Restriction map for
pFN21AE9461
(Linear) (Six-base) MAPSORT of: KIEE9461.seq Check: 8099 from: 1 to: 1443
KIEE9461.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 988 1443
Size: 988 455
AccI GT'mk_AC
Cuts at: 0 1100 1443
Size: 1100 343
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1443
Size: 502 941
AleI CACnn'nnGTG
Cuts at: 0 1081 1237 1443
Size: 1081 156 206
Fragments arranged by size:
1081 206 156
AlwNI CAG_nnn'CTG
Cuts at: 0 303 662 1179 1443
Size: 303 359 517 264
Fragments arranged by size:
517 359 303 264
AvaI C'yCGr_G
Cuts at: 0 883 1443
Size: 883 560
BanI G'GyrC_C
Cuts at: 0 73 139 280 324 731 841 1225 1443
Size: 73 66 141 44 407 110 384 218
Fragments arranged by size:
407 384 218 141 110 73 66 44
BanII G_rGCy'C
Cuts at: 0 379 775 800 1443
Size: 379 396 25 643
Fragments arranged by size:
643 396 379 25
BbeI G_GCGC'C
Cuts at: 0 1229 1443
Size: 1229 214
BbvCI CC'TCA_GC
Cuts at: 0 870 1443
Size: 870 573
BciVI GTATCCnnnnn_n'
Cuts at: 0 371 1443
Size: 371 1072
BfrBI ATG'CAT
Cuts at: 0 857 1443
Size: 857 586
BglI GCCn_nnn'nGGC
Cuts at: 0 514 754 1443
Size: 514 240 689
Fragments arranged by size:
689 514 240
BlpI GC'TnA_GC
Cuts at: 0 55 1443
Size: 55 1388
Bme1580I G_kGCm'C
Cuts at: 0 78 142 283 327 620 669 844 1435
Size: 78 64 141 44 293 49 175 591
Cuts at: 1435 1443
Size: 8
Fragments arranged by size:
591 293 175 141 78 64 49 44
8
BmrI ACTGGGnnnn_n'
Cuts at: 0 601 1231 1443
Size: 601 630 212
Fragments arranged by size:
630 601 212
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 651 1395 1443
Size: 651 744 48
Fragments arranged by size:
744 651 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 998 1443
Size: 998 445
Bpu10I CC'TnA_GC
Cuts at: 0 794 870 1443
Size: 794 76 573
Fragments arranged by size:
794 573 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1348 1443
Size: 1348 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 69 100 636 1443
Size: 69 31 536 807
Fragments arranged by size:
807 536 69 31
BsaAI yAC'GTr
Cuts at: 0 690 1443
Size: 690 753
BsaHI Gr'CG_yC
Cuts at: 0 1226 1443
Size: 1226 217
BsaWI w'CCGG_w
Cuts at: 0 363 1443
Size: 363 1080
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1192 1222 1403 1433 1443
Size: 1192 30 181 30 10
Fragments arranged by size:
1192 181 30 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 199 624 1324 1443
Size: 166 33 425 700 119
Fragments arranged by size:
700 425 166 119 33
BseYI C'CCAG_C
Cuts at: 0 1266 1443
Size: 1266 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 187 1053 1095 1443
Size: 67 120 866 42 348
Fragments arranged by size:
866 348 120 67 42
BsiHKAI G_wGCw'C
Cuts at: 0 379 800 1443
Size: 379 421 643
Fragments arranged by size:
643 421 379
Bsp1286I G_dGCh'C
Cuts at: 0 78 142 283 327 379 620 669 775
Size: 78 64 141 44 52 241 49 106
Cuts at: 775 800 844 1435 1443
Size: 25 44 591 8
Fragments arranged by size:
591 241 141 106 78 64 52 49
44 44 25 8
BspHI T'CATG_A
Cuts at: 0 860 1443
Size: 860 583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 988 1443
Size: 988 455
BsrBI CCG'CTC
Cuts at: 0 729 1443
Size: 729 714
BssSI C'ACGA_G
Cuts at: 0 1048 1443
Size: 1048 395
BstAPI GCAn_nnn'nTGC
Cuts at: 0 177 1439 1443
Size: 177 1262 4
Fragments arranged by size:
1262 177 4
BstEII G'GTnAC_C
Cuts at: 0 366 1443
Size: 366 1077
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 783 1443
Size: 243 540 660
Fragments arranged by size:
660 540 243
BstYI r'GATC_y
Cuts at: 0 344 1443
Size: 344 1099
Bsu36I CC'TnA_GG
Cuts at: 0 182 1443
Size: 182 1261
BtgI C'CryG_G
Cuts at: 0 836 1079 1094 1443
Size: 836 243 15 349
Fragments arranged by size:
836 349 243 15
BtsI GCAGTG_nn'
Cuts at: 0 1060 1443
Size: 1060 383
DrdI GACnn_nn'nnGTC
Cuts at: 0 1374 1443
Size: 1374 69
EaeI y'GGCC_r
Cuts at: 0 248 692 746 1443
Size: 248 444 54 697
Fragments arranged by size:
697 444 248 54
EarI CTCTTCn'nnn_
Cuts at: 0 478 973 1386 1443
Size: 478 495 413 57
Fragments arranged by size:
495 478 413 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 208 1443
Size: 208 1235
EcoICRI GAG'CTC
Cuts at: 0 377 798 1443
Size: 377 421 645
Fragments arranged by size:
645 421 377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 998 1443
Size: 502 496 445
Fragments arranged by size:
502 496 445
EcoNI CCTnn'n_nnAGG
Cuts at: 0 709 1443
Size: 709 734
EcoO109I rG'GnC_Cy
Cuts at: 0 1009 1443
Size: 1009 434
HaeII r_GCGC'y
Cuts at: 0 412 491 1229 1443
Size: 412 79 738 214
Fragments arranged by size:
738 412 214 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 297 329 1443
Size: 297 32 1114
Fragments arranged by size:
1114 297 32
KasI G'GCGC_C
Cuts at: 0 1225 1443
Size: 1225 218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1331 1443
Size: 1331 112
MscI TGG'CCA
Cuts at: 0 250 694 748 1443
Size: 250 444 54 695
Fragments arranged by size:
695 444 250 54
MslI CAynn'nnrTG
Cuts at: 0 766 1081 1141 1237 1443
Size: 766 315 60 96 206
Fragments arranged by size:
766 315 206 96 60
MspA1I CmG'CkG
Cuts at: 0 813 987 1266 1443
Size: 813 174 279 177
Fragments arranged by size:
813 279 177 174
NarI GG'CG_CC
Cuts at: 0 1226 1443
Size: 1226 217
NcoI C'CATG_G
Cuts at: 0 836 1443
Size: 836 607
NsiI A_TGCA'T
Cuts at: 0 859 1443
Size: 859 584
NspI r_CATG'y
Cuts at: 0 930 1443
Size: 930 513
PasI CC'CwG_GG
Cuts at: 0 669 1403 1428 1443
Size: 669 734 25 15
Fragments arranged by size:
734 669 25 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 292 1179 1443
Size: 292 887 264
Fragments arranged by size:
887 292 264
PfoI T'CCnGG_A
Cuts at: 0 1280 1443
Size: 1280 163
PmlI CAC'GTG
Cuts at: 0 690 1443
Size: 690 753
PshAI GACnn'nnGTC
Cuts at: 0 1096 1443
Size: 1096 347
PstI C_TGCA'G
Cuts at: 0 230 1261 1443
Size: 230 1031 182
Fragments arranged by size:
1031 230 182
PvuII CAG'CTG
Cuts at: 0 813 987 1443
Size: 813 174 456
Fragments arranged by size:
813 456 174
SacI G_AGCT'C
Cuts at: 0 379 800 1443
Size: 379 421 643
Fragments arranged by size:
643 421 379
SapI GCTCTTCn'nnn_
Cuts at: 0 478 1443
Size: 478 965
SfcI C'TryA_G
Cuts at: 0 226 340 381 1257 1443
Size: 226 114 41 876 186
Fragments arranged by size:
876 226 186 114 41
SfoI GGC'GCC
Cuts at: 0 1227 1443
Size: 1227 216
SmlI C'TyrA_G
Cuts at: 0 1363 1443
Size: 1363 80
StuI AGG'CCT
Cuts at: 0 1276 1333 1443
Size: 1276 57 110
Fragments arranged by size:
1276 110 57
StyI C'CwwG_G
Cuts at: 0 158 242 710 836 1443
Size: 158 84 468 126 607
Fragments arranged by size:
607 468 158 126 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 385 438 583 756 1384 1443
Size: 385 53 145 173 628 59
Fragments arranged by size:
628 385 173 145 59 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1403 1435 1443
Size: 1403 32 8
Fragments arranged by size:
1403 32 8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 243 402 843 1443
Size: 243 159 441 600
Fragments arranged by size:
600 441 243 159
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI AvaI BanI BanII
BbeI BbvCI BciVI BfrBI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MmeI MscI MslI MspA1I
NarI NcoI NsiI NspI PasI PflMI PfoI PmlI
PshAI PstI PvuII SacI SapI SfcI SfoI SmlI
StuI StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BclI BglII BmgBI
BmtI BsaBI BsiEI BsiWI BsmI BsmBI BspEI BsrDI
BsrFI BsrGI BssHII BstBI BstZ17I BtgZI ClaI CspCI
DraI DraIII EagI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI PacI PciI PmeI
PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI ZraI