Restriction map for
pFN21AE9445
(Linear) (Six-base) MAPSORT of: KIEE9445.seq Check: 3056 from: 1 to: 1029
KIEE9445.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 532 1029
Size: 532 497
AccI GT'mk_AC
Cuts at: 0 869 1029
Size: 869 160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 1029
Size: 264 765
AfeI AGC'GCT
Cuts at: 0 421 697 1029
Size: 421 276 332
Fragments arranged by size:
421 332 276
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 217 249 1029
Size: 217 32 780
Fragments arranged by size:
780 217 32
AlwNI CAG_nnn'CTG
Cuts at: 0 903 1029
Size: 903 126
ApaI G_GGCC'C
Cuts at: 0 567 684 939 1029
Size: 567 117 255 90
Fragments arranged by size:
567 255 117 90
AvaI C'yCGr_G
Cuts at: 0 814 1014 1029
Size: 814 200 15
Fragments arranged by size:
814 200 15
BanI G'GyrC_C
Cuts at: 0 455 689 1029
Size: 455 234 340
Fragments arranged by size:
455 340 234
BanII G_rGCy'C
Cuts at: 0 371 567 684 939 1029
Size: 371 196 117 255 90
Fragments arranged by size:
371 255 196 117 90
BbeI G_GCGC'C
Cuts at: 0 459 693 1029
Size: 459 234 336
Fragments arranged by size:
459 336 234
BbsI GAAGACnn'nnnn_
Cuts at: 0 732 783 918 1029
Size: 732 51 135 111
Fragments arranged by size:
732 135 111 51
BglI GCCn_nnn'nGGC
Cuts at: 0 170 179 1029
Size: 170 9 850
Fragments arranged by size:
850 170 9
BlpI GC'TnA_GC
Cuts at: 0 953 1029
Size: 953 76
Bme1580I G_kGCm'C
Cuts at: 0 55 328 567 684 939 1029
Size: 55 273 239 117 255 90
Fragments arranged by size:
273 255 239 117 90 55
BmgBI CAC'GTC
Cuts at: 0 717 1029
Size: 717 312
Bpu10I CC'TnA_GC
Cuts at: 0 61 916 1029
Size: 61 855 113
Fragments arranged by size:
855 113 61
BsaI GGTCTCn'nnnn_
Cuts at: 0 738 1029
Size: 738 291
BsaAI yAC'GTr
Cuts at: 0 669 1029
Size: 669 360
BsaHI Gr'CG_yC
Cuts at: 0 456 690 1029
Size: 456 234 339
Fragments arranged by size:
456 339 234
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 217 247 991 1021 1029
Size: 217 30 744 30 8
Fragments arranged by size:
744 217 30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 1029
Size: 148 881
BseYI C'CCAG_C
Cuts at: 0 957 1029
Size: 957 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 514 649 1029
Size: 232 282 135 380
Fragments arranged by size:
380 282 232 135
BsiEI CG_ry'CG
Cuts at: 0 134 407 482 1029
Size: 134 273 75 547
Fragments arranged by size:
547 273 134 75
Bsp1286I G_dGCh'C
Cuts at: 0 55 328 371 567 684 939 1029
Size: 55 273 43 196 117 255 90
Fragments arranged by size:
273 255 196 117 90 55 43
BspHI T'CATG_A
Cuts at: 0 137 1029
Size: 137 892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 532 1029
Size: 532 497
BsrBI CCG'CTC
Cuts at: 0 633 915 1029
Size: 633 282 114
Fragments arranged by size:
633 282 114
BsrFI r'CCGG_y
Cuts at: 0 385 402 458 1029
Size: 385 17 56 571
Fragments arranged by size:
571 385 56 17
BstAPI GCAn_nnn'nTGC
Cuts at: 0 353 1029
Size: 353 676
BstEII G'GTnAC_C
Cuts at: 0 519 1029
Size: 519 510
BstYI r'GATC_y
Cuts at: 0 797 1029
Size: 797 232
BtgI C'CryG_G
Cuts at: 0 482 558 728 995 1029
Size: 482 76 170 267 34
Fragments arranged by size:
482 267 170 76 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 130 1029
Size: 130 899
BtsI GCAGTG_nn'
Cuts at: 0 1012 1029
Size: 1012 17
EaeI y'GGCC_r
Cuts at: 0 131 254 404 479 725 878 1029
Size: 131 123 150 75 246 153 151
Fragments arranged by size:
246 153 151 150 131 123 75
EagI C'GGCC_G
Cuts at: 0 131 404 479 1029
Size: 131 273 75 550
Fragments arranged by size:
550 273 131 75
EarI CTCTTCn'nnn_
Cuts at: 0 283 787 1029
Size: 283 504 242
Fragments arranged by size:
504 283 242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 1029
Size: 264 765
EcoNI CCTnn'n_nnAGG
Cuts at: 0 64 1029
Size: 64 965
EcoO109I rG'GnC_Cy
Cuts at: 0 69 360 504 563 564 936 1029
Size: 69 291 144 59 1 372 93
Fragments arranged by size:
372 291 144 93 69 59 1
HaeII r_GCGC'y
Cuts at: 0 263 416 423 459 474 693 699 857
Size: 263 153 7 36 15 219 6 158
Cuts at: 857 953 1029
Size: 96 76
Fragments arranged by size:
263 219 158 153 96 76 36 15
7 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 993 1025 1029
Size: 993 32 4
Fragments arranged by size:
993 32 4
KasI G'GCGC_C
Cuts at: 0 455 689 1029
Size: 455 234 340
Fragments arranged by size:
455 340 234
MscI TGG'CCA
Cuts at: 0 256 727 1029
Size: 256 471 302
Fragments arranged by size:
471 302 256
MslI CAynn'nnrTG
Cuts at: 0 1000 1029
Size: 1000 29
MspA1I CmG'CkG
Cuts at: 0 560 900 933 1029
Size: 560 340 33 96
Fragments arranged by size:
560 340 96 33
NaeI GCC'GGC
Cuts at: 0 404 460 1029
Size: 404 56 569
Fragments arranged by size:
569 404 56
NarI GG'CG_CC
Cuts at: 0 456 690 1029
Size: 456 234 339
Fragments arranged by size:
456 339 234
NcoI C'CATG_G
Cuts at: 0 728 995 1029
Size: 728 267 34
Fragments arranged by size:
728 267 34
NgoMIV G'CCGG_C
Cuts at: 0 402 458 1029
Size: 402 56 571
Fragments arranged by size:
571 402 56
NruI TCG'CGA
Cuts at: 0 115 1029
Size: 115 914
PmlI CAC'GTG
Cuts at: 0 669 1029
Size: 669 360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 1029
Size: 217 32 780
Fragments arranged by size:
780 217 32
PpuMI rG'GwC_Cy
Cuts at: 0 360 504 1029
Size: 360 144 525
Fragments arranged by size:
525 360 144
PspOMI G'GGCC_C
Cuts at: 0 563 680 935 1029
Size: 563 117 255 94
Fragments arranged by size:
563 255 117 94
PstI C_TGCA'G
Cuts at: 0 583 1029
Size: 583 446
SacII CC_GC'GG
Cuts at: 0 561 1029
Size: 561 468
SbfI CC_TGCA'GG
Cuts at: 0 583 1029
Size: 583 446
SfcI C'TryA_G
Cuts at: 0 81 579 1029
Size: 81 498 450
Fragments arranged by size:
498 450 81
SfoI GGC'GCC
Cuts at: 0 457 691 1029
Size: 457 234 338
Fragments arranged by size:
457 338 234
SgrAI Cr'CCGG_yG
Cuts at: 0 458 1029
Size: 458 571
SmaI CCC'GGG
Cuts at: 0 1016 1029
Size: 1016 13
StyI C'CwwG_G
Cuts at: 0 728 995 1029
Size: 728 267 34
Fragments arranged by size:
728 267 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 420 1029
Size: 420 609
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 991 1023 1029
Size: 991 32 6
Fragments arranged by size:
991 32 6
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 264 1029
Size: 264 765
XmaI C'CCGG_G
Cuts at: 0 1014 1029
Size: 1014 15
XmnI GAAnn'nnTTC
Cuts at: 0 229 1029
Size: 229 800
Enzymes that do cut:
AarI AccI AcuI AfeI AloI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstEII
BstYI BtgI BtgZI BtsI EaeI EagI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI PmlI PpiI PpuMI
PspOMI PstI SacII SbfI SfcI SfoI SgrAI SmaI
StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmrI
BmtI BplI BpmI BpuEI BsaBI BsaWI BsiHKAI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BssHII BssSI BstBI
BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI
NheI NotI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SalI SanDI SapI ScaI SexAI SfiI
SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XhoI ZraI