Restriction map for
pFN21AE9447
(Linear) (Six-base) MAPSORT of: KIEE9447.seq Check: 8467 from: 1 to: 1206
KIEE9447.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AccI GT'mk_AC
Cuts at: 0 908 1206
Size: 908 298
AclI AA'CG_TT
Cuts at: 0 414 1206
Size: 414 792
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 697 1206
Size: 90 607 509
Fragments arranged by size:
607 509 90
AleI CACnn'nnGTG
Cuts at: 0 498 1206
Size: 498 708
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 193 1206
Size: 193 1013
AlwNI CAG_nnn'CTG
Cuts at: 0 969 1021 1206
Size: 969 52 185
Fragments arranged by size:
969 185 52
AvaI C'yCGr_G
Cuts at: 0 6 1206
Size: 6 1200
BanI G'GyrC_C
Cuts at: 0 72 115 636 989 1206
Size: 72 43 521 353 217
Fragments arranged by size:
521 353 217 72 43
BanII G_rGCy'C
Cuts at: 0 94 148 1206
Size: 94 54 1058
Fragments arranged by size:
1058 94 54
BbeI G_GCGC'C
Cuts at: 0 76 1206
Size: 76 1130
BbsI GAAGACnn'nnnn_
Cuts at: 0 921 1206
Size: 921 285
BbvCI CC'TCA_GC
Cuts at: 0 62 253 985 1206
Size: 62 191 732 221
Fragments arranged by size:
732 221 191 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 351 385 1206
Size: 351 34 821
Fragments arranged by size:
821 351 34
BclI T'GATC_A
Cuts at: 0 443 728 935 1206
Size: 443 285 207 271
Fragments arranged by size:
443 285 271 207
BglI GCCn_nnn'nGGC
Cuts at: 0 466 1206
Size: 466 740
BglII A'GATC_T
Cuts at: 0 708 1206
Size: 708 498
Bme1580I G_kGCm'C
Cuts at: 0 641 1206
Size: 641 565
BmrI ACTGGGnnnn_n'
Cuts at: 0 45 1206
Size: 45 1161
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 532 1206
Size: 532 674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 1206
Size: 483 723
Bpu10I CC'TnA_GC
Cuts at: 0 62 253 985 1206
Size: 62 191 732 221
Fragments arranged by size:
732 221 191 62
BsaHI Gr'CG_yC
Cuts at: 0 73 164 1206
Size: 73 91 1042
Fragments arranged by size:
1042 91 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 583 613 1206
Size: 583 30 593
Fragments arranged by size:
593 583 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 1206
Size: 223 983
BseYI C'CCAG_C
Cuts at: 0 141 159 317 1206
Size: 141 18 158 889
Fragments arranged by size:
889 158 141 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 1206
Size: 546 660
BsiHKAI G_wGCw'C
Cuts at: 0 188 513 1206
Size: 188 325 693
Fragments arranged by size:
693 325 188
BsmI GAATG_Cn'
Cuts at: 0 695 1206
Size: 695 511
Bsp1286I G_dGCh'C
Cuts at: 0 94 148 188 513 641 1206
Size: 94 54 40 325 128 565
Fragments arranged by size:
565 325 128 94 54 40
BspHI T'CATG_A
Cuts at: 0 932 1206
Size: 932 274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 185 269 1206
Size: 185 84 937
Fragments arranged by size:
937 185 84
BsrDI GCAATG_nn'
Cuts at: 0 383 657 1206
Size: 383 274 549
Fragments arranged by size:
549 383 274
BsrFI r'CCGG_y
Cuts at: 0 69 75 1206
Size: 69 6 1131
Fragments arranged by size:
1131 69 6
BstBI TT'CG_AA
Cuts at: 0 687 948 1206
Size: 687 261 258
Fragments arranged by size:
687 261 258
BstEII G'GTnAC_C
Cuts at: 0 282 847 1206
Size: 282 565 359
Fragments arranged by size:
565 359 282
BstXI CCAn_nnnn'nTGG
Cuts at: 0 664 1206
Size: 664 542
BstYI r'GATC_y
Cuts at: 0 708 952 1206
Size: 708 244 254
Fragments arranged by size:
708 254 244
BstZ17I GTA'TAC
Cuts at: 0 909 1206
Size: 909 297
Bsu36I CC'TnA_GG
Cuts at: 0 307 1206
Size: 307 899
BtgI C'CryG_G
Cuts at: 0 27 53 332 1206
Size: 27 26 279 874
Fragments arranged by size:
874 279 27 26
DraI TTT'AAA
Cuts at: 0 740 1206
Size: 740 466
EaeI y'GGCC_r
Cuts at: 0 24 666 758 1206
Size: 24 642 92 448
Fragments arranged by size:
642 448 92 24
EarI CTCTTCn'nnn_
Cuts at: 0 109 280 373 1107 1206
Size: 109 171 93 734 99
Fragments arranged by size:
734 171 109 99 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 483 697 1206
Size: 90 393 214 509
Fragments arranged by size:
509 393 214 90
EcoNI CCTnn'n_nnAGG
Cuts at: 0 306 1206
Size: 306 900
HaeII r_GCGC'y
Cuts at: 0 62 76 129 136 1206
Size: 62 14 53 7 1070
Fragments arranged by size:
1070 62 53 14 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 562 594 1206
Size: 562 32 612
Fragments arranged by size:
612 562 32
HincII GTy'rAC
Cuts at: 0 243 1149 1206
Size: 243 906 57
Fragments arranged by size:
906 243 57
KasI G'GCGC_C
Cuts at: 0 72 1206
Size: 72 1134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 886 1002 1206
Size: 781 105 116 204
Fragments arranged by size:
781 204 116 105
MscI TGG'CCA
Cuts at: 0 668 760 1206
Size: 668 92 446
Fragments arranged by size:
668 446 92
MslI CAynn'nnrTG
Cuts at: 0 424 498 662 688 1051 1206
Size: 424 74 164 26 363 155
Fragments arranged by size:
424 363 164 155 74 26
MspA1I CmG'CkG
Cuts at: 0 20 55 210 1021 1206
Size: 20 35 155 811 185
Fragments arranged by size:
811 185 155 35 20
NaeI GCC'GGC
Cuts at: 0 71 77 1206
Size: 71 6 1129
Fragments arranged by size:
1129 71 6
NarI GG'CG_CC
Cuts at: 0 73 1206
Size: 73 1133
NgoMIV G'CCGG_C
Cuts at: 0 69 75 1206
Size: 69 6 1131
Fragments arranged by size:
1131 69 6
PasI CC'CwG_GG
Cuts at: 0 642 1206
Size: 642 564
PflMI CCAn_nnn'nTGG
Cuts at: 0 1021 1206
Size: 1021 185
PfoI T'CCnGG_A
Cuts at: 0 539 1206
Size: 539 667
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 892 924 1178 1206
Size: 892 32 254 28
Fragments arranged by size:
892 254 32 28
PvuII CAG'CTG
Cuts at: 0 1021 1206
Size: 1021 185
SacII CC_GC'GG
Cuts at: 0 56 1206
Size: 56 1150
SexAI A'CCwGG_T
Cuts at: 0 851 1206
Size: 851 355
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 466 1206
Size: 466 740
SfoI GGC'GCC
Cuts at: 0 74 1206
Size: 74 1132
SgrAI Cr'CCGG_yG
Cuts at: 0 75 1206
Size: 75 1131
StuI AGG'CCT
Cuts at: 0 999 1206
Size: 999 207
StyI C'CwwG_G
Cuts at: 0 581 1206
Size: 581 625
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 309 634 1206
Size: 309 325 572
Fragments arranged by size:
572 325 309
TatI w'GTAC_w
Cuts at: 0 2 1206
Size: 2 1204
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 156 1206
Size: 156 1050
XmnI GAAnn'nnTTC
Cuts at: 0 710 1206
Size: 710 496
Enzymes that do cut:
AccI AclI AcuI AleI AlfI AlwNI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BclI BglI BglII
Bme1580I BmrI BplI BpmI Bpu10I BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrFI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
DraI EaeI EarI Eco57MI EcoNI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
PasI PflMI PfoI PsrI PvuII SacII SexAI SfiI
SfoI SgrAI StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BfrBI BlpI BmgBI BmtI BpuEI
BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI
BsrBI BsrGI BssHII BssSI BstAPI BtgZI BtsI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI