(Linear) (Six-base) MAPSORT of: KIEE9514.seq  Check: 473  from: 1  to: 1185



KIEE9514.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:41  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     547    1185 
   Size:        547     638
 
AgeI A'CCGG_T

Cuts at:      0     136    1185 
   Size:        136    1049
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1130    1139    1185 
   Size:       1130       9      46

  Fragments arranged by size: 

                1130      46       9
 
ApoI r'AATT_y

Cuts at:      0     309     487    1185 
   Size:        309     178     698

  Fragments arranged by size: 

                 698     309     178
 
AvrII C'CTAG_G

Cuts at:      0     577    1185 
   Size:        577     608
 
BanI G'GyrC_C

Cuts at:      0     386    1185 
   Size:        386     799
 
BanII G_rGCy'C

Cuts at:      0     827    1185 
   Size:        827     358
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     546    1185 
   Size:        546     639
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     831     865    1185 
   Size:        831      34     320

  Fragments arranged by size: 

                 831     320      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      47     105     346    1185 
   Size:         47      58     241     839

  Fragments arranged by size: 

                 839     241      58      47
 
BclI T'GATC_A

Cuts at:      0     563    1185 
   Size:        563     622
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     570    1185 
   Size:        570     615
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     567    1185 
   Size:        567     618
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     145     430     634    1185 
   Size:        145     285     204     551

  Fragments arranged by size: 

                 551     285     204     145
 
BsaBI GATnn'nnATC

Cuts at:      0     568    1185 
   Size:        568     617
 
BsaWI w'CCGG_w

Cuts at:      0     136    1185 
   Size:        136    1049
 
BseYI C'CCAG_C

Cuts at:      0     647    1114    1185 
   Size:        647     467      71

  Fragments arranged by size: 

                 647     467      71
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     132     218    1040    1185 
   Size:        132      86     822     145

  Fragments arranged by size: 

                 822     145     132      86
 
BsiHKAI G_wGCw'C

Cuts at:      0     967    1185 
   Size:        967     218
 
BsmI GAATG_Cn'

Cuts at:      0     121    1185 
   Size:        121    1064
 
Bsp1286I G_dGCh'C

Cuts at:      0     827     967    1185 
   Size:        827     140     218

  Fragments arranged by size: 

                 827     218     140
 
BspHI T'CATG_A

Cuts at:      0    1167    1185 
   Size:       1167      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     547     870    1185 
   Size:        547     323     315

  Fragments arranged by size: 

                 547     323     315
 
BsrDI GCAATG_nn'

Cuts at:      0    1064    1185 
   Size:       1064     121
 
BsrFI r'CCGG_y

Cuts at:      0     136    1185 
   Size:        136    1049
 
BsrGI T'GTAC_A

Cuts at:      0     863    1185 
   Size:        863     322
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1139    1185 
   Size:       1139      46
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     261     855    1185 
   Size:        261     594     330

  Fragments arranged by size: 

                 594     330     261
 
BstYI r'GATC_y

Cuts at:      0     364    1120    1185 
   Size:        364     756      65

  Fragments arranged by size: 

                 756     364      65
 
BtgI C'CryG_G

Cuts at:      0     440    1185 
   Size:        440     745
 
BtsI GCAGTG_nn'

Cuts at:      0    1049    1185 
   Size:       1049     136
 
DraIII CAC_nnn'GTG

Cuts at:      0     760    1185 
   Size:        760     425
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     177    1185 
   Size:        177    1008
 
EarI CTCTTCn'nnn_

Cuts at:      0     924    1024    1185 
   Size:        924     100     161

  Fragments arranged by size: 

                 924     161     100
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     570    1185 
   Size:        570     615
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26    1112    1144    1185 
   Size:         26    1086      32      41

  Fragments arranged by size: 

                1086      41      32      26
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     276    1102    1185 
   Size:        276     826      83

  Fragments arranged by size: 

                 826     276      83
 
MspA1I CmG'CkG

Cuts at:      0     177    1185 
   Size:        177    1008
 
NcoI C'CATG_G

Cuts at:      0     440    1185 
   Size:        440     745
 
NspI r_CATG'y

Cuts at:      0     663    1185 
   Size:        663     522
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     663    1037    1185 
   Size:        663     374     148

  Fragments arranged by size: 

                 663     374     148
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     892     924    1185 
   Size:        892      32     261

  Fragments arranged by size: 

                 892     261      32
 
PstI C_TGCA'G

Cuts at:      0    1135    1147    1185 
   Size:       1135      12      38

  Fragments arranged by size: 

                1135      38      12
 
PvuII CAG'CTG

Cuts at:      0     177    1185 
   Size:        177    1008
 
SfcI C'TryA_G

Cuts at:      0     395     894    1131    1143    1185 
   Size:        395     499     237      12      42

  Fragments arranged by size: 

                 499     395     237      42      12
 
SmlI C'TyrA_G

Cuts at:      0     546    1185 
   Size:        546     639
 
StyI C'CwwG_G

Cuts at:      0     407     440     577    1185 
   Size:        407      33     137     608

  Fragments arranged by size: 

                 608     407     137      33
 
TatI w'GTAC_w

Cuts at:      0     863    1185 
   Size:        863     322
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     258     292    1185 
   Size:        258      34     893

  Fragments arranged by size: 

                 893     258      34
 

 Enzymes that do cut:

     AarI     AgeI    AlwNI     ApoI    AvrII     BanI    BanII     BbsI
     BcgI    BciVI     BclI     BpmI    BpuEI     BsaI    BsaBI    BsaWI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BsrFI    BsrGI   BstAPI    BstXI    BstYI     BtgI     BtsI   DraIII
     DrdI     EarI  Eco57MI    Hin4I     MmeI   MspA1I     NcoI     NspI
    PflMI     PsrI     PstI    PvuII     SfcI     SmlI     StyI     TatI
     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BbeI    BbvCI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BsaAI    BsaHI    BsaXI    BseRI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI