Restriction map for
pFN21AE9443
(Linear) (Six-base) MAPSORT of: KIEE9443.seq Check: 9557 from: 1 to: 4377
KIEE9443.orf
With 182 enzymes: SgfI *
December 5, 2014 13:30 ..
AccI GT'mk_AC
Cuts at: 0 914 2294 2407 4377
Size: 914 1380 113 1970
Fragments arranged by size:
1970 1380 914 113
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1211 1667 1877 3279 3584 4097 4240
Size: 475 736 456 210 1402 305 513 143
Cuts at: 4240 4377
Size: 137
Fragments arranged by size:
1402 736 513 475 456 305 210 143
137
AfeI AGC'GCT
Cuts at: 0 183 4377
Size: 183 4194
AflII C'TTAA_G
Cuts at: 0 1816 4377
Size: 1816 2561
AflIII A'CryG_T
Cuts at: 0 1133 2452 4377
Size: 1133 1319 1925
Fragments arranged by size:
1925 1319 1133
AleI CACnn'nnGTG
Cuts at: 0 1210 2554 3713 4377
Size: 1210 1344 1159 664
Fragments arranged by size:
1344 1210 1159 664
AlwNI CAG_nnn'CTG
Cuts at: 0 1737 3607 4075 4377
Size: 1737 1870 468 302
Fragments arranged by size:
1870 1737 468 302
ApaI G_GGCC'C
Cuts at: 0 1546 4377
Size: 1546 2831
ApaLI G'TGCA_C
Cuts at: 0 3802 4377
Size: 3802 575
ApoI r'AATT_y
Cuts at: 0 506 691 1361 1884 2053 2680 4377
Size: 506 185 670 523 169 627 1697
Fragments arranged by size:
1697 670 627 523 506 185 169
AseI AT'TA_AT
Cuts at: 0 3173 4377
Size: 3173 1204
AvaI C'yCGr_G
Cuts at: 0 153 3841 3967 4377
Size: 153 3688 126 410
Fragments arranged by size:
3688 410 153 126
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2643 2676 4377
Size: 2643 33 1701
Fragments arranged by size:
2643 1701 33
BanI G'GyrC_C
Cuts at: 0 3972 4377
Size: 3972 405
BanII G_rGCy'C
Cuts at: 0 1546 3848 3953 4377
Size: 1546 2302 105 424
Fragments arranged by size:
2302 1546 424 105
BbsI GAAGACnn'nnnn_
Cuts at: 0 648 1804 3127 4181 4377
Size: 648 1156 1323 1054 196
Fragments arranged by size:
1323 1156 1054 648 196
BbvCI CC'TCA_GC
Cuts at: 0 3986 4377
Size: 3986 391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 907 941 4377
Size: 907 34 3436
Fragments arranged by size:
3436 907 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3642 3657 4377
Size: 3642 15 720
Fragments arranged by size:
3642 720 15
BclI T'GATC_A
Cuts at: 0 3029 3785 4377
Size: 3029 756 592
Fragments arranged by size:
3029 756 592
BglII A'GATC_T
Cuts at: 0 141 4377
Size: 141 4236
BlpI GC'TnA_GC
Cuts at: 0 1232 4377
Size: 1232 3145
Bme1580I G_kGCm'C
Cuts at: 0 1546 3806 4377
Size: 1546 2260 571
Fragments arranged by size:
2260 1546 571
BmgBI CAC'GTC
Cuts at: 0 2256 4377
Size: 2256 2121
BmrI ACTGGGnnnn_n'
Cuts at: 0 329 1581 2215 4377
Size: 329 1252 634 2162
Fragments arranged by size:
2162 1252 634 329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1189 1325 3967 4173 4377
Size: 1189 136 2642 206 204
Fragments arranged by size:
2642 1189 206 204 136
Bpu10I CC'TnA_GC
Cuts at: 0 3986 4377
Size: 3986 391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 720 4377
Size: 720 3657
BsaI GGTCTCn'nnnn_
Cuts at: 0 1845 1986 4377
Size: 1845 141 2391
Fragments arranged by size:
2391 1845 141
BsaAI yAC'GTr
Cuts at: 0 1134 4377
Size: 1134 3243
BsaBI GATnn'nnATC
Cuts at: 0 1109 4377
Size: 1109 3268
BsaWI w'CCGG_w
Cuts at: 0 2159 4149 4377
Size: 2159 1990 228
Fragments arranged by size:
2159 1990 228
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1043 1073 1931 1961 4377
Size: 1043 30 858 30 2416
Fragments arranged by size:
2416 1043 858 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1064 3193 4209 4377
Size: 1064 2129 1016 168
Fragments arranged by size:
2129 1064 1016 168
BseYI C'CCAG_C
Cuts at: 0 1666 4377
Size: 1666 2711
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2818 3691 4377
Size: 2818 873 686
Fragments arranged by size:
2818 873 686
BsiHKAI G_wGCw'C
Cuts at: 0 1294 3258 3378 3806 3848 3953 4377
Size: 1294 1964 120 428 42 105 424
Fragments arranged by size:
1964 1294 428 424 120 105 42
BsmI GAATG_Cn'
Cuts at: 0 26 3054 4377
Size: 26 3028 1323
Fragments arranged by size:
3028 1323 26
Bsp1286I G_dGCh'C
Cuts at: 0 1294 1546 3258 3378 3806 3848 3953 4377
Size: 1294 252 1712 120 428 42 105 424
Fragments arranged by size:
1712 1294 428 424 252 120 105 42
BspEI T'CCGG_A
Cuts at: 0 4149 4377
Size: 4149 228
BspHI T'CATG_A
Cuts at: 0 170 2526 4377
Size: 170 2356 1851
Fragments arranged by size:
2356 1851 170
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3074 4377
Size: 3074 1303
BsrDI GCAATG_nn'
Cuts at: 0 2237 2672 3565 4377
Size: 2237 435 893 812
Fragments arranged by size:
2237 893 812 435
BstAPI GCAn_nnn'nTGC
Cuts at: 0 937 1906 2773 4377
Size: 937 969 867 1604
Fragments arranged by size:
1604 969 937 867
BstXI CCAn_nnnn'nTGG
Cuts at: 0 770 1635 2205 4377
Size: 770 865 570 2172
Fragments arranged by size:
2172 865 770 570
BstYI r'GATC_y
Cuts at: 0 141 2039 4377
Size: 141 1898 2338
Fragments arranged by size:
2338 1898 141
BstZ17I GTA'TAC
Cuts at: 0 915 2295 4377
Size: 915 1380 2082
Fragments arranged by size:
2082 1380 915
Bsu36I CC'TnA_GG
Cuts at: 0 1478 1538 4377
Size: 1478 60 2839
Fragments arranged by size:
2839 1478 60
BtgI C'CryG_G
Cuts at: 0 2616 3540 3659 4377
Size: 2616 924 119 718
Fragments arranged by size:
2616 924 718 119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 162 4377
Size: 162 4215
BtsI GCAGTG_nn'
Cuts at: 0 165 1106 2249 3891 4377
Size: 165 941 1143 1642 486
Fragments arranged by size:
1642 1143 941 486 165
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2874 2909 4377
Size: 2874 35 1468
Fragments arranged by size:
2874 1468 35
DraI TTT'AAA
Cuts at: 0 2052 4377
Size: 2052 2325
DraIII CAC_nnn'GTG
Cuts at: 0 1575 3038 4377
Size: 1575 1463 1339
Fragments arranged by size:
1575 1463 1339
DrdI GACnn_nn'nnGTC
Cuts at: 0 4051 4377
Size: 4051 326
EaeI y'GGCC_r
Cuts at: 0 4245 4377
Size: 4245 132
EarI CTCTTCn'nnn_
Cuts at: 0 494 890 3131 3208 3533 4377
Size: 494 396 2241 77 325 844
Fragments arranged by size:
2241 844 494 396 325 77
EcoICRI GAG'CTC
Cuts at: 0 3846 3951 4377
Size: 3846 105 426
Fragments arranged by size:
3846 426 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1189 1211 1325 1667 1877 3279 3584
Size: 475 714 22 114 342 210 1402 305
Cuts at: 3584 3967 4097 4173 4240 4377
Size: 383 130 76 67 137
Fragments arranged by size:
1402 714 475 383 342 305 210 137
130 114 76 67 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4191 4377
Size: 4191 186
EcoO109I rG'GnC_Cy
Cuts at: 0 413 483 1282 1542 1543 4377
Size: 413 70 799 260 1 2834
Fragments arranged by size:
2834 799 413 260 70 1
EcoRI G'AATT_C
Cuts at: 0 1884 4377
Size: 1884 2493
EcoRV GAT'ATC
Cuts at: 0 789 4377
Size: 789 3588
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1286 4377
Size: 1286 3091
HaeII r_GCGC'y
Cuts at: 0 185 3119 4377
Size: 185 2934 1258
Fragments arranged by size:
2934 1258 185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 516 548 1931 1963 3183 3215 3901 3933
Size: 516 32 1383 32 1220 32 686 32
Cuts at: 3933 4377
Size: 444
Fragments arranged by size:
1383 1220 686 516 444 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1830 2430 4377
Size: 1830 600 1947
Fragments arranged by size:
1947 1830 600
HindIII A'AGCT_T
Cuts at: 0 1859 2939 4377
Size: 1859 1080 1438
Fragments arranged by size:
1859 1438 1080
MfeI C'AATT_G
Cuts at: 0 425 1016 1823 4377
Size: 425 591 807 2554
Fragments arranged by size:
2554 807 591 425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 2115 2874 3068 4377
Size: 175 1940 759 194 1309
Fragments arranged by size:
1940 1309 759 194 175
MscI TGG'CCA
Cuts at: 0 4247 4377
Size: 4247 130
MslI CAynn'nnrTG
Cuts at: 0 400 768 1210 2242 2554 3713 4377
Size: 400 368 442 1032 312 1159 664
Fragments arranged by size:
1159 1032 664 442 400 368 312
MspA1I CmG'CkG
Cuts at: 0 870 1210 1231 1584 1728 1762 2905 3990
Size: 870 340 21 353 144 34 1143 1085
Cuts at: 3990 4362 4377
Size: 372 15
Fragments arranged by size:
1143 1085 870 372 353 340 144 34
21 15
NcoI C'CATG_G
Cuts at: 0 3659 4377
Size: 3659 718
NdeI CA'TA_TG
Cuts at: 0 1880 4377
Size: 1880 2497
NspI r_CATG'y
Cuts at: 0 1805 2462 2729 4125 4230 4377
Size: 1805 657 267 1396 105 147
Fragments arranged by size:
1805 1396 657 267 147 105
PasI CC'CwG_GG
Cuts at: 0 2412 3940 4377
Size: 2412 1528 437
Fragments arranged by size:
2412 1528 437
PflMI CCAn_nnn'nTGG
Cuts at: 0 349 4237 4377
Size: 349 3888 140
Fragments arranged by size:
3888 349 140
PfoI T'CCnGG_A
Cuts at: 0 348 2006 4189 4377
Size: 348 1658 2183 188
Fragments arranged by size:
2183 1658 348 188
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3360 3392 4377
Size: 3360 32 985
Fragments arranged by size:
3360 985 32
PpuMI rG'GwC_Cy
Cuts at: 0 413 483 4377
Size: 413 70 3894
Fragments arranged by size:
3894 413 70
PsiI TTA'TAA
Cuts at: 0 1031 1775 4377
Size: 1031 744 2602
Fragments arranged by size:
2602 1031 744
PspOMI G'GGCC_C
Cuts at: 0 1542 4377
Size: 1542 2835
PspXI vC'TCGA_Gb
Cuts at: 0 3841 3967 4377
Size: 3841 126 410
Fragments arranged by size:
3841 410 126
PstI C_TGCA'G
Cuts at: 0 501 1101 1733 4377
Size: 501 600 632 2644
Fragments arranged by size:
2644 632 600 501
PvuII CAG'CTG
Cuts at: 0 870 1210 1231 1584 1728 1762 2905 4362
Size: 870 340 21 353 144 34 1143 1457
Cuts at: 4362 4377
Size: 15
Fragments arranged by size:
1457 1143 870 353 340 144 34 21
15
SacI G_AGCT'C
Cuts at: 0 3848 3953 4377
Size: 3848 105 424
Fragments arranged by size:
3848 424 105
SapI GCTCTTCn'nnn_
Cuts at: 0 3131 4377
Size: 3131 1246
SexAI A'CCwGG_T
Cuts at: 0 3148 4377
Size: 3148 1229
SfcI C'TryA_G
Cuts at: 0 137 497 1097 1298 1729 2623 4087 4377
Size: 137 360 600 201 431 894 1464 290
Fragments arranged by size:
1464 894 600 431 360 290 201 137
SmaI CCC'GGG
Cuts at: 0 155 4377
Size: 155 4222
SmlI C'TyrA_G
Cuts at: 0 699 1816 3841 3967 4377
Size: 699 1117 2025 126 410
Fragments arranged by size:
2025 1117 699 410 126
SphI G_CATG'C
Cuts at: 0 2462 2729 4125 4230 4377
Size: 2462 267 1396 105 147
Fragments arranged by size:
2462 1396 267 147 105
SrfI GCCC'GGGC
Cuts at: 0 155 4377
Size: 155 4222
SspI AAT'ATT
Cuts at: 0 684 1380 2001 2735 2839 2854 3015 4377
Size: 684 696 621 734 104 15 161 1362
Fragments arranged by size:
1362 734 696 684 621 161 104 15
StuI AGG'CCT
Cuts at: 0 3320 4377
Size: 3320 1057
StyI C'CwwG_G
Cuts at: 0 1421 3659 4377
Size: 1421 2238 718
Fragments arranged by size:
2238 1421 718
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1563 2197 2544 2648 3096 4377
Size: 1563 634 347 104 448 1281
Fragments arranged by size:
1563 1281 634 448 347 104
TatI w'GTAC_w
Cuts at: 0 2026 2182 4377
Size: 2026 156 2195
Fragments arranged by size:
2195 2026 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 623 655 4075 4107 4377
Size: 623 32 3420 32 270
Fragments arranged by size:
3420 623 270 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3755 4377
Size: 3755 622
XhoI C'TCGA_G
Cuts at: 0 3841 3967 4377
Size: 3841 126 410
Fragments arranged by size:
3841 410 126
XmaI C'CCGG_G
Cuts at: 0 153 4377
Size: 153 4224
XmnI GAAnn'nnTTC
Cuts at: 0 2534 4377
Size: 2534 1843
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AleI AlwNI ApaI
ApaLI ApoI AseI AvaI BaeI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrDI BstAPI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NcoI NdeI NspI PasI PflMI PfoI PpiI
PpuMI PsiI PspOMI PspXI PstI PvuII SacI SapI
SexAI SfcI SmaI SmlI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AhdI AlfI AloI
AscI AsiSI AvrII BamHI BbeI BfrBI BglI BmtI
BplI BsaHI BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII ClaI EagI EciI FseI
FspI FspAI HpaI KasI KpnI MluI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PshAI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SwaI XbaI XcmI ZraI