Restriction map for
pFN21AE9435
(Linear) (Six-base) MAPSORT of: KIEE9435.seq Check: 8992 from: 1 to: 1851
KIEE9435.orf
With 182 enzymes: SgfI *
March 30, 2011 17:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 538 1851
Size: 538 1313
AccI GT'mk_AC
Cuts at: 0 122 1310 1851
Size: 122 1188 541
Fragments arranged by size:
1188 541 122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 1458 1851
Size: 402 1056 393
Fragments arranged by size:
1056 402 393
AflIII A'CryG_T
Cuts at: 0 488 1851
Size: 488 1363
ApaI G_GGCC'C
Cuts at: 0 8 1712 1851
Size: 8 1704 139
Fragments arranged by size:
1704 139 8
ApoI r'AATT_y
Cuts at: 0 180 897 1851
Size: 180 717 954
Fragments arranged by size:
954 717 180
AvaI C'yCGr_G
Cuts at: 0 1633 1851
Size: 1633 218
BanI G'GyrC_C
Cuts at: 0 499 749 938 1851
Size: 499 250 189 913
Fragments arranged by size:
913 499 250 189
BanII G_rGCy'C
Cuts at: 0 8 887 1544 1616 1712 1851
Size: 8 879 657 72 96 139
Fragments arranged by size:
879 657 139 96 72 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 552 1851
Size: 552 1299
BbvCI CC'TCA_GC
Cuts at: 0 692 1851
Size: 692 1159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 177 211 570 604 1624 1658 1851
Size: 177 34 359 34 1020 34 193
Fragments arranged by size:
1020 359 193 177 34 34 34
BlpI GC'TnA_GC
Cuts at: 0 1538 1851
Size: 1538 313
Bme1580I G_kGCm'C
Cuts at: 0 8 754 1020 1056 1712 1851
Size: 8 746 266 36 656 139
Fragments arranged by size:
746 656 266 139 36 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1707 1851
Size: 1707 144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 1756 1851
Size: 385 1371 95
Fragments arranged by size:
1371 385 95
Bpu10I CC'TnA_GC
Cuts at: 0 692 1157 1316 1586 1610 1851
Size: 692 465 159 270 24 241
Fragments arranged by size:
692 465 270 241 159 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1237 1851
Size: 1237 614
BsaAI yAC'GTr
Cuts at: 0 624 1851
Size: 624 1227
BsaBI GATnn'nnATC
Cuts at: 0 106 1851
Size: 106 1745
BsaWI w'CCGG_w
Cuts at: 0 176 1094 1851
Size: 176 918 757
Fragments arranged by size:
918 757 176
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 592 721 877 1167 1851
Size: 592 129 156 290 684
Fragments arranged by size:
684 592 290 156 129
BseYI C'CCAG_C
Cuts at: 0 56 278 1705 1851
Size: 56 222 1427 146
Fragments arranged by size:
1427 222 146 56
BsiEI CG_ry'CG
Cuts at: 0 1140 1851
Size: 1140 711
BsiHKAI G_wGCw'C
Cuts at: 0 767 1616 1851
Size: 767 849 235
Fragments arranged by size:
849 767 235
Bsp1286I G_dGCh'C
Cuts at: 0 8 754 767 887 1020 1056 1544 1616
Size: 8 746 13 120 133 36 488 72
Cuts at: 1616 1712 1851
Size: 96 139
Fragments arranged by size:
746 488 139 133 120 96 72 36
13 8
BspEI T'CCGG_A
Cuts at: 0 176 1094 1851
Size: 176 918 757
Fragments arranged by size:
918 757 176
BspHI T'CATG_A
Cuts at: 0 461 1851
Size: 461 1390
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 442 538 1851
Size: 442 96 1313
Fragments arranged by size:
1313 442 96
BsrDI GCAATG_nn'
Cuts at: 0 1626 1646 1851
Size: 1626 20 205
Fragments arranged by size:
1626 205 20
BsrFI r'CCGG_y
Cuts at: 0 72 1851
Size: 72 1779
BsrGI T'GTAC_A
Cuts at: 0 491 1851
Size: 491 1360
BssSI C'ACGA_G
Cuts at: 0 1037 1851
Size: 1037 814
BstAPI GCAn_nnn'nTGC
Cuts at: 0 434 1851
Size: 434 1417
BstEII G'GTnAC_C
Cuts at: 0 1246 1288 1851
Size: 1246 42 563
Fragments arranged by size:
1246 563 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 645 1734 1851
Size: 645 1089 117
Fragments arranged by size:
1089 645 117
BstYI r'GATC_y
Cuts at: 0 282 413 629 809 1171 1637 1851
Size: 282 131 216 180 362 466 214
Fragments arranged by size:
466 362 282 216 214 180 131
BtgI C'CryG_G
Cuts at: 0 140 1851
Size: 140 1711
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 115 1734 1851
Size: 115 1619 117
Fragments arranged by size:
1619 117 115
BtsI GCAGTG_nn'
Cuts at: 0 746 1547 1592 1851
Size: 746 801 45 259
Fragments arranged by size:
801 746 259 45
DraIII CAC_nnn'GTG
Cuts at: 0 1659 1851
Size: 1659 192
EaeI y'GGCC_r
Cuts at: 0 215 230 1128 1149 1266 1434 1509 1851
Size: 215 15 898 21 117 168 75 342
Fragments arranged by size:
898 342 215 168 117 75 21 15
EarI CTCTTCn'nnn_
Cuts at: 0 900 1851
Size: 900 951
EcoICRI GAG'CTC
Cuts at: 0 1614 1851
Size: 1614 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 402 1458 1756 1851
Size: 385 17 1056 298 95
Fragments arranged by size:
1056 385 298 95 17
EcoO109I rG'GnC_Cy
Cuts at: 0 4 1851
Size: 4 1847
EcoRI G'AATT_C
Cuts at: 0 180 1851
Size: 180 1671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1236 1488 1851
Size: 1236 252 363
Fragments arranged by size:
1236 363 252
FspI TGC'GCA
Cuts at: 0 427 1851
Size: 427 1424
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1129 1161 1851
Size: 1129 32 690
Fragments arranged by size:
1129 690 32
MscI TGG'CCA
Cuts at: 0 232 1130 1151 1268 1436 1511 1851
Size: 232 898 21 117 168 75 340
Fragments arranged by size:
898 340 232 168 117 75 21
MslI CAynn'nnrTG
Cuts at: 0 88 196 718 1851
Size: 88 108 522 1133
Fragments arranged by size:
1133 522 108 88
MspA1I CmG'CkG
Cuts at: 0 860 1126 1705 1734 1851
Size: 860 266 579 29 117
Fragments arranged by size:
860 579 266 117 29
NcoI C'CATG_G
Cuts at: 0 140 1851
Size: 140 1711
NruI TCG'CGA
Cuts at: 0 1028 1851
Size: 1028 823
NspI r_CATG'y
Cuts at: 0 262 492 1851
Size: 262 230 1359
Fragments arranged by size:
1359 262 230
PciI A'CATG_T
Cuts at: 0 488 1851
Size: 488 1363
PflMI CCAn_nnn'nTGG
Cuts at: 0 1111 1851
Size: 1111 740
PspOMI G'GGCC_C
Cuts at: 0 4 1708 1851
Size: 4 1704 143
Fragments arranged by size:
1704 143 4
PstI C_TGCA'G
Cuts at: 0 666 1125 1851
Size: 666 459 726
Fragments arranged by size:
726 666 459
PvuII CAG'CTG
Cuts at: 0 860 1126 1734 1851
Size: 860 266 608 117
Fragments arranged by size:
860 608 266 117
SacI G_AGCT'C
Cuts at: 0 1616 1851
Size: 1616 235
SbfI CC_TGCA'GG
Cuts at: 0 666 1851
Size: 666 1185
SexAI A'CCwGG_T
Cuts at: 0 443 1110 1851
Size: 443 667 741
Fragments arranged by size:
741 667 443
SfcI C'TryA_G
Cuts at: 0 662 1121 1772 1851
Size: 662 459 651 79
Fragments arranged by size:
662 651 459 79
SmlI C'TyrA_G
Cuts at: 0 1252 1851
Size: 1252 599
SphI G_CATG'C
Cuts at: 0 262 1851
Size: 262 1589
StyI C'CwwG_G
Cuts at: 0 140 1232 1851
Size: 140 1092 619
Fragments arranged by size:
1092 619 140
TatI w'GTAC_w
Cuts at: 0 491 1851
Size: 491 1360
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 405 437 1851
Size: 405 32 1414
Fragments arranged by size:
1414 405 32
Tth111I GACn'n_nGTC
Cuts at: 0 1348 1369 1851
Size: 1348 21 482
Fragments arranged by size:
1348 482 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 147 1447 1851
Size: 147 1300 404
Fragments arranged by size:
1300 404 147
Enzymes that do cut:
AarI AccI AcuI AflIII ApaI ApoI AvaI BanI
BanII BbsI BbvCI BcgI BlpI Bme1580I BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI Hin4I MscI MslI MspA1I NcoI NruI NspI
PciI PflMI PspOMI PstI PvuII SacI SbfI SexAI
SfcI SmlI SphI StyI TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BclI BfrBI BglI BglII
BmgBI BmrI BmtI BsaI BsaHI BsaXI BsgI BsiWI
BsmI BsmBI BsrBI BssHII BstBI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EciI EcoNI EcoRV FseI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TaqII XbaI
XhoI XmaI XmnI ZraI