Restriction map for
pFN21AE9436
(Linear) (Six-base) MAPSORT of: KIEE9436.seq Check: 7850 from: 1 to: 3051
KIEE9436.orf
With 182 enzymes: SgfI *
March 22, 2012 18:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 159 1560 3051
Size: 159 1401 1491
Fragments arranged by size:
1491 1401 159
Acc65I G'GTAC_C
Cuts at: 0 2232 3051
Size: 2232 819
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 688 902 1402 3051
Size: 62 626 214 500 1649
Fragments arranged by size:
1649 626 500 214 62
AflIII A'CryG_T
Cuts at: 0 2954 3051
Size: 2954 97
AhdI GACnn_n'nnGTC
Cuts at: 0 1322 3051
Size: 1322 1729
AlwNI CAG_nnn'CTG
Cuts at: 0 2092 3051
Size: 2092 959
ApoI r'AATT_y
Cuts at: 0 2209 2302 2709 2784 3051
Size: 2209 93 407 75 267
Fragments arranged by size:
2209 407 267 93 75
AvaI C'yCGr_G
Cuts at: 0 794 1680 3051
Size: 794 886 1371
Fragments arranged by size:
1371 886 794
BamHI G'GATC_C
Cuts at: 0 720 1566 2290 3051
Size: 720 846 724 761
Fragments arranged by size:
846 761 724 720
BanI G'GyrC_C
Cuts at: 0 433 789 1006 2232 3051
Size: 433 356 217 1226 819
Fragments arranged by size:
1226 819 433 356 217
BanII G_rGCy'C
Cuts at: 0 482 2469 3051
Size: 482 1987 582
Fragments arranged by size:
1987 582 482
BbsI GAAGACnn'nnnn_
Cuts at: 0 79 2025 3051
Size: 79 1946 1026
Fragments arranged by size:
1946 1026 79
BciVI GTATCCnnnnn_n'
Cuts at: 0 659 3051
Size: 659 2392
BclI T'GATC_A
Cuts at: 0 1202 1355 1592 3051
Size: 1202 153 237 1459
Fragments arranged by size:
1459 1202 237 153
BfrBI ATG'CAT
Cuts at: 0 298 595 3051
Size: 298 297 2456
Fragments arranged by size:
2456 298 297
BglI GCCn_nnn'nGGC
Cuts at: 0 32 1255 3051
Size: 32 1223 1796
Fragments arranged by size:
1796 1223 32
BlpI GC'TnA_GC
Cuts at: 0 839 3051
Size: 839 2212
BmrI ACTGGGnnnn_n'
Cuts at: 0 778 2470 2533 3051
Size: 778 1692 63 518
Fragments arranged by size:
1692 778 518 63
BmtI G_CTAG'C
Cuts at: 0 1519 3051
Size: 1519 1532
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1602 3051
Size: 1602 1449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 464 830 1198 1597 2739 3051
Size: 182 282 366 368 399 1142 312
Fragments arranged by size:
1142 399 368 366 312 282 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1150 3051
Size: 1150 1901
BsaI GGTCTCn'nnnn_
Cuts at: 0 2124 2901 3051
Size: 2124 777 150
Fragments arranged by size:
2124 777 150
BsaAI yAC'GTr
Cuts at: 0 751 3051
Size: 751 2300
BsaHI Gr'CG_yC
Cuts at: 0 305 3051
Size: 305 2746
BsaWI w'CCGG_w
Cuts at: 0 1967 2319 3051
Size: 1967 352 732
Fragments arranged by size:
1967 732 352
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 999 1029 1425 1455 3051
Size: 999 30 396 30 1596
Fragments arranged by size:
1596 999 396 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 174 1757 3051
Size: 174 1583 1294
Fragments arranged by size:
1583 1294 174
BseYI C'CCAG_C
Cuts at: 0 911 2468 3051
Size: 911 1557 583
Fragments arranged by size:
1557 911 583
BsiHKAI G_wGCw'C
Cuts at: 0 482 3051
Size: 482 2569
BsmI GAATG_Cn'
Cuts at: 0 597 1371 2146 3051
Size: 597 774 775 905
Fragments arranged by size:
905 775 774 597
Bsp1286I G_dGCh'C
Cuts at: 0 482 2469 3051
Size: 482 1987 582
Fragments arranged by size:
1987 582 482
BspEI T'CCGG_A
Cuts at: 0 1967 3051
Size: 1967 1084
BspHI T'CATG_A
Cuts at: 0 1503 3051
Size: 1503 1548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 159 722 1560 3051
Size: 159 563 838 1491
Fragments arranged by size:
1491 838 563 159
BsrDI GCAATG_nn'
Cuts at: 0 354 2671 2988 3051
Size: 354 2317 317 63
Fragments arranged by size:
2317 354 317 63
BsrGI T'GTAC_A
Cuts at: 0 559 3051
Size: 559 2492
BssSI C'ACGA_G
Cuts at: 0 2046 3051
Size: 2046 1005
BstXI CCAn_nnnn'nTGG
Cuts at: 0 751 786 3051
Size: 751 35 2265
Fragments arranged by size:
2265 751 35
BstYI r'GATC_y
Cuts at: 0 720 1566 1964 2290 3051
Size: 720 846 398 326 761
Fragments arranged by size:
846 761 720 398 326
Bsu36I CC'TnA_GG
Cuts at: 0 2494 3051
Size: 2494 557
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1463 3051
Size: 1463 1588
BtsI GCAGTG_nn'
Cuts at: 0 25 645 2824 3051
Size: 25 620 2179 227
Fragments arranged by size:
2179 620 227 25
DraI TTT'AAA
Cuts at: 0 2744 3051
Size: 2744 307
EaeI y'GGCC_r
Cuts at: 0 24 2642 3051
Size: 24 2618 409
Fragments arranged by size:
2618 409 24
EarI CTCTTCn'nnn_
Cuts at: 0 683 981 1475 2784 3051
Size: 683 298 494 1309 267
Fragments arranged by size:
1309 683 494 298 267
EcoICRI GAG'CTC
Cuts at: 0 480 3051
Size: 480 2571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 182 464 688 830 902 1198 1402
Size: 62 120 282 224 142 72 296 204
Cuts at: 1402 1597 2739 3051
Size: 195 1142 312
Fragments arranged by size:
1142 312 296 282 224 204 195 142
120 72 62
EcoO109I rG'GnC_Cy
Cuts at: 0 816 3051
Size: 816 2235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 527 3051
Size: 527 2524
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 359 391 1187 1219 1308 1340 2661 2693
Size: 359 32 796 32 89 32 1321 32
Cuts at: 2693 3051
Size: 358
Fragments arranged by size:
1321 796 359 358 89 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1068 1107 1697 2568 3051
Size: 1068 39 590 871 483
Fragments arranged by size:
1068 871 590 483 39
HindIII A'AGCT_T
Cuts at: 0 237 700 1821 3027 3051
Size: 237 463 1121 1206 24
Fragments arranged by size:
1206 1121 463 237 24
HpaI GTT'AAC
Cuts at: 0 1697 2568 3051
Size: 1697 871 483
Fragments arranged by size:
1697 871 483
KpnI G_GTAC'C
Cuts at: 0 2236 3051
Size: 2236 815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 3051
Size: 674 2377
MscI TGG'CCA
Cuts at: 0 26 2644 3051
Size: 26 2618 407
Fragments arranged by size:
2618 407 26
MslI CAynn'nnrTG
Cuts at: 0 749 784 949 2086 2127 2131 2365 3051
Size: 749 35 165 1137 41 4 234 686
Fragments arranged by size:
1137 749 686 234 165 41 35 4
MspA1I CmG'CkG
Cuts at: 0 1299 2001 2239 3051
Size: 1299 702 238 812
Fragments arranged by size:
1299 812 702 238
NdeI CA'TA_TG
Cuts at: 0 2153 3051
Size: 2153 898
NheI G'CTAG_C
Cuts at: 0 1515 3051
Size: 1515 1536
NsiI A_TGCA'T
Cuts at: 0 300 597 3051
Size: 300 297 2454
Fragments arranged by size:
2454 300 297
NspI r_CATG'y
Cuts at: 0 599 954 1245 2412 2958 3051
Size: 599 355 291 1167 546 93
Fragments arranged by size:
1167 599 546 355 291 93
PciI A'CATG_T
Cuts at: 0 2954 3051
Size: 2954 97
PfoI T'CCnGG_A
Cuts at: 0 2039 3051
Size: 2039 1012
PmlI CAC'GTG
Cuts at: 0 751 3051
Size: 751 2300
PpuMI rG'GwC_Cy
Cuts at: 0 816 3051
Size: 816 2235
PsiI TTA'TAA
Cuts at: 0 520 3051
Size: 520 2531
PstI C_TGCA'G
Cuts at: 0 719 3051
Size: 719 2332
PvuII CAG'CTG
Cuts at: 0 1299 2001 2239 3051
Size: 1299 702 238 812
Fragments arranged by size:
1299 812 702 238
SacI G_AGCT'C
Cuts at: 0 482 3051
Size: 482 2569
SapI GCTCTTCn'nnn_
Cuts at: 0 2784 3051
Size: 2784 267
SbfI CC_TGCA'GG
Cuts at: 0 719 3051
Size: 719 2332
SexAI A'CCwGG_T
Cuts at: 0 2027 3051
Size: 2027 1024
SfcI C'TryA_G
Cuts at: 0 278 530 715 1294 1806 2879 3051
Size: 278 252 185 579 512 1073 172
Fragments arranged by size:
1073 579 512 278 252 185 172
SmlI C'TyrA_G
Cuts at: 0 1129 3051
Size: 1129 1922
StuI AGG'CCT
Cuts at: 0 2075 3051
Size: 2075 976
StyI C'CwwG_G
Cuts at: 0 1166 2763 3051
Size: 1166 1597 288
Fragments arranged by size:
1597 1166 288
TatI w'GTAC_w
Cuts at: 0 559 2850 3051
Size: 559 2291 201
Fragments arranged by size:
2291 559 201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 999 1031 1073 1105 3051
Size: 999 32 42 32 1946
Fragments arranged by size:
1946 999 42 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1102 3051
Size: 1102 1949
XmnI GAAnn'nnTTC
Cuts at: 0 701 2874 3012 3051
Size: 701 2173 138 39
Fragments arranged by size:
2173 701 138 39
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AlwNI ApoI AvaI
BamHI BanI BanII BbsI BciVI BclI BfrBI BglI
BlpI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrDI BsrGI BssSI BstXI BstYI
Bsu36I BtgZI BtsI DraI EaeI EarI EcoICRI Eco57MI
EcoO109I FalI Hin4I HincII HindIII HpaI KpnI MmeI
MscI MslI MspA1I NdeI NheI NsiI NspI PciI
PfoI PmlI PpuMI PsiI PstI PvuII SacI SapI
SbfI SexAI SfcI SmlI StuI StyI TatI TstI
Tth111I XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BglII Bme1580I BmgBI Bpu10I BsaBI
BsgI BsiEI BsiWI BsmBI BsrBI BsrFI BssHII BstAPI
BstBI BstEII BstZ17I BtgI ClaI CspCI DraIII DrdI
EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII KasI MfeI MluI NaeI NarI NcoI NgoMIV
NotI NruI PacI PasI PflMI PmeI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII XbaI XcmI XhoI
XmaI ZraI