Restriction map for
pFN21AE9453
(Linear) (Six-base) MAPSORT of: KIEE9453.seq Check: 6189 from: 1 to: 2730
KIEE9453.orf
With 182 enzymes: SgfI *
January 29, 2014 12:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 226 2730
Size: 139 87 2504
Fragments arranged by size:
2504 139 87
AflII C'TTAA_G
Cuts at: 0 2565 2730
Size: 2565 165
AflIII A'CryG_T
Cuts at: 0 684 1864 2730
Size: 684 1180 866
Fragments arranged by size:
1180 866 684
AleI CACnn'nnGTG
Cuts at: 0 1240 1768 1948 2730
Size: 1240 528 180 782
Fragments arranged by size:
1240 782 528 180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1258 2730
Size: 1258 1472
AlwNI CAG_nnn'CTG
Cuts at: 0 150 1978 2448 2730
Size: 150 1828 470 282
Fragments arranged by size:
1828 470 282 150
ApoI r'AATT_y
Cuts at: 0 198 501 545 572 927 1266 1279 2623
Size: 198 303 44 27 355 339 13 1344
Cuts at: 2623 2730
Size: 107
Fragments arranged by size:
1344 355 339 303 198 107 44 27
13
AseI AT'TA_AT
Cuts at: 0 473 2730
Size: 473 2257
BanI G'GyrC_C
Cuts at: 0 1242 1930 2123 2730
Size: 1242 688 193 607
Fragments arranged by size:
1242 688 607 193
BanII G_rGCy'C
Cuts at: 0 2405 2730
Size: 2405 325
BbsI GAAGACnn'nnnn_
Cuts at: 0 323 740 2730
Size: 323 417 1990
Fragments arranged by size:
1990 417 323
BbvCI CC'TCA_GC
Cuts at: 0 418 2730
Size: 418 2312
BclI T'GATC_A
Cuts at: 0 1811 2730
Size: 1811 919
BfrBI ATG'CAT
Cuts at: 0 2482 2668 2730
Size: 2482 186 62
Fragments arranged by size:
2482 186 62
BglI GCCn_nnn'nGGC
Cuts at: 0 56 697 958 2730
Size: 56 641 261 1772
Fragments arranged by size:
1772 641 261 56
Bme1580I G_kGCm'C
Cuts at: 0 463 2128 2730
Size: 463 1665 602
Fragments arranged by size:
1665 602 463
BmgBI CAC'GTC
Cuts at: 0 1679 1867 2730
Size: 1679 188 863
Fragments arranged by size:
1679 863 188
BmrI ACTGGGnnnn_n'
Cuts at: 0 124 759 1786 2730
Size: 124 635 1027 944
Fragments arranged by size:
1027 944 635 124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1610 2730
Size: 1610 1120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 633 1982 2328 2702 2730
Size: 401 232 1349 346 374 28
Fragments arranged by size:
1349 401 374 346 232 28
Bpu10I CC'TnA_GC
Cuts at: 0 418 1496 1585 2730
Size: 418 1078 89 1145
Fragments arranged by size:
1145 1078 418 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1649 2730
Size: 1649 1081
BsaWI w'CCGG_w
Cuts at: 0 2051 2730
Size: 2051 679
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1684 1714 2730
Size: 1684 30 1016
Fragments arranged by size:
1684 1016 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2368 2730
Size: 2368 362
BseYI C'CCAG_C
Cuts at: 0 628 2081 2730
Size: 628 1453 649
Fragments arranged by size:
1453 649 628
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1953 2234 2730
Size: 1953 281 496
Fragments arranged by size:
1953 496 281
BsiHKAI G_wGCw'C
Cuts at: 0 387 902 2730
Size: 387 515 1828
Fragments arranged by size:
1828 515 387
BsmI GAATG_Cn'
Cuts at: 0 2482 2730
Size: 2482 248
Bsp1286I G_dGCh'C
Cuts at: 0 387 463 902 2128 2405 2730
Size: 387 76 439 1226 277 325
Fragments arranged by size:
1226 439 387 325 277 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 867 2730
Size: 867 1863
BsrDI GCAATG_nn'
Cuts at: 0 954 2150 2730
Size: 954 1196 580
Fragments arranged by size:
1196 954 580
BssSI C'ACGA_G
Cuts at: 0 2062 2730
Size: 2062 668
BstBI TT'CG_AA
Cuts at: 0 609 2730
Size: 609 2121
BstEII G'GTnAC_C
Cuts at: 0 159 597 2730
Size: 159 438 2133
Fragments arranged by size:
2133 438 159
BstXI CCAn_nnnn'nTGG
Cuts at: 0 230 2730
Size: 230 2500
BstYI r'GATC_y
Cuts at: 0 1417 2730
Size: 1417 1313
BtgI C'CryG_G
Cuts at: 0 51 1520 1946 2730
Size: 51 1469 426 784
Fragments arranged by size:
1469 784 426 51
BtsI GCAGTG_nn'
Cuts at: 0 310 660 834 1633 2644 2730
Size: 310 350 174 799 1011 86
Fragments arranged by size:
1011 799 350 310 174 86
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1004 1039 2730
Size: 1004 35 1691
Fragments arranged by size:
1691 1004 35
DraIII CAC_nnn'GTG
Cuts at: 0 1653 2730
Size: 1653 1077
EaeI y'GGCC_r
Cuts at: 0 48 1079 2730
Size: 48 1031 1651
Fragments arranged by size:
1651 1031 48
EarI CTCTTCn'nnn_
Cuts at: 0 1262 2365 2730
Size: 1262 1103 365
Fragments arranged by size:
1262 1103 365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 226 401 633 1982 2328 2702 2730
Size: 139 87 175 232 1349 346 374 28
Fragments arranged by size:
1349 374 346 232 175 139 87 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2181 2396 2730
Size: 2181 215 334
Fragments arranged by size:
2181 334 215
EcoRI G'AATT_C
Cuts at: 0 501 572 2730
Size: 501 71 2158
Fragments arranged by size:
2158 501 71
EcoRV GAT'ATC
Cuts at: 0 1517 2730
Size: 1517 1213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1766 1798 2483 2515 2730
Size: 1766 32 685 32 215
Fragments arranged by size:
1766 685 215 32 32
HincII GTy'rAC
Cuts at: 0 1454 2730
Size: 1454 1276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1273 2082 2717 2730
Size: 1273 809 635 13
Fragments arranged by size:
1273 809 635 13
MscI TGG'CCA
Cuts at: 0 1081 2730
Size: 1081 1649
MslI CAynn'nnrTG
Cuts at: 0 228 1240 1714 1768 1948 2062 2362 2730
Size: 228 1012 474 54 180 114 300 368
Fragments arranged by size:
1012 474 368 300 228 180 114 54
MspA1I CmG'CkG
Cuts at: 0 147 964 1975 2730
Size: 147 817 1011 755
Fragments arranged by size:
1011 817 755 147
NdeI CA'TA_TG
Cuts at: 0 564 2730
Size: 564 2166
NsiI A_TGCA'T
Cuts at: 0 2484 2670 2730
Size: 2484 186 60
Fragments arranged by size:
2484 186 60
NspI r_CATG'y
Cuts at: 0 688 2461 2730
Size: 688 1773 269
Fragments arranged by size:
1773 688 269
PciI A'CATG_T
Cuts at: 0 684 2730
Size: 684 2046
PflMI CCAn_nnn'nTGG
Cuts at: 0 2076 2448 2730
Size: 2076 372 282
Fragments arranged by size:
2076 372 282
PstI C_TGCA'G
Cuts at: 0 305 826 2730
Size: 305 521 1904
Fragments arranged by size:
1904 521 305
PvuII CAG'CTG
Cuts at: 0 147 964 1975 2730
Size: 147 817 1011 755
Fragments arranged by size:
1011 817 755 147
SapI GCTCTTCn'nnn_
Cuts at: 0 1262 2365 2730
Size: 1262 1103 365
Fragments arranged by size:
1262 1103 365
SexAI A'CCwGG_T
Cuts at: 0 328 2730
Size: 328 2402
SfcI C'TryA_G
Cuts at: 0 165 301 822 834 2730
Size: 165 136 521 12 1896
Fragments arranged by size:
1896 521 165 136 12
SmlI C'TyrA_G
Cuts at: 0 1664 2565 2730
Size: 1664 901 165
Fragments arranged by size:
1664 901 165
SspI AAT'ATT
Cuts at: 0 1062 2730
Size: 1062 1668
StyI C'CwwG_G
Cuts at: 0 176 797 2730
Size: 176 621 1933
Fragments arranged by size:
1933 621 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 616 860 2111 2730
Size: 616 244 1251 619
Fragments arranged by size:
1251 619 616 244
TatI w'GTAC_w
Cuts at: 0 1687 2730
Size: 1687 1043
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 318 350 1684 1712 1716 1744 2417 2449
Size: 318 32 1334 28 4 28 673 32
Cuts at: 2449 2730
Size: 281
Fragments arranged by size:
1334 673 318 281 32 32 28 28
4
Tth111I GACn'n_nGTC
Cuts at: 0 778 2333 2730
Size: 778 1555 397
Fragments arranged by size:
1555 778 397
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 610 1107 2361 2730
Size: 610 497 1254 369
Fragments arranged by size:
1254 610 497 369
XmnI GAAnn'nnTTC
Cuts at: 0 572 2730
Size: 572 2158
Enzymes that do cut:
AcuI AflII AflIII AleI AlfI AlwNI ApoI AseI
BanI BanII BbsI BbvCI BclI BfrBI BglI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BssSI BstBI BstEII BstXI BstYI BtgI BtsI CspCI
DraIII EaeI EarI Eco57MI EcoNI EcoRI EcoRV Hin4I
HincII MmeI MscI MslI MspA1I NdeI NsiI NspI
PciI PflMI PstI PvuII SapI SexAI SfcI SmlI
SspI StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BbeI BcgI BciVI BglII BlpI BmtI BsaI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BstAPI BstZ17I Bsu36I BtgZI
ClaI DraI DrdI EagI EciI EcoICRI EcoO109I FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
XbaI XhoI XmaI ZraI