Restriction map for
pFN21AE9439
(Linear) (Six-base) MAPSORT of: KIEE9439.seq Check: 7890 from: 1 to: 1647
KIEE9439.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 100 345 1647
Size: 100 245 1302
Fragments arranged by size:
1302 245 100
AccI GT'mk_AC
Cuts at: 0 1508 1647
Size: 1508 139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1159 1647
Size: 295 864 488
Fragments arranged by size:
864 488 295
AflIII A'CryG_T
Cuts at: 0 110 1647
Size: 110 1537
AhdI GACnn_n'nnGTC
Cuts at: 0 56 1647
Size: 56 1591
ApaI G_GGCC'C
Cuts at: 0 27 496 1020 1647
Size: 27 469 524 627
Fragments arranged by size:
627 524 469 27
AvaI C'yCGr_G
Cuts at: 0 887 1019 1446 1647
Size: 887 132 427 201
Fragments arranged by size:
887 427 201 132
BanII G_rGCy'C
Cuts at: 0 27 338 496 1020 1647
Size: 27 311 158 524 627
Fragments arranged by size:
627 524 311 158 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 320 594 677 1023 1101 1536 1647
Size: 320 274 83 346 78 435 111
Fragments arranged by size:
435 346 320 274 111 83 78
BbvCI CC'TCA_GC
Cuts at: 0 295 1647
Size: 295 1352
BfrBI ATG'CAT
Cuts at: 0 567 1647
Size: 567 1080
BglI GCCn_nnn'nGGC
Cuts at: 0 1015 1024 1154 1647
Size: 1015 9 130 493
Fragments arranged by size:
1015 493 130 9
BglII A'GATC_T
Cuts at: 0 1367 1647
Size: 1367 280
BlpI GC'TnA_GC
Cuts at: 0 584 1144 1647
Size: 584 560 503
Fragments arranged by size:
584 560 503
Bme1580I G_kGCm'C
Cuts at: 0 27 496 1020 1130 1647
Size: 27 469 524 110 517
Fragments arranged by size:
524 517 469 110 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 1199 1647
Size: 1199 448
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 714 1647
Size: 714 933
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 613 695 1270 1647
Size: 352 261 82 575 377
Fragments arranged by size:
575 377 352 261 82
Bpu10I CC'TnA_GC
Cuts at: 0 295 1647
Size: 295 1352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1625 1647
Size: 1625 22
BsaI GGTCTCn'nnnn_
Cuts at: 0 762 837 1647
Size: 762 75 810
Fragments arranged by size:
810 762 75
BsaAI yAC'GTr
Cuts at: 0 927 1647
Size: 927 720
BsaBI GATnn'nnATC
Cuts at: 0 1180 1647
Size: 1180 467
BsaHI Gr'CG_yC
Cuts at: 0 7 1647
Size: 7 1640
BsaWI w'CCGG_w
Cuts at: 0 1415 1647
Size: 1415 232
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 1590 1647
Size: 28 1562 57
Fragments arranged by size:
1562 57 28
BseYI C'CCAG_C
Cuts at: 0 1013 1157 1437 1647
Size: 1013 144 280 210
Fragments arranged by size:
1013 280 210 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 1647
Size: 180 1467
BsiEI CG_ry'CG
Cuts at: 0 619 1647
Size: 619 1028
BsiHKAI G_wGCw'C
Cuts at: 0 1430 1591 1647
Size: 1430 161 56
Fragments arranged by size:
1430 161 56
Bsp1286I G_dGCh'C
Cuts at: 0 27 338 496 1020 1130 1430 1591 1647
Size: 27 311 158 524 110 300 161 56
Fragments arranged by size:
524 311 300 161 158 110 56 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 100 345 1100 1647
Size: 100 245 755 547
Fragments arranged by size:
755 547 245 100
BsrBI CCG'CTC
Cuts at: 0 1404 1647
Size: 1404 243
BsrDI GCAATG_nn'
Cuts at: 0 192 1647
Size: 192 1455
BstXI CCAn_nnnn'nTGG
Cuts at: 0 18 1647
Size: 18 1629
BstYI r'GATC_y
Cuts at: 0 920 1367 1647
Size: 920 447 280
Fragments arranged by size:
920 447 280
Bsu36I CC'TnA_GG
Cuts at: 0 54 716 1647
Size: 54 662 931
Fragments arranged by size:
931 662 54
BtgI C'CryG_G
Cuts at: 0 701 788 1305 1647
Size: 701 87 517 342
Fragments arranged by size:
701 517 342 87
BtsI GCAGTG_nn'
Cuts at: 0 305 1647
Size: 305 1342
DraIII CAC_nnn'GTG
Cuts at: 0 158 1647
Size: 158 1489
EaeI y'GGCC_r
Cuts at: 0 449 1475 1647
Size: 449 1026 172
Fragments arranged by size:
1026 449 172
EarI CTCTTCn'nnn_
Cuts at: 0 490 1608 1647
Size: 490 1118 39
Fragments arranged by size:
1118 490 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 352 613 695 1159 1270 1647
Size: 295 57 261 82 464 111 377
Fragments arranged by size:
464 377 295 261 111 82 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 876 1647
Size: 876 771
EcoO109I rG'GnC_Cy
Cuts at: 0 15 23 24 492 493 1161 1647
Size: 15 8 1 468 1 668 486
Fragments arranged by size:
668 486 468 15 8 1 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 42 74 1647
Size: 42 32 1573
Fragments arranged by size:
1573 42 32
MscI TGG'CCA
Cuts at: 0 1477 1647
Size: 1477 170
MslI CAynn'nnrTG
Cuts at: 0 1063 1647
Size: 1063 584
MspA1I CmG'CkG
Cuts at: 0 521 1393 1647
Size: 521 872 254
Fragments arranged by size:
872 521 254
NsiI A_TGCA'T
Cuts at: 0 569 1647
Size: 569 1078
NspI r_CATG'y
Cuts at: 0 114 571 1647
Size: 114 457 1076
Fragments arranged by size:
1076 457 114
PasI CC'CwG_GG
Cuts at: 0 11 933 1647
Size: 11 922 714
Fragments arranged by size:
922 714 11
PciI A'CATG_T
Cuts at: 0 110 1647
Size: 110 1537
PmlI CAC'GTG
Cuts at: 0 927 1647
Size: 927 720
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1228 1260 1647
Size: 1228 32 387
Fragments arranged by size:
1228 387 32
PshAI GACnn'nnGTC
Cuts at: 0 470 1647
Size: 470 1177
PspOMI G'GGCC_C
Cuts at: 0 23 492 1016 1647
Size: 23 469 524 631
Fragments arranged by size:
631 524 469 23
PstI C_TGCA'G
Cuts at: 0 312 1647
Size: 312 1335
SfcI C'TryA_G
Cuts at: 0 308 1388 1647
Size: 308 1080 259
Fragments arranged by size:
1080 308 259
SmaI CCC'GGG
Cuts at: 0 889 1021 1647
Size: 889 132 626
Fragments arranged by size:
889 626 132
SmlI C'TyrA_G
Cuts at: 0 1446 1640 1647
Size: 1446 194 7
Fragments arranged by size:
1446 194 7
StyI C'CwwG_G
Cuts at: 0 776 1647
Size: 776 871
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 925 1447 1647
Size: 925 522 200
Fragments arranged by size:
925 522 200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 823 843 1647
Size: 823 20 804
Fragments arranged by size:
823 804 20
XhoI C'TCGA_G
Cuts at: 0 1446 1647
Size: 1446 201
XmaI C'CCGG_G
Cuts at: 0 887 1019 1647
Size: 887 132 628
Fragments arranged by size:
887 628 132
XmnI GAAnn'nnTTC
Cuts at: 0 1369 1647
Size: 1369 278
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI ApaI AvaI BanII
BbsI BbvCI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BstXI BstYI Bsu36I BtgI BtsI DraIII
EaeI EarI Eco57MI EcoNI EcoO109I Hin4I MscI MslI
MspA1I NsiI NspI PasI PciI PmlI PpiI PshAI
PspOMI PstI SfcI SmaI SmlI StyI TaqII XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BcgI BciVI BclI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PflMI
PfoI PmeI PpuMI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
ZraI