Restriction map for
pFN21AE9502
(Linear) (Six-base) MAPSORT of: KIEE9502.seq Check: 8970 from: 1 to: 999
KIEE9502.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 323 999
Size: 323 676
AccI GT'mk_AC
Cuts at: 0 368 560 641 999
Size: 368 192 81 358
Fragments arranged by size:
368 358 192 81
AflIII A'CryG_T
Cuts at: 0 242 644 999
Size: 242 402 355
Fragments arranged by size:
402 355 242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 373 589 999
Size: 373 216 410
Fragments arranged by size:
410 373 216
ApaLI G'TGCA_C
Cuts at: 0 675 999
Size: 675 324
AvaI C'yCGr_G
Cuts at: 0 610 999
Size: 610 389
BanI G'GyrC_C
Cuts at: 0 14 64 216 999
Size: 14 50 152 783
Fragments arranged by size:
783 152 50 14
BanII G_rGCy'C
Cuts at: 0 26 43 278 999
Size: 26 17 235 721
Fragments arranged by size:
721 235 26 17
BbeI G_GCGC'C
Cuts at: 0 220 999
Size: 220 779
BbsI GAAGACnn'nnnn_
Cuts at: 0 235 999
Size: 235 764
BbvCI CC'TCA_GC
Cuts at: 0 910 999
Size: 910 89
BlpI GC'TnA_GC
Cuts at: 0 595 999
Size: 595 404
Bme1580I G_kGCm'C
Cuts at: 0 17 679 999
Size: 17 662 320
Fragments arranged by size:
662 320 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 758 999
Size: 372 386 241
Fragments arranged by size:
386 372 241
Bpu10I CC'TnA_GC
Cuts at: 0 910 999
Size: 910 89
BsaHI Gr'CG_yC
Cuts at: 0 217 518 557 820 999
Size: 217 301 39 263 179
Fragments arranged by size:
301 263 217 179 39
BsaWI w'CCGG_w
Cuts at: 0 968 999
Size: 968 31
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 370 400 999
Size: 370 30 599
Fragments arranged by size:
599 370 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 799 999
Size: 799 200
BseYI C'CCAG_C
Cuts at: 0 10 513 999
Size: 10 503 486
Fragments arranged by size:
503 486 10
BsiEI CG_ry'CG
Cuts at: 0 194 999
Size: 194 805
BsiHKAI G_wGCw'C
Cuts at: 0 140 314 679 692 999
Size: 140 174 365 13 307
Fragments arranged by size:
365 307 174 140 13
BsmBI CGTCTCn'nnnn_
Cuts at: 0 78 999
Size: 78 921
Bsp1286I G_dGCh'C
Cuts at: 0 17 26 43 140 278 314 679 692
Size: 17 9 17 97 138 36 365 13
Cuts at: 692 999
Size: 307
Fragments arranged by size:
365 307 138 97 36 17 17 13
9
BspEI T'CCGG_A
Cuts at: 0 968 999
Size: 968 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 323 567 606 869 999
Size: 323 244 39 263 130
Fragments arranged by size:
323 263 244 130 39
BsrBI CCG'CTC
Cuts at: 0 734 999
Size: 734 265
BssSI C'ACGA_G
Cuts at: 0 924 999
Size: 924 75
BstAPI GCAn_nnn'nTGC
Cuts at: 0 318 999
Size: 318 681
BstEII G'GTnAC_C
Cuts at: 0 234 771 999
Size: 234 537 228
Fragments arranged by size:
537 234 228
BstYI r'GATC_y
Cuts at: 0 161 999
Size: 161 838
Bsu36I CC'TnA_GG
Cuts at: 0 211 999
Size: 211 788
BtgI C'CryG_G
Cuts at: 0 604 854 999
Size: 604 250 145
Fragments arranged by size:
604 250 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 288 999
Size: 288 711
BtsI GCAGTG_nn'
Cuts at: 0 118 999
Size: 118 881
EaeI y'GGCC_r
Cuts at: 0 93 191 260 362 440 797 999
Size: 93 98 69 102 78 357 202
Fragments arranged by size:
357 202 102 98 93 78 69
EagI C'GGCC_G
Cuts at: 0 191 999
Size: 191 808
EarI CTCTTCn'nnn_
Cuts at: 0 283 999
Size: 283 716
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 780 999
Size: 780 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 758 999
Size: 372 386 241
Fragments arranged by size:
386 372 241
EcoO109I rG'GnC_Cy
Cuts at: 0 452 579 999
Size: 452 127 420
Fragments arranged by size:
452 420 127
FspI TGC'GCA
Cuts at: 0 709 999
Size: 709 290
HaeII r_GCGC'y
Cuts at: 0 220 999
Size: 220 779
HincII GTy'rAC
Cuts at: 0 300 369 999
Size: 300 69 630
Fragments arranged by size:
630 300 69
KasI G'GCGC_C
Cuts at: 0 216 999
Size: 216 783
MscI TGG'CCA
Cuts at: 0 95 442 999
Size: 95 347 557
Fragments arranged by size:
557 347 95
MslI CAynn'nnrTG
Cuts at: 0 685 886 999
Size: 685 201 113
Fragments arranged by size:
685 201 113
MspA1I CmG'CkG
Cuts at: 0 10 999
Size: 10 989
NarI GG'CG_CC
Cuts at: 0 217 999
Size: 217 782
NspI r_CATG'y
Cuts at: 0 465 999
Size: 465 534
PflMI CCAn_nnn'nTGG
Cuts at: 0 589 999
Size: 589 410
PfoI T'CCnGG_A
Cuts at: 0 157 999
Size: 157 842
PpuMI rG'GwC_Cy
Cuts at: 0 452 579 999
Size: 452 127 420
Fragments arranged by size:
452 420 127
PstI C_TGCA'G
Cuts at: 0 323 578 999
Size: 323 255 421
Fragments arranged by size:
421 323 255
SalI G'TCGA_C
Cuts at: 0 367 999
Size: 367 632
SapI GCTCTTCn'nnn_
Cuts at: 0 283 999
Size: 283 716
ScaI AGT'ACT
Cuts at: 0 325 999
Size: 325 674
SexAI A'CCwGG_T
Cuts at: 0 428 999
Size: 428 571
SfcI C'TryA_G
Cuts at: 0 319 561 574 999
Size: 319 242 13 425
Fragments arranged by size:
425 319 242 13
SfoI GGC'GCC
Cuts at: 0 218 999
Size: 218 781
StyI C'CwwG_G
Cuts at: 0 743 999
Size: 743 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 32 52 437 999
Size: 32 20 385 562
Fragments arranged by size:
562 385 32 20
TatI w'GTAC_w
Cuts at: 0 152 323 999
Size: 152 171 676
Fragments arranged by size:
676 171 152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 110 142 999
Size: 110 32 857
Fragments arranged by size:
857 110 32
XbaI T'CTAG_A
Cuts at: 0 954 999
Size: 954 45
XmnI GAAnn'nnTTC
Cuts at: 0 937 976 999
Size: 937 39 23
Fragments arranged by size:
937 39 23
Enzymes that do cut:
AarI AccI AflIII AlfI ApaLI AvaI BanI BanII
BbeI BbsI BbvCI BlpI Bme1580I BpmI Bpu10I BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BssSI BstAPI BstEII BstYI Bsu36I
BtgI BtgZI BtsI EaeI EagI EarI EciI Eco57MI
EcoO109I FspI HaeII HincII KasI MscI MslI MspA1I
NarI NspI PflMI PfoI PpuMI PstI SalI SapI
ScaI SexAI SfcI SfoI StyI TaqII TatI TstI
XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsgI BsiWI BsmI BspHI BsrDI BsrFI BsrGI
BssHII BstBI BstXI BstZ17I ClaI CspCI DraI DraIII
DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI
Hin4I HindIII HpaI KpnI MfeI MluI MmeI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SanDI
SbfI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XcmI XhoI
XmaI ZraI