Restriction map for
pFN21AE9515
(Linear) (Six-base) MAPSORT of: KIEE9515.seq Check: 8072 from: 1 to: 1110
KIEE9515.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1110
Size: 196 914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 253 532 1110
Size: 253 279 578
Fragments arranged by size:
578 279 253
AlwNI CAG_nnn'CTG
Cuts at: 0 156 1110
Size: 156 954
ApaI G_GGCC'C
Cuts at: 0 25 1110
Size: 25 1085
ApaLI G'TGCA_C
Cuts at: 0 327 490 586 1110
Size: 327 163 96 524
Fragments arranged by size:
524 327 163 96
AvaI C'yCGr_G
Cuts at: 0 17 1110
Size: 17 1093
BanI G'GyrC_C
Cuts at: 0 768 815 1110
Size: 768 47 295
Fragments arranged by size:
768 295 47
BanII G_rGCy'C
Cuts at: 0 25 608 649 1110
Size: 25 583 41 461
Fragments arranged by size:
583 461 41 25
BbeI G_GCGC'C
Cuts at: 0 819 1110
Size: 819 291
BbsI GAAGACnn'nnnn_
Cuts at: 0 402 879 1110
Size: 402 477 231
Fragments arranged by size:
477 402 231
BbvCI CC'TCA_GC
Cuts at: 0 499 523 1110
Size: 499 24 587
Fragments arranged by size:
587 499 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 77 111 1110
Size: 77 34 999
Fragments arranged by size:
999 77 34
BglI GCCn_nnn'nGGC
Cuts at: 0 550 1110
Size: 550 560
Bme1580I G_kGCm'C
Cuts at: 0 25 331 494 590 1110
Size: 25 306 163 96 520
Fragments arranged by size:
520 306 163 96 25
BmgBI CAC'GTC
Cuts at: 0 318 621 885 1110
Size: 318 303 264 225
Fragments arranged by size:
318 303 264 225
BmrI ACTGGGnnnn_n'
Cuts at: 0 810 1110
Size: 810 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 1110
Size: 116 994
Bpu10I CC'TnA_GC
Cuts at: 0 499 523 1110
Size: 499 24 587
Fragments arranged by size:
587 499 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1110
Size: 592 518
BsaAI yAC'GTr
Cuts at: 0 600 1110
Size: 600 510
BsaHI Gr'CG_yC
Cuts at: 0 816 1110
Size: 816 294
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 649 1045 1110
Size: 649 396 65
Fragments arranged by size:
649 396 65
BseYI C'CCAG_C
Cuts at: 0 377 1110
Size: 377 733
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 865 967 997 1110
Size: 256 609 102 30 113
Fragments arranged by size:
609 256 113 102 30
BsiHKAI G_wGCw'C
Cuts at: 0 323 331 494 590 608 1110
Size: 323 8 163 96 18 502
Fragments arranged by size:
502 323 163 96 18 8
Bsp1286I G_dGCh'C
Cuts at: 0 25 323 331 494 590 608 649 1110
Size: 25 298 8 163 96 18 41 461
Fragments arranged by size:
461 298 163 96 41 25 18 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 710 902 1110
Size: 710 192 208
Fragments arranged by size:
710 208 192
BsrBI CCG'CTC
Cuts at: 0 345 1110
Size: 345 765
BsrFI r'CCGG_y
Cuts at: 0 57 266 423 818 1110
Size: 57 209 157 395 292
Fragments arranged by size:
395 292 209 157 57
BstAPI GCAn_nnn'nTGC
Cuts at: 0 165 335 621 830 1110
Size: 165 170 286 209 280
Fragments arranged by size:
286 280 209 170 165
BstEII G'GTnAC_C
Cuts at: 0 426 693 1110
Size: 426 267 417
Fragments arranged by size:
426 417 267
BstXI CCAn_nnnn'nTGG
Cuts at: 0 377 468 1110
Size: 377 91 642
Fragments arranged by size:
642 377 91
BtgI C'CryG_G
Cuts at: 0 560 1077 1110
Size: 560 517 33
Fragments arranged by size:
560 517 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 447 1110
Size: 447 663
BtsI GCAGTG_nn'
Cuts at: 0 473 1110
Size: 473 637
DraIII CAC_nnn'GTG
Cuts at: 0 279 621 1110
Size: 279 342 489
Fragments arranged by size:
489 342 279
DrdI GACnn_nn'nnGTC
Cuts at: 0 478 1110
Size: 478 632
EaeI y'GGCC_r
Cuts at: 0 542 1080 1110
Size: 542 538 30
Fragments arranged by size:
542 538 30
EcoICRI GAG'CTC
Cuts at: 0 606 1110
Size: 606 504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 196 1110
Size: 116 80 914
Fragments arranged by size:
914 116 80
EcoNI CCTnn'n_nnAGG
Cuts at: 0 631 1110
Size: 631 479
EcoO109I rG'GnC_Cy
Cuts at: 0 21 22 903 1110
Size: 21 1 881 207
Fragments arranged by size:
881 207 21 1
FspI TGC'GCA
Cuts at: 0 1039 1110
Size: 1039 71
HaeII r_GCGC'y
Cuts at: 0 530 556 574 660 666 819 1110
Size: 530 26 18 86 6 153 291
Fragments arranged by size:
530 291 153 86 26 18 6
KasI G'GCGC_C
Cuts at: 0 815 1110
Size: 815 295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 1110
Size: 338 772
MscI TGG'CCA
Cuts at: 0 544 1082 1110
Size: 544 538 28
Fragments arranged by size:
544 538 28
MslI CAynn'nnrTG
Cuts at: 0 466 1110
Size: 466 644
MspA1I CmG'CkG
Cuts at: 0 162 174 498 562 879 951 1110
Size: 162 12 324 64 317 72 159
Fragments arranged by size:
324 317 162 159 72 64 12
NaeI GCC'GGC
Cuts at: 0 59 268 820 1110
Size: 59 209 552 290
Fragments arranged by size:
552 290 209 59
NarI GG'CG_CC
Cuts at: 0 816 1110
Size: 816 294
NcoI C'CATG_G
Cuts at: 0 1077 1110
Size: 1077 33
NgoMIV G'CCGG_C
Cuts at: 0 57 266 818 1110
Size: 57 209 552 292
Fragments arranged by size:
552 292 209 57
PasI CC'CwG_GG
Cuts at: 0 26 1110
Size: 26 1084
PmlI CAC'GTG
Cuts at: 0 600 1110
Size: 600 510
PpuMI rG'GwC_Cy
Cuts at: 0 903 1110
Size: 903 207
PspOMI G'GGCC_C
Cuts at: 0 21 1110
Size: 21 1089
PstI C_TGCA'G
Cuts at: 0 161 422 1110
Size: 161 261 688
Fragments arranged by size:
688 261 161
PvuII CAG'CTG
Cuts at: 0 162 174 951 1110
Size: 162 12 777 159
Fragments arranged by size:
777 162 159 12
SacI G_AGCT'C
Cuts at: 0 608 1110
Size: 608 502
SacII CC_GC'GG
Cuts at: 0 563 1110
Size: 563 547
SexAI A'CCwGG_T
Cuts at: 0 404 1110
Size: 404 706
SfcI C'TryA_G
Cuts at: 0 157 198 418 1110
Size: 157 41 220 692
Fragments arranged by size:
692 220 157 41
SfoI GGC'GCC
Cuts at: 0 817 1110
Size: 817 293
SgrAI Cr'CCGG_yG
Cuts at: 0 57 818 1110
Size: 57 761 292
Fragments arranged by size:
761 292 57
SmlI C'TyrA_G
Cuts at: 0 607 1110
Size: 607 503
StuI AGG'CCT
Cuts at: 0 94 1110
Size: 94 1016
StyI C'CwwG_G
Cuts at: 0 1077 1110
Size: 1077 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 369 1110
Size: 369 741
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 465 1110
Size: 465 645
Enzymes that do cut:
AcuI AlfI AlwNI ApaI ApaLI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaAI BsaHI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII BstXI
BtgI BtgZI BtsI DraIII DrdI EaeI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV PasI PmlI PpuMI
PspOMI PstI PvuII SacI SacII SexAI SfcI SfoI
SgrAI SmlI StuI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BfrBI BglII BlpI
BmtI BplI BsaI BsaBI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstYI BstZ17I Bsu36I ClaI CspCI DraI EagI
EarI EciI EcoRI EcoRV FalI FseI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SapI SbfI ScaI SfiI SgfI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI