Restriction map for
pFN21AE9448
(Linear) (Six-base) MAPSORT of: KIEE9448.seq Check: 8725 from: 1 to: 1434
KIEE9448.orf
With 182 enzymes: SgfI *
March 22, 2012 18:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 318 1100 1434
Size: 318 782 334
Fragments arranged by size:
782 334 318
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 759 1434
Size: 759 675
AflIII A'CryG_T
Cuts at: 0 317 972 1434
Size: 317 655 462
Fragments arranged by size:
655 462 317
AlwNI CAG_nnn'CTG
Cuts at: 0 148 861 1092 1279 1332 1434
Size: 148 713 231 187 53 102
Fragments arranged by size:
713 231 187 148 102 53
AvaI C'yCGr_G
Cuts at: 0 640 1434
Size: 640 794
BanI G'GyrC_C
Cuts at: 0 999 1220 1353 1434
Size: 999 221 133 81
Fragments arranged by size:
999 221 133 81
BanII G_rGCy'C
Cuts at: 0 50 453 804 930 997 1240 1434
Size: 50 403 351 126 67 243 194
Fragments arranged by size:
403 351 243 194 126 67 50
BbeI G_GCGC'C
Cuts at: 0 1224 1434
Size: 1224 210
BbsI GAAGACnn'nnnn_
Cuts at: 0 369 1402 1434
Size: 369 1033 32
Fragments arranged by size:
1033 369 32
BbvCI CC'TCA_GC
Cuts at: 0 1198 1434
Size: 1198 236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 418 452 1434
Size: 418 34 982
Fragments arranged by size:
982 418 34
BclI T'GATC_A
Cuts at: 0 473 1434
Size: 473 961
BglI GCCn_nnn'nGGC
Cuts at: 0 952 1434
Size: 952 482
Bme1580I G_kGCm'C
Cuts at: 0 223 641 1434
Size: 223 418 793
Fragments arranged by size:
793 418 223
BmgBI CAC'GTC
Cuts at: 0 975 1434
Size: 975 459
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 621 645 790 1354 1373 1434
Size: 93 528 24 145 564 19 61
Fragments arranged by size:
564 528 145 93 61 24 19
Bpu10I CC'TnA_GC
Cuts at: 0 1198 1434
Size: 1198 236
BsaAI yAC'GTr
Cuts at: 0 636 1434
Size: 636 798
BsaHI Gr'CG_yC
Cuts at: 0 544 1221 1434
Size: 544 677 213
Fragments arranged by size:
677 544 213
BsaWI w'CCGG_w
Cuts at: 0 302 1434
Size: 302 1132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 788 818 1434
Size: 788 30 616
Fragments arranged by size:
788 616 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 898 1434
Size: 898 536
BseYI C'CCAG_C
Cuts at: 0 31 49 66 76 255 573 961 1085
Size: 31 18 17 10 179 318 388 124
Cuts at: 1085 1113 1147 1295 1414 1434
Size: 28 34 148 119 20
Fragments arranged by size:
388 318 179 148 124 119 34 31
28 20 18 17 10
BsiEI CG_ry'CG
Cuts at: 0 692 1434
Size: 692 742
BsiHKAI G_wGCw'C
Cuts at: 0 453 920 997 1434
Size: 453 467 77 437
Fragments arranged by size:
467 453 437 77
Bsp1286I G_dGCh'C
Cuts at: 0 50 223 453 641 804 920 930 997
Size: 50 173 230 188 163 116 10 67
Cuts at: 997 1240 1434
Size: 243 194
Fragments arranged by size:
243 230 194 188 173 163 116 67
50 10
BspEI T'CCGG_A
Cuts at: 0 302 1434
Size: 302 1132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 318 1100 1434
Size: 318 782 334
Fragments arranged by size:
782 334 318
BsrBI CCG'CTC
Cuts at: 0 1157 1169 1434
Size: 1157 12 265
Fragments arranged by size:
1157 265 12
BsrDI GCAATG_nn'
Cuts at: 0 837 1079 1434
Size: 837 242 355
Fragments arranged by size:
837 355 242
BsrFI r'CCGG_y
Cuts at: 0 119 687 1127 1434
Size: 119 568 440 307
Fragments arranged by size:
568 440 307 119
BstAPI GCAn_nnn'nTGC
Cuts at: 0 861 1434
Size: 861 573
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 80 495 1434
Size: 57 23 415 939
Fragments arranged by size:
939 415 57 23
BstYI r'GATC_y
Cuts at: 0 1205 1434
Size: 1205 229
BtgI C'CryG_G
Cuts at: 0 182 1434
Size: 182 1252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 474 996 1434
Size: 474 522 438
Fragments arranged by size:
522 474 438
BtsI GCAGTG_nn'
Cuts at: 0 409 1434
Size: 409 1025
EaeI y'GGCC_r
Cuts at: 0 59 485 527 689 881 1076 1434
Size: 59 426 42 162 192 195 358
Fragments arranged by size:
426 358 195 192 162 59 42
EagI C'GGCC_G
Cuts at: 0 689 1434
Size: 689 745
EarI CTCTTCn'nnn_
Cuts at: 0 916 1434
Size: 916 518
EcoICRI GAG'CTC
Cuts at: 0 451 995 1434
Size: 451 544 439
Fragments arranged by size:
544 451 439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 621 645 759 790 1354 1373 1434
Size: 93 528 24 114 31 564 19 61
Fragments arranged by size:
564 528 114 93 61 31 24 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 285 1434
Size: 285 1149
EcoO109I rG'GnC_Cy
Cuts at: 0 171 369 818 1422 1434
Size: 171 198 449 604 12
Fragments arranged by size:
604 449 198 171 12
HaeII r_GCGC'y
Cuts at: 0 632 1224 1434
Size: 632 592 210
Fragments arranged by size:
632 592 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 788 820 1434
Size: 788 32 614
Fragments arranged by size:
788 614 32
KasI G'GCGC_C
Cuts at: 0 1220 1434
Size: 1220 214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1217 1434
Size: 1217 217
MscI TGG'CCA
Cuts at: 0 61 529 883 1078 1434
Size: 61 468 354 195 356
Fragments arranged by size:
468 356 354 195 61
MslI CAynn'nnrTG
Cuts at: 0 250 1434
Size: 250 1184
MspA1I CmG'CkG
Cuts at: 0 53 80 525 852 858 940 1202 1279
Size: 53 27 445 327 6 82 262 77
Cuts at: 1279 1434
Size: 155
Fragments arranged by size:
445 327 262 155 82 77 53 27
6
NaeI GCC'GGC
Cuts at: 0 121 689 1129 1434
Size: 121 568 440 305
Fragments arranged by size:
568 440 305 121
NarI GG'CG_CC
Cuts at: 0 1221 1434
Size: 1221 213
NgoMIV G'CCGG_C
Cuts at: 0 119 687 1127 1434
Size: 119 568 440 307
Fragments arranged by size:
568 440 307 119
NspI r_CATG'y
Cuts at: 0 321 1434
Size: 321 1113
PasI CC'CwG_GG
Cuts at: 0 216 1233 1368 1434
Size: 216 1017 135 66
Fragments arranged by size:
1017 216 135 66
PciI A'CATG_T
Cuts at: 0 317 1434
Size: 317 1117
PflMI CCAn_nnn'nTGG
Cuts at: 0 83 1434
Size: 83 1351
PfoI T'CCnGG_A
Cuts at: 0 785 1434
Size: 785 649
PpuMI rG'GwC_Cy
Cuts at: 0 171 1422 1434
Size: 171 1251 12
Fragments arranged by size:
1251 171 12
PstI C_TGCA'G
Cuts at: 0 289 866 1434
Size: 289 577 568
Fragments arranged by size:
577 568 289
PvuII CAG'CTG
Cuts at: 0 53 80 1202 1279 1434
Size: 53 27 1122 77 155
Fragments arranged by size:
1122 155 77 53 27
SacI G_AGCT'C
Cuts at: 0 453 997 1434
Size: 453 544 437
Fragments arranged by size:
544 453 437
SanDI GG'GwC_CC
Cuts at: 0 171 1434
Size: 171 1263
SexAI A'CCwGG_T
Cuts at: 0 506 1434
Size: 506 928
SfcI C'TryA_G
Cuts at: 0 285 862 1434
Size: 285 577 572
Fragments arranged by size:
577 572 285
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 952 1434
Size: 952 482
SfoI GGC'GCC
Cuts at: 0 1222 1434
Size: 1222 212
StuI AGG'CCT
Cuts at: 0 1021 1434
Size: 1021 413
StyI C'CwwG_G
Cuts at: 0 1016 1349 1400 1434
Size: 1016 333 51 34
Fragments arranged by size:
1016 333 51 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 90 379 1072 1105 1177 1434
Size: 90 289 693 33 72 257
Fragments arranged by size:
693 289 257 90 72 33
TatI w'GTAC_w
Cuts at: 0 1067 1434
Size: 1067 367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 80 537 1071 1303 1434
Size: 80 457 534 232 131
Fragments arranged by size:
534 457 232 131 80
Enzymes that do cut:
AarI AcuI AflIII AlwNI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI Bme1580I BmgBI BpmI
Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI
BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PpuMI PstI PvuII SacI SanDI
SexAI SfcI SfiI SfoI StuI StyI TaqII TatI
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BciVI BfrBI BglII BlpI
BmrI BmtI BplI BpuEI BsaI BsaBI BsgI BsiWI
BsmI BsmBI BspHI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI