Restriction map for
pFN21AE9507
(Linear) (Six-base) MAPSORT of: KIEE9507.seq Check: 7793 from: 1 to: 975
KIEE9507.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 26 975
Size: 26 949
AflIII A'CryG_T
Cuts at: 0 620 975
Size: 620 355
AhdI GACnn_n'nnGTC
Cuts at: 0 73 975
Size: 73 902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 405 975
Size: 405 570
AlwNI CAG_nnn'CTG
Cuts at: 0 269 975
Size: 269 706
ApaI G_GGCC'C
Cuts at: 0 756 975
Size: 756 219
ApoI r'AATT_y
Cuts at: 0 3 975
Size: 3 972
AvaI C'yCGr_G
Cuts at: 0 667 975
Size: 667 308
BanII G_rGCy'C
Cuts at: 0 756 975
Size: 756 219
BbsI GAAGACnn'nnnn_
Cuts at: 0 80 107 519 916 975
Size: 80 27 412 397 59
Fragments arranged by size:
412 397 80 59 27
Bme1580I G_kGCm'C
Cuts at: 0 756 975
Size: 756 219
BmgBI CAC'GTC
Cuts at: 0 645 975
Size: 645 330
Bpu10I CC'TnA_GC
Cuts at: 0 474 975
Size: 474 501
BsaI GGTCTCn'nnnn_
Cuts at: 0 268 975
Size: 268 707
BsaHI Gr'CG_yC
Cuts at: 0 323 975
Size: 323 652
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 975
Size: 187 30 758
Fragments arranged by size:
758 187 30
BseYI C'CCAG_C
Cuts at: 0 729 749 975
Size: 729 20 226
Fragments arranged by size:
729 226 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 246 411 492 975
Size: 184 62 165 81 483
Fragments arranged by size:
483 184 165 81 62
BsmI GAATG_Cn'
Cuts at: 0 48 975
Size: 48 927
Bsp1286I G_dGCh'C
Cuts at: 0 756 975
Size: 756 219
BspHI T'CATG_A
Cuts at: 0 458 975
Size: 458 517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 26 206 975
Size: 26 180 769
Fragments arranged by size:
769 180 26
BsrDI GCAATG_nn'
Cuts at: 0 110 404 975
Size: 110 294 571
Fragments arranged by size:
571 294 110
BsrFI r'CCGG_y
Cuts at: 0 490 975
Size: 490 485
BsrGI T'GTAC_A
Cuts at: 0 611 975
Size: 611 364
BstAPI GCAn_nnn'nTGC
Cuts at: 0 515 975
Size: 515 460
BstEII G'GTnAC_C
Cuts at: 0 711 975
Size: 711 264
BstXI CCAn_nnnn'nTGG
Cuts at: 0 411 729 975
Size: 411 318 246
Fragments arranged by size:
411 318 246
BstYI r'GATC_y
Cuts at: 0 30 975
Size: 30 945
BtgI C'CryG_G
Cuts at: 0 374 936 975
Size: 374 562 39
Fragments arranged by size:
562 374 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 471 975
Size: 471 504
BtsI GCAGTG_nn'
Cuts at: 0 242 416 975
Size: 242 174 559
Fragments arranged by size:
559 242 174
DraIII CAC_nnn'GTG
Cuts at: 0 97 312 975
Size: 97 215 663
Fragments arranged by size:
663 215 97
EaeI y'GGCC_r
Cuts at: 0 401 975
Size: 401 574
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 256 975
Size: 256 719
EcoO109I rG'GnC_Cy
Cuts at: 0 67 753 975
Size: 67 686 222
Fragments arranged by size:
686 222 67
EcoRI G'AATT_C
Cuts at: 0 3 975
Size: 3 972
HaeII r_GCGC'y
Cuts at: 0 474 975
Size: 474 501
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 59 91 645 677 858 890 975
Size: 59 32 554 32 181 32 85
Fragments arranged by size:
554 181 85 59 32 32 32
MscI TGG'CCA
Cuts at: 0 403 975
Size: 403 572
MslI CAynn'nnrTG
Cuts at: 0 115 250 373 387 409 574 619 778
Size: 115 135 123 14 22 165 45 159
Cuts at: 778 975
Size: 197
Fragments arranged by size:
197 165 159 135 123 115 45 22
14
NaeI GCC'GGC
Cuts at: 0 492 975
Size: 492 483
NgoMIV G'CCGG_C
Cuts at: 0 490 975
Size: 490 485
NspI r_CATG'y
Cuts at: 0 249 261 624 702 975
Size: 249 12 363 78 273
Fragments arranged by size:
363 273 249 78 12
PciI A'CATG_T
Cuts at: 0 620 975
Size: 620 355
PflMI CCAn_nnn'nTGG
Cuts at: 0 269 975
Size: 269 706
PpuMI rG'GwC_Cy
Cuts at: 0 67 975
Size: 67 908
PshAI GACnn'nnGTC
Cuts at: 0 643 975
Size: 643 332
PspOMI G'GGCC_C
Cuts at: 0 752 975
Size: 752 223
PstI C_TGCA'G
Cuts at: 0 958 975
Size: 958 17
SfcI C'TryA_G
Cuts at: 0 954 975
Size: 954 21
SmaI CCC'GGG
Cuts at: 0 669 975
Size: 669 306
SphI G_CATG'C
Cuts at: 0 702 975
Size: 702 273
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 127 975
Size: 127 848
TatI w'GTAC_w
Cuts at: 0 212 536 611 975
Size: 212 324 75 364
Fragments arranged by size:
364 324 212 75
XmaI C'CCGG_G
Cuts at: 0 667 975
Size: 667 308
Enzymes that do cut:
AarI AflIII AhdI AlfI AlwNI ApaI ApoI AvaI
BanII BbsI Bme1580I BmgBI Bpu10I BsaI BsaHI BsaXI
BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EciI EcoO109I EcoRI HaeII Hin4I MscI
MslI NaeI NgoMIV NspI PciI PflMI PpuMI PshAI
PspOMI PstI SfcI SmaI SphI TaqII TatI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmrI BmtI BplI BpmI BpuEI
BsaAI BsaBI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmBI
BspEI BsrBI BssHII BssSI BstBI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EarI EcoICRI Eco57MI EcoNI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MspA1I NarI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI StyI SwaI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI