI G_GGCC'C
Cuts at: 0 2202 2226
Size: 2202 24
ApoI r'AATT_y
Cuts at: 0 232 2155 2226
Size: 232 1923 71
Fragments arranged by size:
1923 232 71
AvaI C'yCGr_G
Cuts at: 0 282 2195 2226
Size: 282 1913 31
Fragments arranged by size:
1913 282 31
BamHI G'GATC_C
Cuts at: 0 737 897 2226
Size: 737 160 1329
Fragments arranged by size:
1329 737 160
BanI G'GyrC_C
Cuts at: 0 86 675 689 2056 2226
Size: 86 589 14 1367 170
Fragments arranged by size:
1367 589 170 86 14
BanII G_rGCy'C
Cuts at: 0 1453 1501 1631 2202 2226
Size: 1453 48 130 571 24
Fragments arranged by size:
1453 571 130 48 24
BbeI G_GCGC'C
Cuts at: 0 679 2060 2226
Size: 679 1381 166
Fragments arranged by size:
1381 679 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 194 228 2226
Size: 194 34 1998
Fragments arranged by size:
1998 194 34
BclI T'GATC_A
Cuts at: 0 1367 2226
Size: 1367 859
BglI GCCn_nnn'nGGC
Cuts at: 0 437 1619 1694 2054 2206 2226
Size: 437 1182 75 360 152 20
Fragments arranged by size:
1182 437 360 152 75 20
BlpI GC'TnA_GC
Cuts at: 0 597 2226
Size: 597 1629
Bme1580I G_kGCm'C
Cuts at: 0 81 91 1241 1709 2202 2226
Size: 81 10 1150 468 493 24
Fragments arranged by size:
1150 493 468 81 24 10
BmgBI CAC'GTC
Cuts at: 0 1173 1344 2171 2226
Size: 1173 171 827 55
Fragments arranged by size:
1173 827 171 55
BmrI ACTGGGnnnn_n'
Cuts at: 0 1251 2226
Size: 1251 975
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 378 2226
Size: 378 1848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 545 677 908 1018 1645 1821 2226
Size: 51 494 132 231 110 627 176 405
Fragments arranged by size:
627 494 405 231 176 132 110 51
Bpu10I CC'TnA_GC
Cuts at: 0 1309 2226
Size: 1309 917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1147 2226
Size: 1147 1079
BsaI GGTCTCn'nnnn_
Cuts at: 0 1148 2226
Size: 1148 1078
BsaHI Gr'CG_yC
Cuts at: 0 676 1041 1724 2057 2226
Size: 676 365 683 333 169
Fragments arranged by size:
683 676 365 333 169
BsaWI w'CCGG_w
Cuts at: 0 2111 2226
Size: 2111 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 26 2226
Size: 26 2200
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 90 1015 2226
Size: 90 925 1211
Fragments arranged by size:
1211 925 90
BseYI C'CCAG_C
Cuts at: 0 704 1266 2134 2226
Size: 704 562 868 92
Fragments arranged by size:
868 704 562 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 378 1456 1561 1956 2059 2226
Size: 297 81 1078 105 395 103 167
Fragments arranged by size:
1078 395 297 167 105 103 81
BsiEI CG_ry'CG
Cuts at: 0 1758 2007 2226
Size: 1758 249 219
Fragments arranged by size:
1758 249 219
BsiHKAI G_wGCw'C
Cuts at: 0 1631 2226
Size: 1631 595
BsmI GAATG_Cn'
Cuts at: 0 22 975 2226
Size: 22 953 1251
Fragments arranged by size:
1251 953 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 653 1731 2226
Size: 653 1078 495
Fragments arranged by size:
1078 653 495
Bsp1286I G_dGCh'C
Cuts at: 0 81 91 1241 1453 1501 1631 1709 2202
Size: 81 10 1150 212 48 130 78 493
Cuts at: 2202 2226
Size: 24
Fragments arranged by size:
1150 493 212 130 81 78 48 24
10
BspHI T'CATG_A
Cuts at: 0 731 2226
Size: 731 1495
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 980 1300 1478 2078 2226
Size: 980 320 178 600 148
Fragments arranged by size:
980 600 320 178 148
BsrBI CCG'CTC
Cuts at: 0 819 2226
Size: 819 1407
BsrDI GCAATG_nn'
Cuts at: 0 946 1845 2226
Size: 946 899 381
Fragments arranged by size:
946 899 381
BsrFI r'CCGG_y
Cuts at: 0 1516 2226
Size: 1516 710
BssSI C'ACGA_G
Cuts at: 0 102 2226
Size: 102 2124
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1197 1292 2082 2226
Size: 1197 95 790 144
Fragments arranged by size:
1197 790 144 95
BstBI TT'CG_AA
Cuts at: 0 787 2226
Size: 787 1439
BstEII G'GTnAC_C
Cuts at: 0 606 2226
Size: 606 1620
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1512 2226
Size: 1512 714
BstYI r'GATC_y
Cuts at: 0 737 897 2226
Size: 737 160 1329
Fragments arranged by size:
1329 737 160
Bsu36I CC'TnA_GG
Cuts at: 0 2203 2226
Size: 2203 23
BtgI C'CryG_G
Cuts at: 0 407 911 1023 1186 2226
Size: 407 504 112 163 1040
Fragments arranged by size:
1040 504 407 163 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1932 2226
Size: 1932 294
BtsI GCAGTG_nn'
Cuts at: 0 2076 2226
Size: 2076 150
DrdI GACnn_nn'nnGTC
Cuts at: 0 832 2226
Size: 832 1394
EaeI y'GGCC_r
Cuts at: 0 743 953 1514 1536 1565 1592 1755 1943
Size: 743 210 561 22 29 27 163 188
Cuts at: 1943 2181 2226
Size: 238 45
Fragments arranged by size:
743 561 238 210 188 163 45 29
27 22
EagI C'GGCC_G
Cuts at: 0 1755 2226
Size: 1755 471
EarI CTCTTCn'nnn_
Cuts at: 0 1146 1210 1402 1495 1963 2226
Size: 1146 64 192 93 468 263
Fragments arranged by size:
1146 468 263 192 93 64
EcoICRI GAG'CTC
Cuts at: 0 1629 2226
Size: 1629 597
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 545 677 908 1018 1645 1821 2226
Size: 51 494 132 231 110 627 176 405
Fragments arranged by size:
627 494 405 231 176 132 110 51
EcoO109I rG'GnC_Cy
Cuts at: 0 570 669 763 904 1665 1813 2199 2226
Size: 570 99 94 141 761 148 386 27
Fragments arranged by size:
761 570 386 148 141 99 94 27
EcoRI G'AATT_C
Cuts at: 0 2155 2226
Size: 2155 71
EcoRV GAT'ATC
Cuts at: 0 729 2226
Size: 729 1497
HaeII r_GCGC'y
Cuts at: 0 679 1390 1933 2060 2226
Size: 679 711 543 127 166
Fragments arranged by size:
711 679 543 166 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 443 475 636 668 2226
Size: 28 415 32 161 32 1558
Fragments arranged by size:
1558 415 161 32 32 28
HincII GTy'rAC
Cuts at: 0 1056 2226
Size: 1056 1170
KasI G'GCGC_C
Cuts at: 0 675 2056 2226
Size: 675 1381 170
Fragments arranged by size:
1381 675 170
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1056 1757 2226
Size: 1056 701 469
Fragments arranged by size:
1056 701 469
MscI TGG'CCA
Cuts at: 0 745 955 2183 2226
Size: 745 210 1228 43
Fragments arranged by size:
1228 745 210 43
MslI CAynn'nnrTG
Cuts at: 0 377 1375 1852 2226
Size: 377 998 477 374
Fragments arranged by size:
998 477 377 374
MspA1I CmG'CkG
Cuts at: 0 422 428 708 886 2226
Size: 422 6 280 178 1340
Fragments arranged by size:
1340 422 280 178 6
NaeI GCC'GGC
Cuts at: 0 1518 2226
Size: 1518 708
NarI GG'CG_CC
Cuts at: 0 676 2057 2226
Size: 676 1381 169
Fragments arranged by size:
1381 676 169
NgoMIV G'CCGG_C
Cuts at: 0 1516 2226
Size: 1516 710
PflMI CCAn_nnn'nTGG
Cuts at: 0 573 1266 2226
Size: 573 693 960
Fragments arranged by size:
960 693 573
PfoI T'CCnGG_A
Cuts at: 0 566 654 812 2226
Size: 566 88 158 1414
Fragments arranged by size:
1414 566 158 88
PpuMI rG'GwC_Cy
Cuts at: 0 570 1813 2226
Size: 570 1243 413
Fragments arranged by size:
1243 570 413
PshAI GACnn'nnGTC
Cuts at: 0 1861 2226
Size: 1861 365
PspOMI G'GGCC_C
Cuts at: 0 2198 2226
Size: 2198 28
PstI C_TGCA'G
Cuts at: 0 2054 2226
Size: 2054 172
PvuI CG_AT'CG
Cuts at: 0 2007 2226
Size: 2007 219
PvuII CAG'CTG
Cuts at: 0 422 708 886 2226
Size: 422 286 178 1340
Fragments arranged by size:
1340 422 286 178
SacI G_AGCT'C
Cuts at: 0 1631 2226
Size: 1631 595
SapI GCTCTTCn'nnn_
Cuts at: 0 1963 2226
Size: 1963 263
SbfI CC_TGCA'GG
Cuts at: 0 2054 2226
Size: 2054 172
ScaI AGT'ACT
Cuts at: 0 2149 2226
Size: 2149 77
SfcI C'TryA_G
Cuts at: 0 1740 2050 2226
Size: 1740 310 176
Fragments arranged by size:
1740 310 176
SfoI GGC'GCC
Cuts at: 0 677 2058 2226
Size: 677 1381 168
Fragments arranged by size:
1381 677 168
SmaI CCC'GGG
Cuts at: 0 284 2197 2226
Size: 284 1913 29
Fragments arranged by size:
1913 284 29
SmlI C'TyrA_G
Cuts at: 0 1126 2226
Size: 1126 1100
StuI AGG'CCT
Cuts at: 0 1622 2226
Size: 1622 604
StyI C'CwwG_G
Cuts at: 0 1085 1166 1656 2226
Size: 1085 81 490 570
Fragments arranged by size:
1085 570 490 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 718 2226
Size: 718 1508
TatI w'GTAC_w
Cuts at: 0 2147 2226
Size: 2147 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1114 1146 2226
Size: 1114 32 1080
Fragments arranged by size:
1114 1080 32
Tth111I GACn'n_nGTC
Cuts at: 0 481 562 2226
Size: 481 81 1664
Fragments arranged by size:
1664 481 81
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1380 1509 2226
Size: 1380 129 717
Fragments arranged by size:
1380 717 129
XmaI C'CCGG_G
Cuts at: 0 282 2195 2226
Size: 282 1913 31
Fragments arranged by size:
1913 282 31
Enzymes that do cut:
AarI AleI AloI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbeI BcgI BclI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV PflMI PfoI PpuMI
PshAI PspOMI PstI PvuI PvuII SacI SapI SbfI
ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI ApaLI AscI AseI AsiSI AvrII
BaeI BbsI BbvCI BciVI BfrBI BglII BmtI BsaAI
BsaBI BsiWI BspEI BsrGI BssHII BstZ17I ClaI CspCI
DraI DraIII EciI EcoNI FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PsiI PspXI PsrI RsrII SacII SalI
SanDI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmnI ZraI