(Linear) (Six-base) MAPSORT of: KIEE9449.seq  Check: 4668  from: 1  to: 1062



KIEE9449.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:29  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     978    1062 
   Size:        978      84
 
AflIII A'CryG_T

Cuts at:      0     167    1062 
   Size:        167     895
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     748    1062 
   Size:        748     314
 
ApaLI G'TGCA_C

Cuts at:      0     546    1062 
   Size:        546     516
 
ApoI r'AATT_y

Cuts at:      0     934    1043    1062 
   Size:        934     109      19

  Fragments arranged by size: 

                 934     109      19
 
BamHI G'GATC_C

Cuts at:      0      63     716    1062 
   Size:         63     653     346

  Fragments arranged by size: 

                 653     346      63
 
BanI G'GyrC_C

Cuts at:      0     867    1062 
   Size:        867     195
 
BanII G_rGCy'C

Cuts at:      0     533    1062 
   Size:        533     529
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     164    1019    1062 
   Size:        164     855      43

  Fragments arranged by size: 

                 855     164      43
 
BglI GCCn_nnn'nGGC

Cuts at:      0     469    1062 
   Size:        469     593
 
Bme1580I G_kGCm'C

Cuts at:      0     550    1062 
   Size:        550     512
 
Bpu10I CC'TnA_GC

Cuts at:      0     527    1062 
   Size:        527     535
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     189    1062 
   Size:        189     873
 
BsaAI yAC'GTr

Cuts at:      0     522     657    1062 
   Size:        522     135     405

  Fragments arranged by size: 

                 522     405     135
 
BsaBI GATnn'nnATC

Cuts at:      0      62    1062 
   Size:         62    1000
 
BseYI C'CCAG_C

Cuts at:      0     293    1062 
   Size:        293     769
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     985    1062 
   Size:        985      77
 
BsiHKAI G_wGCw'C

Cuts at:      0     550    1062 
   Size:        550     512
 
Bsp1286I G_dGCh'C

Cuts at:      0     533     550    1062 
   Size:        533      17     512

  Fragments arranged by size: 

                 533     512      17
 
BspHI T'CATG_A

Cuts at:      0    1047    1062 
   Size:       1047      15
 
BsrDI GCAATG_nn'

Cuts at:      0     665    1062 
   Size:        665     397
 
BsrGI T'GTAC_A

Cuts at:      0     899    1062 
   Size:        899     163
 
BssSI C'ACGA_G

Cuts at:      0     543    1062 
   Size:        543     519
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     889    1062 
   Size:        889     173
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     501     955    1062 
   Size:        501     454     107

  Fragments arranged by size: 

                 501     454     107
 
BstYI r'GATC_y

Cuts at:      0      63     716    1062 
   Size:         63     653     346

  Fragments arranged by size: 

                 653     346      63
 
Bsu36I CC'TnA_GG

Cuts at:      0      26    1062 
   Size:         26    1036
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     775     910    1062 
   Size:        775     135     152

  Fragments arranged by size: 

                 775     152     135
 
BtsI GCAGTG_nn'

Cuts at:      0      14     812    1062 
   Size:         14     798     250

  Fragments arranged by size: 

                 798     250      14
 
ClaI AT'CG_AT

Cuts at:      0     390    1062 
   Size:        390     672
 
EarI CTCTTCn'nnn_

Cuts at:      0     616     690    1046    1062 
   Size:        616      74     356      16

  Fragments arranged by size: 

                 616     356      74      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     978    1062 
   Size:        978      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      25    1062 
   Size:         25    1037
 
HaeII r_GCGC'y

Cuts at:      0      39    1062 
   Size:         39    1023
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     888     920    1062 
   Size:        888      32     142

  Fragments arranged by size: 

                 888     142      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     694    1062 
   Size:        694     368
 
MspA1I CmG'CkG

Cuts at:      0     202     293     320    1062 
   Size:        202      91      27     742

  Fragments arranged by size: 

                 742     202      91      27
 
NspI r_CATG'y

Cuts at:      0     171    1062 
   Size:        171     891
 
PciI A'CATG_T

Cuts at:      0     167    1062 
   Size:        167     895
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     735    1062 
   Size:        735     327
 
PshAI GACnn'nnGTC

Cuts at:      0     376    1062 
   Size:        376     686
 
PstI C_TGCA'G

Cuts at:      0     894    1062 
   Size:        894     168
 
SfcI C'TryA_G

Cuts at:      0     890    1062 
   Size:        890     172
 
SnaBI TAC'GTA

Cuts at:      0     522     657    1062 
   Size:        522     135     405

  Fragments arranged by size: 

                 522     405     135
 
StuI AGG'CCT

Cuts at:      0     574    1062 
   Size:        574     488
 
StyI C'CwwG_G

Cuts at:      0     500     671    1062 
   Size:        500     171     391

  Fragments arranged by size: 

                 500     391     171
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     285    1062 
   Size:        285     777
 
TatI w'GTAC_w

Cuts at:      0     899    1062 
   Size:        899     163
 
XbaI T'CTAG_A

Cuts at:      0     175    1062 
   Size:        175     887
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     498    1062 
   Size:        498     564
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI    ApaLI     ApoI    BamHI     BanI    BanII
     BbsI     BglI Bme1580I   Bpu10I     BsaI    BsaAI    BsaBI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspHI    BsrDI    BsrGI    BssSI   BstAPI
    BstXI    BstYI   Bsu36I    BtgZI     BtsI     ClaI     EarI  Eco57MI
    EcoNI    HaeII    Hin4I     MmeI   MspA1I     NspI     PciI    PflMI
    PshAI     PstI     SfcI    SnaBI     StuI     StyI    TaqII     TatI
     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrFI   BssHII    BstBI   BstEII
  BstZ17I     BtgI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI
     SphI     SrfI     SspI     SwaI     TstI  Tth111I     XhoI     XmaI
     XmnI     ZraI