Restriction map for
pFN21AE9508
(Linear) (Six-base) MAPSORT of: KIEE9508.seq Check: 3293 from: 1 to: 966
KIEE9508.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
Acc65I G'GTAC_C
Cuts at: 0 386 966
Size: 386 580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 966
Size: 880 86
AfeI AGC'GCT
Cuts at: 0 358 966
Size: 358 608
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 166 198 460 492 966
Size: 166 32 262 32 474
Fragments arranged by size:
474 262 166 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 81 858 966
Size: 81 777 108
Fragments arranged by size:
777 108 81
ApaI G_GGCC'C
Cuts at: 0 711 966
Size: 711 255
ApoI r'AATT_y
Cuts at: 0 732 966
Size: 732 234
BamHI G'GATC_C
Cuts at: 0 3 602 613 966
Size: 3 599 11 353
Fragments arranged by size:
599 353 11 3
BanI G'GyrC_C
Cuts at: 0 386 966
Size: 386 580
BanII G_rGCy'C
Cuts at: 0 711 829 882 966
Size: 711 118 53 84
Fragments arranged by size:
711 118 84 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 12 663 966
Size: 12 651 303
Fragments arranged by size:
651 303 12
BbvCI CC'TCA_GC
Cuts at: 0 226 966
Size: 226 740
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 273 307 966
Size: 273 34 659
Fragments arranged by size:
659 273 34
BlpI GC'TnA_GC
Cuts at: 0 337 966
Size: 337 629
Bme1580I G_kGCm'C
Cuts at: 0 711 966
Size: 711 255
BmgBI CAC'GTC
Cuts at: 0 52 101 966
Size: 52 49 865
Fragments arranged by size:
865 52 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 10 532 966
Size: 10 522 434
Fragments arranged by size:
522 434 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 856 958 966
Size: 487 369 102 8
Fragments arranged by size:
487 369 102 8
Bpu10I CC'TnA_GC
Cuts at: 0 82 226 966
Size: 82 144 740
Fragments arranged by size:
740 144 82
BsaAI yAC'GTr
Cuts at: 0 645 966
Size: 645 321
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 166 196 460 465 490 495 966
Size: 166 30 264 5 25 5 471
Fragments arranged by size:
471 264 166 30 25 5 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 667 966
Size: 70 597 299
Fragments arranged by size:
597 299 70
BsiEI CG_ry'CG
Cuts at: 0 422 966
Size: 422 544
BsiHKAI G_wGCw'C
Cuts at: 0 146 966
Size: 146 820
BsmI GAATG_Cn'
Cuts at: 0 698 966
Size: 698 268
Bsp1286I G_dGCh'C
Cuts at: 0 146 711 829 882 966
Size: 146 565 118 53 84
Fragments arranged by size:
565 146 118 84 53
BstXI CCAn_nnnn'nTGG
Cuts at: 0 545 966
Size: 545 421
BstYI r'GATC_y
Cuts at: 0 3 602 613 966
Size: 3 599 11 353
Fragments arranged by size:
599 353 11 3
Bsu36I CC'TnA_GG
Cuts at: 0 896 926 966
Size: 896 30 40
Fragments arranged by size:
896 40 30
EaeI y'GGCC_r
Cuts at: 0 203 966
Size: 203 763
EarI CTCTTCn'nnn_
Cuts at: 0 226 966
Size: 226 740
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 856 880 958 966
Size: 487 369 24 78 8
Fragments arranged by size:
487 369 78 24 8
EcoO109I rG'GnC_Cy
Cuts at: 0 441 707 708 966
Size: 441 266 1 258
Fragments arranged by size:
441 266 258 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 937 966
Size: 937 29
FspI TGC'GCA
Cuts at: 0 169 966
Size: 169 797
HaeII r_GCGC'y
Cuts at: 0 344 360 966
Size: 344 16 606
Fragments arranged by size:
606 344 16
KpnI G_GTAC'C
Cuts at: 0 390 966
Size: 390 576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 99 966
Size: 31 68 867
Fragments arranged by size:
867 68 31
MspA1I CmG'CkG
Cuts at: 0 392 418 630 966
Size: 392 26 212 336
Fragments arranged by size:
392 336 212 26
NspI r_CATG'y
Cuts at: 0 168 774 966
Size: 168 606 192
Fragments arranged by size:
606 192 168
PfoI T'CCnGG_A
Cuts at: 0 616 966
Size: 616 350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 460 492 966
Size: 166 32 262 32 474
Fragments arranged by size:
474 262 166 32 32
PspOMI G'GGCC_C
Cuts at: 0 707 966
Size: 707 259
PstI C_TGCA'G
Cuts at: 0 887 966
Size: 887 79
SfcI C'TryA_G
Cuts at: 0 883 966
Size: 883 83
SphI G_CATG'C
Cuts at: 0 168 966
Size: 168 798
StuI AGG'CCT
Cuts at: 0 326 966
Size: 326 640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 465 497 594 626 966
Size: 465 32 97 32 340
Fragments arranged by size:
465 340 97 32 32
Enzymes that do cut:
Acc65I AcuI AfeI AloI AlwNI ApaI ApoI BamHI
BanI BanII BbsI BbvCI BcgI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaAI BsaXI BseRI BsiEI BsiHKAI
BsmI Bsp1286I BstXI BstYI Bsu36I EaeI EarI Eco57MI
EcoO109I FalI FspI HaeII KpnI MmeI MspA1I NspI
PfoI PpiI PspOMI PstI SfcI SphI StuI TstI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BciVI BclI BfrBI BglI BglII BmtI
BplI BpuEI BsaI BsaBI BsaHI BsaWI BseYI BsgI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspAI Hin4I
HincII HindIII HpaI KasI MfeI MluI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StyI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI