Restriction map for
pFN21AE9455
(Linear) (Six-base) MAPSORT of: KIEE9455.seq Check: 5406 from: 1 to: 1584
KIEE9455.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 93 1584
Size: 93 1491
AccI GT'mk_AC
Cuts at: 0 1037 1139 1584
Size: 1037 102 445
Fragments arranged by size:
1037 445 102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 1545 1584
Size: 301 1244 39
Fragments arranged by size:
1244 301 39
AflIII A'CryG_T
Cuts at: 0 1199 1584
Size: 1199 385
AleI CACnn'nnGTG
Cuts at: 0 11 1584
Size: 11 1573
AlwNI CAG_nnn'CTG
Cuts at: 0 225 527 1355 1584
Size: 225 302 828 229
Fragments arranged by size:
828 302 229 225
ApaI G_GGCC'C
Cuts at: 0 474 1095 1584
Size: 474 621 489
Fragments arranged by size:
621 489 474
ApaLI G'TGCA_C
Cuts at: 0 1231 1584
Size: 1231 353
AvaI C'yCGr_G
Cuts at: 0 314 353 928 1027 1584
Size: 314 39 575 99 557
Fragments arranged by size:
575 557 314 99 39
BamHI G'GATC_C
Cuts at: 0 507 1584
Size: 507 1077
BanI G'GyrC_C
Cuts at: 0 13 288 735 1520 1584
Size: 13 275 447 785 64
Fragments arranged by size:
785 447 275 64 13
BanII G_rGCy'C
Cuts at: 0 124 474 616 904 1095 1135 1225 1584
Size: 124 350 142 288 191 40 90 359
Fragments arranged by size:
359 350 288 191 142 124 90 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 561 1155 1584
Size: 561 594 429
Fragments arranged by size:
594 561 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 759 793 1584
Size: 759 34 791
Fragments arranged by size:
791 759 34
BclI T'GATC_A
Cuts at: 0 791 1584
Size: 791 793
BglI GCCn_nnn'nGGC
Cuts at: 0 548 620 1584
Size: 548 72 964
Fragments arranged by size:
964 548 72
BlpI GC'TnA_GC
Cuts at: 0 238 898 976 1584
Size: 238 660 78 608
Fragments arranged by size:
660 608 238 78
Bme1580I G_kGCm'C
Cuts at: 0 18 293 474 491 929 1095 1235 1584
Size: 18 275 181 17 438 166 140 349
Fragments arranged by size:
438 349 275 181 166 140 18 17
BmgBI CAC'GTC
Cuts at: 0 1268 1584
Size: 1268 316
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 657 1584
Size: 657 927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 630 1584
Size: 175 455 954
Fragments arranged by size:
954 455 175
Bpu10I CC'TnA_GC
Cuts at: 0 82 1584
Size: 82 1502
BsaAI yAC'GTr
Cuts at: 0 492 746 1584
Size: 492 254 838
Fragments arranged by size:
838 492 254
BsaBI GATnn'nnATC
Cuts at: 0 997 1584
Size: 997 587
BsaHI Gr'CG_yC
Cuts at: 0 359 1584
Size: 359 1225
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1012 1015 1557 1584
Size: 1012 3 542 27
Fragments arranged by size:
1012 542 27 3
BseYI C'CCAG_C
Cuts at: 0 473 708 1085 1095 1172 1211 1377 1395
Size: 473 235 377 10 77 39 166 18
Cuts at: 1395 1448 1514 1584
Size: 53 66 70
Fragments arranged by size:
473 377 235 166 77 70 66 53
39 18 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 946 1134 1584
Size: 790 156 188 450
Fragments arranged by size:
790 450 188 156
BsiHKAI G_wGCw'C
Cuts at: 0 124 659 1135 1235 1584
Size: 124 535 476 100 349
Fragments arranged by size:
535 476 349 124 100
BsmI GAATG_Cn'
Cuts at: 0 57 907 1584
Size: 57 850 677
Fragments arranged by size:
850 677 57
Bsp1286I G_dGCh'C
Cuts at: 0 18 124 293 474 491 616 659 904
Size: 18 106 169 181 17 125 43 245
Cuts at: 904 929 1095 1135 1225 1235 1584
Size: 25 166 40 90 10 349
Fragments arranged by size:
349 245 181 169 166 125 106 90
43 40 25 18 17 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 93 866 1584
Size: 93 773 718
Fragments arranged by size:
773 718 93
BsrBI CCG'CTC
Cuts at: 0 1329 1526 1584
Size: 1329 197 58
Fragments arranged by size:
1329 197 58
BsrDI GCAATG_nn'
Cuts at: 0 920 1584
Size: 920 664
BssSI C'ACGA_G
Cuts at: 0 757 1584
Size: 757 827
BstAPI GCAn_nnn'nTGC
Cuts at: 0 330 1584
Size: 330 1254
BstYI r'GATC_y
Cuts at: 0 507 1584
Size: 507 1077
BstZ17I GTA'TAC
Cuts at: 0 1140 1584
Size: 1140 444
Bsu36I CC'TnA_GG
Cuts at: 0 502 617 1584
Size: 502 115 967
Fragments arranged by size:
967 502 115
BtgI C'CryG_G
Cuts at: 0 1226 1584
Size: 1226 358
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 870 1584
Size: 870 714
DraIII CAC_nnn'GTG
Cuts at: 0 1345 1584
Size: 1345 239
EaeI y'GGCC_r
Cuts at: 0 950 995 1584
Size: 950 45 589
Fragments arranged by size:
950 589 45
EarI CTCTTCn'nnn_
Cuts at: 0 1279 1465 1584
Size: 1279 186 119
Fragments arranged by size:
1279 186 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1348 1584
Size: 1348 236
EcoICRI GAG'CTC
Cuts at: 0 122 1133 1584
Size: 122 1011 451
Fragments arranged by size:
1011 451 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 301 630 1545 1584
Size: 175 126 329 915 39
Fragments arranged by size:
915 329 175 126 39
EcoO109I rG'GnC_Cy
Cuts at: 0 470 1091 1092 1352 1584
Size: 470 621 1 260 232
Fragments arranged by size:
621 470 260 232 1
FspI TGC'GCA
Cuts at: 0 1315 1584
Size: 1315 269
HaeII r_GCGC'y
Cuts at: 0 1303 1584
Size: 1303 281
HincII GTy'rAC
Cuts at: 0 142 1584
Size: 142 1442
HindIII A'AGCT_T
Cuts at: 0 1069 1584
Size: 1069 515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1584
Size: 434 1150
MscI TGG'CCA
Cuts at: 0 997 1584
Size: 997 587
MslI CAynn'nnrTG
Cuts at: 0 11 850 991 1584
Size: 11 839 141 593
Fragments arranged by size:
839 593 141 11
MspA1I CmG'CkG
Cuts at: 0 153 477 665 955 980 1172 1452 1518
Size: 153 324 188 290 25 192 280 66
Cuts at: 1518 1584
Size: 66
Fragments arranged by size:
324 290 280 192 188 153 66 66
25
NdeI CA'TA_TG
Cuts at: 0 1207 1584
Size: 1207 377
NspI r_CATG'y
Cuts at: 0 313 865 941 1203 1584
Size: 313 552 76 262 381
Fragments arranged by size:
552 381 313 262 76
PasI CC'CwG_GG
Cuts at: 0 377 599 1584
Size: 377 222 985
Fragments arranged by size:
985 377 222
PciI A'CATG_T
Cuts at: 0 1199 1584
Size: 1199 385
PflMI CCAn_nnn'nTGG
Cuts at: 0 153 225 543 1211 1584
Size: 153 72 318 668 373
Fragments arranged by size:
668 373 318 153 72
PmlI CAC'GTG
Cuts at: 0 492 1584
Size: 492 1092
PshAI GACnn'nnGTC
Cuts at: 0 565 1584
Size: 565 1019
PspOMI G'GGCC_C
Cuts at: 0 470 1091 1584
Size: 470 621 493
Fragments arranged by size:
621 493 470
PstI C_TGCA'G
Cuts at: 0 726 769 1584
Size: 726 43 815
Fragments arranged by size:
815 726 43
PvuII CAG'CTG
Cuts at: 0 153 477 980 1172 1452 1584
Size: 153 324 503 192 280 132
Fragments arranged by size:
503 324 280 192 153 132
SacI G_AGCT'C
Cuts at: 0 124 1135 1584
Size: 124 1011 449
Fragments arranged by size:
1011 449 124
SexAI A'CCwGG_T
Cuts at: 0 1280 1584
Size: 1280 304
SfcI C'TryA_G
Cuts at: 0 722 765 1584
Size: 722 43 819
Fragments arranged by size:
819 722 43
SmaI CCC'GGG
Cuts at: 0 316 355 930 1584
Size: 316 39 575 654
Fragments arranged by size:
654 575 316 39
SnaBI TAC'GTA
Cuts at: 0 746 1584
Size: 746 838
SphI G_CATG'C
Cuts at: 0 313 865 941 1584
Size: 313 552 76 643
Fragments arranged by size:
643 552 313 76
StuI AGG'CCT
Cuts at: 0 1558 1584
Size: 1558 26
StyI C'CwwG_G
Cuts at: 0 1412 1541 1584
Size: 1412 129 43
Fragments arranged by size:
1412 129 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 225 430 1203 1584
Size: 225 205 773 381
Fragments arranged by size:
773 381 225 205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 200 232 813 845 1584
Size: 200 32 581 32 739
Fragments arranged by size:
739 581 200 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1366 1584
Size: 1366 218
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 222 481 1410 1584
Size: 222 259 929 174
Fragments arranged by size:
929 259 222 174
XmaI C'CCGG_G
Cuts at: 0 314 353 928 1584
Size: 314 39 575 656
Fragments arranged by size:
656 575 314 39
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbsI BcgI BclI BglI
BlpI Bme1580I BmgBI BplI BpmI Bpu10I BsaAI BsaBI
BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrDI BssSI BstAPI BstYI BstZ17I Bsu36I BtgI
BtgZI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I
FspI HaeII HincII HindIII MmeI MscI MslI MspA1I
NdeI NspI PasI PciI PflMI PmlI PshAI PspOMI
PstI PvuII SacI SexAI SfcI SmaI SnaBI SphI
StuI StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BciVI BfrBI BglII BmrI BmtI BpuEI BsaI
BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrFI
BsrGI BssHII BstBI BstEII BstXI BtsI ClaI CspCI
DraI DrdI EagI EcoNI EcoRI EcoRV FalI FseI
FspAI Hin4I HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PfoI PmeI PpiI PpuMI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmlI SpeI SrfI SspI SwaI
TatI XbaI XhoI XmnI ZraI