(Linear) (Six-base) MAPSORT of: KIEE9446.seq  Check: 7438  from: 1  to: 1131



KIEE9446.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:28  ..

AccI GT'mk_AC

Cuts at:      0     452    1131 
   Size:        452     679
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     981    1131 
   Size:        119     862     150

  Fragments arranged by size: 

                 862     150     119
 
AlwNI CAG_nnn'CTG

Cuts at:      0      54     951    1131 
   Size:         54     897     180

  Fragments arranged by size: 

                 897     180      54
 
ApaI G_GGCC'C

Cuts at:      0     597    1131 
   Size:        597     534
 
AseI AT'TA_AT

Cuts at:      0     317    1131 
   Size:        317     814
 
BanI G'GyrC_C

Cuts at:      0     345    1131 
   Size:        345     786
 
BanII G_rGCy'C

Cuts at:      0     597    1122    1131 
   Size:        597     525       9

  Fragments arranged by size: 

                 597     525       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     987    1131 
   Size:        987     144
 
BbvCI CC'TCA_GC

Cuts at:      0     424    1131 
   Size:        424     707
 
BclI T'GATC_A

Cuts at:      0     545     938    1131 
   Size:        545     393     193

  Fragments arranged by size: 

                 545     393     193
 
Bme1580I G_kGCm'C

Cuts at:      0     597    1131 
   Size:        597     534
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      61    1131 
   Size:         61    1070
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     531    1030    1131 
   Size:        353     178     499     101

  Fragments arranged by size: 

                 499     353     178     101
 
Bpu10I CC'TnA_GC

Cuts at:      0     424    1131 
   Size:        424     707
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     240    1131 
   Size:        240     891
 
BseYI C'CCAG_C

Cuts at:      0     590    1131 
   Size:        590     541
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604     789    1131 
   Size:        604     185     342

  Fragments arranged by size: 

                 604     342     185
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1039    1131 
   Size:       1039      92
 
Bsp1286I G_dGCh'C

Cuts at:      0     597    1122    1131 
   Size:        597     525       9

  Fragments arranged by size: 

                 597     525       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     372     959    1131 
   Size:        372     587     172

  Fragments arranged by size: 

                 587     372     172
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     387    1131 
   Size:        387     744
 
BtsI GCAGTG_nn'

Cuts at:      0     592    1131 
   Size:        592     539
 
EaeI y'GGCC_r

Cuts at:      0     269    1099    1131 
   Size:        269     830      32

  Fragments arranged by size: 

                 830     269      32
 
EarI CTCTTCn'nnn_

Cuts at:      0     553    1127    1131 
   Size:        553     574       4

  Fragments arranged by size: 

                 574     553       4
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     353     531     981    1030    1131 
   Size:        119     234     178     450      49     101

  Fragments arranged by size: 

                 450     234     178     119     101      49
 
EcoO109I rG'GnC_Cy

Cuts at:      0     171     492     594    1131 
   Size:        171     321     102     537

  Fragments arranged by size: 

                 537     321     171     102
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     776     808    1131 
   Size:        776      32     323

  Fragments arranged by size: 

                 776     323      32
 
HincII GTy'rAC

Cuts at:      0     300    1131 
   Size:        300     831
 
MfeI C'AATT_G

Cuts at:      0     154    1131 
   Size:        154     977
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     174     596     770     838    1131 
   Size:        174     422     174      68     293

  Fragments arranged by size: 

                 422     293     174     174      68
 
MscI TGG'CCA

Cuts at:      0     271    1101    1131 
   Size:        271     830      30

  Fragments arranged by size: 

                 830     271      30
 
MspA1I CmG'CkG

Cuts at:      0     267    1131 
   Size:        267     864
 
PfoI T'CCnGG_A

Cuts at:      0    1046    1131 
   Size:       1046      85
 
PpuMI rG'GwC_Cy

Cuts at:      0     171     492    1131 
   Size:        171     321     639

  Fragments arranged by size: 

                 639     321     171
 
PspOMI G'GGCC_C

Cuts at:      0     593    1131 
   Size:        593     538
 
PstI C_TGCA'G

Cuts at:      0    1024    1131 
   Size:       1024     107
 
PvuII CAG'CTG

Cuts at:      0     267    1131 
   Size:        267     864
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1127    1131 
   Size:       1127       4
 
SbfI CC_TGCA'GG

Cuts at:      0    1024    1131 
   Size:       1024     107
 
SexAI A'CCwGG_T

Cuts at:      0     353    1131 
   Size:        353     778
 
SfcI C'TryA_G

Cuts at:      0    1020    1131 
   Size:       1020     111
 
StyI C'CwwG_G

Cuts at:      0    1126    1131 
   Size:       1126       5
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     928    1131 
   Size:        928     203
 
TatI w'GTAC_w

Cuts at:      0     653    1131 
   Size:        653     478
 
Tth111I GACn'n_nGTC

Cuts at:      0     567    1131 
   Size:        567     564
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApaI     AseI     BanI    BanII     BbsI
    BbvCI     BclI Bme1580I     BmrI     BpmI   Bpu10I    BseRI    BseYI
     BsgI    BsmBI Bsp1286I    BspMI   BstAPI     BtsI     EaeI     EarI
  Eco57MI EcoO109I    Hin4I   HincII     MfeI     MmeI     MscI   MspA1I
     PfoI    PpuMI   PspOMI     PstI    PvuII     SapI     SbfI    SexAI
     SfcI     StyI    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MluI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI