Restriction map for
pFN21AE9509
(Linear) (Six-base) MAPSORT of: KIEE9509.seq Check: 6467 from: 1 to: 966
KIEE9509.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
Acc65I G'GTAC_C
Cuts at: 0 386 966
Size: 386 580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 966
Size: 880 86
AfeI AGC'GCT
Cuts at: 0 358 966
Size: 358 608
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 166 198 460 492 966
Size: 166 32 262 32 474
Fragments arranged by size:
474 262 166 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 81 858 966
Size: 81 777 108
Fragments arranged by size:
777 108 81
ApaI G_GGCC'C
Cuts at: 0 444 711 966
Size: 444 267 255
Fragments arranged by size:
444 267 255
BamHI G'GATC_C
Cuts at: 0 613 722 966
Size: 613 109 244
Fragments arranged by size:
613 244 109
BanI G'GyrC_C
Cuts at: 0 386 966
Size: 386 580
BanII G_rGCy'C
Cuts at: 0 444 711 829 882 966
Size: 444 267 118 53 84
Fragments arranged by size:
444 267 118 84 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 663 966
Size: 663 303
BciVI GTATCCnnnnn_n'
Cuts at: 0 472 966
Size: 472 494
BlpI GC'TnA_GC
Cuts at: 0 337 966
Size: 337 629
Bme1580I G_kGCm'C
Cuts at: 0 444 711 966
Size: 444 267 255
Fragments arranged by size:
444 267 255
BmgBI CAC'GTC
Cuts at: 0 52 101 966
Size: 52 49 865
Fragments arranged by size:
865 52 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 10 966
Size: 10 956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 856 958 966
Size: 487 369 102 8
Fragments arranged by size:
487 369 102 8
Bpu10I CC'TnA_GC
Cuts at: 0 82 226 328 966
Size: 82 144 102 638
Fragments arranged by size:
638 144 102 82
BsaAI yAC'GTr
Cuts at: 0 645 966
Size: 645 321
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 166 196 460 490 966
Size: 166 30 264 30 476
Fragments arranged by size:
476 264 166 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 646 667 966
Size: 70 576 21 299
Fragments arranged by size:
576 299 70 21
BsiEI CG_ry'CG
Cuts at: 0 212 966
Size: 212 754
BsiHKAI G_wGCw'C
Cuts at: 0 146 966
Size: 146 820
BsmI GAATG_Cn'
Cuts at: 0 698 966
Size: 698 268
Bsp1286I G_dGCh'C
Cuts at: 0 146 444 711 829 882 966
Size: 146 298 267 118 53 84
Fragments arranged by size:
298 267 146 118 84 53
BsrBI CCG'CTC
Cuts at: 0 784 966
Size: 784 182
BstYI r'GATC_y
Cuts at: 0 613 722 966
Size: 613 109 244
Fragments arranged by size:
613 244 109
Bsu36I CC'TnA_GG
Cuts at: 0 896 926 966
Size: 896 30 40
Fragments arranged by size:
896 40 30
BtsI GCAGTG_nn'
Cuts at: 0 390 801 966
Size: 390 411 165
Fragments arranged by size:
411 390 165
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 715 750 966
Size: 715 35 216
Fragments arranged by size:
715 216 35
EaeI y'GGCC_r
Cuts at: 0 209 231 966
Size: 209 22 735
Fragments arranged by size:
735 209 22
EagI C'GGCC_G
Cuts at: 0 209 966
Size: 209 757
EarI CTCTTCn'nnn_
Cuts at: 0 226 966
Size: 226 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 259 966
Size: 259 707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 856 880 958 966
Size: 487 369 24 78 8
Fragments arranged by size:
487 369 78 24 8
EcoO109I rG'GnC_Cy
Cuts at: 0 441 708 966
Size: 441 267 258
Fragments arranged by size:
441 267 258
FspI TGC'GCA
Cuts at: 0 169 966
Size: 169 797
HaeII r_GCGC'y
Cuts at: 0 344 360 966
Size: 344 16 606
Fragments arranged by size:
606 344 16
KpnI G_GTAC'C
Cuts at: 0 390 966
Size: 390 576
MspA1I CmG'CkG
Cuts at: 0 630 966
Size: 630 336
NspI r_CATG'y
Cuts at: 0 168 336 966
Size: 168 168 630
Fragments arranged by size:
630 168 168
PflMI CCAn_nnn'nTGG
Cuts at: 0 374 966
Size: 374 592
PfoI T'CCnGG_A
Cuts at: 0 464 616 718 966
Size: 464 152 102 248
Fragments arranged by size:
464 248 152 102
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 460 492 966
Size: 166 32 262 32 474
Fragments arranged by size:
474 262 166 32 32
PspOMI G'GGCC_C
Cuts at: 0 440 707 966
Size: 440 267 259
Fragments arranged by size:
440 267 259
PstI C_TGCA'G
Cuts at: 0 887 966
Size: 887 79
SexAI A'CCwGG_T
Cuts at: 0 200 966
Size: 200 766
SfcI C'TryA_G
Cuts at: 0 883 966
Size: 883 83
SphI G_CATG'C
Cuts at: 0 168 336 966
Size: 168 168 630
Fragments arranged by size:
630 168 168
StuI AGG'CCT
Cuts at: 0 326 966
Size: 326 640
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 408 966
Size: 408 558
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 465 497 594 626 966
Size: 465 32 97 32 340
Fragments arranged by size:
465 340 97 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 18 726 966
Size: 18 708 240
Fragments arranged by size:
708 240 18
Enzymes that do cut:
Acc65I AcuI AfeI AloI AlwNI ApaI BamHI BanI
BanII BbsI BciVI BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BsaAI BsaXI BseRI BsiEI BsiHKAI BsmI Bsp1286I
BsrBI BstYI Bsu36I BtsI CspCI EaeI EagI EarI
EciI Eco57MI EcoO109I FspI HaeII KpnI MspA1I NspI
PflMI PfoI PpiI PspOMI PstI SexAI SfcI SphI
StuI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AleI AlfI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BglI
BglII BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI
BseYI BsgI BsiWI BsmBI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgI BtgZI ClaI DraI DraIII DrdI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HincII
HindIII HpaI KasI MfeI MluI MmeI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StyI
SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI