Restriction map for
pFN21AE9437
(Linear) (Six-base) MAPSORT of: KIEE9437.seq Check: 5919 from: 1 to: 1089
KIEE9437.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 164 1089
Size: 164 925
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 958 1030 1089
Size: 958 72 59
Fragments arranged by size:
958 72 59
AfeI AGC'GCT
Cuts at: 0 99 679 1089
Size: 99 580 410
Fragments arranged by size:
580 410 99
AleI CACnn'nnGTG
Cuts at: 0 665 1089
Size: 665 424
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 444 1089
Size: 444 645
AlwNI CAG_nnn'CTG
Cuts at: 0 315 402 418 605 1008 1089
Size: 315 87 16 187 403 81
Fragments arranged by size:
403 315 187 87 81 16
ApaI G_GGCC'C
Cuts at: 0 7 1089
Size: 7 1082
ApaLI G'TGCA_C
Cuts at: 0 583 925 997 1089
Size: 583 342 72 92
Fragments arranged by size:
583 342 92 72
AvaI C'yCGr_G
Cuts at: 0 113 484 1089
Size: 113 371 605
Fragments arranged by size:
605 371 113
BanI G'GyrC_C
Cuts at: 0 222 701 1089
Size: 222 479 388
Fragments arranged by size:
479 388 222
BanII G_rGCy'C
Cuts at: 0 7 107 794 1089
Size: 7 100 687 295
Fragments arranged by size:
687 295 100 7
BbeI G_GCGC'C
Cuts at: 0 226 705 1089
Size: 226 479 384
Fragments arranged by size:
479 384 226
BbsI GAAGACnn'nnnn_
Cuts at: 0 22 1089
Size: 22 1067
BbvCI CC'TCA_GC
Cuts at: 0 832 1089
Size: 832 257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 438 472 1089
Size: 438 34 617
Fragments arranged by size:
617 438 34
BlpI GC'TnA_GC
Cuts at: 0 101 283 298 1089
Size: 101 182 15 791
Fragments arranged by size:
791 182 101 15
Bme1580I G_kGCm'C
Cuts at: 0 7 587 800 929 1001 1089
Size: 7 580 213 129 72 88
Fragments arranged by size:
580 213 129 88 72 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 659 813 1089
Size: 659 154 276
Fragments arranged by size:
659 276 154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 1089
Size: 562 527
Bpu10I CC'TnA_GC
Cuts at: 0 491 832 1089
Size: 491 341 257
Fragments arranged by size:
491 341 257
BsaAI yAC'GTr
Cuts at: 0 878 1089
Size: 878 211
BsaHI Gr'CG_yC
Cuts at: 0 125 223 268 348 702 1089
Size: 125 98 45 80 354 387
Fragments arranged by size:
387 354 125 98 80 45
BseYI C'CCAG_C
Cuts at: 0 147 378 954 1026 1089
Size: 147 231 576 72 63
Fragments arranged by size:
576 231 147 72 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 643 1089
Size: 404 239 446
Fragments arranged by size:
446 404 239
BsiEI CG_ry'CG
Cuts at: 0 358 898 1089
Size: 358 540 191
Fragments arranged by size:
540 358 191
BsiHKAI G_wGCw'C
Cuts at: 0 107 563 587 794 929 1001 1089
Size: 107 456 24 207 135 72 88
Fragments arranged by size:
456 207 135 107 88 72 24
Bsp1286I G_dGCh'C
Cuts at: 0 7 107 563 587 794 800 929 1001
Size: 7 100 456 24 207 6 129 72
Cuts at: 1001 1089
Size: 88
Fragments arranged by size:
456 207 129 100 88 72 24 7
6
BspHI T'CATG_A
Cuts at: 0 773 1089
Size: 773 316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 665 1089
Size: 164 501 424
Fragments arranged by size:
501 424 164
BsrDI GCAATG_nn'
Cuts at: 0 731 1089
Size: 731 358
BsrFI r'CCGG_y
Cuts at: 0 367 893 916 929 988 1089
Size: 367 526 23 13 59 101
Fragments arranged by size:
526 367 101 59 23 13
BssSI C'ACGA_G
Cuts at: 0 432 793 1089
Size: 432 361 296
Fragments arranged by size:
432 361 296
BstAPI GCAn_nnn'nTGC
Cuts at: 0 605 933 1005 1089
Size: 605 328 72 84
Fragments arranged by size:
605 328 84 72
BstXI CCAn_nnnn'nTGG
Cuts at: 0 72 244 1089
Size: 72 172 845
Fragments arranged by size:
845 172 72
Bsu36I CC'TnA_GG
Cuts at: 0 120 1089
Size: 120 969
BtgI C'CryG_G
Cuts at: 0 50 533 1089
Size: 50 483 556
Fragments arranged by size:
556 483 50
BtsI GCAGTG_nn'
Cuts at: 0 326 331 603 1089
Size: 326 5 272 486
Fragments arranged by size:
486 326 272 5
DraIII CAC_nnn'GTG
Cuts at: 0 591 1089
Size: 591 498
EaeI y'GGCC_r
Cuts at: 0 47 863 895 914 986 1089
Size: 47 816 32 19 72 103
Fragments arranged by size:
816 103 72 47 32 19
EagI C'GGCC_G
Cuts at: 0 895 1089
Size: 895 194
EarI CTCTTCn'nnn_
Cuts at: 0 934 1006 1089
Size: 934 72 83
Fragments arranged by size:
934 83 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 225 802 880 892 1089
Size: 225 577 78 12 197
Fragments arranged by size:
577 225 197 78 12
EcoICRI GAG'CTC
Cuts at: 0 105 792 1089
Size: 105 687 297
Fragments arranged by size:
687 297 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 958 1030 1089
Size: 562 396 72 59
Fragments arranged by size:
562 396 72 59
EcoO109I rG'GnC_Cy
Cuts at: 0 4 1089
Size: 4 1085
HaeII r_GCGC'y
Cuts at: 0 19 58 101 172 226 283 298 419
Size: 19 39 43 71 54 57 15 121
Cuts at: 419 455 640 681 705 1089
Size: 36 185 41 24 384
Fragments arranged by size:
384 185 121 71 57 54 43 41
39 36 24 19 15
KasI G'GCGC_C
Cuts at: 0 222 701 1089
Size: 222 479 388
Fragments arranged by size:
479 388 222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 296 1089
Size: 89 207 793
Fragments arranged by size:
793 207 89
MslI CAynn'nnrTG
Cuts at: 0 665 1089
Size: 665 424
MspA1I CmG'CkG
Cuts at: 0 552 1005 1089
Size: 552 453 84
Fragments arranged by size:
552 453 84
NaeI GCC'GGC
Cuts at: 0 369 895 918 990 1089
Size: 369 526 23 72 99
Fragments arranged by size:
526 369 99 72 23
NarI GG'CG_CC
Cuts at: 0 223 702 1089
Size: 223 479 387
Fragments arranged by size:
479 387 223
NgoMIV G'CCGG_C
Cuts at: 0 367 893 916 988 1089
Size: 367 526 23 72 101
Fragments arranged by size:
526 367 101 72 23
NspI r_CATG'y
Cuts at: 0 180 236 1089
Size: 180 56 853
Fragments arranged by size:
853 180 56
PflMI CCAn_nnn'nTGG
Cuts at: 0 315 534 615 912 1089
Size: 315 219 81 297 177
Fragments arranged by size:
315 297 219 177 81
PfoI T'CCnGG_A
Cuts at: 0 251 1089
Size: 251 838
PspOMI G'GGCC_C
Cuts at: 0 3 1089
Size: 3 1086
PspXI vC'TCGA_Gb
Cuts at: 0 113 1089
Size: 113 976
PstI C_TGCA'G
Cuts at: 0 1079 1089
Size: 1079 10
PvuII CAG'CTG
Cuts at: 0 1005 1089
Size: 1005 84
SacI G_AGCT'C
Cuts at: 0 107 794 1089
Size: 107 687 295
Fragments arranged by size:
687 295 107
SfcI C'TryA_G
Cuts at: 0 93 444 849 1075 1089
Size: 93 351 405 226 14
Fragments arranged by size:
405 351 226 93 14
SfoI GGC'GCC
Cuts at: 0 224 703 1089
Size: 224 479 386
Fragments arranged by size:
479 386 224
SmlI C'TyrA_G
Cuts at: 0 113 1089
Size: 113 976
SphI G_CATG'C
Cuts at: 0 236 1089
Size: 236 853
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 979 1051 1089
Size: 979 72 38
Fragments arranged by size:
979 72 38
Tth111I GACn'n_nGTC
Cuts at: 0 216 622 1089
Size: 216 406 467
Fragments arranged by size:
467 406 216
XhoI C'TCGA_G
Cuts at: 0 113 1089
Size: 113 976
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BlpI
Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BssSI
BstAPI BstXI Bsu36I BtgI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I HaeII KasI MmeI
MslI MspA1I NaeI NarI NgoMIV NspI PflMI PfoI
PspOMI PspXI PstI PvuII SacI SfcI SfoI SmlI
SphI TaqII Tth111I XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI
BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsiWI BsmI
BsmBI BspEI BsrBI BsrGI BssHII BstBI BstEII BstYI
BstZ17I BtgZI ClaI CspCI DraI DrdI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MscI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI StyI
SwaI TatI TstI XbaI XcmI XmaI XmnI ZraI