Restriction map for
pFN21AE9452
(Linear) (Six-base) MAPSORT of: KIEE9452.seq Check: 3814 from: 1 to: 1956
KIEE9452.orf
With 182 enzymes: SgfI *
January 29, 2014 12:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 139 1956
Size: 139 1817
AccI GT'mk_AC
Cuts at: 0 914 1790 1956
Size: 914 876 166
Fragments arranged by size:
914 876 166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 1956
Size: 632 1324
AflIII A'CryG_T
Cuts at: 0 190 836 972 1956
Size: 190 646 136 984
Fragments arranged by size:
984 646 190 136
AleI CACnn'nnGTG
Cuts at: 0 1081 1956
Size: 1081 875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1186 1956
Size: 1186 770
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 533 565 1956
Size: 533 32 1391
Fragments arranged by size:
1391 533 32
ApaLI G'TGCA_C
Cuts at: 0 1324 1956
Size: 1324 632
ApoI r'AATT_y
Cuts at: 0 412 1554 1956
Size: 412 1142 402
Fragments arranged by size:
1142 412 402
AseI AT'TA_AT
Cuts at: 0 203 1956
Size: 203 1753
BamHI G'GATC_C
Cuts at: 0 1056 1956
Size: 1056 900
BbsI GAAGACnn'nnnn_
Cuts at: 0 897 1956
Size: 897 1059
BbvCI CC'TCA_GC
Cuts at: 0 1801 1956
Size: 1801 155
BglI GCCn_nnn'nGGC
Cuts at: 0 1769 1956
Size: 1769 187
BlpI GC'TnA_GC
Cuts at: 0 40 1090 1667 1956
Size: 40 1050 577 289
Fragments arranged by size:
1050 577 289 40
Bme1580I G_kGCm'C
Cuts at: 0 94 1328 1956
Size: 94 1234 628
Fragments arranged by size:
1234 628 94
BmrI ACTGGGnnnn_n'
Cuts at: 0 695 884 1399 1525 1907 1956
Size: 695 189 515 126 382 49
Fragments arranged by size:
695 515 382 189 126 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1115 1956
Size: 1115 841
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1336 1956
Size: 1336 620
Bpu10I CC'TnA_GC
Cuts at: 0 1801 1956
Size: 1801 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 1353 1956
Size: 572 781 603
Fragments arranged by size:
781 603 572
BsaAI yAC'GTr
Cuts at: 0 876 975 1083 1956
Size: 876 99 108 873
Fragments arranged by size:
876 873 108 99
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 346 376 1956
Size: 346 30 1580
Fragments arranged by size:
1580 346 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 354 1066 1102 1201 1789 1956
Size: 354 712 36 99 588 167
Fragments arranged by size:
712 588 354 167 99 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 1325 1671 1956
Size: 111 1214 346 285
Fragments arranged by size:
1214 346 285 111
BsiHKAI G_wGCw'C
Cuts at: 0 928 1258 1328 1956
Size: 928 330 70 628
Fragments arranged by size:
928 628 330 70
BsmI GAATG_Cn'
Cuts at: 0 306 1956
Size: 306 1650
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1101 1956
Size: 1101 855
Bsp1286I G_dGCh'C
Cuts at: 0 94 928 1258 1328 1956
Size: 94 834 330 70 628
Fragments arranged by size:
834 628 330 94 70
BspHI T'CATG_A
Cuts at: 0 1385 1956
Size: 1385 571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 139 1800 1956
Size: 139 1661 156
Fragments arranged by size:
1661 156 139
BsrDI GCAATG_nn'
Cuts at: 0 10 707 1956
Size: 10 697 1249
Fragments arranged by size:
1249 697 10
BsrFI r'CCGG_y
Cuts at: 0 1267 1956
Size: 1267 689
BsrGI T'GTAC_A
Cuts at: 0 211 1956
Size: 211 1745
BssSI C'ACGA_G
Cuts at: 0 1219 1956
Size: 1219 737
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1893 1956
Size: 1893 63
BstYI r'GATC_y
Cuts at: 0 154 534 1056 1956
Size: 154 380 522 900
Fragments arranged by size:
900 522 380 154
BtgI C'CryG_G
Cuts at: 0 138 1956
Size: 138 1818
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1248 1956
Size: 1248 708
BtsI GCAGTG_nn'
Cuts at: 0 123 675 1168 1337 1956
Size: 123 552 493 169 619
Fragments arranged by size:
619 552 493 169 123
ClaI AT'CG_AT
Cuts at: 0 1482 1956
Size: 1482 474
DraIII CAC_nnn'GTG
Cuts at: 0 995 1694 1956
Size: 995 699 262
Fragments arranged by size:
995 699 262
EaeI y'GGCC_r
Cuts at: 0 1292 1436 1956
Size: 1292 144 520
Fragments arranged by size:
1292 520 144
EarI CTCTTCn'nnn_
Cuts at: 0 758 916 1201 1219 1336 1678 1750 1939
Size: 758 158 285 18 117 342 72 189
Cuts at: 1939 1956
Size: 17
Fragments arranged by size:
758 342 285 189 158 117 72 18
17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 1336 1956
Size: 632 704 620
Fragments arranged by size:
704 632 620
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1501 1956
Size: 1501 455
EcoO109I rG'GnC_Cy
Cuts at: 0 697 1897 1956
Size: 697 1200 59
Fragments arranged by size:
1200 697 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 452 484 490 522 592 624
Size: 346 32 74 32 6 32 70 32
Cuts at: 624 1475 1507 1956
Size: 851 32 449
Fragments arranged by size:
851 449 346 74 70 32 32 32
32 32 6
HincII GTy'rAC
Cuts at: 0 1362 1625 1956
Size: 1362 263 331
Fragments arranged by size:
1362 331 263
MfeI C'AATT_G
Cuts at: 0 603 1956
Size: 603 1353
MscI TGG'CCA
Cuts at: 0 1438 1956
Size: 1438 518
MslI CAynn'nnrTG
Cuts at: 0 1081 1306 1466 1642 1956
Size: 1081 225 160 176 314
Fragments arranged by size:
1081 314 225 176 160
MspA1I CmG'CkG
Cuts at: 0 1426 1723 1956
Size: 1426 297 233
Fragments arranged by size:
1426 297 233
NcoI C'CATG_G
Cuts at: 0 138 1956
Size: 138 1818
NspI r_CATG'y
Cuts at: 0 194 1956
Size: 194 1762
PasI CC'CwG_GG
Cuts at: 0 378 1956
Size: 378 1578
PciI A'CATG_T
Cuts at: 0 190 1956
Size: 190 1766
PflMI CCAn_nnn'nTGG
Cuts at: 0 1734 1956
Size: 1734 222
PfoI T'CCnGG_A
Cuts at: 0 494 1932 1956
Size: 494 1438 24
Fragments arranged by size:
1438 494 24
PmlI CAC'GTG
Cuts at: 0 876 975 1956
Size: 876 99 981
Fragments arranged by size:
981 876 99
PpuMI rG'GwC_Cy
Cuts at: 0 697 1897 1956
Size: 697 1200 59
Fragments arranged by size:
1200 697 59
PshAI GACnn'nnGTC
Cuts at: 0 1357 1956
Size: 1357 599
PstI C_TGCA'G
Cuts at: 0 1181 1449 1956
Size: 1181 268 507
Fragments arranged by size:
1181 507 268
PvuII CAG'CTG
Cuts at: 0 1723 1956
Size: 1723 233
SapI GCTCTTCn'nnn_
Cuts at: 0 916 1678 1956
Size: 916 762 278
Fragments arranged by size:
916 762 278
SfcI C'TryA_G
Cuts at: 0 222 1177 1445 1708 1956
Size: 222 955 268 263 248
Fragments arranged by size:
955 268 263 248 222
SgrAI Cr'CCGG_yG
Cuts at: 0 1267 1956
Size: 1267 689
SmlI C'TyrA_G
Cuts at: 0 587 1368 1956
Size: 587 781 588
Fragments arranged by size:
781 588 587
StuI AGG'CCT
Cuts at: 0 26 1956
Size: 26 1930
StyI C'CwwG_G
Cuts at: 0 138 182 1244 1524 1767 1956
Size: 138 44 1062 280 243 189
Fragments arranged by size:
1062 280 243 189 138 44
TatI w'GTAC_w
Cuts at: 0 211 1956
Size: 211 1745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 681 713 1956
Size: 681 32 1243
Fragments arranged by size:
1243 681 32
XbaI T'CTAG_A
Cuts at: 0 516 1956
Size: 516 1440
XmnI GAAnn'nnTTC
Cuts at: 0 169 1599 1956
Size: 169 1430 357
Fragments arranged by size:
1430 357 169
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AloI ApaLI
ApoI AseI BamHI BbsI BbvCI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstXI BstYI BtgI BtgZI BtsI
ClaI DraIII EaeI EarI Eco57MI EcoNI EcoO109I Hin4I
HincII MfeI MscI MslI MspA1I NcoI NspI PasI
PciI PflMI PfoI PmlI PpuMI PshAI PstI PvuII
SapI SfcI SgrAI SmlI StuI StyI TatI TstI
XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlwNI
ApaI AscI AsiSI AvaI AvrII BaeI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
BsaI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BspEI
BsrBI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I CspCI
DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII Tth111I XcmI XhoI
XmaI ZraI