Restriction map for
pFN21AE9505
(Linear) (Six-base) MAPSORT of: KIEE9505.seq Check: 2193 from: 1 to: 1011
KIEE9505.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 444 758 1011
Size: 444 314 253
Fragments arranged by size:
444 314 253
AccI GT'mk_AC
Cuts at: 0 427 1011
Size: 427 584
ApaI G_GGCC'C
Cuts at: 0 635 807 930 1011
Size: 635 172 123 81
Fragments arranged by size:
635 172 123 81
AvaI C'yCGr_G
Cuts at: 0 409 1011
Size: 409 602
BanI G'GyrC_C
Cuts at: 0 76 147 205 738 1011
Size: 76 71 58 533 273
Fragments arranged by size:
533 273 76 71 58
BanII G_rGCy'C
Cuts at: 0 635 807 891 930 1011
Size: 635 172 84 39 81
Fragments arranged by size:
635 172 84 81 39
BbeI G_GCGC'C
Cuts at: 0 80 1011
Size: 80 931
BglI GCCn_nnn'nGGC
Cuts at: 0 74 85 166 175 466 811 1011
Size: 74 11 81 9 291 345 200
Fragments arranged by size:
345 291 200 81 74 11 9
Bme1580I G_kGCm'C
Cuts at: 0 278 503 635 666 706 741 807 930
Size: 278 225 132 31 40 35 66 123
Cuts at: 930 1011
Size: 81
Fragments arranged by size:
278 225 132 123 81 66 40 35
31
BmrI ACTGGGnnnn_n'
Cuts at: 0 933 1011
Size: 933 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 1011
Size: 564 447
BsaI GGTCTCn'nnnn_
Cuts at: 0 999 1011
Size: 999 12
BsaHI Gr'CG_yC
Cuts at: 0 77 1011
Size: 77 934
BsaWI w'CCGG_w
Cuts at: 0 608 1011
Size: 608 403
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 189 219 559 589 1011
Size: 189 30 340 30 422
Fragments arranged by size:
422 340 189 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 567 1011
Size: 567 444
BseYI C'CCAG_C
Cuts at: 0 178 540 674 699 734 753 1011
Size: 178 362 134 25 35 19 258
Fragments arranged by size:
362 258 178 134 35 25 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 472 508 1011
Size: 329 143 36 503
Fragments arranged by size:
503 329 143 36
BsiEI CG_ry'CG
Cuts at: 0 697 1011
Size: 697 314
BsiHKAI G_wGCw'C
Cuts at: 0 536 767 1011
Size: 536 231 244
Fragments arranged by size:
536 244 231
BsmI GAATG_Cn'
Cuts at: 0 597 1011
Size: 597 414
Bsp1286I G_dGCh'C
Cuts at: 0 278 503 536 635 666 706 741 767
Size: 278 225 33 99 31 40 35 26
Cuts at: 767 807 891 930 1011
Size: 40 84 39 81
Fragments arranged by size:
278 225 99 84 81 40 40 39
35 33 31 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 444 758 1011
Size: 444 314 253
Fragments arranged by size:
444 314 253
BsrBI CCG'CTC
Cuts at: 0 114 1011
Size: 114 897
BsrDI GCAATG_nn'
Cuts at: 0 162 278 1011
Size: 162 116 733
Fragments arranged by size:
733 162 116
BsrFI r'CCGG_y
Cuts at: 0 518 692 899 1011
Size: 518 174 207 112
Fragments arranged by size:
518 207 174 112
BstXI CCAn_nnnn'nTGG
Cuts at: 0 138 717 1011
Size: 138 579 294
Fragments arranged by size:
579 294 138
Bsu36I CC'TnA_GG
Cuts at: 0 931 1011
Size: 931 80
BtgI C'CryG_G
Cuts at: 0 161 230 285 644 1011
Size: 161 69 55 359 367
Fragments arranged by size:
367 359 161 69 55
BtsI GCAGTG_nn'
Cuts at: 0 278 317 559 904 1011
Size: 278 39 242 345 107
Fragments arranged by size:
345 278 242 107 39
EaeI y'GGCC_r
Cuts at: 0 86 227 298 641 647 694 797 1011
Size: 86 141 71 343 6 47 103 214
Fragments arranged by size:
343 214 141 103 86 71 47 6
EagI C'GGCC_G
Cuts at: 0 694 1011
Size: 694 317
EarI CTCTTCn'nnn_
Cuts at: 0 874 1011
Size: 874 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 250 495 886 1011
Size: 250 245 391 125
Fragments arranged by size:
391 250 245 125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 1011
Size: 564 447
EcoO109I rG'GnC_Cy
Cuts at: 0 631 632 803 804 927 1011
Size: 631 1 171 1 123 84
Fragments arranged by size:
631 171 123 84 1 1
HaeII r_GCGC'y
Cuts at: 0 80 328 1011
Size: 80 248 683
Fragments arranged by size:
683 248 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 979 1011
Size: 979 32
KasI G'GCGC_C
Cuts at: 0 76 1011
Size: 76 935
MscI TGG'CCA
Cuts at: 0 88 649 799 1011
Size: 88 561 150 212
Fragments arranged by size:
561 212 150 88
MslI CAynn'nnrTG
Cuts at: 0 715 1011
Size: 715 296
MspA1I CmG'CkG
Cuts at: 0 99 438 653 699 851 913 1011
Size: 99 339 215 46 152 62 98
Fragments arranged by size:
339 215 152 99 98 62 46
NaeI GCC'GGC
Cuts at: 0 520 694 1011
Size: 520 174 317
Fragments arranged by size:
520 317 174
NarI GG'CG_CC
Cuts at: 0 77 1011
Size: 77 934
NcoI C'CATG_G
Cuts at: 0 161 1011
Size: 161 850
NgoMIV G'CCGG_C
Cuts at: 0 518 692 1011
Size: 518 174 319
Fragments arranged by size:
518 319 174
PasI CC'CwG_GG
Cuts at: 0 942 1011
Size: 942 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 753 990 1011
Size: 753 237 21
Fragments arranged by size:
753 237 21
PspOMI G'GGCC_C
Cuts at: 0 631 803 926 1011
Size: 631 172 123 85
Fragments arranged by size:
631 172 123 85
PstI C_TGCA'G
Cuts at: 0 554 1011
Size: 554 457
PvuII CAG'CTG
Cuts at: 0 653 851 913 1011
Size: 653 198 62 98
Fragments arranged by size:
653 198 98 62
RsrII CG'GwC_CG
Cuts at: 0 51 1011
Size: 51 960
SapI GCTCTTCn'nnn_
Cuts at: 0 874 1011
Size: 874 137
SexAI A'CCwGG_T
Cuts at: 0 211 989 1011
Size: 211 778 22
Fragments arranged by size:
778 211 22
SfcI C'TryA_G
Cuts at: 0 550 1011
Size: 550 461
SfoI GGC'GCC
Cuts at: 0 78 1011
Size: 78 933
StyI C'CwwG_G
Cuts at: 0 161 1011
Size: 161 850
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 162 193 1011
Size: 162 31 818
Fragments arranged by size:
818 162 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 189 221 229 261 1011
Size: 189 32 8 32 750
Fragments arranged by size:
750 189 32 32 8
Tth111I GACn'n_nGTC
Cuts at: 0 991 1011
Size: 991 20
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 138 301 987 1011
Size: 138 163 686 24
Fragments arranged by size:
686 163 138 24
Enzymes that do cut:
AarI AccI ApaI AvaI BanI BanII BbeI BglI
Bme1580I BmrI BpmI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstXI Bsu36I BtgI BtsI EaeI EagI
EarI EciI Eco57MI EcoO109I HaeII Hin4I KasI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI
PspOMI PstI PvuII RsrII SapI SexAI SfcI SfoI
StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsiWI BsmBI BspEI BspHI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NdeI NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI SacI
SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI XbaI XhoI XmaI XmnI ZraI