Restriction map for
pFN21AE9459
(Linear) (Six-base) MAPSORT of: KIEE9459.seq Check: 8570 from: 1 to: 1587
KIEE9459.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1126 1587
Size: 1126 461
AccI GT'mk_AC
Cuts at: 0 446 779 1587
Size: 446 333 808
Fragments arranged by size:
808 446 333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 728 1059 1167 1379 1587
Size: 183 545 331 108 212 208
Fragments arranged by size:
545 331 212 208 183 108
AfeI AGC'GCT
Cuts at: 0 948 1587
Size: 948 639
AflIII A'CryG_T
Cuts at: 0 1232 1587
Size: 1232 355
AgeI A'CCGG_T
Cuts at: 0 1025 1587
Size: 1025 562
AhdI GACnn_n'nnGTC
Cuts at: 0 1233 1587
Size: 1233 354
AlwNI CAG_nnn'CTG
Cuts at: 0 1149 1536 1587
Size: 1149 387 51
Fragments arranged by size:
1149 387 51
ApaI G_GGCC'C
Cuts at: 0 853 1587
Size: 853 734
ApaLI G'TGCA_C
Cuts at: 0 664 1587
Size: 664 923
AvaI C'yCGr_G
Cuts at: 0 352 906 1482 1587
Size: 352 554 576 105
Fragments arranged by size:
576 554 352 105
BanI G'GyrC_C
Cuts at: 0 545 829 1587
Size: 545 284 758
Fragments arranged by size:
758 545 284
BanII G_rGCy'C
Cuts at: 0 262 853 1288 1587
Size: 262 591 435 299
Fragments arranged by size:
591 435 299 262
BbsI GAAGACnn'nnnn_
Cuts at: 0 189 1587
Size: 189 1398
BbvCI CC'TCA_GC
Cuts at: 0 19 181 1587
Size: 19 162 1406
Fragments arranged by size:
1406 162 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 174 208 1587
Size: 174 34 1379
Fragments arranged by size:
1379 174 34
BclI T'GATC_A
Cuts at: 0 326 1587
Size: 326 1261
BglI GCCn_nnn'nGGC
Cuts at: 0 877 1152 1411 1508 1587
Size: 877 275 259 97 79
Fragments arranged by size:
877 275 259 97 79
BglII A'GATC_T
Cuts at: 0 578 1587
Size: 578 1009
Bme1580I G_kGCm'C
Cuts at: 0 591 668 853 1157 1587
Size: 591 77 185 304 430
Fragments arranged by size:
591 430 304 185 77
BmgBI CAC'GTC
Cuts at: 0 1235 1587
Size: 1235 352
BmrI ACTGGGnnnn_n'
Cuts at: 0 81 1015 1587
Size: 81 934 572
Fragments arranged by size:
934 572 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 934 1587
Size: 708 226 653
Fragments arranged by size:
708 653 226
Bpu10I CC'TnA_GC
Cuts at: 0 19 181 1587
Size: 19 162 1406
Fragments arranged by size:
1406 162 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1243 1587
Size: 1243 344
BsaHI Gr'CG_yC
Cuts at: 0 74 1587
Size: 74 1513
BsaWI w'CCGG_w
Cuts at: 0 1025 1587
Size: 1025 562
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3 67 70 163 304 706 729 1456
Size: 3 64 3 93 141 402 23 727
Cuts at: 1456 1587
Size: 131
Fragments arranged by size:
727 402 141 131 93 64 23 3
3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1245 1587
Size: 1245 342
BsiEI CG_ry'CG
Cuts at: 0 372 1587
Size: 372 1215
BsiHKAI G_wGCw'C
Cuts at: 0 262 668 1288 1587
Size: 262 406 620 299
Fragments arranged by size:
620 406 299 262
BsmI GAATG_Cn'
Cuts at: 0 765 1587
Size: 765 822
BsmBI CGTCTCn'nnnn_
Cuts at: 0 906 1587
Size: 906 681
Bsp1286I G_dGCh'C
Cuts at: 0 262 591 668 853 1157 1288 1587
Size: 262 329 77 185 304 131 299
Fragments arranged by size:
329 304 299 262 185 131 77
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 329 1126 1587
Size: 329 797 461
Fragments arranged by size:
797 461 329
BsrDI GCAATG_nn'
Cuts at: 0 359 1587
Size: 359 1228
BsrFI r'CCGG_y
Cuts at: 0 1025 1587
Size: 1025 562
BsrGI T'GTAC_A
Cuts at: 0 155 1587
Size: 155 1432
BstEII G'GTnAC_C
Cuts at: 0 147 840 1587
Size: 147 693 747
Fragments arranged by size:
747 693 147
BstXI CCAn_nnnn'nTGG
Cuts at: 0 819 1587
Size: 819 768
BstYI r'GATC_y
Cuts at: 0 278 578 1587
Size: 278 300 1009
Fragments arranged by size:
1009 300 278
BtgI C'CryG_G
Cuts at: 0 395 503 1587
Size: 395 108 1084
Fragments arranged by size:
1084 395 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 78 852 1587
Size: 78 774 735
Fragments arranged by size:
774 735 78
BtsI GCAGTG_nn'
Cuts at: 0 1121 1257 1587
Size: 1121 136 330
Fragments arranged by size:
1121 330 136
EaeI y'GGCC_r
Cuts at: 0 641 785 1397 1587
Size: 641 144 612 190
Fragments arranged by size:
641 612 190 144
EarI CTCTTCn'nnn_
Cuts at: 0 598 747 1078 1587
Size: 598 149 331 509
Fragments arranged by size:
598 509 331 149
EcoICRI GAG'CTC
Cuts at: 0 260 1286 1587
Size: 260 1026 301
Fragments arranged by size:
1026 301 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 708 728 934 1059 1167 1379 1587
Size: 183 525 20 206 125 108 212 208
Fragments arranged by size:
525 212 208 206 183 125 108 20
EcoO109I rG'GnC_Cy
Cuts at: 0 849 850 879 1587
Size: 849 1 29 708
Fragments arranged by size:
849 708 29 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 213 1587
Size: 213 1374
HaeII r_GCGC'y
Cuts at: 0 950 1587
Size: 950 637
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1219 1251 1587
Size: 1219 32 336
Fragments arranged by size:
1219 336 32
HindIII A'AGCT_T
Cuts at: 0 1392 1587
Size: 1392 195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1109 1587
Size: 239 870 478
Fragments arranged by size:
870 478 239
MscI TGG'CCA
Cuts at: 0 643 1399 1587
Size: 643 756 188
Fragments arranged by size:
756 643 188
MslI CAynn'nnrTG
Cuts at: 0 613 817 1587
Size: 613 204 770
Fragments arranged by size:
770 613 204
MspA1I CmG'CkG
Cuts at: 0 23 1187 1587
Size: 23 1164 400
Fragments arranged by size:
1164 400 23
NcoI C'CATG_G
Cuts at: 0 395 1587
Size: 395 1192
NspI r_CATG'y
Cuts at: 0 345 1014 1587
Size: 345 669 573
Fragments arranged by size:
669 573 345
PasI CC'CwG_GG
Cuts at: 0 1149 1587
Size: 1149 438
PspOMI G'GGCC_C
Cuts at: 0 849 1587
Size: 849 738
PstI C_TGCA'G
Cuts at: 0 28 1541 1587
Size: 28 1513 46
Fragments arranged by size:
1513 46 28
PvuII CAG'CTG
Cuts at: 0 23 1187 1587
Size: 23 1164 400
Fragments arranged by size:
1164 400 23
SacI G_AGCT'C
Cuts at: 0 262 1288 1587
Size: 262 1026 299
Fragments arranged by size:
1026 299 262
SapI GCTCTTCn'nnn_
Cuts at: 0 1078 1587
Size: 1078 509
SexAI A'CCwGG_T
Cuts at: 0 134 1587
Size: 134 1453
SfcI C'TryA_G
Cuts at: 0 24 1537 1587
Size: 24 1513 50
Fragments arranged by size:
1513 50 24
SmaI CCC'GGG
Cuts at: 0 1484 1587
Size: 1484 103
SmlI C'TyrA_G
Cuts at: 0 1222 1587
Size: 1222 365
SphI G_CATG'C
Cuts at: 0 345 1587
Size: 345 1242
StyI C'CwwG_G
Cuts at: 0 395 1373 1587
Size: 395 978 214
Fragments arranged by size:
978 395 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 100 1287 1587
Size: 100 1187 300
Fragments arranged by size:
1187 300 100
TatI w'GTAC_w
Cuts at: 0 155 1362 1587
Size: 155 1207 225
Fragments arranged by size:
1207 225 155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 270 302 1463 1495 1587
Size: 270 32 1161 32 92
Fragments arranged by size:
1161 270 92 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 210 1587
Size: 210 1377
XmaI C'CCGG_G
Cuts at: 0 1482 1587
Size: 1482 105
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AhdI AlwNI
ApaI ApaLI AvaI BanI BanII BbsI BbvCI BcgI
BclI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaHI BsaWI BseRI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BstEII BstXI
BstYI BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI
EcoO109I FalI HaeII Hin4I HindIII MmeI MscI MslI
MspA1I NcoI NspI PasI PspOMI PstI PvuII SacI
SapI SexAI SfcI SmaI SmlI SphI StyI TaqII
TatI TstI Tth111I XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AleI AlfI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI
BfrBI BlpI BmtI BplI BsaI BsaAI BsaBI BsaXI
BseYI BsiWI BspEI BspHI BsrBI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI StuI SwaI XbaI XcmI XhoI XmnI ZraI