Restriction map for
pFN21AE9456
(Linear) (Six-base) MAPSORT of: KIEE9456.seq Check: 6341 from: 1 to: 2085
KIEE9456.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1222 2085
Size: 1222 863
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 2029 2085
Size: 137 1892 56
Fragments arranged by size:
1892 137 56
AflIII A'CryG_T
Cuts at: 0 229 265 1319 1412 2085
Size: 229 36 1054 93 673
Fragments arranged by size:
1054 673 229 93 36
AleI CACnn'nnGTG
Cuts at: 0 1144 2085
Size: 1144 941
ApoI r'AATT_y
Cuts at: 0 654 2085
Size: 654 1431
AseI AT'TA_AT
Cuts at: 0 2068 2085
Size: 2068 17
AvaI C'yCGr_G
Cuts at: 0 536 1283 2085
Size: 536 747 802
Fragments arranged by size:
802 747 536
BamHI G'GATC_C
Cuts at: 0 140 2085
Size: 140 1945
BanI G'GyrC_C
Cuts at: 0 1571 2085
Size: 1571 514
BanII G_rGCy'C
Cuts at: 0 372 1041 1226 2085
Size: 372 669 185 859
Fragments arranged by size:
859 669 372 185
BbsI GAAGACnn'nnnn_
Cuts at: 0 925 1994 2085
Size: 925 1069 91
Fragments arranged by size:
1069 925 91
BclI T'GATC_A
Cuts at: 0 1259 1580 2085
Size: 1259 321 505
Fragments arranged by size:
1259 505 321
BfrBI ATG'CAT
Cuts at: 0 859 2038 2085
Size: 859 1179 47
Fragments arranged by size:
1179 859 47
BglI GCCn_nnn'nGGC
Cuts at: 0 1693 2085
Size: 1693 392
BlpI GC'TnA_GC
Cuts at: 0 1225 2085
Size: 1225 860
Bme1580I G_kGCm'C
Cuts at: 0 1576 2085
Size: 1576 509
BmrI ACTGGGnnnn_n'
Cuts at: 0 612 1035 1665 2085
Size: 612 423 630 420
Fragments arranged by size:
630 612 423 420
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 410 2085
Size: 410 1675
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 356 2085
Size: 116 240 1729
Fragments arranged by size:
1729 240 116
Bpu10I CC'TnA_GC
Cuts at: 0 200 838 1240 2085
Size: 200 638 402 845
Fragments arranged by size:
845 638 402 200
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 2085
Size: 380 1705
BsaI GGTCTCn'nnnn_
Cuts at: 0 370 1626 2085
Size: 370 1256 459
Fragments arranged by size:
1256 459 370
BsaAI yAC'GTr
Cuts at: 0 1320 2085
Size: 1320 765
BseYI C'CCAG_C
Cuts at: 0 1839 2085
Size: 1839 246
BsiHKAI G_wGCw'C
Cuts at: 0 372 2085
Size: 372 1713
BsmI GAATG_Cn'
Cuts at: 0 2040 2085
Size: 2040 45
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1236 2085
Size: 1236 849
Bsp1286I G_dGCh'C
Cuts at: 0 372 1041 1226 1576 2085
Size: 372 669 185 350 509
Fragments arranged by size:
669 509 372 350 185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1222 2085
Size: 1222 863
BsrDI GCAATG_nn'
Cuts at: 0 1035 1483 1583 2085
Size: 1035 448 100 502
Fragments arranged by size:
1035 502 448 100
BsrGI T'GTAC_A
Cuts at: 0 268 2085
Size: 268 1817
BstBI TT'CG_AA
Cuts at: 0 1007 2085
Size: 1007 1078
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1048 2085
Size: 1048 1037
BstYI r'GATC_y
Cuts at: 0 73 140 2059 2085
Size: 73 67 1919 26
Fragments arranged by size:
1919 73 67 26
Bsu36I CC'TnA_GG
Cuts at: 0 1637 2085
Size: 1637 448
BtsI GCAGTG_nn'
Cuts at: 0 1346 1430 1834 2085
Size: 1346 84 404 251
Fragments arranged by size:
1346 404 251 84
ClaI AT'CG_AT
Cuts at: 0 1183 2085
Size: 1183 902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 226 261 2085
Size: 226 35 1824
Fragments arranged by size:
1824 226 35
DraIII CAC_nnn'GTG
Cuts at: 0 1277 2085
Size: 1277 808
EaeI y'GGCC_r
Cuts at: 0 566 850 2085
Size: 566 284 1235
Fragments arranged by size:
1235 566 284
EarI CTCTTCn'nnn_
Cuts at: 0 544 718 1480 2085
Size: 544 174 762 605
Fragments arranged by size:
762 605 544 174
EcoICRI GAG'CTC
Cuts at: 0 370 2085
Size: 370 1715
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 137 356 2029 2085
Size: 116 21 219 1673 56
Fragments arranged by size:
1673 219 116 56 21
EcoO109I rG'GnC_Cy
Cuts at: 0 160 1757 2085
Size: 160 1597 328
Fragments arranged by size:
1597 328 160
EcoRV GAT'ATC
Cuts at: 0 804 1107 1304 2085
Size: 804 303 197 781
Fragments arranged by size:
804 781 303 197
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2000 2085
Size: 2000 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2023 2055 2085
Size: 2023 32 30
Fragments arranged by size:
2023 32 30
HincII GTy'rAC
Cuts at: 0 1746 2085
Size: 1746 339
HindIII A'AGCT_T
Cuts at: 0 1242 2085
Size: 1242 843
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 1082 2085
Size: 203 879 1003
Fragments arranged by size:
1003 879 203
MscI TGG'CCA
Cuts at: 0 568 852 2085
Size: 568 284 1233
Fragments arranged by size:
1233 568 284
MslI CAynn'nnrTG
Cuts at: 0 442 781 1144 1168 1798 2085
Size: 442 339 363 24 630 287
Fragments arranged by size:
630 442 363 339 287 24
MspA1I CmG'CkG
Cuts at: 0 259 1130 2029 2085
Size: 259 871 899 56
Fragments arranged by size:
899 871 259 56
NsiI A_TGCA'T
Cuts at: 0 861 2040 2085
Size: 861 1179 45
Fragments arranged by size:
1179 861 45
NspI r_CATG'y
Cuts at: 0 233 269 1314 1416 2085
Size: 233 36 1045 102 669
Fragments arranged by size:
1045 669 233 102 36
PciI A'CATG_T
Cuts at: 0 229 265 1412 2085
Size: 229 36 1147 673
Fragments arranged by size:
1147 673 229 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 1277 1364 2056 2085
Size: 1277 87 692 29
Fragments arranged by size:
1277 692 87 29
PmlI CAC'GTG
Cuts at: 0 1320 2085
Size: 1320 765
PpuMI rG'GwC_Cy
Cuts at: 0 160 2085
Size: 160 1925
PstI C_TGCA'G
Cuts at: 0 187 2085
Size: 187 1898
PvuII CAG'CTG
Cuts at: 0 259 2029 2085
Size: 259 1770 56
Fragments arranged by size:
1770 259 56
SacI G_AGCT'C
Cuts at: 0 372 2085
Size: 372 1713
SapI GCTCTTCn'nnn_
Cuts at: 0 544 1480 2085
Size: 544 936 605
Fragments arranged by size:
936 605 544
SfcI C'TryA_G
Cuts at: 0 69 183 326 512 555 1595 2085
Size: 69 114 143 186 43 1040 490
Fragments arranged by size:
1040 490 186 143 114 69 43
SmaI CCC'GGG
Cuts at: 0 1285 2085
Size: 1285 800
SmlI C'TyrA_G
Cuts at: 0 395 536 2085
Size: 395 141 1549
Fragments arranged by size:
1549 395 141
SspI AAT'ATT
Cuts at: 0 477 507 1505 2085
Size: 477 30 998 580
Fragments arranged by size:
998 580 477 30
StuI AGG'CCT
Cuts at: 0 1642 2085
Size: 1642 443
StyI C'CwwG_G
Cuts at: 0 890 2085
Size: 890 1195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1204 1559 2085
Size: 1204 355 526
Fragments arranged by size:
1204 526 355
TatI w'GTAC_w
Cuts at: 0 268 2085
Size: 268 1817
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 58 90 2085
Size: 58 32 1995
Fragments arranged by size:
1995 58 32
XbaI T'CTAG_A
Cuts at: 0 357 2085
Size: 357 1728
XhoI C'TCGA_G
Cuts at: 0 536 2085
Size: 536 1549
XmaI C'CCGG_G
Cuts at: 0 1283 2085
Size: 1283 802
Enzymes that do cut:
AarI AcuI AflIII AleI ApoI AseI AvaI BamHI
BanI BanII BbsI BclI BfrBI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BseYI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI BstBI
BstXI BstYI Bsu36I BtsI ClaI CspCI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I HincII
HindIII MmeI MscI MslI MspA1I NsiI NspI PciI
PflMI PmlI PpuMI PstI PvuII SacI SapI SfcI
SmaI SmlI SspI StuI StyI TaqII TatI TstI
XbaI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BglII BmgBI BmtI
BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI
BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstEII
BstZ17I BtgI BtgZI DraI DrdI EagI EciI EcoNI
EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SwaI Tth111I XcmI XmnI
ZraI